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The map label for this gene is dadA [C]

Identifier: 110833331

GI number: 110833331

Start: 510443

End: 511480

Strand: Direct

Name: dadA [C]

Synonym: ABO_0470

Alternate gene names: 110833331

Gene position: 510443-511480 (Clockwise)

Preceding gene: 110833329

Following gene: 110833335

Centisome position: 16.36

GC content: 54.91

Gene sequence:

>1038_bases
GTGACGATCATTGATGCCCCAGATAAATATCCGCCGGCCTCTTGGGCCGGTGGCGGCATTCTTTCCCCTTTATATGCTTG
GCGTTATTGCGATGCTATGACGCTCTTGTCGGTAAATGCGTTTTCCCGTTATCAGAAAGTTTTGGAAGAGTGTGATTCGG
CAGGTATACCTGGTTGTGGCTCACTGTTGCATGGCGGTGGTTTGTGGGTTGAAACGGCAGAAGAGGATGCTGCTCTAGCA
TTTGAATGGGCACAACGCTGGCATATTCCAGTACAAGCGGTCTTGGCCCATGAGCAAATGGAAGAATCTGCTGCTGGCGG
AGGGCTGTTGTTTCCGTCACTGGGTAATATTCGTAATCCGGGTGTACTAAGAGTGTTGCGTGCGTATTTGCTGCGGTTGG
GCGTGCGCTTTGAGTCGGCCATTATTCGCCATGTTCGGCCGTTGAATCTGGGTGGGGAAGTTGGGGCTGCTGATGGACGA
CGCTGGCACAGCGATGCCGTTGTGATCAGTGCTGGGCATGCAGCCCCAGGCCTTTTGCTCTCCATGGGGGTTACACTGCC
GCTTTTTCCTGCAAAAGGTGAGATGTTGCTGTATCACATGCAGCCCGGTCAGGTGCCTGCGGTTATGTTGACTGAGCGAG
GATATCTGATTCCGCGGCACGATGGCTCCGTGCTGGTGGGGTCAACGCTGAGGCGAGGCGATAATACGACATACCCTACC
GTAGCTGGGCGTTATCAGTTAGAGCGGTTGGCGGCGAGATTATGGCCGGAGCTGGCATCCTGTTCGCCAGCCTTTCATTG
GGCAGGGGTGCGACCGGGCTGTGAGCGTGATATTCCGTTTATTGGTTCGGTACCAGGAACCAATGGGGTGTTTGCGGCTG
TTGGCCATTATCGAAACGGGCTGGTGGCGGCGCCGGCCAGTGCGGAGTTGCTCGCCCAGTTGATTACTGGCGAGCAAACC
TTTGTTGATCCCGAGCCTTATTCGTTATCGTCTGTTTCGTCGTCATCACGGTCCAGCTCCAGCTTTTTCAATCGGTAG

Upstream 100 bases:

>100_bases
TCTGCTGTTTCCTCATATTGCCATGTCTGATGTGCTCGTTGTAGGTGGTGGAATTATCGGTCTTTTTTGTGCGCTTGAGC
TTACAAAGCGAGGCAGGAAA

Downstream 100 bases:

>100_bases
CGCAAAGAGCGGAAGGTCATGCCGAGTTTTTTTGCGGCGGCGGTACGATTCCAGTGAGTTGTATCCAGAGCCTTGAGTAT
TTCCTGTTTTTCGATTTCCT

Product: oxidoreductase

Products: FADH2; ammonia; Pyruvate [C]

Alternate protein names: NA

Number of amino acids: Translated: 345; Mature: 344

Protein sequence:

>345_residues
MTIIDAPDKYPPASWAGGGILSPLYAWRYCDAMTLLSVNAFSRYQKVLEECDSAGIPGCGSLLHGGGLWVETAEEDAALA
FEWAQRWHIPVQAVLAHEQMEESAAGGGLLFPSLGNIRNPGVLRVLRAYLLRLGVRFESAIIRHVRPLNLGGEVGAADGR
RWHSDAVVISAGHAAPGLLLSMGVTLPLFPAKGEMLLYHMQPGQVPAVMLTERGYLIPRHDGSVLVGSTLRRGDNTTYPT
VAGRYQLERLAARLWPELASCSPAFHWAGVRPGCERDIPFIGSVPGTNGVFAAVGHYRNGLVAAPASAELLAQLITGEQT
FVDPEPYSLSSVSSSSRSSSSFFNR

Sequences:

>Translated_345_residues
MTIIDAPDKYPPASWAGGGILSPLYAWRYCDAMTLLSVNAFSRYQKVLEECDSAGIPGCGSLLHGGGLWVETAEEDAALA
FEWAQRWHIPVQAVLAHEQMEESAAGGGLLFPSLGNIRNPGVLRVLRAYLLRLGVRFESAIIRHVRPLNLGGEVGAADGR
RWHSDAVVISAGHAAPGLLLSMGVTLPLFPAKGEMLLYHMQPGQVPAVMLTERGYLIPRHDGSVLVGSTLRRGDNTTYPT
VAGRYQLERLAARLWPELASCSPAFHWAGVRPGCERDIPFIGSVPGTNGVFAAVGHYRNGLVAAPASAELLAQLITGEQT
FVDPEPYSLSSVSSSSRSSSSFFNR
>Mature_344_residues
TIIDAPDKYPPASWAGGGILSPLYAWRYCDAMTLLSVNAFSRYQKVLEECDSAGIPGCGSLLHGGGLWVETAEEDAALAF
EWAQRWHIPVQAVLAHEQMEESAAGGGLLFPSLGNIRNPGVLRVLRAYLLRLGVRFESAIIRHVRPLNLGGEVGAADGRR
WHSDAVVISAGHAAPGLLLSMGVTLPLFPAKGEMLLYHMQPGQVPAVMLTERGYLIPRHDGSVLVGSTLRRGDNTTYPTV
AGRYQLERLAARLWPELASCSPAFHWAGVRPGCERDIPFIGSVPGTNGVFAAVGHYRNGLVAAPASAELLAQLITGEQTF
VDPEPYSLSSVSSSSRSSSSFFNR

Specific function: Oxidative Deamination Of D-Amino Acids. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Inner Membrane-Bound [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DadA oxidoreductase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006076
- InterPro:   IPR012727 [H]

Pfam domain/function: PF01266 DAO [H]

EC number: 1.4.99.1 [C]

Molecular weight: Translated: 37074; Mature: 36943

Theoretical pI: Translated: 6.85; Mature: 6.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIIDAPDKYPPASWAGGGILSPLYAWRYCDAMTLLSVNAFSRYQKVLEECDSAGIPGCG
CCEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCH
SLLHGGGLWVETAEEDAALAFEWAQRWHIPVQAVLAHEQMEESAAGGGLLFPSLGNIRNP
HHHCCCCEEEEECCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCEECCCCCCCCCC
GVLRVLRAYLLRLGVRFESAIIRHVRPLNLGGEVGAADGRRWHSDAVVISAGHAAPGLLL
HHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCEECCCEEEEECCCCCCCHHH
SMGVTLPLFPAKGEMLLYHMQPGQVPAVMLTERGYLIPRHDGSVLVGSTLRRGDNTTYPT
HHCCCEEEECCCCCEEEEEECCCCCCEEEEECCCEEEECCCCCEEECCHHHCCCCCCCCC
VAGRYQLERLAARLWPELASCSPAFHWAGVRPGCERDIPFIGSVPGTNGVFAAVGHYRNG
CCCHHHHHHHHHHHHHHHHCCCCCCEECCCCCCCCCCCCEEECCCCCCCCEEEHHHCCCC
LVAAPASAELLAQLITGEQTFVDPEPYSLSSVSSSSRSSSSFFNR
EEECCCHHHHHHHHHCCCCEECCCCCCCCHHCCCCCCCCHHHCCC
>Mature Secondary Structure 
TIIDAPDKYPPASWAGGGILSPLYAWRYCDAMTLLSVNAFSRYQKVLEECDSAGIPGCG
CEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCH
SLLHGGGLWVETAEEDAALAFEWAQRWHIPVQAVLAHEQMEESAAGGGLLFPSLGNIRNP
HHHCCCCEEEEECCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCEECCCCCCCCCC
GVLRVLRAYLLRLGVRFESAIIRHVRPLNLGGEVGAADGRRWHSDAVVISAGHAAPGLLL
HHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCEECCCEEEEECCCCCCCHHH
SMGVTLPLFPAKGEMLLYHMQPGQVPAVMLTERGYLIPRHDGSVLVGSTLRRGDNTTYPT
HHCCCEEEECCCCCEEEEEECCCCCCEEEEECCCEEEECCCCCEEECCHHHCCCCCCCCC
VAGRYQLERLAARLWPELASCSPAFHWAGVRPGCERDIPFIGSVPGTNGVFAAVGHYRNG
CCCHHHHHHHHHHHHHHHHCCCCCCEECCCCCCCCCCCCEEECCCCCCCCEEEHHHCCCC
LVAAPASAELLAQLITGEQTFVDPEPYSLSSVSSSSRSSSSFFNR
EEECCCHHHHHHHHHCCCCEECCCCCCCCHHCCCCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Iron [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 40 {D-proline}} 0.13 {K3Fe(CN)6}} 11 {D-methionine}} 0.63 {dichloroindophenol}} 0.01 {dichloroindophenol}} 19 {D-serine}} 12 {D-alpha-aminobutyrate}} 6 {D-phenylalanine}} 0.15 {cytochrome} 29 {D-alanine}} 6.4 {D-alanine}} 5.3 {D

Substrates: D-Alanine; FAD; H2O [C]

Specific reaction: D-Alanine + FAD + H2O --> FADH2 + ammonia + Pyruvate [C]

General reaction: Oxidative deamination; Redox reaction [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8682785; 10984043; 8097014 [H]