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The map label for this gene is gacA [H]

Identifier: 110834165

GI number: 110834165

Start: 1478399

End: 1479052

Strand: Direct

Name: gacA [H]

Synonym: ABO_1304

Alternate gene names: 110834165

Gene position: 1478399-1479052 (Clockwise)

Preceding gene: 110834156

Following gene: 110834166

Centisome position: 47.38

GC content: 52.45

Gene sequence:

>654_bases
ATGATCAAAGTATTGGTAGTGGATGACCATGATCTGGTCCGCACCGGCATTGCTCGAATGCTGGACGAAGTTGATGGTTT
GCAGGTTGTCGGGGAAGCGAATTCAGGCGAGCGGGCCATACAGATGGTTCGGGAGTTGGCGCCAGAAGTGGTGCTCATGG
ACCTGAAAATGCCTGGCATCGGTGGGCTAGAAGCCACACGTAAGTTGCTGCGAGTGGATGAGGGAATCCGAGTTATTGCC
GTGACCATGTGTGAAGATGAGCCGTTTCCTTCGCGCTTGATGAAGGCGGGCGCCGCGGGTTATATGACCAAAGGCGCAGA
TTTGGAAGAAATGGTCCGCGCCATCAAGATTGTGGCTACCGGACAGCGCTATATTAGCCCGGAGATTGCCCAGGTTATGG
CGCTCAAGCCCTATTCTGGAGATGAAGGGTCACAGCTGGACATGCTATCCGAACGTGAATTGCAAATCATGATGATGATC
GTCAGTTGTCATAAAGTGCAGGACGTGGCAGATAAGCTCTGCCTCTCACCCAAAACCGTCAATACCTATCGGTACCGAAT
ATTCGATAAGCTGGGCGTGACTAGTGATGTGGAAATGGCGCTTCTAGCGGTGCGCCATGGCATGTTGGATGCAGTGGAAA
ACTCGGTATTCTGA

Upstream 100 bases:

>100_bases
CGTTCTATGCACGTCGATGTACAAGGGGTAGTCTTATGCCTATAAAATCGAAAGAAAAATAGCACACTGGCGGGCAAGGG
AACTGCCCGGGGGCATAGTA

Downstream 100 bases:

>100_bases
CCGCTGCGCCTTACACAGGGGGAAATCGCTAGGGCAGTGCTGATACAATTACCTCGCCACACTGTGTTGAGATGCCCCCT
TGTCTAATTTCGACCCCAAA

Product: DNA-binding response regulator GacA

Products: NA

Alternate protein names: Global activator [H]

Number of amino acids: Translated: 217; Mature: 217

Protein sequence:

>217_residues
MIKVLVVDDHDLVRTGIARMLDEVDGLQVVGEANSGERAIQMVRELAPEVVLMDLKMPGIGGLEATRKLLRVDEGIRVIA
VTMCEDEPFPSRLMKAGAAGYMTKGADLEEMVRAIKIVATGQRYISPEIAQVMALKPYSGDEGSQLDMLSERELQIMMMI
VSCHKVQDVADKLCLSPKTVNTYRYRIFDKLGVTSDVEMALLAVRHGMLDAVENSVF

Sequences:

>Translated_217_residues
MIKVLVVDDHDLVRTGIARMLDEVDGLQVVGEANSGERAIQMVRELAPEVVLMDLKMPGIGGLEATRKLLRVDEGIRVIA
VTMCEDEPFPSRLMKAGAAGYMTKGADLEEMVRAIKIVATGQRYISPEIAQVMALKPYSGDEGSQLDMLSERELQIMMMI
VSCHKVQDVADKLCLSPKTVNTYRYRIFDKLGVTSDVEMALLAVRHGMLDAVENSVF
>Mature_217_residues
MIKVLVVDDHDLVRTGIARMLDEVDGLQVVGEANSGERAIQMVRELAPEVVLMDLKMPGIGGLEATRKLLRVDEGIRVIA
VTMCEDEPFPSRLMKAGAAGYMTKGADLEEMVRAIKIVATGQRYISPEIAQVMALKPYSGDEGSQLDMLSERELQIMMMI
VSCHKVQDVADKLCLSPKTVNTYRYRIFDKLGVTSDVEMALLAVRHGMLDAVENSVF

Specific function: Forms part of a two-component regulatory system gacA/gacA(lemA). May be involved in lesion formation, swarming and in the production of extracellular protease, syringomycin and N- acyl-L-homoserine lactone (acyl-HSL) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1788222, Length=212, Percent_Identity=52.8301886792453, Blast_Score=231, Evalue=4e-62,
Organism=Escherichia coli, GI1790102, Length=200, Percent_Identity=35.5, Blast_Score=115, Evalue=3e-27,
Organism=Escherichia coli, GI1788521, Length=205, Percent_Identity=30.7317073170732, Blast_Score=99, Evalue=2e-22,
Organism=Escherichia coli, GI1786747, Length=196, Percent_Identity=26.530612244898, Blast_Score=97, Evalue=8e-22,
Organism=Escherichia coli, GI1788712, Length=205, Percent_Identity=26.8292682926829, Blast_Score=95, Evalue=5e-21,
Organism=Escherichia coli, GI1787473, Length=182, Percent_Identity=29.1208791208791, Blast_Score=77, Evalue=8e-16,
Organism=Escherichia coli, GI1788546, Length=201, Percent_Identity=24.8756218905473, Blast_Score=68, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR016032
- InterPro:   IPR001789
- InterPro:   IPR000792
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00196 GerE; PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 23959; Mature: 23959

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00622 HTH_LUXR_1 ; PS50043 HTH_LUXR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
7.4 %Met     (Translated Protein)
8.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
7.4 %Met     (Mature Protein)
8.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKVLVVDDHDLVRTGIARMLDEVDGLQVVGEANSGERAIQMVRELAPEVVLMDLKMPGI
CEEEEEECCHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHCCCEEEEEECCCCC
GGLEATRKLLRVDEGIRVIAVTMCEDEPFPSRLMKAGAAGYMTKGADLEEMVRAIKIVAT
CCHHHHHHHHHHCCCCEEEEEEEECCCCCHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHC
GQRYISPEIAQVMALKPYSGDEGSQLDMLSERELQIMMMIVSCHKVQDVADKLCLSPKTV
CCHHCCHHHHHHHEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
NTYRYRIFDKLGVTSDVEMALLAVRHGMLDAVENSVF
CHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MIKVLVVDDHDLVRTGIARMLDEVDGLQVVGEANSGERAIQMVRELAPEVVLMDLKMPGI
CEEEEEECCHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHCCCEEEEEECCCCC
GGLEATRKLLRVDEGIRVIAVTMCEDEPFPSRLMKAGAAGYMTKGADLEEMVRAIKIVAT
CCHHHHHHHHHHCCCCEEEEEEEECCCCCHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHC
GQRYISPEIAQVMALKPYSGDEGSQLDMLSERELQIMMMIVSCHKVQDVADKLCLSPKTV
CCHHCCHHHHHHHEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
NTYRYRIFDKLGVTSDVEMALLAVRHGMLDAVENSVF
CHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8002569 [H]