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The map label for this gene is ntrC [H]

Identifier: 110835120

GI number: 110835120

Start: 2577911

End: 2579344

Strand: Reverse

Name: ntrC [H]

Synonym: ABO_2259

Alternate gene names: 110835120

Gene position: 2579344-2577911 (Counterclockwise)

Preceding gene: 110835121

Following gene: 110835119

Centisome position: 82.67

GC content: 54.25

Gene sequence:

>1434_bases
ATGAGTGTCAGTATTTGGGTTGTGGATGATGACCGCTCGATTCGCTGGGTGTTGGAAAAGGCGCTGGGCCAGGAAGGCTA
TGCGGTGACCTGCTTTGAGGATGCGGATGAGGCGCTCAACACCCTGGAGCAGGGAGGGCAGGAGCCAGAAGTGCTTATCA
GTGATGTGCGCATGCCGGGCACTGACGGTTTGCGGATGCTGAAAATCCTGCAGCGTAAAAACCCAGACTTGCCGGTAATC
ATCATGACCGCTCACTCCGATCTGGATTCCGCTGTGGCTTCCTACCAGGGTGGCGCGTTTGAATACTTGCCCAAGCCGTT
TGATGTGGATGAAGCGGTGGCGTTGGTACGTAGAGCGATCAAGCACCGGGAAGAATCCCGTGGCGATTTTCCTGAACCGA
TAGAAGAAGCGCCCACCGAAATTATTGGTGAAGCGCCGGCCATGCAGGAAGTCTTTCGTGCCATCGGTAGGCTCAGCCAT
TCCAACGTGACGGTGCTGATTAATGGACAGTCCGGTACCGGTAAAGAGCTGGTTGCCCGCGCCTTGCATCGTCATTCCTT
ACGACGGGAAAAGAATTTCGTCGCCTTGAATATGGCGGCTATTCCCAAGGACCTGATCGAATCAGAGCTGTTTGGCCATG
AAAAAGGCGCTTTTACTGGCGCTAACAATCAGCGGGTGGGCCGTTTTGAACAGGCTAACGGAGGCACCCTGTTTCTCGAC
GAAATCGGGGATATGCCACTGGAGGCTCAAACACGTTTGTTGCGGGTACTGCAGGAAAGTGAATTCTACCGCGTCGGCGG
AACCACGCCGATCCACGTGGATGTACGCATAATCGCCGCCACCCACCAAGATCTGGAAAAACTGGTGAAGGATGGCGACT
TCCGTGAGGATCTATTTCATCGCTTAAACGTTATCCGCATTCATATTCCCCGCATGGCCGAGCGCCGCGAGGACATCCCG
CGATTAGCCAATTATTTCCTTCAGCGAGCGGGAAGTGAATTGGGGGTAGAGCCGAAAATTCTTCATAAGGACACGGAAAA
ATATCTTGCTGCTCAGGCTTGGCCGGGCAACGTGCGGCAGCTGGAAAATACCTGCCGTTGGTTAACCGTGATGGCTTCTG
GTCGAGAAGTACTCGTGGATGATCTTCCGCCAGAGTTGAAAGAGACTGGCCCAGCGAAAGGCGCCGATATTAGTGGTGAC
TGGCTCAAGTCTTTGCGCAACTGGGCCGACCGTGCGTTGTCTCAGGGTGAGCGAAATATTCTTGAACAGGCCGTGCCCGG
TTTTGAGCGCACCATGATCGAAGTGGCTTTACAGCATACCGGTGGTCGTAAACGCGACGCAGCGGCGCTGTTGGGTTGGG
GTCGAAATACGCTGACGCGTAAGATTAAAGAGCTGGACATGGAAAGTCAGCTTCAGGGGAATGATGAGGACTAA

Upstream 100 bases:

>100_bases
ATGAAACCAACACAGCGTAATTGCGCGGCTGTGATCAGATTGTTGTCATACACGCCGGCCGCTAGTGCGGAGGTGATAGA
AATTACAGGAGTAGCAGGTC

Downstream 100 bases:

>100_bases
AAAACGGACACATATTATGTGCCGGTCTTCAGGTTTTTTATTTTCCAGCCGCCGTTCAGCTGCCAGTGACTGTCCATTTG
GTAGCTCTCGGCATCCTCAG

Product: two-component response regulator NtrC

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 477; Mature: 476

Protein sequence:

>477_residues
MSVSIWVVDDDRSIRWVLEKALGQEGYAVTCFEDADEALNTLEQGGQEPEVLISDVRMPGTDGLRMLKILQRKNPDLPVI
IMTAHSDLDSAVASYQGGAFEYLPKPFDVDEAVALVRRAIKHREESRGDFPEPIEEAPTEIIGEAPAMQEVFRAIGRLSH
SNVTVLINGQSGTGKELVARALHRHSLRREKNFVALNMAAIPKDLIESELFGHEKGAFTGANNQRVGRFEQANGGTLFLD
EIGDMPLEAQTRLLRVLQESEFYRVGGTTPIHVDVRIIAATHQDLEKLVKDGDFREDLFHRLNVIRIHIPRMAERREDIP
RLANYFLQRAGSELGVEPKILHKDTEKYLAAQAWPGNVRQLENTCRWLTVMASGREVLVDDLPPELKETGPAKGADISGD
WLKSLRNWADRALSQGERNILEQAVPGFERTMIEVALQHTGGRKRDAAALLGWGRNTLTRKIKELDMESQLQGNDED

Sequences:

>Translated_477_residues
MSVSIWVVDDDRSIRWVLEKALGQEGYAVTCFEDADEALNTLEQGGQEPEVLISDVRMPGTDGLRMLKILQRKNPDLPVI
IMTAHSDLDSAVASYQGGAFEYLPKPFDVDEAVALVRRAIKHREESRGDFPEPIEEAPTEIIGEAPAMQEVFRAIGRLSH
SNVTVLINGQSGTGKELVARALHRHSLRREKNFVALNMAAIPKDLIESELFGHEKGAFTGANNQRVGRFEQANGGTLFLD
EIGDMPLEAQTRLLRVLQESEFYRVGGTTPIHVDVRIIAATHQDLEKLVKDGDFREDLFHRLNVIRIHIPRMAERREDIP
RLANYFLQRAGSELGVEPKILHKDTEKYLAAQAWPGNVRQLENTCRWLTVMASGREVLVDDLPPELKETGPAKGADISGD
WLKSLRNWADRALSQGERNILEQAVPGFERTMIEVALQHTGGRKRDAAALLGWGRNTLTRKIKELDMESQLQGNDED
>Mature_476_residues
SVSIWVVDDDRSIRWVLEKALGQEGYAVTCFEDADEALNTLEQGGQEPEVLISDVRMPGTDGLRMLKILQRKNPDLPVII
MTAHSDLDSAVASYQGGAFEYLPKPFDVDEAVALVRRAIKHREESRGDFPEPIEEAPTEIIGEAPAMQEVFRAIGRLSHS
NVTVLINGQSGTGKELVARALHRHSLRREKNFVALNMAAIPKDLIESELFGHEKGAFTGANNQRVGRFEQANGGTLFLDE
IGDMPLEAQTRLLRVLQESEFYRVGGTTPIHVDVRIIAATHQDLEKLVKDGDFREDLFHRLNVIRIHIPRMAERREDIPR
LANYFLQRAGSELGVEPKILHKDTEKYLAAQAWPGNVRQLENTCRWLTVMASGREVLVDDLPPELKETGPAKGADISGDW
LKSLRNWADRALSQGERNILEQAVPGFERTMIEVALQHTGGRKRDAAALLGWGRNTLTRKIKELDMESQLQGNDED

Specific function: Member of the two-component regulatory system ntrB/ntrC involved in the activation of nitrogen assimilatory genes such as glnA. NtrC is phosphorylated by ntrB and interacts with sigma-54 [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=466, Percent_Identity=66.3090128755365, Blast_Score=622, Evalue=1e-179,
Organism=Escherichia coli, GI1788550, Length=475, Percent_Identity=41.0526315789474, Blast_Score=347, Evalue=8e-97,
Organism=Escherichia coli, GI1790437, Length=462, Percent_Identity=41.7748917748918, Blast_Score=324, Evalue=6e-90,
Organism=Escherichia coli, GI1788905, Length=370, Percent_Identity=44.0540540540541, Blast_Score=293, Evalue=1e-80,
Organism=Escherichia coli, GI1789233, Length=339, Percent_Identity=42.1828908554572, Blast_Score=250, Evalue=2e-67,
Organism=Escherichia coli, GI1789087, Length=257, Percent_Identity=50.9727626459144, Blast_Score=245, Evalue=5e-66,
Organism=Escherichia coli, GI87082117, Length=338, Percent_Identity=42.3076923076923, Blast_Score=236, Evalue=2e-63,
Organism=Escherichia coli, GI87082152, Length=246, Percent_Identity=45.5284552845528, Blast_Score=209, Evalue=5e-55,
Organism=Escherichia coli, GI1786524, Length=337, Percent_Identity=39.7626112759644, Blast_Score=207, Evalue=1e-54,
Organism=Escherichia coli, GI1787583, Length=360, Percent_Identity=35, Blast_Score=194, Evalue=1e-50,
Organism=Escherichia coli, GI87081872, Length=302, Percent_Identity=37.7483443708609, Blast_Score=189, Evalue=4e-49,
Organism=Escherichia coli, GI1789828, Length=229, Percent_Identity=37.5545851528384, Blast_Score=139, Evalue=3e-34,
Organism=Escherichia coli, GI87081858, Length=306, Percent_Identity=31.6993464052288, Blast_Score=134, Evalue=1e-32,
Organism=Escherichia coli, GI1790860, Length=131, Percent_Identity=32.824427480916, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1789809, Length=122, Percent_Identity=28.6885245901639, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1786911, Length=131, Percent_Identity=32.0610687022901, Blast_Score=66, Evalue=6e-12,
Organism=Escherichia coli, GI1786599, Length=123, Percent_Identity=31.7073170731707, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI1790552, Length=127, Percent_Identity=35.4330708661417, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR010114
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 53454; Mature: 53323

Theoretical pI: Translated: 5.14; Mature: 5.14

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVSIWVVDDDRSIRWVLEKALGQEGYAVTCFEDADEALNTLEQGGQEPEVLISDVRMPG
CCEEEEEEECCCHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHCCCCHHHHHHCCCCCC
TDGLRMLKILQRKNPDLPVIIMTAHSDLDSAVASYQGGAFEYLPKPFDVDEAVALVRRAI
CCHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHCCCCHHCCCCCCCHHHHHHHHHHHH
KHREESRGDFPEPIEEAPTEIIGEAPAMQEVFRAIGRLSHSNVTVLINGQSGTGKELVAR
HHHHHHCCCCCCHHHHCCHHHHCCCHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHH
ALHRHSLRREKNFVALNMAAIPKDLIESELFGHEKGAFTGANNQRVGRFEQANGGTLFLD
HHHHHHHHHCCCEEEEEHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCCEECCCCCEEEEH
EIGDMPLEAQTRLLRVLQESEFYRVGGTTPIHVDVRIIAATHQDLEKLVKDGDFREDLFH
HHCCCCHHHHHHHHHHHHHCCEEECCCCCEEEEEEEEEEHHHHHHHHHHHCCCHHHHHHH
RLNVIRIHIPRMAERREDIPRLANYFLQRAGSELGVEPKILHKDTEKYLAAQAWPGNVRQ
HHCEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHH
LENTCRWLTVMASGREVLVDDLPPELKETGPAKGADISGDWLKSLRNWADRALSQGERNI
HHHHHHEEHEECCCCEEEECCCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
LEQAVPGFERTMIEVALQHTGGRKRDAAALLGWGRNTLTRKIKELDMESQLQGNDED
HHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHCCHHHHHHHHHHHCHHHHCCCCCCC
>Mature Secondary Structure 
SVSIWVVDDDRSIRWVLEKALGQEGYAVTCFEDADEALNTLEQGGQEPEVLISDVRMPG
CEEEEEEECCCHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHCCCCHHHHHHCCCCCC
TDGLRMLKILQRKNPDLPVIIMTAHSDLDSAVASYQGGAFEYLPKPFDVDEAVALVRRAI
CCHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHCCCCHHCCCCCCCHHHHHHHHHHHH
KHREESRGDFPEPIEEAPTEIIGEAPAMQEVFRAIGRLSHSNVTVLINGQSGTGKELVAR
HHHHHHCCCCCCHHHHCCHHHHCCCHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHH
ALHRHSLRREKNFVALNMAAIPKDLIESELFGHEKGAFTGANNQRVGRFEQANGGTLFLD
HHHHHHHHHCCCEEEEEHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCCEECCCCCEEEEH
EIGDMPLEAQTRLLRVLQESEFYRVGGTTPIHVDVRIIAATHQDLEKLVKDGDFREDLFH
HHCCCCHHHHHHHHHHHHHCCEEECCCCCEEEEEEEEEEHHHHHHHHHHHCCCHHHHHHH
RLNVIRIHIPRMAERREDIPRLANYFLQRAGSELGVEPKILHKDTEKYLAAQAWPGNVRQ
HHCEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHH
LENTCRWLTVMASGREVLVDDLPPELKETGPAKGADISGDWLKSLRNWADRALSQGERNI
HHHHHHEEHEECCCCEEEECCCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
LEQAVPGFERTMIEVALQHTGGRKRDAAALLGWGRNTLTRKIKELDMESQLQGNDED
HHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHCCHHHHHHHHHHHCHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]