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The map label for this gene is prfA

Identifier: 110833384

GI number: 110833384

Start: 575060

End: 576145

Strand: Direct

Name: prfA

Synonym: ABO_0523

Alternate gene names: 110833384

Gene position: 575060-576145 (Clockwise)

Preceding gene: 110833383

Following gene: 110833385

Centisome position: 18.43

GC content: 55.62

Gene sequence:

>1086_bases
ATGAAAGCGTCATTGCAAGGCAAGCTCGATAAGCTTGCAGACCGGTTTGAAGAGTTGGCGGGGCTATTGTCCGATCCGGA
TGTGATTAGCAACCAGAACCAATTCCGTGACCTGTCCCGGGAATACGCTGAAATTGATCCAGTGGTGAAGTGTTATCGCC
AATACCAGCAGGCCGTGGAGGATCATGGTGCGGCTAAAGCGATGCAGGACGACAGCGACGCGGATATGCGTGAGATGGGC
GCGGAAGAAGCCCGTGATGCTCAGGAGCGCATGGAGGCGTTAGCGGCAGAGCTGCAAAAGCTGATGCTACCGAAGGATCC
CCGCGATGGAGCCAATGTGTTTCTTGAAGTTCGCGCCGGCACGGGGGGGGACGAAGCGGCCATTTTTGCCGGAGACCTAT
TCCGCATGTATTCCAAGTATGCGGATCAACGGGGTTGGAAAGTGGAGATGGTGAGCGCCAGTGAGGGCGAGCATGGCGGC
TATAAGGAAGTGATTGCACGGGTGATTGGCGATGGCGTCTATTCGCGGATGAAATTTGAATCCGGAGCCCACCGGGTGCA
ACGGGTGCCGGCCACAGAATCCCAGGGGCGTATTCATACCTCTGCCTGCACAGTGGCGATTATGGCTGAAGCGGAAGACC
TGGGGGATATCAAGATTCGCACTGAAGACCTGCGTATTGATACCTATCGTTCCTCCGGTGCTGGCGGTCAGCACGTTAAC
ACCACGGATTCGGCTGTGCGCATTACTCACTTACCCACCGGGGTGGTGGTGGAGTGCCAGGATGAGCGTAGCCAGCACAA
GAACAAGGCTCGGGCTATGAGCCTACTGAGCGCTAAGCTGTATGACGCGCAGCAGAATGCGGCCCATGCCGAGCAGGCTG
CGGAGCGTAAATCCCTCGTCGGGTCCGGCGATCGTTCAGAGCGTATCCGTACCTATAACTATCCTCAGGGACGAGTCACT
GATCATCGTATTAATCTTACCCTTTACCGGTTAAATGAAATCGTTGAAGGAGATTTGGATGAGATTTTGGGTGCGTTGCT
GGCAGAGTATCAGGCGGATCAGCTAGCGGCCTTAGGCGAGCATTAA

Upstream 100 bases:

>100_bases
CGACGACGACCAGTCGTAGCGCAAAGAACTCTCTCGATGTATCGAATTTTTGCGTGCGCCCGCCGGGCGGCGTATTTGCG
TGAAGCGTGGAAGCGAATTT

Downstream 100 bases:

>100_bases
CCGTGCGCATTGATGACGCCCTGCGCCAGGCTCGCCATCGGTTGGCGTCATCCCCTTGCGCAGCGCTGGATGCCCAAGTG
TTGCTTTGCCATGTGCTGGA

Product: peptide chain release factor 1

Products: NA

Alternate protein names: RF-1

Number of amino acids: Translated: 361; Mature: 361

Protein sequence:

>361_residues
MKASLQGKLDKLADRFEELAGLLSDPDVISNQNQFRDLSREYAEIDPVVKCYRQYQQAVEDHGAAKAMQDDSDADMREMG
AEEARDAQERMEALAAELQKLMLPKDPRDGANVFLEVRAGTGGDEAAIFAGDLFRMYSKYADQRGWKVEMVSASEGEHGG
YKEVIARVIGDGVYSRMKFESGAHRVQRVPATESQGRIHTSACTVAIMAEAEDLGDIKIRTEDLRIDTYRSSGAGGQHVN
TTDSAVRITHLPTGVVVECQDERSQHKNKARAMSLLSAKLYDAQQNAAHAEQAAERKSLVGSGDRSERIRTYNYPQGRVT
DHRINLTLYRLNEIVEGDLDEILGALLAEYQADQLAALGEH

Sequences:

>Translated_361_residues
MKASLQGKLDKLADRFEELAGLLSDPDVISNQNQFRDLSREYAEIDPVVKCYRQYQQAVEDHGAAKAMQDDSDADMREMG
AEEARDAQERMEALAAELQKLMLPKDPRDGANVFLEVRAGTGGDEAAIFAGDLFRMYSKYADQRGWKVEMVSASEGEHGG
YKEVIARVIGDGVYSRMKFESGAHRVQRVPATESQGRIHTSACTVAIMAEAEDLGDIKIRTEDLRIDTYRSSGAGGQHVN
TTDSAVRITHLPTGVVVECQDERSQHKNKARAMSLLSAKLYDAQQNAAHAEQAAERKSLVGSGDRSERIRTYNYPQGRVT
DHRINLTLYRLNEIVEGDLDEILGALLAEYQADQLAALGEH
>Mature_361_residues
MKASLQGKLDKLADRFEELAGLLSDPDVISNQNQFRDLSREYAEIDPVVKCYRQYQQAVEDHGAAKAMQDDSDADMREMG
AEEARDAQERMEALAAELQKLMLPKDPRDGANVFLEVRAGTGGDEAAIFAGDLFRMYSKYADQRGWKVEMVSASEGEHGG
YKEVIARVIGDGVYSRMKFESGAHRVQRVPATESQGRIHTSACTVAIMAEAEDLGDIKIRTEDLRIDTYRSSGAGGQHVN
TTDSAVRITHLPTGVVVECQDERSQHKNKARAMSLLSAKLYDAQQNAAHAEQAAERKSLVGSGDRSERIRTYNYPQGRVT
DHRINLTLYRLNEIVEGDLDEILGALLAEYQADQLAALGEH

Specific function: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA

COG id: COG0216

COG function: function code J; Protein chain release factor A

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family

Homologues:

Organism=Homo sapiens, GI166795303, Length=278, Percent_Identity=48.9208633093525, Blast_Score=265, Evalue=5e-71,
Organism=Homo sapiens, GI34577120, Length=366, Percent_Identity=38.2513661202186, Blast_Score=250, Evalue=1e-66,
Organism=Homo sapiens, GI166795305, Length=183, Percent_Identity=46.448087431694, Blast_Score=172, Evalue=4e-43,
Organism=Escherichia coli, GI1787462, Length=360, Percent_Identity=63.8888888888889, Blast_Score=463, Evalue=1e-132,
Organism=Escherichia coli, GI2367172, Length=350, Percent_Identity=38, Blast_Score=221, Evalue=7e-59,
Organism=Caenorhabditis elegans, GI17542784, Length=285, Percent_Identity=36.140350877193, Blast_Score=179, Evalue=2e-45,
Organism=Saccharomyces cerevisiae, GI6321295, Length=318, Percent_Identity=39.622641509434, Blast_Score=238, Evalue=1e-63,
Organism=Drosophila melanogaster, GI19921226, Length=321, Percent_Identity=40.1869158878505, Blast_Score=222, Evalue=3e-58,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): RF1_ALCBS (Q0VS77)

Other databases:

- EMBL:   AM286690
- RefSeq:   YP_692243.1
- ProteinModelPortal:   Q0VS77
- SMR:   Q0VS77
- STRING:   Q0VS77
- GeneID:   4212276
- GenomeReviews:   AM286690_GR
- KEGG:   abo:ABO_0523
- NMPDR:   fig|393595.12.peg.519
- eggNOG:   COG0216
- HOGENOM:   HBG629764
- OMA:   SEQGGYK
- PhylomeDB:   Q0VS77
- BioCyc:   ABOR393595:ABO_0523-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00093
- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR004373
- SMART:   SM00937
- TIGRFAMs:   TIGR00019

Pfam domain/function: PF03462 PCRF; PF00472 RF-1

EC number: NA

Molecular weight: Translated: 39994; Mature: 39994

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKASLQGKLDKLADRFEELAGLLSDPDVISNQNQFRDLSREYAEIDPVVKCYRQYQQAVE
CCCCHHHHHHHHHHHHHHHHHHCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DHGAAKAMQDDSDADMREMGAEEARDAQERMEALAAELQKLMLPKDPRDGANVFLEVRAG
HCCHHHHCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECC
TGGDEAAIFAGDLFRMYSKYADQRGWKVEMVSASEGEHGGYKEVIARVIGDGVYSRMKFE
CCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHH
SGAHRVQRVPATESQGRIHTSACTVAIMAEAEDLGDIKIRTEDLRIDTYRSSGAGGQHVN
HHHHHHHCCCCCCCCCCEEHHHEEEEEEECCCCCCCEEEEEECEEEEEECCCCCCCCCCC
TTDSAVRITHLPTGVVVECQDERSQHKNKARAMSLLSAKLYDAQQNAAHAEQAAERKSLV
CCCCEEEEEECCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GSGDRSERIRTYNYPQGRVTDHRINLTLYRLNEIVEGDLDEILGALLAEYQADQLAALGE
CCCCCCCCCEECCCCCCCCCCEEEEEEEEEHHHHHCCCHHHHHHHHHHHHHHHHHHHCCC
H
C
>Mature Secondary Structure
MKASLQGKLDKLADRFEELAGLLSDPDVISNQNQFRDLSREYAEIDPVVKCYRQYQQAVE
CCCCHHHHHHHHHHHHHHHHHHCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DHGAAKAMQDDSDADMREMGAEEARDAQERMEALAAELQKLMLPKDPRDGANVFLEVRAG
HCCHHHHCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECC
TGGDEAAIFAGDLFRMYSKYADQRGWKVEMVSASEGEHGGYKEVIARVIGDGVYSRMKFE
CCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHH
SGAHRVQRVPATESQGRIHTSACTVAIMAEAEDLGDIKIRTEDLRIDTYRSSGAGGQHVN
HHHHHHHCCCCCCCCCCEEHHHEEEEEEECCCCCCCEEEEEECEEEEEECCCCCCCCCCC
TTDSAVRITHLPTGVVVECQDERSQHKNKARAMSLLSAKLYDAQQNAAHAEQAAERKSLV
CCCCEEEEEECCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GSGDRSERIRTYNYPQGRVTDHRINLTLYRLNEIVEGDLDEILGALLAEYQADQLAALGE
CCCCCCCCCEECCCCCCCCCCEEEEEEEEEHHHHHCCCHHHHHHHHHHHHHHHHHHHCCC
H
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA