Click here to switch to the map view.

The map label for this gene is galE [H]

Identifier: 110833770

GI number: 110833770

Start: 1016975

End: 1018015

Strand: Direct

Name: galE [H]

Synonym: ABO_0909

Alternate gene names: 110833770

Gene position: 1016975-1018015 (Clockwise)

Preceding gene: 110833769

Following gene: 110833771

Centisome position: 32.59

GC content: 54.47

Gene sequence:

>1041_bases
ATGACAACCCGCTACGAACAGGTACAGCAAGACCTTAAAGCCAATCCCAAAACCTGGCTCGTCACCGGCGTGGCCGGTTT
TATCGGCTCGAATTTACTCGAAACATTGTTGGGCTTGAACCAAACTGTCATCGGCCTGGACAACTTTGCCACTGGCCACC
AGCACAACCTGGATGAAGTCCAGCAAACCGTCACTGCTGACCAATGGGCCAACTTCACCTTCATTGAAGGCGACATCCGC
AGTCTGGATGACTGCAAAAAAGCGGTCACGGGTGTGGACTACGTACTCCATCAGGCTGCCCTGGGCTCCGTCCCGCGTTC
TATCGAAGACCCAATCACTACTAACCAAGCGAATATCGACGGCTTTCTGAATATGCTAGTGGCCGCCAAAGACGAAAACG
TGGCCAGCTTCACCTACGCGGCCTCCAGTTCTACCTACGGCGACCACCCGGGCCTACCCAAAGTGGAAGACAAAATCGGC
AAGCCCCTTTCCCCTTATGCCGTCACGAAATACGTCAACGAACTCTATGCTGACGTATTTGCCCGCACCTACGATTTCAA
CACCATCGGCTTGCGCTATTTTAACGTGTTCGGCAAACGCCAGGACCCTAACGGTGCCTACGCCGCGGTCATCCCCAAAT
GGACCGCCGCCATGATCCAGGGCGAGGACGTGTTCATCAATGGCGACGGCGAAACCAGCCGGGATTTCTGCTTTATCGAG
AATGTGGTGCAGATAAATCTGTTGGCCGCCACCGCAGAGGCAGAAGCAAAAAACCAGGTCTTCAACGTGGCCTTGGGCGA
CAGAACCACGCTGAACACCCTGTTCGATGCGCTTAAAACAGCCCTGAAAGAGTGTGGGGTGAACTATGAAAGTGATCCTG
TTTACCGTGATTTCCGCGCAGGAGACGTTCGCCACTCCCAGGCCGACATCAGCAAGGCACAAGAGTTGCTGGGCTATGAA
CCCGCCTACCGCATTTACGATGGCATCGGTAAGGCCATGCCCTGGTACGAAAGCACACTGAATACTGGAAGCGACGCATG
A

Upstream 100 bases:

>100_bases
GTGGGGCGGTCGTTCTACCGAGAAAGGAACGTAAGCAGGAAAGTCGCGGCGCAACAACCGCGCCCACAAACAAACTGAAT
CTACGAGAAACTGAATCGCC

Downstream 100 bases:

>100_bases
AAATTCTGGTCACCGGCGGTGCAGGCTTTATCGGTTCCGCCGTTATTCGTCACATCATCAGCAACACGGACGACAGTGTC
GTCAATGTGGACAAGCTCAC

Product: 4-epimerase

Products: UDPglucoseal [C]

Alternate protein names: NA

Number of amino acids: Translated: 346; Mature: 345

Protein sequence:

>346_residues
MTTRYEQVQQDLKANPKTWLVTGVAGFIGSNLLETLLGLNQTVIGLDNFATGHQHNLDEVQQTVTADQWANFTFIEGDIR
SLDDCKKAVTGVDYVLHQAALGSVPRSIEDPITTNQANIDGFLNMLVAAKDENVASFTYAASSSTYGDHPGLPKVEDKIG
KPLSPYAVTKYVNELYADVFARTYDFNTIGLRYFNVFGKRQDPNGAYAAVIPKWTAAMIQGEDVFINGDGETSRDFCFIE
NVVQINLLAATAEAEAKNQVFNVALGDRTTLNTLFDALKTALKECGVNYESDPVYRDFRAGDVRHSQADISKAQELLGYE
PAYRIYDGIGKAMPWYESTLNTGSDA

Sequences:

>Translated_346_residues
MTTRYEQVQQDLKANPKTWLVTGVAGFIGSNLLETLLGLNQTVIGLDNFATGHQHNLDEVQQTVTADQWANFTFIEGDIR
SLDDCKKAVTGVDYVLHQAALGSVPRSIEDPITTNQANIDGFLNMLVAAKDENVASFTYAASSSTYGDHPGLPKVEDKIG
KPLSPYAVTKYVNELYADVFARTYDFNTIGLRYFNVFGKRQDPNGAYAAVIPKWTAAMIQGEDVFINGDGETSRDFCFIE
NVVQINLLAATAEAEAKNQVFNVALGDRTTLNTLFDALKTALKECGVNYESDPVYRDFRAGDVRHSQADISKAQELLGYE
PAYRIYDGIGKAMPWYESTLNTGSDA
>Mature_345_residues
TTRYEQVQQDLKANPKTWLVTGVAGFIGSNLLETLLGLNQTVIGLDNFATGHQHNLDEVQQTVTADQWANFTFIEGDIRS
LDDCKKAVTGVDYVLHQAALGSVPRSIEDPITTNQANIDGFLNMLVAAKDENVASFTYAASSSTYGDHPGLPKVEDKIGK
PLSPYAVTKYVNELYADVFARTYDFNTIGLRYFNVFGKRQDPNGAYAAVIPKWTAAMIQGEDVFINGDGETSRDFCFIEN
VVQINLLAATAEAEAKNQVFNVALGDRTTLNTLFDALKTALKECGVNYESDPVYRDFRAGDVRHSQADISKAQELLGYEP
AYRIYDGIGKAMPWYESTLNTGSDA

Specific function: Galactose metabolism; third step. [C]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family [H]

Homologues:

Organism=Homo sapiens, GI42516563, Length=347, Percent_Identity=27.9538904899135, Blast_Score=105, Evalue=6e-23,
Organism=Homo sapiens, GI7657641, Length=330, Percent_Identity=26.3636363636364, Blast_Score=93, Evalue=4e-19,
Organism=Homo sapiens, GI56237023, Length=323, Percent_Identity=26.0061919504644, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI56118217, Length=323, Percent_Identity=26.0061919504644, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI189083684, Length=323, Percent_Identity=26.0061919504644, Blast_Score=81, Evalue=1e-15,
Organism=Escherichia coli, GI1786974, Length=321, Percent_Identity=29.2834890965732, Blast_Score=111, Evalue=6e-26,
Organism=Escherichia coli, GI1788353, Length=349, Percent_Identity=25.7879656160458, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI48994969, Length=344, Percent_Identity=25.5813953488372, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1790049, Length=321, Percent_Identity=23.98753894081, Blast_Score=67, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI71982035, Length=329, Percent_Identity=29.1793313069909, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI71982038, Length=329, Percent_Identity=29.1793313069909, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17539532, Length=335, Percent_Identity=25.0746268656716, Blast_Score=92, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI17568069, Length=335, Percent_Identity=23.8805970149254, Blast_Score=80, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6319493, Length=354, Percent_Identity=25.1412429378531, Blast_Score=80, Evalue=6e-16,
Organism=Drosophila melanogaster, GI21356223, Length=347, Percent_Identity=25.6484149855908, Blast_Score=101, Evalue=8e-22,
Organism=Drosophila melanogaster, GI19923002, Length=328, Percent_Identity=29.5731707317073, Blast_Score=97, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040
- InterPro:   IPR008089 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: 5.1.3.2 [C]

Molecular weight: Translated: 38079; Mature: 37948

Theoretical pI: Translated: 4.43; Mature: 4.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTRYEQVQQDLKANPKTWLVTGVAGFIGSNLLETLLGLNQTVIGLDNFATGHQHNLDEV
CCCHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCHHHH
QQTVTADQWANFTFIEGDIRSLDDCKKAVTGVDYVLHQAALGSVPRSIEDPITTNQANID
HHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCCCCCCCCHH
GFLNMLVAAKDENVASFTYAASSSTYGDHPGLPKVEDKIGKPLSPYAVTKYVNELYADVF
HHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHH
ARTYDFNTIGLRYFNVFGKRQDPNGAYAAVIPKWTAAMIQGEDVFINGDGETSRDFCFIE
HHHCCCCHHHHHHHHHHCCCCCCCCCEEEECCHHHHHEEECCEEEEECCCCCCCCHHHHH
NVVQINLLAATAEAEAKNQVFNVALGDRTTLNTLFDALKTALKECGVNYESDPVYRDFRA
HHEEEEEEEEHHHHHHHHCEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
GDVRHSQADISKAQELLGYEPAYRIYDGIGKAMPWYESTLNTGSDA
CCCCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHCCCCCCC
>Mature Secondary Structure 
TTRYEQVQQDLKANPKTWLVTGVAGFIGSNLLETLLGLNQTVIGLDNFATGHQHNLDEV
CCHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCHHHH
QQTVTADQWANFTFIEGDIRSLDDCKKAVTGVDYVLHQAALGSVPRSIEDPITTNQANID
HHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCCCCCCCCHH
GFLNMLVAAKDENVASFTYAASSSTYGDHPGLPKVEDKIGKPLSPYAVTKYVNELYADVF
HHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHH
ARTYDFNTIGLRYFNVFGKRQDPNGAYAAVIPKWTAAMIQGEDVFINGDGETSRDFCFIE
HHHCCCCHHHHHHHHHHCCCCCCCCCEEEECCHHHHHEEECCEEEEECCCCCCCCHHHHH
NVVQINLLAATAEAEAKNQVFNVALGDRTTLNTLFDALKTALKECGVNYESDPVYRDFRA
HHEEEEEEEEHHHHHHHHCEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
GDVRHSQADISKAQELLGYEPAYRIYDGIGKAMPWYESTLNTGSDA
CCCCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NAD+ [C]

Metal ions: NA

Kcat value (1/min): 57600 [C]

Specific activity: 233.3

Km value (mM): 0.256 {UDPgalactose}} 0.225 {UDPgalactose}} 0.2 {UDPgalactose}} 0.18 {UDPgalactose}} 0.16 {UDPgalactose}} 0.14 {UDPgalactose}} 0.048 {UDPgalactose}} 0.026 {UDPgalactose}} [C]

Substrates: UDPglucose [C]

Specific reaction: UDPglucose <==> UDPglucoseal [C]

General reaction: Epimerization (specificity for carbon forming a hexosulose) [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8248629; 8331073; 11677608; 12644504 [H]