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The map label for this gene is murF [H]

Identifier: 110833455

GI number: 110833455

Start: 651049

End: 652413

Strand: Direct

Name: murF [H]

Synonym: ABO_0594

Alternate gene names: 110833455

Gene position: 651049-652413 (Clockwise)

Preceding gene: 110833454

Following gene: 110833456

Centisome position: 20.87

GC content: 59.78

Gene sequence:

>1365_bases
ATGATGTGGCTGTCACAGATCGCCCAGTGGACCGGCGGTGCCCTGCACGGTGACGACTTGAAAGTGAATGCGGTCGCCAC
CGATACCCGCGATATTGAACCCGGCTGTCTGTTTGCCGCGCTGCGCGGTGAGCGCTTTGATGCCCATGATTTTCTAGCGT
CTGCCAAAAACGGTGGCGCCATTGCTGCTTTGGTCGAGCACGAGCAGGACTGTTTCGACAACGTTGTGCAGGTTGCCGAT
AGCCGTAAAGGTTTGGGGCGACTTGCCGGTGGCTATGCCGCTCAGTTCGGCATGCCCCGCCTGGCGGTGACCGGTAATGC
CGGCAAAACCACCGTGAAAGAAATGGCGGCGACCATGTTGGGCGAAGGTACCTTGGCCACCAATGGCAATTTTAATAATG
ACGTGGGTGTGCCGCTAACTCTGCTGCGGCTGCGTGCCGAGCATCGTTATGCCGTGATTGAGCTGGGTGCCAATGCACCG
GGCGAAATTGCGTGGACCAGTTCGTTGACGCGCCCGCAGGTGGTGTTAGTCACGAATGTAACCGGGGCGCATCTAGAGGG
CTTTGGCTCCATGCAGGGCATAGCCGACGCCAAGGCAGAAATTTTTACCGGTTGTACGCAGGGCGGTCAGGCGATCGTGA
ACAACGATGATAGCTTTGCCGATTTTTTTGCAGAAAAAGCCCGTGCTGCGGGGTTGACCGTGGTGCGGGTGAGTATGGAC
GAAAGCACACGGGCCGGAGCCGACCTGTATGCAGAGAACATTGTCCTCCACCGCGACAGTGCCGAGTTCGTACTTCAGCC
GCTGGGCGACGCTGTGCTTGTCCCGCTGCCGGGGGCGCATCAGGTGAGTAATGCCCTGATGGCCTGCGCAGCGGTCCGCG
CGCTAGGTGCTAGTTTGCCTCAGGTGTTGCCGCGACTGGCAGCGCTCAAGCCCGTGCCTGGGCGGATGCATATTCAATCC
GCCGGCACCGGCACGGTGGTTGACGATACTTATAACGCTAATCCGGGCTCGGTACGTGCAGCCATCGACTGGTTGGCCGC
ACAGCCCGCACCACAAATGCTGGTGCTGGGAGAAATGGGTGAGTTGGGCCCGCAGGCGGACAGCCTGGTAGAAGCATTAG
GTGAATACGCTCAGCAGGCCGGCATTGCCGGTTTGGTGGTGATGACCGGTGCAGCAGCAGCTGCACGGGGCTATGGCGAA
GCGGCAAACATTGCTAAGGACTATCAACAGGCGGCGAATTGGAGTGCCCCAGTGCTGGAACAAGGTGGCACTGTGTTGGT
GAAAGGGTCGCGCAGCGCCGGTATGGAAGCGGTGGTAAGGCTGTTGGCGACACAAGAACACACACTCACACAGGGGGCAC
GCTGA

Upstream 100 bases:

>100_bases
GCAAAGGCCATGAGGATTATCAGGAAGTGAATGGTGTGCGTTACCCCAGTGATGATCGCGATCTGGCCCACAAGGCGTTC
AACGCTCGGGGAGGTGCACA

Downstream 100 bases:

>100_bases
TGCTGCTTTGGCTGGCTGAATATCTTGCGCAATACCACAGCGGTTTTCTGGTGGTGCAGTACATCACTTTGCGCGGCATC
TTAAGTGTTCTCACTGCTTT

Product: UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alan ine ligase

Products: NA

Alternate protein names: D-alanyl-D-alanine-adding enzyme; UDP-MurNAc-pentapeptide synthetase [H]

Number of amino acids: Translated: 454; Mature: 454

Protein sequence:

>454_residues
MMWLSQIAQWTGGALHGDDLKVNAVATDTRDIEPGCLFAALRGERFDAHDFLASAKNGGAIAALVEHEQDCFDNVVQVAD
SRKGLGRLAGGYAAQFGMPRLAVTGNAGKTTVKEMAATMLGEGTLATNGNFNNDVGVPLTLLRLRAEHRYAVIELGANAP
GEIAWTSSLTRPQVVLVTNVTGAHLEGFGSMQGIADAKAEIFTGCTQGGQAIVNNDDSFADFFAEKARAAGLTVVRVSMD
ESTRAGADLYAENIVLHRDSAEFVLQPLGDAVLVPLPGAHQVSNALMACAAVRALGASLPQVLPRLAALKPVPGRMHIQS
AGTGTVVDDTYNANPGSVRAAIDWLAAQPAPQMLVLGEMGELGPQADSLVEALGEYAQQAGIAGLVVMTGAAAAARGYGE
AANIAKDYQQAANWSAPVLEQGGTVLVKGSRSAGMEAVVRLLATQEHTLTQGAR

Sequences:

>Translated_454_residues
MMWLSQIAQWTGGALHGDDLKVNAVATDTRDIEPGCLFAALRGERFDAHDFLASAKNGGAIAALVEHEQDCFDNVVQVAD
SRKGLGRLAGGYAAQFGMPRLAVTGNAGKTTVKEMAATMLGEGTLATNGNFNNDVGVPLTLLRLRAEHRYAVIELGANAP
GEIAWTSSLTRPQVVLVTNVTGAHLEGFGSMQGIADAKAEIFTGCTQGGQAIVNNDDSFADFFAEKARAAGLTVVRVSMD
ESTRAGADLYAENIVLHRDSAEFVLQPLGDAVLVPLPGAHQVSNALMACAAVRALGASLPQVLPRLAALKPVPGRMHIQS
AGTGTVVDDTYNANPGSVRAAIDWLAAQPAPQMLVLGEMGELGPQADSLVEALGEYAQQAGIAGLVVMTGAAAAARGYGE
AANIAKDYQQAANWSAPVLEQGGTVLVKGSRSAGMEAVVRLLATQEHTLTQGAR
>Mature_454_residues
MMWLSQIAQWTGGALHGDDLKVNAVATDTRDIEPGCLFAALRGERFDAHDFLASAKNGGAIAALVEHEQDCFDNVVQVAD
SRKGLGRLAGGYAAQFGMPRLAVTGNAGKTTVKEMAATMLGEGTLATNGNFNNDVGVPLTLLRLRAEHRYAVIELGANAP
GEIAWTSSLTRPQVVLVTNVTGAHLEGFGSMQGIADAKAEIFTGCTQGGQAIVNNDDSFADFFAEKARAAGLTVVRVSMD
ESTRAGADLYAENIVLHRDSAEFVLQPLGDAVLVPLPGAHQVSNALMACAAVRALGASLPQVLPRLAALKPVPGRMHIQS
AGTGTVVDDTYNANPGSVRAAIDWLAAQPAPQMLVLGEMGELGPQADSLVEALGEYAQQAGIAGLVVMTGAAAAARGYGE
AANIAKDYQQAANWSAPVLEQGGTVLVKGSRSAGMEAVVRLLATQEHTLTQGAR

Specific function: Involved in cell wall formation. Catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the precursor of murein [H]

COG id: COG0770

COG function: function code M; UDP-N-acetylmuramyl pentapeptide synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786274, Length=448, Percent_Identity=46.4285714285714, Blast_Score=310, Evalue=2e-85,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005863 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.10 [H]

Molecular weight: Translated: 47148; Mature: 47148

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMWLSQIAQWTGGALHGDDLKVNAVATDTRDIEPGCLFAALRGERFDAHDFLASAKNGGA
CCHHHHHHHHCCCCCCCCCEEEEEEECCCCCCCCCCEEEECCCCCCCHHHHHHHCCCCCE
IAALVEHEQDCFDNVVQVADSRKGLGRLAGGYAAQFGMPRLAVTGNAGKTTVKEMAATML
EEEEEHHHHHHHHHHHHHHHHHCCHHHHHCCHHHHCCCCEEEEECCCCHHHHHHHHHHHH
GEGTLATNGNFNNDVGVPLTLLRLRAEHRYAVIELGANAPGEIAWTSSLTRPQVVLVTNV
CCCEEEECCCCCCCCCCHHHHHHHHHCCCEEEEEECCCCCCCEEECCCCCCCEEEEEECC
TGAHLEGFGSMQGIADAKAEIFTGCTQGGQAIVNNDDSFADFFAEKARAAGLTVVRVSMD
CCCHHCCCCCCCCHHHHHHHHHHCCCCCCCEEECCCCHHHHHHHHHHHHCCEEEEEEECC
ESTRAGADLYAENIVLHRDSAEFVLQPLGDAVLVPLPGAHQVSNALMACAAVRALGASLP
CCCCCCCHHHHCCEEEECCCHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHCHHH
QVLPRLAALKPVPGRMHIQSAGTGTVVDDTYNANPGSVRAAIDWLAAQPAPQMLVLGEMG
HHHHHHHHCCCCCCCEEEECCCCCCEECCCCCCCCCHHHHHHHHHHCCCCCCEEEEECCC
ELGPQADSLVEALGEYAQQAGIAGLVVMTGAAAAARGYGEAANIAKDYQQAANWSAPVLE
CCCCCHHHHHHHHHHHHHHCCCCEEEEECCCHHHHCCCCHHHHHHHHHHHHCCCCCCHHH
QGGTVLVKGSRSAGMEAVVRLLATQEHTLTQGAR
CCCEEEEECCCCCCHHHHHHHHHCCHHHHCCCCC
>Mature Secondary Structure
MMWLSQIAQWTGGALHGDDLKVNAVATDTRDIEPGCLFAALRGERFDAHDFLASAKNGGA
CCHHHHHHHHCCCCCCCCCEEEEEEECCCCCCCCCCEEEECCCCCCCHHHHHHHCCCCCE
IAALVEHEQDCFDNVVQVADSRKGLGRLAGGYAAQFGMPRLAVTGNAGKTTVKEMAATML
EEEEEHHHHHHHHHHHHHHHHHCCHHHHHCCHHHHCCCCEEEEECCCCHHHHHHHHHHHH
GEGTLATNGNFNNDVGVPLTLLRLRAEHRYAVIELGANAPGEIAWTSSLTRPQVVLVTNV
CCCEEEECCCCCCCCCCHHHHHHHHHCCCEEEEEECCCCCCCEEECCCCCCCEEEEEECC
TGAHLEGFGSMQGIADAKAEIFTGCTQGGQAIVNNDDSFADFFAEKARAAGLTVVRVSMD
CCCHHCCCCCCCCHHHHHHHHHHCCCCCCCEEECCCCHHHHHHHHHHHHCCEEEEEEECC
ESTRAGADLYAENIVLHRDSAEFVLQPLGDAVLVPLPGAHQVSNALMACAAVRALGASLP
CCCCCCCHHHHCCEEEECCCHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHCHHH
QVLPRLAALKPVPGRMHIQSAGTGTVVDDTYNANPGSVRAAIDWLAAQPAPQMLVLGEMG
HHHHHHHHCCCCCCCEEEECCCCCCEECCCCCCCCCHHHHHHHHHHCCCCCCEEEEECCC
ELGPQADSLVEALGEYAQQAGIAGLVVMTGAAAAARGYGEAANIAKDYQQAANWSAPVLE
CCCCCHHHHHHHHHHHHHHCCCCEEEEECCCHHHHCCCCHHHHHHHHHHHHCCCCCCHHH
QGGTVLVKGSRSAGMEAVVRLLATQEHTLTQGAR
CCCEEEEECCCCCCHHHHHHHHHCCHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2668880; 8973200; 1630901; 9278503; 9166795; 2186811; 11090285 [H]