Click here to switch to the map view.

The map label for this gene is amtB2 [C]

Identifier: 110834337

GI number: 110834337

Start: 1683965

End: 1685275

Strand: Direct

Name: amtB2 [C]

Synonym: ABO_1476

Alternate gene names: 110834337

Gene position: 1683965-1685275 (Clockwise)

Preceding gene: 110834336

Following gene: 110834338

Centisome position: 53.97

GC content: 57.06

Gene sequence:

>1311_bases
ATGCCTCTGCGACGCCACCACTGGGCTTCTCTGCTGTTTATTCCCCAGTTTGCCAATGCGGACACTCTCACCGCTACTGA
TACTGTGTGGATTGCCATCGCCGCGGCGCTGGTTTTTTTCATGCAGGCGGGGTTTGCACTGCTCGAAAGCGGCTTGAGCC
GAGCGAAAAACTCCCTGAATGTAGTGATGAAAAACTATATGGATCTGTGCGTCGGCACTCTGGTTTTCTGGGCCGTCGGT
TTTGGGTTGATGTTCGGCACCAGCAGCAATGGCTGGTTCGGACAGGATCAGTTCATGCTGAACCACGCAGATAGCAGCAC
CTGGGCTATGCTCCTGTTCCAGATTATGTTTGCGGCCACGGCCGCTACTATCGCCTCCGGCGCAATGGCAGAACGAACAC
GCTACGCAGGTTACCTGTGTGGCGCGGTGTTCATTACTGGGGTTATCTATCCATTGTTTGGCAACTGGGCATGGAACGAT
AATGGCTGGTTGGCACAAATGGGCTTTATTGATTTCGCTGGCTCTACTGTGGTTCATAGTATTGGCGGCTGGTGCGCCTT
GGCCGGCATCATGGTAGTCGGCCCCCGCCTGGGCCGTTTCGATAGCCAAGGTACCCCACGCAGCATCCCTGGACACAACC
TTTCCTACGTAGCACTGGGTGGCTTCATTCTTTGGTTCGGCTGGTTTGGCTTTAATGGCGGCTCAACCCTGTCGGCCAGC
GTGGACATTGCCAAAGTGAACCTGAACACCCAACTTGCCGCGGCCGCCGGAGCCGTCGGCAGTGTTATCTTCCGTATGGC
CAAGCGTCACCCGATCTTGCTCACGGAAACAGTGAATGGCAGCTTGGCCGGACTGGTCGCCGTCACCGCCGGCTGTGCCG
TCATGACCCCTAGCTTTGCCATTGTCACCGGCGTGGTTGCCGGCGCACTAGCCGCCTTCGGGGCTCGCATTCTGCTCGCC
TTTCGACTGGATGATGTAGTGGGCGCGGTACCCGTTCACGCCTTTGCCGGTGCGTGGGGCACCCTGGCCGCCGGACTGTT
TTACGACGGCGATCTGTTCAATAGCCAGCGTGTGATGGTGCAACTACTGGGAGTGGTCGTGGCATTTGGCTGGGCATTCT
TTACCGCATTAATCATGTACATGCTGGTGTCTATTTTAGCCGGGCTGAGGGCGCCCGCACTGCACGAACAGCGAGGGCTA
GATCTCAGCGAACATGCTGAAATCGGCTATCCTGAATTCCCCCAAAACACCCTATACAGCAGCGAAAATTATAGCGCAGA
GAAGGCAAGCCGTCAGGAGCCGCGGCCATGA

Upstream 100 bases:

>100_bases
AATGATAAAGAAGTAGAAGAGCCACCCCCAACCGCCCGACATTCTGGCGCAGAGATTGCATACCTGCTTCATTAACCAAT
AATGAATAGGGTTCATAACC

Downstream 100 bases:

>100_bases
ACTTCGTGCCCATAGAGCAAAGTGTTCAGCGTCGACGGCGAGCCGTCATTACTGGCCTAACACCATATATGAATGAACCC
GTGTTACGGGAAGCGATTAG

Product: ammonium transporter

Products: NH3 [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 436; Mature: 435

Protein sequence:

>436_residues
MPLRRHHWASLLFIPQFANADTLTATDTVWIAIAAALVFFMQAGFALLESGLSRAKNSLNVVMKNYMDLCVGTLVFWAVG
FGLMFGTSSNGWFGQDQFMLNHADSSTWAMLLFQIMFAATAATIASGAMAERTRYAGYLCGAVFITGVIYPLFGNWAWND
NGWLAQMGFIDFAGSTVVHSIGGWCALAGIMVVGPRLGRFDSQGTPRSIPGHNLSYVALGGFILWFGWFGFNGGSTLSAS
VDIAKVNLNTQLAAAAGAVGSVIFRMAKRHPILLTETVNGSLAGLVAVTAGCAVMTPSFAIVTGVVAGALAAFGARILLA
FRLDDVVGAVPVHAFAGAWGTLAAGLFYDGDLFNSQRVMVQLLGVVVAFGWAFFTALIMYMLVSILAGLRAPALHEQRGL
DLSEHAEIGYPEFPQNTLYSSENYSAEKASRQEPRP

Sequences:

>Translated_436_residues
MPLRRHHWASLLFIPQFANADTLTATDTVWIAIAAALVFFMQAGFALLESGLSRAKNSLNVVMKNYMDLCVGTLVFWAVG
FGLMFGTSSNGWFGQDQFMLNHADSSTWAMLLFQIMFAATAATIASGAMAERTRYAGYLCGAVFITGVIYPLFGNWAWND
NGWLAQMGFIDFAGSTVVHSIGGWCALAGIMVVGPRLGRFDSQGTPRSIPGHNLSYVALGGFILWFGWFGFNGGSTLSAS
VDIAKVNLNTQLAAAAGAVGSVIFRMAKRHPILLTETVNGSLAGLVAVTAGCAVMTPSFAIVTGVVAGALAAFGARILLA
FRLDDVVGAVPVHAFAGAWGTLAAGLFYDGDLFNSQRVMVQLLGVVVAFGWAFFTALIMYMLVSILAGLRAPALHEQRGL
DLSEHAEIGYPEFPQNTLYSSENYSAEKASRQEPRP
>Mature_435_residues
PLRRHHWASLLFIPQFANADTLTATDTVWIAIAAALVFFMQAGFALLESGLSRAKNSLNVVMKNYMDLCVGTLVFWAVGF
GLMFGTSSNGWFGQDQFMLNHADSSTWAMLLFQIMFAATAATIASGAMAERTRYAGYLCGAVFITGVIYPLFGNWAWNDN
GWLAQMGFIDFAGSTVVHSIGGWCALAGIMVVGPRLGRFDSQGTPRSIPGHNLSYVALGGFILWFGWFGFNGGSTLSASV
DIAKVNLNTQLAAAAGAVGSVIFRMAKRHPILLTETVNGSLAGLVAVTAGCAVMTPSFAIVTGVVAGALAAFGARILLAF
RLDDVVGAVPVHAFAGAWGTLAAGLFYDGDLFNSQRVMVQLLGVVVAFGWAFFTALIMYMLVSILAGLRAPALHEQRGLD
LSEHAEIGYPEFPQNTLYSSENYSAEKASRQEPRP

Specific function: Involved In The Uptake Of Ammonia (Probable). [C]

COG id: COG0004

COG function: function code P; Ammonia permease

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ammonium transporter (TC 2.A.49) family [H]

Homologues:

Organism=Escherichia coli, GI1786656, Length=422, Percent_Identity=31.2796208530806, Blast_Score=154, Evalue=2e-38,
Organism=Caenorhabditis elegans, GI17550182, Length=417, Percent_Identity=35.0119904076739, Blast_Score=213, Evalue=2e-55,
Organism=Caenorhabditis elegans, GI17550180, Length=422, Percent_Identity=32.4644549763033, Blast_Score=213, Evalue=2e-55,
Organism=Caenorhabditis elegans, GI32564042, Length=436, Percent_Identity=31.1926605504587, Blast_Score=195, Evalue=3e-50,
Organism=Caenorhabditis elegans, GI133931100, Length=429, Percent_Identity=29.8368298368298, Blast_Score=183, Evalue=2e-46,
Organism=Saccharomyces cerevisiae, GI6324187, Length=440, Percent_Identity=29.0909090909091, Blast_Score=156, Evalue=8e-39,
Organism=Saccharomyces cerevisiae, GI6325396, Length=418, Percent_Identity=29.1866028708134, Blast_Score=132, Evalue=1e-31,
Organism=Saccharomyces cerevisiae, GI6321559, Length=418, Percent_Identity=27.2727272727273, Blast_Score=125, Evalue=1e-29,
Organism=Drosophila melanogaster, GI161078314, Length=410, Percent_Identity=32.9268292682927, Blast_Score=194, Evalue=8e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001905
- InterPro:   IPR018047 [H]

Pfam domain/function: PF00909 Ammonium_transp [H]

EC number: NA

Molecular weight: Translated: 46577; Mature: 46446

Theoretical pI: Translated: 7.01; Mature: 7.01

Prosite motif: PS01219 AMMONIUM_TRANSP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLRRHHWASLLFIPQFANADTLTATDTVWIAIAAALVFFMQAGFALLESGLSRAKNSLN
CCCCHHCCHHEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VVMKNYMDLCVGTLVFWAVGFGLMFGTSSNGWFGQDQFMLNHADSSTWAMLLFQIMFAAT
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHH
AATIASGAMAERTRYAGYLCGAVFITGVIYPLFGNWAWNDNGWLAQMGFIDFAGSTVVHS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCEEEECCHHHCCHHHHHH
IGGWCALAGIMVVGPRLGRFDSQGTPRSIPGHNLSYVALGGFILWFGWFGFNGGSTLSAS
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHCCCCCCEEEEE
VDIAKVNLNTQLAAAAGAVGSVIFRMAKRHPILLTETVNGSLAGLVAVTAGCAVMTPSFA
EEEEEEECCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHCHHHHCCHHH
IVTGVVAGALAAFGARILLAFRLDDVVGAVPVHAFAGAWGTLAAGLFYDGDLFNSQRVMV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCHHHHH
QLLGVVVAFGWAFFTALIMYMLVSILAGLRAPALHEQRGLDLSEHAEIGYPEFPQNTLYS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCHHHHCCCCCCCCCCCCCCC
SENYSAEKASRQEPRP
CCCCCHHHHCCCCCCC
>Mature Secondary Structure 
PLRRHHWASLLFIPQFANADTLTATDTVWIAIAAALVFFMQAGFALLESGLSRAKNSLN
CCCHHCCHHEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VVMKNYMDLCVGTLVFWAVGFGLMFGTSSNGWFGQDQFMLNHADSSTWAMLLFQIMFAAT
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHH
AATIASGAMAERTRYAGYLCGAVFITGVIYPLFGNWAWNDNGWLAQMGFIDFAGSTVVHS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCEEEECCHHHCCHHHHHH
IGGWCALAGIMVVGPRLGRFDSQGTPRSIPGHNLSYVALGGFILWFGWFGFNGGSTLSAS
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHCCCCCCEEEEE
VDIAKVNLNTQLAAAAGAVGSVIFRMAKRHPILLTETVNGSLAGLVAVTAGCAVMTPSFA
EEEEEEECCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHCHHHHCCHHH
IVTGVVAGALAAFGARILLAFRLDDVVGAVPVHAFAGAWGTLAAGLFYDGDLFNSQRVMV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCHHHHH
QLLGVVVAFGWAFFTALIMYMLVSILAGLRAPALHEQRGLDLSEHAEIGYPEFPQNTLYS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCHHHHCCCCCCCCCCCCCCC
SENYSAEKASRQEPRP
CCCCCHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NH3 [Periplasm] [C]

Specific reaction: NH3 [Periplasm] = NH3 [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]