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The map label for this gene is dacA [H]

Identifier: 110834820

GI number: 110834820

Start: 2239886

End: 2241046

Strand: Direct

Name: dacA [H]

Synonym: ABO_1959

Alternate gene names: 110834820

Gene position: 2239886-2241046 (Clockwise)

Preceding gene: 110834819

Following gene: 110834821

Centisome position: 71.79

GC content: 53.23

Gene sequence:

>1161_bases
ATGCGTCCCCAGTCTTTTCGATTTACCGTTTTAGCTCTCTTTGCTTTATTTGGCACCGCTTTTTCCCTCCTGGCTCAAGC
GCAAATGGTGCCCCGTGCGCCACAAATCGATGCCAAAGGCTACTTGCTGATGGATGCAACCAGCGGCCAAATCATCGTCG
AGCGTAACGCAGATCAACGCCTACCTCCGGCCAGCCTAACAAAGATGATGACAGCCTACATTGCTGAAGCGGAGCTGCAG
AAAGGCAATATTTCCGAGAAAGACATGGCGCCAATCAGCGTTCAGGCTTGGCAAATGGGCGGTTCACGCATGTTCGTACG
CGAAGGGACCCGAGTTCCGGTGGGGGACTTGCTGCGAGGCATCATCATCCAGTCCGGTAACGACGCCAGCGTGGCCATGG
CCGAATACATTGCCGGTTCCGAAGATGCCTTTGCCGATTTGATGAACCAACACGCTAACCGTCTGGGCATGGACAACAGC
CATTTCATGAACGCCACTGGCTGGCCGGCGGAAAACCATTTCACCACCGCCAAGGACATGGCCATTCTAGCCCGCGCCAT
CGTGCGAGATTTTCCCGATCACTACGACCTATACGCCAAAAAGGAATTTACCTACAACGGCATCACTCAACAAAACCGCA
ACCTGCTACTGTGGCGTGACCCCTCTGTGGACGGCCTTAAAACCGGACATACCGATGAAGCCGGCTATTGCCTGGTCTCT
TCCGCTAAAAAAGACGGTATGCGTCTAATCGCCGTGGTCATGGGTACCGATTCTGAAGGCGCACGCGCCCGTGAATCACA
AAAGCTACTGACCTACGGTTTCCGCTTCTTCGAAACCTACAAAGCGTATAGCGCTGGCGATGTCCTTGATACCGTCGATG
TATGGATGGGCGAACAGAACCAACTTCGTCTAGGCCTAGCTGAAGACTTGGTACTGACCATCCCCCGTGAAAGCCATGAA
AGCCTGAAAGCGGAAATCAGTGTTACTCCACAGCTGGAAGCGCCTATCGTGGAAGGCCAACAATACGGCTCCCTCACCGT
CCGCATGAACAATGATGTGCTGGTTAAAAAACCATTGGTGGCATTGGAAGCCGTTGAAGAAGCTGGCCTCTTCACCAAGC
TTTGGCATCACCTTATGCTGTTCTTCAAAGGGCTGTTCTAG

Upstream 100 bases:

>100_bases
CCTTCGGGTTTGCACCGAACAACGACGGCAGGCATATCATTGATTCCCTAAACCCTTCAAAGAGCGAATCATCCTAGAAC
AACCAACAGGCAGGATATCC

Downstream 100 bases:

>100_bases
GAACCTCAGCACGCGACACGCAGGCACACTTACCATGACTCAGGTTTATCTCAACGGCGCCTTCATGGCGCCAGCAGATG
CAACGATATCCCCCATGGAT

Product: D-alanyl-D-alanine-carboxypeptidase

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein 5; PBP-5 [H]

Number of amino acids: Translated: 386; Mature: 386

Protein sequence:

>386_residues
MRPQSFRFTVLALFALFGTAFSLLAQAQMVPRAPQIDAKGYLLMDATSGQIIVERNADQRLPPASLTKMMTAYIAEAELQ
KGNISEKDMAPISVQAWQMGGSRMFVREGTRVPVGDLLRGIIIQSGNDASVAMAEYIAGSEDAFADLMNQHANRLGMDNS
HFMNATGWPAENHFTTAKDMAILARAIVRDFPDHYDLYAKKEFTYNGITQQNRNLLLWRDPSVDGLKTGHTDEAGYCLVS
SAKKDGMRLIAVVMGTDSEGARARESQKLLTYGFRFFETYKAYSAGDVLDTVDVWMGEQNQLRLGLAEDLVLTIPRESHE
SLKAEISVTPQLEAPIVEGQQYGSLTVRMNNDVLVKKPLVALEAVEEAGLFTKLWHHLMLFFKGLF

Sequences:

>Translated_386_residues
MRPQSFRFTVLALFALFGTAFSLLAQAQMVPRAPQIDAKGYLLMDATSGQIIVERNADQRLPPASLTKMMTAYIAEAELQ
KGNISEKDMAPISVQAWQMGGSRMFVREGTRVPVGDLLRGIIIQSGNDASVAMAEYIAGSEDAFADLMNQHANRLGMDNS
HFMNATGWPAENHFTTAKDMAILARAIVRDFPDHYDLYAKKEFTYNGITQQNRNLLLWRDPSVDGLKTGHTDEAGYCLVS
SAKKDGMRLIAVVMGTDSEGARARESQKLLTYGFRFFETYKAYSAGDVLDTVDVWMGEQNQLRLGLAEDLVLTIPRESHE
SLKAEISVTPQLEAPIVEGQQYGSLTVRMNNDVLVKKPLVALEAVEEAGLFTKLWHHLMLFFKGLF
>Mature_386_residues
MRPQSFRFTVLALFALFGTAFSLLAQAQMVPRAPQIDAKGYLLMDATSGQIIVERNADQRLPPASLTKMMTAYIAEAELQ
KGNISEKDMAPISVQAWQMGGSRMFVREGTRVPVGDLLRGIIIQSGNDASVAMAEYIAGSEDAFADLMNQHANRLGMDNS
HFMNATGWPAENHFTTAKDMAILARAIVRDFPDHYDLYAKKEFTYNGITQQNRNLLLWRDPSVDGLKTGHTDEAGYCLVS
SAKKDGMRLIAVVMGTDSEGARARESQKLLTYGFRFFETYKAYSAGDVLDTVDVWMGEQNQLRLGLAEDLVLTIPRESHE
SLKAEISVTPQLEAPIVEGQQYGSLTVRMNNDVLVKKPLVALEAVEEAGLFTKLWHHLMLFFKGLF

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein. Note=N-terminal lies in the periplasmic space (By similarity) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI1786851, Length=390, Percent_Identity=43.8461538461538, Blast_Score=333, Evalue=1e-92,
Organism=Escherichia coli, GI1787062, Length=382, Percent_Identity=43.1937172774869, Blast_Score=323, Evalue=1e-89,
Organism=Escherichia coli, GI87082022, Length=383, Percent_Identity=38.9033942558747, Blast_Score=271, Evalue=7e-74,
Organism=Escherichia coli, GI87082054, Length=247, Percent_Identity=29.1497975708502, Blast_Score=83, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 42900; Mature: 42900

Theoretical pI: Translated: 5.66; Mature: 5.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRPQSFRFTVLALFALFGTAFSLLAQAQMVPRAPQIDAKGYLLMDATSGQIIVERNADQR
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCEEEEECCCCCC
LPPASLTKMMTAYIAEAELQKGNISEKDMAPISVQAWQMGGSRMFVREGTRVPVGDLLRG
CCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCEEEEECCCCCCHHHHHHH
IIIQSGNDASVAMAEYIAGSEDAFADLMNQHANRLGMDNSHFMNATGWPAENHFTTAKDM
HEEECCCCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCEECCCCCCCCCCCCCHHHH
AILARAIVRDFPDHYDLYAKKEFTYNGITQQNRNLLLWRDPSVDGLKTGHTDEAGYCLVS
HHHHHHHHHHCCCHHCCEEECEEEECCCCCCCCCEEEEECCCCCCCCCCCCCCCCEEEEE
SAKKDGMRLIAVVMGTDSEGARARESQKLLTYGFRFFETYKAYSAGDVLDTVDVWMGEQN
CCCCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCC
QLRLGLAEDLVLTIPRESHESLKAEISVTPQLEAPIVEGQQYGSLTVRMNNDVLVKKPLV
CEEEEECCCEEEECCCCCCCCEEEEEEECCCCCCCCCCCCCCCEEEEEECCCEEEECCHH
ALEAVEEAGLFTKLWHHLMLFFKGLF
HHHHHHHCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRPQSFRFTVLALFALFGTAFSLLAQAQMVPRAPQIDAKGYLLMDATSGQIIVERNADQR
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCEEEEECCCCCC
LPPASLTKMMTAYIAEAELQKGNISEKDMAPISVQAWQMGGSRMFVREGTRVPVGDLLRG
CCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCEEEEECCCCCCHHHHHHH
IIIQSGNDASVAMAEYIAGSEDAFADLMNQHANRLGMDNSHFMNATGWPAENHFTTAKDM
HEEECCCCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCEECCCCCCCCCCCCCHHHH
AILARAIVRDFPDHYDLYAKKEFTYNGITQQNRNLLLWRDPSVDGLKTGHTDEAGYCLVS
HHHHHHHHHHCCCHHCCEEECEEEECCCCCCCCCEEEEECCCCCCCCCCCCCCCCEEEEE
SAKKDGMRLIAVVMGTDSEGARARESQKLLTYGFRFFETYKAYSAGDVLDTVDVWMGEQN
CCCCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCC
QLRLGLAEDLVLTIPRESHESLKAEISVTPQLEAPIVEGQQYGSLTVRMNNDVLVKKPLV
CEEEEECCCEEEECCCCCCCCEEEEEEECCCCCCCCCCCCCCCEEEEEECCCEEEECCHH
ALEAVEEAGLFTKLWHHLMLFFKGLF
HHHHHHHCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]