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The map label for this gene is phrB [H]

Identifier: 110833627

GI number: 110833627

Start: 859381

End: 860835

Strand: Direct

Name: phrB [H]

Synonym: ABO_0766

Alternate gene names: 110833627

Gene position: 859381-860835 (Clockwise)

Preceding gene: 110833626

Following gene: 110833628

Centisome position: 27.54

GC content: 58.01

Gene sequence:

>1455_bases
ATGAATCTGGTCTGGTACCGCAACGACCTGCGTGTCGCGGCACACTCGCCTCTGCACGCTGCGTTGACAGAGTCATCGAT
AAGCGCGGGTGAGACGAGGCCACAGGCGCTGGCGGTGTATTGTCTGTGTCAGGGGCAATGGGATCGTCATCAGGTGGCGC
CGTTGCGCCGCTGGTATGTGTTGGAAAGCTTGCGTGAGCTGGGCGAGTCCCTGGCTAACCGCGGCATTGATCTGCATGTG
CTGGATGTGGACACCTTCGAGAAGGTGCCGGAGGCGTTAGCAGAATTTGCCCAGAGCCAAGGTGTGACACACCTGTATTG
CAATCGTGAGTATCCGCTCAATGAAAAGCAGCGAGACACACTGGTGGCTGAACGCCTGCGTGAGCAAGGGATTATCCTGC
GCGGTTTCGACGATGGCGTGTTAGTTCCACCCGCGGCACTGCGCACCGGTAAAGGAACACCGTATACGGTGTTTGGGGCT
TATAAGAAACGTTGGGATGTATGGACGGCCGATCATCACCCGGCGATGACACCGGTGCCATCACAATGCAGGGCTGAAGG
TACGTTTATGGGGGCTAAGGTTGTGGCGAAGGCCCTTGAAATGCTGGACGTGCCTGAGGCGCTAACGCAGCAATGGGCGC
CTGGAGAAGAGGCCGCCTGGCAACAACTTGATCAATTTATGGAGCGGTCGCTGGCCGACTATCGCCGCAACCGTGATTTT
CCTGATTTGTCTGGCACCAGCGGCTTATCGGTGGCGCTATCGGCGGGAACCCTGTCGGTGGCCAGTTGTTTCCGGGCCGC
AACCCAGGCCATGGCGGATGCGGGTAGCCGTGATGGTGCTGCCTGCTGGATCGACGAGTTGGCCTGGCGGGATTTTTACC
GCCAGATCATGGCGCAGTTTCCTCGCGTTAGCCGCGGACAAGGCTTCCGGCCGGAAACGGATTTGCTGGAATGGAAAAAT
GATGACGAGCTATTCGCCGCTTGGTGTGAAGGGCGTACGGGGTACCCGTTGGTGGATGCAGCCATGCGGCAGCTGGTTGC
CACCGGCTGGATGCATAACCGGCTACGCATGGTGACAGCCATGTTTCTCAGTAAGCATCTTTGGCTGGATTGGCGCCGCG
GTGAAGCGTTCTTTATGACACATTTGATCGATGGGGACTTTGCCGCCAATAACGGTGGCTGGCAGTGGAGTGCCTCATCT
GGCACCGATGCGGTGCCTTATTTTCGGGTGTTTAATCCGGTTCGTCAGGGGCAACGTTTTGATAGCGAAGGCCGCTTTAT
TGCTCATTGGGTGCCGCAATTGCGCGACTTGGATAGCAAGCAAATCCATGAGCCCTGGAAGCAGCCGCTACTGGCCCGGG
ATTATCCACCACCGGTGGTGCCCCATGCGGGAGTGCGTGAGCGGGTGACGGTAGCGTTCAAGGCGGCTAAAAGCCGGTTT
GATAAAACCTCTTAG

Upstream 100 bases:

>100_bases
TATCGCAAATGACACTGCCGCAAGAGGTAGCCCAGCTTGGCGGGCGTCGGCAGGGGCTGGCGAGCGAACTGGATCGACTT
CAACAATCCTGGAGTACGCC

Downstream 100 bases:

>100_bases
GATCAGCAACACCCCGTGTCGCCGCCGGCCATGGGCAATAGTAGGAAGGGAAAGACATGAGTCAGCGTATTGCTATTGTT
GGAGCCGGTATTAGTGGGCT

Product: DNA photolyase

Products: NA

Alternate protein names: DNA photolyase; Photoreactivating enzyme [H]

Number of amino acids: Translated: 484; Mature: 484

Protein sequence:

>484_residues
MNLVWYRNDLRVAAHSPLHAALTESSISAGETRPQALAVYCLCQGQWDRHQVAPLRRWYVLESLRELGESLANRGIDLHV
LDVDTFEKVPEALAEFAQSQGVTHLYCNREYPLNEKQRDTLVAERLREQGIILRGFDDGVLVPPAALRTGKGTPYTVFGA
YKKRWDVWTADHHPAMTPVPSQCRAEGTFMGAKVVAKALEMLDVPEALTQQWAPGEEAAWQQLDQFMERSLADYRRNRDF
PDLSGTSGLSVALSAGTLSVASCFRAATQAMADAGSRDGAACWIDELAWRDFYRQIMAQFPRVSRGQGFRPETDLLEWKN
DDELFAAWCEGRTGYPLVDAAMRQLVATGWMHNRLRMVTAMFLSKHLWLDWRRGEAFFMTHLIDGDFAANNGGWQWSASS
GTDAVPYFRVFNPVRQGQRFDSEGRFIAHWVPQLRDLDSKQIHEPWKQPLLARDYPPPVVPHAGVRERVTVAFKAAKSRF
DKTS

Sequences:

>Translated_484_residues
MNLVWYRNDLRVAAHSPLHAALTESSISAGETRPQALAVYCLCQGQWDRHQVAPLRRWYVLESLRELGESLANRGIDLHV
LDVDTFEKVPEALAEFAQSQGVTHLYCNREYPLNEKQRDTLVAERLREQGIILRGFDDGVLVPPAALRTGKGTPYTVFGA
YKKRWDVWTADHHPAMTPVPSQCRAEGTFMGAKVVAKALEMLDVPEALTQQWAPGEEAAWQQLDQFMERSLADYRRNRDF
PDLSGTSGLSVALSAGTLSVASCFRAATQAMADAGSRDGAACWIDELAWRDFYRQIMAQFPRVSRGQGFRPETDLLEWKN
DDELFAAWCEGRTGYPLVDAAMRQLVATGWMHNRLRMVTAMFLSKHLWLDWRRGEAFFMTHLIDGDFAANNGGWQWSASS
GTDAVPYFRVFNPVRQGQRFDSEGRFIAHWVPQLRDLDSKQIHEPWKQPLLARDYPPPVVPHAGVRERVTVAFKAAKSRF
DKTS
>Mature_484_residues
MNLVWYRNDLRVAAHSPLHAALTESSISAGETRPQALAVYCLCQGQWDRHQVAPLRRWYVLESLRELGESLANRGIDLHV
LDVDTFEKVPEALAEFAQSQGVTHLYCNREYPLNEKQRDTLVAERLREQGIILRGFDDGVLVPPAALRTGKGTPYTVFGA
YKKRWDVWTADHHPAMTPVPSQCRAEGTFMGAKVVAKALEMLDVPEALTQQWAPGEEAAWQQLDQFMERSLADYRRNRDF
PDLSGTSGLSVALSAGTLSVASCFRAATQAMADAGSRDGAACWIDELAWRDFYRQIMAQFPRVSRGQGFRPETDLLEWKN
DDELFAAWCEGRTGYPLVDAAMRQLVATGWMHNRLRMVTAMFLSKHLWLDWRRGEAFFMTHLIDGDFAANNGGWQWSASS
GTDAVPYFRVFNPVRQGQRFDSEGRFIAHWVPQLRDLDSKQIHEPWKQPLLARDYPPPVVPHAGVRERVTVAFKAAKSRF
DKTS

Specific function: Involved in repair of UV radiation-induced DNA damage. Catalyzes the light-dependent monomerization (300-600 nm) of cyclobutyl pyrimidine dimers (in cis-syn configuration), which are formed between adjacent bases on the same DNA strand upon exposure to ul

COG id: COG0415

COG function: function code L; Deoxyribodipyrimidine photolyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DNA photolyase domain [H]

Homologues:

Organism=Homo sapiens, GI188536103, Length=429, Percent_Identity=29.3706293706294, Blast_Score=147, Evalue=2e-35,
Organism=Homo sapiens, GI188536100, Length=433, Percent_Identity=29.3302540415704, Blast_Score=145, Evalue=7e-35,
Organism=Homo sapiens, GI4758072, Length=436, Percent_Identity=26.3761467889908, Blast_Score=135, Evalue=8e-32,
Organism=Escherichia coli, GI1786926, Length=481, Percent_Identity=40.7484407484407, Blast_Score=366, Evalue=1e-102,
Organism=Saccharomyces cerevisiae, GI6324962, Length=497, Percent_Identity=31.7907444668008, Blast_Score=261, Evalue=2e-70,
Organism=Drosophila melanogaster, GI17137248, Length=503, Percent_Identity=26.6401590457256, Blast_Score=137, Evalue=1e-32,
Organism=Drosophila melanogaster, GI24585455, Length=503, Percent_Identity=26.6401590457256, Blast_Score=137, Evalue=1e-32,
Organism=Drosophila melanogaster, GI24648152, Length=529, Percent_Identity=23.6294896030246, Blast_Score=129, Evalue=4e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002081
- InterPro:   IPR018394
- InterPro:   IPR006050
- InterPro:   IPR005101
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00875 DNA_photolyase; PF03441 FAD_binding_7 [H]

EC number: =4.1.99.3 [H]

Molecular weight: Translated: 54843; Mature: 54843

Theoretical pI: Translated: 6.99; Mature: 6.99

Prosite motif: PS00394 DNA_PHOTOLYASES_1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLVWYRNDLRVAAHSPLHAALTESSISAGETRPQALAVYCLCQGQWDRHQVAPLRRWYV
CCEEEEECCCEEECCCCHHHHHHHCCCCCCCCCCCEEEEEEEECCCCCCHHHHHHHHHHH
LESLRELGESLANRGIDLHVLDVDTFEKVPEALAEFAQSQGVTHLYCNREYPLNEKQRDT
HHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCHHHHH
LVAERLREQGIILRGFDDGVLVPPAALRTGKGTPYTVFGAYKKRWDVWTADHHPAMTPVP
HHHHHHHHCCCEEEECCCCCCCCCHHHHCCCCCCEEEEECHHHHCCCEECCCCCCCCCCC
SQCRAEGTFMGAKVVAKALEMLDVPEALTQQWAPGEEAAWQQLDQFMERSLADYRRNRDF
HHHHCCCCHHHHHHHHHHHHHHCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCC
PDLSGTSGLSVALSAGTLSVASCFRAATQAMADAGSRDGAACWIDELAWRDFYRQIMAQF
CCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHC
PRVSRGQGFRPETDLLEWKNDDELFAAWCEGRTGYPLVDAAMRQLVATGWMHNRLRMVTA
CCCCCCCCCCCCCHHHCCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
MFLSKHLWLDWRRGEAFFMTHLIDGDFAANNGGWQWSASSGTDAVPYFRVFNPVRQGQRF
HHHHHHHEEEEECCCEEEEEEEECCCEECCCCCEEEECCCCCCCCCHHHHHHHHHHCCCC
DSEGRFIAHWVPQLRDLDSKQIHEPWKQPLLARDYPPPVVPHAGVRERVTVAFKAAKSRF
CCCCCCHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHC
DKTS
CCCC
>Mature Secondary Structure
MNLVWYRNDLRVAAHSPLHAALTESSISAGETRPQALAVYCLCQGQWDRHQVAPLRRWYV
CCEEEEECCCEEECCCCHHHHHHHCCCCCCCCCCCEEEEEEEECCCCCCHHHHHHHHHHH
LESLRELGESLANRGIDLHVLDVDTFEKVPEALAEFAQSQGVTHLYCNREYPLNEKQRDT
HHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCHHHHH
LVAERLREQGIILRGFDDGVLVPPAALRTGKGTPYTVFGAYKKRWDVWTADHHPAMTPVP
HHHHHHHHCCCEEEECCCCCCCCCHHHHCCCCCCEEEEECHHHHCCCEECCCCCCCCCCC
SQCRAEGTFMGAKVVAKALEMLDVPEALTQQWAPGEEAAWQQLDQFMERSLADYRRNRDF
HHHHCCCCHHHHHHHHHHHHHHCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCC
PDLSGTSGLSVALSAGTLSVASCFRAATQAMADAGSRDGAACWIDELAWRDFYRQIMAQF
CCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHC
PRVSRGQGFRPETDLLEWKNDDELFAAWCEGRTGYPLVDAAMRQLVATGWMHNRLRMVTA
CCCCCCCCCCCCCHHHCCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
MFLSKHLWLDWRRGEAFFMTHLIDGDFAANNGGWQWSASSGTDAVPYFRVFNPVRQGQRF
HHHHHHHEEEEECCCEEEEEEEECCCEECCCCCEEEECCCCCCCCCHHHHHHHHHHCCCC
DSEGRFIAHWVPQLRDLDSKQIHEPWKQPLLARDYPPPVVPHAGVRERVTVAFKAAKSRF
CCCCCCHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHC
DKTS
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6325460; 8905232; 9278503; 2200511; 7604260 [H]