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The map label for this gene is metK

Identifier: 110835478

GI number: 110835478

Start: 2961291

End: 2962439

Strand: Direct

Name: metK

Synonym: ABO_2617

Alternate gene names: 110835478

Gene position: 2961291-2962439 (Clockwise)

Preceding gene: 110835477

Following gene: 110835479

Centisome position: 94.91

GC content: 57.53

Gene sequence:

>1149_bases
ATGAGTGAATATTCCATCTTTACCTCCGAGTCCGTATCCGAAGGCCATCCGGATAAAATGGCTGACCAGATTTCGGATGC
CATTCTGGATGCCATCATCAAAGATGACCCCCACGCCCGCGTGGCGGTAGAAACAATGGTAAAAACCGGCATGGCCGTGG
TTGCCGGTGAAGTGCGCACCAATACCTATGTGGAATTGGAAGACATCGTTCGCCAAGTGATTCTGGATATCGGTTACGAC
AGCTCAGAAAAAGGCTTCGATGGCGCCAGCTGTGCCGTGCTCAACGGCATTGGCAAACAGTCCGCCGACATTGCTATCGG
TGTAGACGAAGCGGAAGAGAAAGAAATGGGTGCCGGCGACCAAGGCCTGATGTTTGGCTTCGCCACCGACGAAACCGACA
CCCTGATGCCCGCCCCGGTGTACTACTCCCACCGCTTGGTAGAGCGCCAAGCCAAGCTGCGCAAGAACAGCACCCTGCCT
TGGTTACGCCCAGATGCGAAAAGCCAGGTGACCTTGCGTTATGACAACGGCAAGCCGGTAGCTGTGGATGCGGTAGTACT
CTCCACCCAGCACTCCCCGGACATCAAGCTCGCGGATCTGCGTGAAGCGGTGATGGAAGAAATCATCAAGCCGGTGCTGC
CCGAAGAGTGGCTGCACAAGGACACCCTGTACCACGTGAACCCCACCGGGATTTTCGTGATCGGCGGCCCCATGGGCGAC
TGCGGCCTCACCGGCCGCAAGATCATCGTCGACACCTACGGCGGCATGGCCCGCCACGGCGGCGGCGCCTTCTCTGGCAA
GGACCCGTCCAAGGTGGATCGTTCCGCTGCTTATGCCGGACGTTACGTGGCCAAGAACATTGTCGCCGCGGGCTTGGCCA
GCAAGTGCGAGATTCAGGTGAGCTATGCCATTGGTGTGGCCGAGCCCACGTCTATCTCGGTAAATACCTTTGGCACCGGC
AAAATTAGCGATAGCGCCATCGTCGACCTGGTACGCGAACACTTTGATCTGCGCCCACGCGGCATTATTGAAATGCTCGA
CCTACGCAAGCCGATCTATCGCCCCACTGCCAGCTACGGCCACTTCGGCCGCGAAGGCTTCAGTTGGGAAAAAACCGACA
AAACCGAAGCGCTTAAAGCAGCACTTTAA

Upstream 100 bases:

>100_bases
CGCACCTTCAGGAAGAGTTGAGCTTTACACTTTCAACTCATTTTTTTCGCGCCTATTGAAGGCAACGCAACGATCAATGA
ATCACGGGAGCACTCCAGTA

Downstream 100 bases:

>100_bases
ACGCTTACCTGCACACAAACACCGCAGGCAGGCATACCTAACACCTTGGGGCTCGCCACACAGCGAGCCGACTAGCACCA
CGTAATGCGTTTAGCATGTT

Product: S-adenosylmethionine synthetase

Products: NA

Alternate protein names: AdoMet synthase; MAT; Methionine adenosyltransferase

Number of amino acids: Translated: 382; Mature: 381

Protein sequence:

>382_residues
MSEYSIFTSESVSEGHPDKMADQISDAILDAIIKDDPHARVAVETMVKTGMAVVAGEVRTNTYVELEDIVRQVILDIGYD
SSEKGFDGASCAVLNGIGKQSADIAIGVDEAEEKEMGAGDQGLMFGFATDETDTLMPAPVYYSHRLVERQAKLRKNSTLP
WLRPDAKSQVTLRYDNGKPVAVDAVVLSTQHSPDIKLADLREAVMEEIIKPVLPEEWLHKDTLYHVNPTGIFVIGGPMGD
CGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAAYAGRYVAKNIVAAGLASKCEIQVSYAIGVAEPTSISVNTFGTG
KISDSAIVDLVREHFDLRPRGIIEMLDLRKPIYRPTASYGHFGREGFSWEKTDKTEALKAAL

Sequences:

>Translated_382_residues
MSEYSIFTSESVSEGHPDKMADQISDAILDAIIKDDPHARVAVETMVKTGMAVVAGEVRTNTYVELEDIVRQVILDIGYD
SSEKGFDGASCAVLNGIGKQSADIAIGVDEAEEKEMGAGDQGLMFGFATDETDTLMPAPVYYSHRLVERQAKLRKNSTLP
WLRPDAKSQVTLRYDNGKPVAVDAVVLSTQHSPDIKLADLREAVMEEIIKPVLPEEWLHKDTLYHVNPTGIFVIGGPMGD
CGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAAYAGRYVAKNIVAAGLASKCEIQVSYAIGVAEPTSISVNTFGTG
KISDSAIVDLVREHFDLRPRGIIEMLDLRKPIYRPTASYGHFGREGFSWEKTDKTEALKAAL
>Mature_381_residues
SEYSIFTSESVSEGHPDKMADQISDAILDAIIKDDPHARVAVETMVKTGMAVVAGEVRTNTYVELEDIVRQVILDIGYDS
SEKGFDGASCAVLNGIGKQSADIAIGVDEAEEKEMGAGDQGLMFGFATDETDTLMPAPVYYSHRLVERQAKLRKNSTLPW
LRPDAKSQVTLRYDNGKPVAVDAVVLSTQHSPDIKLADLREAVMEEIIKPVLPEEWLHKDTLYHVNPTGIFVIGGPMGDC
GLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAAYAGRYVAKNIVAAGLASKCEIQVSYAIGVAEPTSISVNTFGTGK
ISDSAIVDLVREHFDLRPRGIIEMLDLRKPIYRPTASYGHFGREGFSWEKTDKTEALKAAL

Specific function: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the e

COG id: COG0192

COG function: function code H; S-adenosylmethionine synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AdoMet synthase family

Homologues:

Organism=Homo sapiens, GI4557737, Length=370, Percent_Identity=56.4864864864865, Blast_Score=414, Evalue=1e-116,
Organism=Homo sapiens, GI5174529, Length=370, Percent_Identity=55.4054054054054, Blast_Score=410, Evalue=1e-115,
Organism=Escherichia coli, GI1789311, Length=376, Percent_Identity=72.0744680851064, Blast_Score=566, Evalue=1e-163,
Organism=Caenorhabditis elegans, GI17538498, Length=371, Percent_Identity=56.3342318059299, Blast_Score=418, Evalue=1e-117,
Organism=Caenorhabditis elegans, GI17538494, Length=377, Percent_Identity=55.7029177718833, Blast_Score=416, Evalue=1e-116,
Organism=Caenorhabditis elegans, GI25145633, Length=374, Percent_Identity=55.8823529411765, Blast_Score=416, Evalue=1e-116,
Organism=Caenorhabditis elegans, GI17551082, Length=376, Percent_Identity=54.2553191489362, Blast_Score=407, Evalue=1e-114,
Organism=Caenorhabditis elegans, GI32565905, Length=377, Percent_Identity=50.6631299734748, Blast_Score=355, Evalue=2e-98,
Organism=Caenorhabditis elegans, GI32565911, Length=326, Percent_Identity=53.680981595092, Blast_Score=347, Evalue=5e-96,
Organism=Caenorhabditis elegans, GI25145635, Length=331, Percent_Identity=52.8700906344411, Blast_Score=345, Evalue=3e-95,
Organism=Caenorhabditis elegans, GI32565903, Length=299, Percent_Identity=54.180602006689, Blast_Score=315, Evalue=2e-86,
Organism=Caenorhabditis elegans, GI32565909, Length=89, Percent_Identity=57.3033707865169, Blast_Score=106, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI32565907, Length=50, Percent_Identity=64, Blast_Score=67, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6320710, Length=380, Percent_Identity=53.9473684210526, Blast_Score=402, Evalue=1e-113,
Organism=Saccharomyces cerevisiae, GI6323209, Length=376, Percent_Identity=54.5212765957447, Blast_Score=397, Evalue=1e-111,
Organism=Drosophila melanogaster, GI45552159, Length=377, Percent_Identity=55.9681697612732, Blast_Score=401, Evalue=1e-112,
Organism=Drosophila melanogaster, GI24580529, Length=377, Percent_Identity=55.9681697612732, Blast_Score=401, Evalue=1e-112,
Organism=Drosophila melanogaster, GI24580537, Length=377, Percent_Identity=55.9681697612732, Blast_Score=401, Evalue=1e-112,
Organism=Drosophila melanogaster, GI24580533, Length=377, Percent_Identity=55.9681697612732, Blast_Score=401, Evalue=1e-112,
Organism=Drosophila melanogaster, GI24580535, Length=377, Percent_Identity=55.9681697612732, Blast_Score=401, Evalue=1e-112,
Organism=Drosophila melanogaster, GI24580531, Length=377, Percent_Identity=55.9681697612732, Blast_Score=401, Evalue=1e-112,
Organism=Drosophila melanogaster, GI24580527, Length=376, Percent_Identity=55.3191489361702, Blast_Score=397, Evalue=1e-110,
Organism=Drosophila melanogaster, GI24580525, Length=376, Percent_Identity=55.3191489361702, Blast_Score=397, Evalue=1e-110,
Organism=Drosophila melanogaster, GI24580541, Length=378, Percent_Identity=53.1746031746032, Blast_Score=375, Evalue=1e-104,
Organism=Drosophila melanogaster, GI24580539, Length=117, Percent_Identity=54.7008547008547, Blast_Score=128, Evalue=7e-30,

Paralogues:

None

Copy number: 7,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): METK_ALCBS (Q0VL83)

Other databases:

- EMBL:   AM286690
- RefSeq:   YP_694337.1
- ProteinModelPortal:   Q0VL83
- SMR:   Q0VL83
- STRING:   Q0VL83
- GeneID:   4211504
- GenomeReviews:   AM286690_GR
- KEGG:   abo:ABO_2617
- NMPDR:   fig|393595.12.peg.2624
- eggNOG:   COG0192
- HOGENOM:   HBG443662
- OMA:   IYYAHRL
- PhylomeDB:   Q0VL83
- ProtClustDB:   PRK05250
- BioCyc:   ABOR393595:ABO_2617-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00086
- InterPro:   IPR022631
- InterPro:   IPR022630
- InterPro:   IPR022629
- InterPro:   IPR022628
- InterPro:   IPR002133
- InterPro:   IPR022636
- PANTHER:   PTHR11964
- PIRSF:   PIRSF000497
- TIGRFAMs:   TIGR01034

Pfam domain/function: PF02773 S-AdoMet_synt_C; PF02772 S-AdoMet_synt_M; PF00438 S-AdoMet_synt_N; SSF55973 S-AdoMet_synt

EC number: =2.5.1.6

Molecular weight: Translated: 41427; Mature: 41296

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: PS00376 ADOMET_SYNTHASE_1; PS00377 ADOMET_SYNTHASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEYSIFTSESVSEGHPDKMADQISDAILDAIIKDDPHARVAVETMVKTGMAVVAGEVRT
CCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCEEEECCCCC
NTYVELEDIVRQVILDIGYDSSEKGFDGASCAVLNGIGKQSADIAIGVDEAEEKEMGAGD
CCEEEHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHCCCCCCCCEEEECCCCHHHHCCCCC
QGLMFGFATDETDTLMPAPVYYSHRLVERQAKLRKNSTLPWLRPDAKSQVTLRYDNGKPV
CCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCE
AVDAVVLSTQHSPDIKLADLREAVMEEIIKPVLPEEWLHKDTLYHVNPTGIFVIGGPMGD
EEEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCHHHHCCCCEEEECCCEEEEECCCCCC
CGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAAYAGRYVAKNIVAAGLASKCEIQV
CCCCCCEEEEECCCCCHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEE
SYAIGVAEPTSISVNTFGTGKISDSAIVDLVREHFDLRPRGIIEMLDLRKPIYRPTASYG
EEEEECCCCCEEEEECCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHCCHHCCCCCCCC
HFGREGFSWEKTDKTEALKAAL
CCCCCCCCCCCCCHHHHHHHCC
>Mature Secondary Structure 
SEYSIFTSESVSEGHPDKMADQISDAILDAIIKDDPHARVAVETMVKTGMAVVAGEVRT
CCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCEEEECCCCC
NTYVELEDIVRQVILDIGYDSSEKGFDGASCAVLNGIGKQSADIAIGVDEAEEKEMGAGD
CCEEEHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHCCCCCCCCEEEECCCCHHHHCCCCC
QGLMFGFATDETDTLMPAPVYYSHRLVERQAKLRKNSTLPWLRPDAKSQVTLRYDNGKPV
CCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCE
AVDAVVLSTQHSPDIKLADLREAVMEEIIKPVLPEEWLHKDTLYHVNPTGIFVIGGPMGD
EEEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCHHHHCCCCEEEECCCEEEEECCCCCC
CGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAAYAGRYVAKNIVAAGLASKCEIQV
CCCCCCEEEEECCCCCHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEE
SYAIGVAEPTSISVNTFGTGKISDSAIVDLVREHFDLRPRGIIEMLDLRKPIYRPTASYG
EEEEECCCCCEEEEECCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHCCHHCCCCCCCC
HFGREGFSWEKTDKTEALKAAL
CCCCCCCCCCCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA