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The map label for this gene is murE

Identifier: 110833454

GI number: 110833454

Start: 649580

End: 651052

Strand: Direct

Name: murE

Synonym: ABO_0593

Alternate gene names: 110833454

Gene position: 649580-651052 (Clockwise)

Preceding gene: 110833453

Following gene: 110833455

Centisome position: 20.82

GC content: 58.59

Gene sequence:

>1473_bases
ATGATGACTCTCCAGCAACTGCTGCCTAATACCACCCTGCCTGCTACTGCGGCGGGTGTTGTGCTTTCAGGGTTACAGCT
CGATAGCCGCAAAGTTATGCCCGGTGATGCTTTTTTGGCCGTGCAGGGCGTGGCCAGCGATGGTCGCCAGTTTGTCAGCC
AGGCGATTGCTGCCGGTGCGGTGGCAGTATTGGCTGAGGCAGAGACATTTTCTGTTCAGCAGGAGCAGGCGGTGCCACGG
GTGAATTTCCCCGGTCTGAGCCGTTCGGTGGGTGAGCTCGCGGCCCGTTGGTACGGGGAGCCTGGCTCGGCCATGAATGT
GGTGGGTGTGACGGGTACCAACGGCAAAACCAGCATTACTTGGTTCCTGCGTGATGCACTGAATGCGCTGGGCCATCGCT
GTGCGCTGGTCGGCACACTAGGCTTAGGCTTAAAAGATAATGAACAGATTACCGGCCATACCACTCCGGATCCGATTACC
CTGCAGGCCGGCTTAGCCCGAGTCCTTGAGGCGGGTGCCGATACAGTGGCTATGGAGGTGTCTTCCCATGCGCTGGATCA
GCATCGGCTGGGTCCGACTCCGGTGTCGGTGGCTGTATTCAGTAACTTGAGCCGCGATCATCTGGATTACCACGGCGACA
TGGAAGCCTATTTGGCAGCCAAATCTGTGTTATTCACCCGTGACGGCGTGAAGTTGGCGGTGATCAATTGTGACGACCCG
GTGGCGTATTCGTTGGTGGGTTGCCTGGCCGATGGCGTACGCTGTGTGACCTTCGGCGATCAGACCGGTGCCACCGTGCG
TTGTGCGTCTGTGGCGTTCACCGCCGAAGGCACCAATGCCGAACTGAACGTAGGCGGAGAGTCCTTCTCGGTCACTTTGC
CGCTGTTCGGCCGTTTCAACCTCAGCAACCTGATGGCCGTTGCCGGTATTCTGCATGGACAGGGTGTCAGCACGGATTCA
CTGGCTGGAGCTCTGGCTGCGATAACACCAGTGCCCGGTCGCATGGAACCGGTGCAGCAAGCTGGCTGTCCCACGGTGAT
TGTCGATTACGCCCACACTCCGGATGGGCTGGAAAAAGCTCTGCAAGCCTGTCGTGCGCACTTCGCTGGCCGCTTGTTCT
GCATCATTGGTTGCGGAGGCGACCGGGATACCGGCAAGCGCCCACAAATGGCCGCGGTCGCTGAGCGTGGCGCGGACCAC
GTGGTGTTTACCAGCGATAATCCGCGTAGCGAAGATCCGCAAAAAATCATTGATGAAATGTGCGCTGGTCTCACTGACAC
AGGGTCAGTGCAGATCATTGTTGAACGTGATCGAGCGGTGATGGATACGGTGCAGGCGGCCAGTGACGGCGATGTGATTC
TGCTTGCCGGCAAAGGCCATGAGGATTATCAGGAAGTGAATGGTGTGCGTTACCCCAGTGATGATCGCGATCTGGCCCAC
AAGGCGTTCAACGCTCGGGGAGGTGCACAATGA

Upstream 100 bases:

>100_bases
CATTTTCTCGCATTATGAGTGGCGTGTTGCGCTCGCTCCATGTGCCACCGGACAAGCCTGACGGGCTGCTGGCCGGCGGG
CCGGACGGGGAGGATCGCTC

Downstream 100 bases:

>100_bases
TGTGGCTGTCACAGATCGCCCAGTGGACCGGCGGTGCCCTGCACGGTGACGACTTGAAAGTGAATGCGGTCGCCACCGAT
ACCCGCGATATTGAACCCGG

Product: UDP-N-acetylmuramoylalanyl-D-glutamate--2, 6-diaminopimelate ligase

Products: NA

Alternate protein names: Meso-A2pm-adding enzyme; Meso-diaminopimelate-adding enzyme; UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligase; UDP-MurNAc-tripeptide synthetase; UDP-N-acetylmuramyl-tripeptide synthetase

Number of amino acids: Translated: 490; Mature: 490

Protein sequence:

>490_residues
MMTLQQLLPNTTLPATAAGVVLSGLQLDSRKVMPGDAFLAVQGVASDGRQFVSQAIAAGAVAVLAEAETFSVQQEQAVPR
VNFPGLSRSVGELAARWYGEPGSAMNVVGVTGTNGKTSITWFLRDALNALGHRCALVGTLGLGLKDNEQITGHTTPDPIT
LQAGLARVLEAGADTVAMEVSSHALDQHRLGPTPVSVAVFSNLSRDHLDYHGDMEAYLAAKSVLFTRDGVKLAVINCDDP
VAYSLVGCLADGVRCVTFGDQTGATVRCASVAFTAEGTNAELNVGGESFSVTLPLFGRFNLSNLMAVAGILHGQGVSTDS
LAGALAAITPVPGRMEPVQQAGCPTVIVDYAHTPDGLEKALQACRAHFAGRLFCIIGCGGDRDTGKRPQMAAVAERGADH
VVFTSDNPRSEDPQKIIDEMCAGLTDTGSVQIIVERDRAVMDTVQAASDGDVILLAGKGHEDYQEVNGVRYPSDDRDLAH
KAFNARGGAQ

Sequences:

>Translated_490_residues
MMTLQQLLPNTTLPATAAGVVLSGLQLDSRKVMPGDAFLAVQGVASDGRQFVSQAIAAGAVAVLAEAETFSVQQEQAVPR
VNFPGLSRSVGELAARWYGEPGSAMNVVGVTGTNGKTSITWFLRDALNALGHRCALVGTLGLGLKDNEQITGHTTPDPIT
LQAGLARVLEAGADTVAMEVSSHALDQHRLGPTPVSVAVFSNLSRDHLDYHGDMEAYLAAKSVLFTRDGVKLAVINCDDP
VAYSLVGCLADGVRCVTFGDQTGATVRCASVAFTAEGTNAELNVGGESFSVTLPLFGRFNLSNLMAVAGILHGQGVSTDS
LAGALAAITPVPGRMEPVQQAGCPTVIVDYAHTPDGLEKALQACRAHFAGRLFCIIGCGGDRDTGKRPQMAAVAERGADH
VVFTSDNPRSEDPQKIIDEMCAGLTDTGSVQIIVERDRAVMDTVQAASDGDVILLAGKGHEDYQEVNGVRYPSDDRDLAH
KAFNARGGAQ
>Mature_490_residues
MMTLQQLLPNTTLPATAAGVVLSGLQLDSRKVMPGDAFLAVQGVASDGRQFVSQAIAAGAVAVLAEAETFSVQQEQAVPR
VNFPGLSRSVGELAARWYGEPGSAMNVVGVTGTNGKTSITWFLRDALNALGHRCALVGTLGLGLKDNEQITGHTTPDPIT
LQAGLARVLEAGADTVAMEVSSHALDQHRLGPTPVSVAVFSNLSRDHLDYHGDMEAYLAAKSVLFTRDGVKLAVINCDDP
VAYSLVGCLADGVRCVTFGDQTGATVRCASVAFTAEGTNAELNVGGESFSVTLPLFGRFNLSNLMAVAGILHGQGVSTDS
LAGALAAITPVPGRMEPVQQAGCPTVIVDYAHTPDGLEKALQACRAHFAGRLFCIIGCGGDRDTGKRPQMAAVAERGADH
VVFTSDNPRSEDPQKIIDEMCAGLTDTGSVQIIVERDRAVMDTVQAASDGDVILLAGKGHEDYQEVNGVRYPSDDRDLAH
KAFNARGGAQ

Specific function: Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan

COG id: COG0769

COG function: function code M; UDP-N-acetylmuramyl tripeptide synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family. MurE subfamily

Homologues:

Organism=Escherichia coli, GI1786273, Length=464, Percent_Identity=43.1034482758621, Blast_Score=322, Evalue=5e-89,
Organism=Escherichia coli, GI1786274, Length=382, Percent_Identity=26.7015706806283, Blast_Score=67, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MURE_ALCBS (Q0VS07)

Other databases:

- EMBL:   AM286690
- RefSeq:   YP_692313.1
- ProteinModelPortal:   Q0VS07
- SMR:   Q0VS07
- STRING:   Q0VS07
- GeneID:   4211243
- GenomeReviews:   AM286690_GR
- KEGG:   abo:ABO_0593
- NMPDR:   fig|393595.12.peg.591
- eggNOG:   COG0769
- HOGENOM:   HBG602753
- OMA:   VLGCGGD
- PhylomeDB:   Q0VS07
- BioCyc:   ABOR393595:ABO_0593-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00208
- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005761
- Gene3D:   G3DSA:3.90.190.20
- Gene3D:   G3DSA:3.40.1190.10
- TIGRFAMs:   TIGR01085

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M; SSF53244 Mur_ligase_C; SSF53623 Mur_ligase_cen

EC number: =6.3.2.13

Molecular weight: Translated: 51222; Mature: 51222

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: NA

Important sites: BINDING 27-27 BINDING 29-29 BINDING 181-181 BINDING 187-187 BINDING 189-189 BINDING 382-382 BINDING 457-457 BINDING 461-461

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMTLQQLLPNTTLPATAAGVVLSGLQLDSRKVMPGDAFLAVQGVASDGRQFVSQAIAAGA
CCCHHHHCCCCCCCHHHHHHHHCCCEECCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHH
VAVLAEAETFSVQQEQAVPRVNFPGLSRSVGELAARWYGEPGSAMNVVGVTGTNGKTSIT
HHEEECCHHHCCHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCCEEEEEEECCCCCEEEE
WFLRDALNALGHRCALVGTLGLGLKDNEQITGHTTPDPITLQAGLARVLEAGADTVAMEV
HHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCCCCEEHHHHHHHHHHCCCCEEEEEH
SSHALDQHRLGPTPVSVAVFSNLSRDHLDYHGDMEAYLAAKSVLFTRDGVKLAVINCDDP
HHHHHHHHCCCCCCEEHHHHHCCCHHHCCCCCCHHHHHHHHHHEEEECCCEEEEEECCCH
VAYSLVGCLADGVRCVTFGDQTGATVRCASVAFTAEGTNAELNVGGESFSVTLPLFGRFN
HHHHHHHHHHCCEEEEEECCCCCCEEEEEEEEEEECCCCCEEEECCCEEEEEEEECCCCC
LSNLMAVAGILHGQGVSTDSLAGALAAITPVPGRMEPVQQAGCPTVIVDYAHTPDGLEKA
HHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCHHHHCCCCEEEEECCCCCHHHHHH
LQACRAHFAGRLFCIIGCGGDRDTGKRPQMAAVAERGADHVVFTSDNPRSEDPQKIIDEM
HHHHHHHCCCEEEEEEECCCCCCCCCCCCHHHHHHCCCCEEEECCCCCCCCCHHHHHHHH
CAGLTDTGSVQIIVERDRAVMDTVQAASDGDVILLAGKGHEDYQEVNGVRYPSDDRDLAH
HCCCCCCCCEEEEEECCHHHHHHHHCCCCCCEEEEECCCCHHHHHHCCCCCCCCCHHHHH
KAFNARGGAQ
HHHCCCCCCC
>Mature Secondary Structure
MMTLQQLLPNTTLPATAAGVVLSGLQLDSRKVMPGDAFLAVQGVASDGRQFVSQAIAAGA
CCCHHHHCCCCCCCHHHHHHHHCCCEECCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHH
VAVLAEAETFSVQQEQAVPRVNFPGLSRSVGELAARWYGEPGSAMNVVGVTGTNGKTSIT
HHEEECCHHHCCHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCCEEEEEEECCCCCEEEE
WFLRDALNALGHRCALVGTLGLGLKDNEQITGHTTPDPITLQAGLARVLEAGADTVAMEV
HHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCCCCEEHHHHHHHHHHCCCCEEEEEH
SSHALDQHRLGPTPVSVAVFSNLSRDHLDYHGDMEAYLAAKSVLFTRDGVKLAVINCDDP
HHHHHHHHCCCCCCEEHHHHHCCCHHHCCCCCCHHHHHHHHHHEEEECCCEEEEEECCCH
VAYSLVGCLADGVRCVTFGDQTGATVRCASVAFTAEGTNAELNVGGESFSVTLPLFGRFN
HHHHHHHHHHCCEEEEEECCCCCCEEEEEEEEEEECCCCCEEEECCCEEEEEEEECCCCC
LSNLMAVAGILHGQGVSTDSLAGALAAITPVPGRMEPVQQAGCPTVIVDYAHTPDGLEKA
HHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCHHHHCCCCEEEEECCCCCHHHHHH
LQACRAHFAGRLFCIIGCGGDRDTGKRPQMAAVAERGADHVVFTSDNPRSEDPQKIIDEM
HHHHHHHCCCEEEEEEECCCCCCCCCCCCHHHHHHCCCCEEEECCCCCCCCCHHHHHHHH
CAGLTDTGSVQIIVERDRAVMDTVQAASDGDVILLAGKGHEDYQEVNGVRYPSDDRDLAH
HCCCCCCCCEEEEEECCHHHHHHHHCCCCCCEEEEECCCCHHHHHHCCCCCCCCCHHHHH
KAFNARGGAQ
HHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA