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The map label for this gene is opuE [H]

Identifier: 110835003

GI number: 110835003

Start: 2446986

End: 2448383

Strand: Reverse

Name: opuE [H]

Synonym: ABO_2142

Alternate gene names: 110835003

Gene position: 2448383-2446986 (Counterclockwise)

Preceding gene: 110835004

Following gene: 110835002

Centisome position: 78.47

GC content: 56.8

Gene sequence:

>1398_bases
ATGGGTGTCACCGCGATCAGCTTTCTGTGCGTGCTGGCGTTATTTCTGATTATCGGCATTTCCTCGATCCGGAAAAAACA
TGAGGACGTTAACGATTATCTGACAGCGGGGTATAACACCCCGGCGTGGATCATCGGGCTGTCTTCCGTGGCCACCAATA
ACTCCGGGTACATGTTTATCGGCCTGGTTGGCTATACCTGGGTAGCCGGTGTGTCGGCCTTCTGGGTGGCGCTAGGCTGG
TTAGTGGGGGACTGGTTTGTTTGGCGAGGGTTCCATCGTCGCCTGCGAGAGTATTCGGAAACCCATCCGGTCAATACCAT
CCCCGCTCTCACCTCCCAGAACCCTGAGTTGGCTTCCAGCCCCCTGCGTATTTTGTCTGCACTGCTGACCCTAATATTCC
TGTCGGTTTATGCGGCTGCGCAGCTCAAGGCGGGAAGCAAGGCGTTGCATGTTATGTTTGACTGGCCAATTTGGCTGGGT
GCGGTATTGGGTGCGGTCGTGGTGGTGCTGTATTCCTTTGCCGGTGGTTTACGGGCGTCTATTTGGACTGATGTAGCCCA
GTCGTTTGTGATGTTGTTTGGCATGGTACTGTTGTGCATTGTTGCCATGATCAACGTGGGTTTTCCGTGGACGCTGGTCG
ATGAGCTGCGCAACATTAACCCGGCCCTGCTGGATTGGACCCCGGATAACTTGGCCTTTGGCACCGGTGCTTTTGTACTG
GGCTGGGTGGCGGCGGGCATTGGCGCTATTGGTCAGCCGCATATTTTAGTGCGTACCATTGCGTTACGCAGCCCGGACGA
CGTCCCGGCGGCCCGCAAGGTGTACTTTGCCTGGTACCTGCCCTTTGCGCTTCTGACCGTGACCATCAGTCTCTACGCCC
GCGTACTACTCACAGGAGATCTGTCTGATCCGGAGCTGACACTGCCCACCATGGCTCAGGCTTATTTGCCTGATGTGCTG
GCCGGCATGGTGCTGGCGGCCATTTTTGCCGCCACCTTGTCCACGGCGGATTCCATGTTACTGTCCTGCTCAGCGGCGAT
CAGCCAGGATTTGATTCCCCGGTTCGGGCAATCCTATGTCCGAACCAAAATAGCGACCTTGGGCATTACCTTAGTGGTGC
TGGTGGTGGCGCTGGCCGCGCCGGCTAATGTGTTTACGCTGGTAGTGTTCGCGTGGTCGTCTCTGGGGGGCACCTTGGGG
CCGGTGATTGTGCTGCGGGTGATGAATGCCCGCCTGACTCGGACCACGGCGTTGGTGATGATGCTTGCGGGTTTCACTAC
ACTGATTACCTGGCAGGCGCTTGGTTATTCTGAATCCCTGTATGAATTGTTGCCGGCCCTGGTGGTGTCTTTTGGGGTTT
ATGCCGTGATGCGGCCTTTCTACCGGAAGGAGGAGTGA

Upstream 100 bases:

>100_bases
GCCCGACTAGACTGTCGCTAATAAATTGATATGGGGCAGCCCCCGCGGTGTGAGACGGGTGTATGCTGGATCGTAAATTC
AACTGAGTTCGGTTTAGCGT

Downstream 100 bases:

>100_bases
ATGCCACTGTCCAATCCCGAGCAAGACAAGCGTAAAGCGCTGTATGCGGTAGACCCCTTGGTGCCGCTTGATCAGCTGGA
TCCCGCCACCGACCTGATCG

Product: sodium solute transporter family protein

Products: NA

Alternate protein names: Sodium/proline symporter [H]

Number of amino acids: Translated: 465; Mature: 464

Protein sequence:

>465_residues
MGVTAISFLCVLALFLIIGISSIRKKHEDVNDYLTAGYNTPAWIIGLSSVATNNSGYMFIGLVGYTWVAGVSAFWVALGW
LVGDWFVWRGFHRRLREYSETHPVNTIPALTSQNPELASSPLRILSALLTLIFLSVYAAAQLKAGSKALHVMFDWPIWLG
AVLGAVVVVLYSFAGGLRASIWTDVAQSFVMLFGMVLLCIVAMINVGFPWTLVDELRNINPALLDWTPDNLAFGTGAFVL
GWVAAGIGAIGQPHILVRTIALRSPDDVPAARKVYFAWYLPFALLTVTISLYARVLLTGDLSDPELTLPTMAQAYLPDVL
AGMVLAAIFAATLSTADSMLLSCSAAISQDLIPRFGQSYVRTKIATLGITLVVLVVALAAPANVFTLVVFAWSSLGGTLG
PVIVLRVMNARLTRTTALVMMLAGFTTLITWQALGYSESLYELLPALVVSFGVYAVMRPFYRKEE

Sequences:

>Translated_465_residues
MGVTAISFLCVLALFLIIGISSIRKKHEDVNDYLTAGYNTPAWIIGLSSVATNNSGYMFIGLVGYTWVAGVSAFWVALGW
LVGDWFVWRGFHRRLREYSETHPVNTIPALTSQNPELASSPLRILSALLTLIFLSVYAAAQLKAGSKALHVMFDWPIWLG
AVLGAVVVVLYSFAGGLRASIWTDVAQSFVMLFGMVLLCIVAMINVGFPWTLVDELRNINPALLDWTPDNLAFGTGAFVL
GWVAAGIGAIGQPHILVRTIALRSPDDVPAARKVYFAWYLPFALLTVTISLYARVLLTGDLSDPELTLPTMAQAYLPDVL
AGMVLAAIFAATLSTADSMLLSCSAAISQDLIPRFGQSYVRTKIATLGITLVVLVVALAAPANVFTLVVFAWSSLGGTLG
PVIVLRVMNARLTRTTALVMMLAGFTTLITWQALGYSESLYELLPALVVSFGVYAVMRPFYRKEE
>Mature_464_residues
GVTAISFLCVLALFLIIGISSIRKKHEDVNDYLTAGYNTPAWIIGLSSVATNNSGYMFIGLVGYTWVAGVSAFWVALGWL
VGDWFVWRGFHRRLREYSETHPVNTIPALTSQNPELASSPLRILSALLTLIFLSVYAAAQLKAGSKALHVMFDWPIWLGA
VLGAVVVVLYSFAGGLRASIWTDVAQSFVMLFGMVLLCIVAMINVGFPWTLVDELRNINPALLDWTPDNLAFGTGAFVLG
WVAAGIGAIGQPHILVRTIALRSPDDVPAARKVYFAWYLPFALLTVTISLYARVLLTGDLSDPELTLPTMAQAYLPDVLA
GMVLAAIFAATLSTADSMLLSCSAAISQDLIPRFGQSYVRTKIATLGITLVVLVVALAAPANVFTLVVFAWSSLGGTLGP
VIVLRVMNARLTRTTALVMMLAGFTTLITWQALGYSESLYELLPALVVSFGVYAVMRPFYRKEE

Specific function: Catalyzes the sodium-dependent uptake of extracellular proline [H]

COG id: COG0591

COG function: function code ER; Na+/proline symporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family [H]

Homologues:

Organism=Homo sapiens, GI310128183, Length=473, Percent_Identity=27.2727272727273, Blast_Score=167, Evalue=3e-41,
Organism=Escherichia coli, GI1787251, Length=441, Percent_Identity=30.3854875283447, Blast_Score=187, Evalue=1e-48,
Organism=Escherichia coli, GI1790503, Length=455, Percent_Identity=25.4945054945055, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI87082237, Length=374, Percent_Identity=27.807486631016, Blast_Score=90, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011851
- InterPro:   IPR001734
- InterPro:   IPR018212
- InterPro:   IPR019900 [H]

Pfam domain/function: PF00474 SSF [H]

EC number: NA

Molecular weight: Translated: 50400; Mature: 50269

Theoretical pI: Translated: 8.04; Mature: 8.04

Prosite motif: PS50283 NA_SOLUT_SYMP_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGVTAISFLCVLALFLIIGISSIRKKHEDVNDYLTAGYNTPAWIIGLSSVATNNSGYMFI
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHEEEHHHHEECCCCEEEE
GLVGYTWVAGVSAFWVALGWLVGDWFVWRGFHRRLREYSETHPVNTIPALTSQNPELASS
EHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHCC
PLRILSALLTLIFLSVYAAAQLKAGSKALHVMFDWPIWLGAVLGAVVVVLYSFAGGLRAS
HHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECHHHHHHHHHHHHHHHHHHHCCHHHH
IWTDVAQSFVMLFGMVLLCIVAMINVGFPWTLVDELRNINPALLDWTPDNLAFGTGAFVL
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCEEECCCCCCCCCHHHHHH
GWVAAGIGAIGQPHILVRTIALRSPDDVPAARKVYFAWYLPFALLTVTISLYARVLLTGD
HHHHHHHCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
LSDPELTLPTMAQAYLPDVLAGMVLAAIFAATLSTADSMLLSCSAAISQDLIPRFGQSYV
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RTKIATLGITLVVLVVALAAPANVFTLVVFAWSSLGGTLGPVIVLRVMNARLTRTTALVM
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
MLAGFTTLITWQALGYSESLYELLPALVVSFGVYAVMRPFYRKEE
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
GVTAISFLCVLALFLIIGISSIRKKHEDVNDYLTAGYNTPAWIIGLSSVATNNSGYMFI
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHEEEHHHHEECCCCEEEE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HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8969499; 9384377 [H]