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The map label for this gene is ntrB [H]

Identifier: 110835121

GI number: 110835121

Start: 2579425

End: 2580528

Strand: Reverse

Name: ntrB [H]

Synonym: ABO_2260

Alternate gene names: 110835121

Gene position: 2580528-2579425 (Counterclockwise)

Preceding gene: 110835122

Following gene: 110835120

Centisome position: 82.71

GC content: 55.43

Gene sequence:

>1104_bases
ATGGGCCAATTTTTGATGAGTGACATTACGTTGCACAAACGACTACTGGATCACCTGCGTACTGCGGTGATCATGCTGGA
CGAAGCCCTGTGTGTGCGCTACCTGAACCCCGCGGCGGAAATGCTGCTGGCCACCAGCGCGAGCCGTGCGCTGGACCAGC
CTTTGCCTGATTATTTTTTCGACGATGAAGAAGCGCGCCTGGCTATTCAGCAGTGCATTGATGAAGAGCACCCGTTTACC
CAGCGTGAAGCACGGTTGTCGGTGGCACCGGGCCACGAAATTGTGGTGGATTACTCGGTCAGCCCCATCAACGACCCGGG
ACAACCGCTGTGTTTGTTGATGGAGATGCAGTCGCTGGATCGCCTGTTACGCATTGGTCGTGAAGAAGCGTTGATTCATG
CCCACCAAGCCACCCGGGCTCTGGTGCGTGGCGTGGCCCACGAAATCAAAAATCCGCTGGGAGGGATTCGTGGTGCCGCC
CAGTTGTTGGAGCGGGCACTGCCGAGTCCGGACCTGACTGAATACACCAGAGTTATTATCGATGAAGCCGATCGGTTGCG
TTCGGTAGCGGATCGCATGCTTGGCCCGCGTAAACCCCCCGAGTTCCGGGCAGTGAATGTGCACGAGTGCCTCGAGCATG
TGCGTCAGTTGCTGCTGGCGGAATACCCCAAAGGGATAGCTATCTGGCGAGATTACGATATTAGTTTGCCGGATGTGATT
GCCGATCGAGATCAGCTAATTCAAGTGTTGCTCAACCTTATTCGCAATGCCTCCCAGGCGTTGCTGGAGTCACAGACTCC
TGAGCCTCGTATCACTATGCGTACCCGTGTGTTGCGGCAGTTCACCATCGGTGCACAGCGTCATCGACTGGTGCTACGCA
TGGATATTATCGATAACGGTCCGGGGATCACCGAAGAGGTACAGGAGACGATGTTCTATCCCATGGTTAGTGGCCGTGCT
AATGGCACGGGGCTGGGCTTGTCCATTGCGCAATCCATTATTAGTCAGCACCAAGGGCTGATCGAATGCGAAAGCCAGCC
AGGCCAAACTGTATTCAGCATTTTGCTGCCCATGGAGCAGCTGGATGAAACCAACACAGCGTAA

Upstream 100 bases:

>100_bases
AACTCATCAACGACAAGCGCTTGCGCTTGCGCCATAGGCTGCTGGCTGACGTTTTTTATGGCGACTGAGCAGATGGCCTG
ATAATTGCTTATGCCCTGTT

Downstream 100 bases:

>100_bases
TTGCGCGGCTGTGATCAGATTGTTGTCATACACGCCGGCCGCTAGTGCGGAGGTGATAGAAATTACAGGAGTAGCAGGTC
ATGAGTGTCAGTATTTGGGT

Product: sensory box histidine kinase NtrB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 367; Mature: 366

Protein sequence:

>367_residues
MGQFLMSDITLHKRLLDHLRTAVIMLDEALCVRYLNPAAEMLLATSASRALDQPLPDYFFDDEEARLAIQQCIDEEHPFT
QREARLSVAPGHEIVVDYSVSPINDPGQPLCLLMEMQSLDRLLRIGREEALIHAHQATRALVRGVAHEIKNPLGGIRGAA
QLLERALPSPDLTEYTRVIIDEADRLRSVADRMLGPRKPPEFRAVNVHECLEHVRQLLLAEYPKGIAIWRDYDISLPDVI
ADRDQLIQVLLNLIRNASQALLESQTPEPRITMRTRVLRQFTIGAQRHRLVLRMDIIDNGPGITEEVQETMFYPMVSGRA
NGTGLGLSIAQSIISQHQGLIECESQPGQTVFSILLPMEQLDETNTA

Sequences:

>Translated_367_residues
MGQFLMSDITLHKRLLDHLRTAVIMLDEALCVRYLNPAAEMLLATSASRALDQPLPDYFFDDEEARLAIQQCIDEEHPFT
QREARLSVAPGHEIVVDYSVSPINDPGQPLCLLMEMQSLDRLLRIGREEALIHAHQATRALVRGVAHEIKNPLGGIRGAA
QLLERALPSPDLTEYTRVIIDEADRLRSVADRMLGPRKPPEFRAVNVHECLEHVRQLLLAEYPKGIAIWRDYDISLPDVI
ADRDQLIQVLLNLIRNASQALLESQTPEPRITMRTRVLRQFTIGAQRHRLVLRMDIIDNGPGITEEVQETMFYPMVSGRA
NGTGLGLSIAQSIISQHQGLIECESQPGQTVFSILLPMEQLDETNTA
>Mature_366_residues
GQFLMSDITLHKRLLDHLRTAVIMLDEALCVRYLNPAAEMLLATSASRALDQPLPDYFFDDEEARLAIQQCIDEEHPFTQ
REARLSVAPGHEIVVDYSVSPINDPGQPLCLLMEMQSLDRLLRIGREEALIHAHQATRALVRGVAHEIKNPLGGIRGAAQ
LLERALPSPDLTEYTRVIIDEADRLRSVADRMLGPRKPPEFRAVNVHECLEHVRQLLLAEYPKGIAIWRDYDISLPDVIA
DRDQLIQVLLNLIRNASQALLESQTPEPRITMRTRVLRQFTIGAQRHRLVLRMDIIDNGPGITEEVQETMFYPMVSGRAN
GTGLGLSIAQSIISQHQGLIECESQPGQTVFSILLPMEQLDETNTA

Specific function: NtrB acts as a signal transducer which responds to the nitrogen level of cell and modulates the activity of ntrC. In nitrogen limitation ntrB activates ntrC by phosphorylating it, while in nitrogen excess ntrC is dephosphorylated and consequently inactiva

COG id: COG3852

COG function: function code T; Signal transduction histidine kinase, nitrogen specific

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1790300, Length=352, Percent_Identity=45.7386363636364, Blast_Score=281, Evalue=7e-77,
Organism=Escherichia coli, GI1788549, Length=371, Percent_Identity=29.1105121293801, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI1790436, Length=238, Percent_Identity=32.3529411764706, Blast_Score=110, Evalue=1e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 41276; Mature: 41145

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGQFLMSDITLHKRLLDHLRTAVIMLDEALCVRYLNPAAEMLLATSASRALDQPLPDYFF
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCCCCCC
DDEEARLAIQQCIDEEHPFTQREARLSVAPGHEIVVDYSVSPINDPGQPLCLLMEMQSLD
CCHHHHHHHHHHHCCCCCCCHHHCEEEECCCCEEEEEEECCCCCCCCCHHHHHHHHHHHH
RLLRIGREEALIHAHQATRALVRGVAHEIKNPLGGIRGAAQLLERALPSPDLTEYTRVII
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
DEADRLRSVADRMLGPRKPPEFRAVNVHECLEHVRQLLLAEYPKGIAIWRDYDISLPDVI
HHHHHHHHHHHHHCCCCCCCCCCEECHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHH
ADRDQLIQVLLNLIRNASQALLESQTPEPRITMRTRVLRQFTIGAQRHRLVLRMDIIDNG
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCHHHCEEEEEEEEECCC
PGITEEVQETMFYPMVSGRANGTGLGLSIAQSIISQHQGLIECESQPGQTVFSILLPMEQ
CCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHCHHH
LDETNTA
HCCCCCC
>Mature Secondary Structure 
GQFLMSDITLHKRLLDHLRTAVIMLDEALCVRYLNPAAEMLLATSASRALDQPLPDYFF
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCCCCCC
DDEEARLAIQQCIDEEHPFTQREARLSVAPGHEIVVDYSVSPINDPGQPLCLLMEMQSLD
CCHHHHHHHHHHHCCCCCCCHHHCEEEECCCCEEEEEEECCCCCCCCCHHHHHHHHHHHH
RLLRIGREEALIHAHQATRALVRGVAHEIKNPLGGIRGAAQLLERALPSPDLTEYTRVII
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
DEADRLRSVADRMLGPRKPPEFRAVNVHECLEHVRQLLLAEYPKGIAIWRDYDISLPDVI
HHHHHHHHHHHHHCCCCCCCCCCEECHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHH
ADRDQLIQVLLNLIRNASQALLESQTPEPRITMRTRVLRQFTIGAQRHRLVLRMDIIDNG
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCHHHCEEEEEEEEECCC
PGITEEVQETMFYPMVSGRANGTGLGLSIAQSIISQHQGLIECESQPGQTVFSILLPMEQ
CCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHCHHH
LDETNTA
HCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2574025 [H]