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The map label for this gene is pilT [H]

Identifier: 110835531

GI number: 110835531

Start: 3017946

End: 3018980

Strand: Direct

Name: pilT [H]

Synonym: ABO_2670

Alternate gene names: 110835531

Gene position: 3017946-3018980 (Clockwise)

Preceding gene: 110835526

Following gene: 110835532

Centisome position: 96.72

GC content: 56.33

Gene sequence:

>1035_bases
ATGGATATTACCGAGCTGCTCGCTTTCAGCGCCAAAAACGGCGCCTCTGACCTTCACTTGTCCGCGGGCCTGCCCCCAAT
GATCCGGGTGGATGGCGACGTGCGCCGCATCAACCTGCCCGCCATGGAGCACAAGGAAGTTCATTCATTGATCTATGACA
TCATGAATGACAAGCAGCGCAAAGATTTCGAGGAATTCTTCGAGACGGACTTCTCCTTCGAGGTGCCCGGCGTAGCCCGC
TTCCGGGTAAACGCCTTTAACCACAACCGTGGTGCCGGTGCGGTATTTCGAACCATTCCATCGAAAGTGCTGACCATGGA
AGACTTGGGGATGGGCAAGGTCTTCCAGAATATCTCCGAAATGCCCCGGGGTATCGTGCTAGTCACGGGGCCCACCGGCT
CGGGTAAATCTACCACCCTGGCAGCCATGGTCGATTACATCAACGATTCCCGTTACGAACACATCCTCACCATTGAGGAT
CCTATCGAATTCGTACACGAATCCAAGAAATGTCTAGTCAACCAGCGCGAAGTTCACCGCGACACCCTCGGTTTCTCTGA
AGCCCTGCGCTCGGCACTGCGGGAAGATCCAGACATTATTTTGGTGGGTGAACTGCGGGATCTGGAAACCATCCGCCTGG
CGCTAACGGCCGCAGAAACCGGCCACCTGGTGTTCGGCACGCTGCACACCACCTCCGCGGCTAAAACCATTGACCGAGTA
ATCGACGTGTTCCCCGCCGAAGAAAAATCCATGATCCGCTCAATGCTGTCGGAATCACTGCAGGCGGTGGTGTCACAAAC
GCTGATGAAGAAAAACGGCGGCGGCCGTGTGGCGGCCCACGAAATCATGATGGGCACCCCAGCGATACGGAACCTGATTC
GTGAAGATAAGGTGGCCCAGATGTATTCCGCCATCCAGACCGGCGGTGCATTAGGCATGCAGACGCTGGACCAGTGCCTG
CAAGGGCTGGTGCAGAAAGGCCTGGTGGCCCGTGATGTGGCTCGTGAGAAAGCCAAGAACCCAGATTCTCTGTAA

Upstream 100 bases:

>100_bases
ATGCGACGATTTTCTGAGGAACCCGGCGAAGGCTCCACAACATAATAACAAAGGGTCACCCACGGGGGCTCTTTCCAAGC
CATAACAGCAAGTATAAATC

Downstream 100 bases:

>100_bases
ATAGCCCCGCAACGGCCCAGCCAAGGCGCCGACAGAATCAGTACAGACAGGCCTTAACGCATGGAATTCGAAGAGCTTCT
CAAGTTGATGGTACAGAAAG

Product: type IV pili twitching motility protein PilT

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 344; Mature: 344

Protein sequence:

>344_residues
MDITELLAFSAKNGASDLHLSAGLPPMIRVDGDVRRINLPAMEHKEVHSLIYDIMNDKQRKDFEEFFETDFSFEVPGVAR
FRVNAFNHNRGAGAVFRTIPSKVLTMEDLGMGKVFQNISEMPRGIVLVTGPTGSGKSTTLAAMVDYINDSRYEHILTIED
PIEFVHESKKCLVNQREVHRDTLGFSEALRSALREDPDIILVGELRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
IDVFPAEEKSMIRSMLSESLQAVVSQTLMKKNGGGRVAAHEIMMGTPAIRNLIREDKVAQMYSAIQTGGALGMQTLDQCL
QGLVQKGLVARDVAREKAKNPDSL

Sequences:

>Translated_344_residues
MDITELLAFSAKNGASDLHLSAGLPPMIRVDGDVRRINLPAMEHKEVHSLIYDIMNDKQRKDFEEFFETDFSFEVPGVAR
FRVNAFNHNRGAGAVFRTIPSKVLTMEDLGMGKVFQNISEMPRGIVLVTGPTGSGKSTTLAAMVDYINDSRYEHILTIED
PIEFVHESKKCLVNQREVHRDTLGFSEALRSALREDPDIILVGELRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
IDVFPAEEKSMIRSMLSESLQAVVSQTLMKKNGGGRVAAHEIMMGTPAIRNLIREDKVAQMYSAIQTGGALGMQTLDQCL
QGLVQKGLVARDVAREKAKNPDSL
>Mature_344_residues
MDITELLAFSAKNGASDLHLSAGLPPMIRVDGDVRRINLPAMEHKEVHSLIYDIMNDKQRKDFEEFFETDFSFEVPGVAR
FRVNAFNHNRGAGAVFRTIPSKVLTMEDLGMGKVFQNISEMPRGIVLVTGPTGSGKSTTLAAMVDYINDSRYEHILTIED
PIEFVHESKKCLVNQREVHRDTLGFSEALRSALREDPDIILVGELRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
IDVFPAEEKSMIRSMLSESLQAVVSQTLMKKNGGGRVAAHEIMMGTPAIRNLIREDKVAQMYSAIQTGGALGMQTLDQCL
QGLVQKGLVARDVAREKAKNPDSL

Specific function: May be involved in pilus retraction [H]

COG id: COG2805

COG function: function code NU; Tfp pilus assembly protein, pilus retraction ATPase PilT

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI87082188, Length=327, Percent_Identity=45.565749235474, Blast_Score=286, Evalue=1e-78,
Organism=Escherichia coli, GI1789723, Length=349, Percent_Identity=31.8051575931232, Blast_Score=137, Evalue=1e-33,
Organism=Escherichia coli, GI1786296, Length=210, Percent_Identity=37.6190476190476, Blast_Score=127, Evalue=9e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR006321
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 37993; Mature: 37993

Theoretical pI: Translated: 6.19; Mature: 6.19

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDITELLAFSAKNGASDLHLSAGLPPMIRVDGDVRRINLPAMEHKEVHSLIYDIMNDKQR
CCHHHHHHHHCCCCCCCEEECCCCCCEEEECCCEEEECCCCHHHHHHHHHHHHHHCCHHH
KDFEEFFETDFSFEVPGVARFRVNAFNHNRGAGAVFRTIPSKVLTMEDLGMGKVFQNISE
HHHHHHHCCCCCCCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHH
MPRGIVLVTGPTGSGKSTTLAAMVDYINDSRYEHILTIEDPIEFVHESKKCLVNQREVHR
CCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHH
DTLGFSEALRSALREDPDIILVGELRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
HHCCHHHHHHHHHHCCCCEEEEECCCCHHHHHHEEEECCCCCEEEEEEHHHHHHHHHHHH
IDVFPAEEKSMIRSMLSESLQAVVSQTLMKKNGGGRVAAHEIMMGTPAIRNLIREDKVAQ
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHCCHHHHHHHHHHHHHH
MYSAIQTGGALGMQTLDQCLQGLVQKGLVARDVAREKAKNPDSL
HHHHHHCCCCCCHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MDITELLAFSAKNGASDLHLSAGLPPMIRVDGDVRRINLPAMEHKEVHSLIYDIMNDKQR
CCHHHHHHHHCCCCCCCEEECCCCCCEEEECCCEEEECCCCHHHHHHHHHHHHHHCCHHH
KDFEEFFETDFSFEVPGVARFRVNAFNHNRGAGAVFRTIPSKVLTMEDLGMGKVFQNISE
HHHHHHHCCCCCCCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHH
MPRGIVLVTGPTGSGKSTTLAAMVDYINDSRYEHILTIEDPIEFVHESKKCLVNQREVHR
CCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHH
DTLGFSEALRSALREDPDIILVGELRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
HHCCHHHHHHHHHHCCCCEEEEECCCCHHHHHHEEEECCCCCEEEEEEHHHHHHHHHHHH
IDVFPAEEKSMIRSMLSESLQAVVSQTLMKKNGGGRVAAHEIMMGTPAIRNLIREDKVAQ
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHCCHHHHHHHHHHHHHH
MYSAIQTGGALGMQTLDQCLQGLVQKGLVARDVAREKAKNPDSL
HHHHHHCCCCCCHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1676385; 10984043 [H]