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The map label for this gene is dat [H]

Identifier: 110834821

GI number: 110834821

Start: 2241081

End: 2241965

Strand: Direct

Name: dat [H]

Synonym: ABO_1960

Alternate gene names: 110834821

Gene position: 2241081-2241965 (Clockwise)

Preceding gene: 110834820

Following gene: 110834822

Centisome position: 71.83

GC content: 57.4

Gene sequence:

>885_bases
ATGACTCAGGTTTATCTCAACGGCGCCTTCATGGCGCCAGCAGATGCAACGATATCCCCCATGGATCGGGGCTTTCTGTT
TGCCGACGGGATTTACGAAGTGATACCAGCCTATAACGGCGTATTATTCCGCTTCGAAGAACACCTGATCCGCCTGGAAC
GCTCGCTGGCAGAAGTCGACATTCGCAATCCCCACAGCCGGGCACAGTGGCGAGAACGATGTGAACAACTACTGCGCGCC
AATGGCGGCGGCAATCTCTCGGTTTACCTGCAAGTCACCCGTGGCGCTGCTGAAAAGCGCGATCATGCGTTCCCGTCGCC
CGCCGTCACCCCCACTGTATTCATGATGACCAACCCGATAGCGATTCCTGCCGCCGACAGCCCAGAAACCGCCGTTGGCG
CTCGCGCAATCACCCTGGATGATATTCGCTGGGCCCGTTGCGATATTAAGTCCGTCTCCTTGCTGCCCAACAGCCTACTC
CGCCAACAAGCCGTGGCCGCCGGGGCCAGCGAAGCCATTTTGCTGCGCGACGGCTTTGTTACCGAAGGCTCGGCCAGCAA
TGTCTTCATCGCCAAGGCCGGCACCATTGCGACCCCCCCAAAAAGCCATGCCATTCTTGGCGGCATTACCCGGGATCTGG
TGGTAGAGCTGTGCCACCAGCACGGCCTCGCACTAGAAGAGCGTGAAATCACTGAAATGCAGTTACGCCAAGCAGATGAA
ATCTGGATCACCAGTTCGACCAAGGAAGTGGTGCCCGTAATTCAGCTCAACAACGCTATAATAGGGAATGGAATGCCGGG
GCCATTATGGAAAGCCCTTGCGCATCACTACGTCCAGCATAAACGCCGACTTTGCGGGCTTGATGCCCCAAACGCGCACG
ACTGA

Upstream 100 bases:

>100_bases
GAAGAAGCTGGCCTCTTCACCAAGCTTTGGCATCACCTTATGCTGTTCTTCAAAGGGCTGTTCTAGGAACCTCAGCACGC
GACACGCAGGCACACTTACC

Downstream 100 bases:

>100_bases
CAGGAGCCGCCCATGGCCATTCAGGAACATTTGTGGGAATTCCCCCATGACATGCAGTTAAAAGTCATGGGGGCCATCGA
CTCCCCCCTTGAAGCCGCGC

Product: d-alanine aminotransferase

Products: NA

Alternate protein names: D-amino acid aminotransferase; D-amino acid transaminase; DAAT; D-aspartate aminotransferase [H]

Number of amino acids: Translated: 294; Mature: 293

Protein sequence:

>294_residues
MTQVYLNGAFMAPADATISPMDRGFLFADGIYEVIPAYNGVLFRFEEHLIRLERSLAEVDIRNPHSRAQWRERCEQLLRA
NGGGNLSVYLQVTRGAAEKRDHAFPSPAVTPTVFMMTNPIAIPAADSPETAVGARAITLDDIRWARCDIKSVSLLPNSLL
RQQAVAAGASEAILLRDGFVTEGSASNVFIAKAGTIATPPKSHAILGGITRDLVVELCHQHGLALEEREITEMQLRQADE
IWITSSTKEVVPVIQLNNAIIGNGMPGPLWKALAHHYVQHKRRLCGLDAPNAHD

Sequences:

>Translated_294_residues
MTQVYLNGAFMAPADATISPMDRGFLFADGIYEVIPAYNGVLFRFEEHLIRLERSLAEVDIRNPHSRAQWRERCEQLLRA
NGGGNLSVYLQVTRGAAEKRDHAFPSPAVTPTVFMMTNPIAIPAADSPETAVGARAITLDDIRWARCDIKSVSLLPNSLL
RQQAVAAGASEAILLRDGFVTEGSASNVFIAKAGTIATPPKSHAILGGITRDLVVELCHQHGLALEEREITEMQLRQADE
IWITSSTKEVVPVIQLNNAIIGNGMPGPLWKALAHHYVQHKRRLCGLDAPNAHD
>Mature_293_residues
TQVYLNGAFMAPADATISPMDRGFLFADGIYEVIPAYNGVLFRFEEHLIRLERSLAEVDIRNPHSRAQWRERCEQLLRAN
GGGNLSVYLQVTRGAAEKRDHAFPSPAVTPTVFMMTNPIAIPAADSPETAVGARAITLDDIRWARCDIKSVSLLPNSLLR
QQAVAAGASEAILLRDGFVTEGSASNVFIAKAGTIATPPKSHAILGGITRDLVVELCHQHGLALEEREITEMQLRQADEI
WITSSTKEVVPVIQLNNAIIGNGMPGPLWKALAHHYVQHKRRLCGLDAPNAHD

Specific function: Acts on the D-isomers of alanine, leucine, aspartate, glutamate, aminobutyrate, norvaline and asparagine. The enzyme transfers an amino group from a substrate D-amino acid to the pyridoxal phosphate cofactor to form pyridoxamine and an alpha- keto acid in

COG id: COG0115

COG function: function code EH; Branched-chain amino acid aminotransferase/4-amino-4-deoxychorismate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Escherichia coli, GI48994963, Length=289, Percent_Identity=24.9134948096886, Blast_Score=91, Evalue=1e-19,

Paralogues:

None

Copy number: 2342 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 11,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001544
- InterPro:   IPR018300
- InterPro:   IPR005784 [H]

Pfam domain/function: PF01063 Aminotran_4 [H]

EC number: =2.6.1.21 [H]

Molecular weight: Translated: 32170; Mature: 32039

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: PS00770 AA_TRANSFER_CLASS_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQVYLNGAFMAPADATISPMDRGFLFADGIYEVIPAYNGVLFRFEEHLIRLERSLAEVD
CCEEEEECEEECCCCCCCCCCCCCEEECCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHC
IRNPHSRAQWRERCEQLLRANGGGNLSVYLQVTRGAAEKRDHAFPSPAVTPTVFMMTNPI
CCCCCHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCHHHCCCCCCCCCCCEEEEEECCE
AIPAADSPETAVGARAITLDDIRWARCDIKSVSLLPNSLLRQQAVAAGASEAILLRDGFV
EECCCCCCCHHHCEEEEEECCCEEECCCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCC
TEGSASNVFIAKAGTIATPPKSHAILGGITRDLVVELCHQHGLALEEREITEMQLRQADE
CCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCC
IWITSSTKEVVPVIQLNNAIIGNGMPGPLWKALAHHYVQHKRRLCGLDAPNAHD
EEEECCCCCEEEEEEECCEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
TQVYLNGAFMAPADATISPMDRGFLFADGIYEVIPAYNGVLFRFEEHLIRLERSLAEVD
CEEEEECEEECCCCCCCCCCCCCEEECCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHC
IRNPHSRAQWRERCEQLLRANGGGNLSVYLQVTRGAAEKRDHAFPSPAVTPTVFMMTNPI
CCCCCHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCHHHCCCCCCCCCCCEEEEEECCE
AIPAADSPETAVGARAITLDDIRWARCDIKSVSLLPNSLLRQQAVAAGASEAILLRDGFV
EECCCCCCCHHHCEEEEEECCCEEECCCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCC
TEGSASNVFIAKAGTIATPPKSHAILGGITRDLVVELCHQHGLALEEREITEMQLRQADE
CCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCC
IWITSSTKEVVPVIQLNNAIIGNGMPGPLWKALAHHYVQHKRRLCGLDAPNAHD
EEEECCCCCEEEEEEECCEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9003455 [H]