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The map label for this gene is nadE2 [H]

Identifier: 110833335

GI number: 110833335

Start: 514734

End: 516359

Strand: Direct

Name: nadE2 [H]

Synonym: ABO_0474

Alternate gene names: 110833335

Gene position: 514734-516359 (Clockwise)

Preceding gene: 110833331

Following gene: 110833337

Centisome position: 16.5

GC content: 56.64

Gene sequence:

>1626_bases
ATGCGAATTATTATGGCCCAGCAGAATGCGCTGGTGGGCGATATCGAAGGCAACGCCCAGCGCGTTATTGCCGCGTCAGA
CGAAGCTCGTCGATTATTAGGGGCAGATTTGGTGCTGTTCCCGGAACTAATGCTTACCGGCTACCCGCCAGAAGATCTTC
TGCTGCGCCCGAGTCTGAACAGTCGGGTCGATGCGGCCCTCGCAGATATCGGTGCGGCGATTTCGGTGCCCGTAGTGCTG
GGCTATCCGGCGGTACGCAACGGGCATCGTCGTAATGCGGCGGGGGTGATGTTCCCGGGTGAGACTGGTCCCCGTCATGA
GTATTTCAAGCAACACTTGCCCAATTACCGGGTGTTTGATGAAAAACGTTATTTTCAGCCGGGCAAGGATGCTTGCACCT
TTGATATTGATGGCTGGACGCTGGGTATTACCATTTGTGAAGACATTTGGCACGCTGGCCCAGCGGCCCAGTTACAGAAG
GCAGGCACCGATCTGATTCTGAATTTGAATGCCTCTCCTTATCGCATCAACAAGGCAGACGAACGTTTCGACCAGGTACA
AGCCCGTTGCCGGGAGACTGGCTTGCCGGTGTTGTATTGCAATCTTGTGGGTGGCCAGGATGAGCTGGTTTTTGATGGGG
CCTCATTTGTCTGCGATAGCCAGGGTAATCGCTGTGTGCAAGCTGCTAGTTTTACCGAAGCACTGGTGCCGGTGGATGTG
CAATGCCAGGGCGGGCACTGTGAGCCCCGTGGTGAGGTGTTGCCGCTGCCAGGGCCGCAGGCGGGTATGTACCAGGCTTT
GGTATTGGCAACCCGTGATTATGTGAACAAAAACGGTTTCAAGGGCGCTCTATTGGGACTGTCCGGGGGGATTGATTCTG
CTCTGACCCTGGCGGTGGCGGTGGATGCCCTTGGCCCGGAACGGGTGGAGGCGGTGATGATGCCATTTCAGTATACCTCT
GCCATGAGTCTGGAAGACGCAGAAAAACAGGCTCGAACCCTGCGCGTCCATTACCGGGTATTGCCGATCGAGGCAGCGTT
TAATGGGTTTATGGATATTCTCAGGGAGCCGTTTGCAGGCACCTCCCGGGACACAACCGAAGAAAACCTCCAGGCTCGCT
GTCGCGGGGTGCTGTTGATGGCGCTTTCCAACAAGAACGGTTCAGTCGTGTTGACCACGGGTAACAAGAGCGAAATGGCG
GTGGGGTATGCCACGCTGTATGGCGATATGGCGGGAGGCTTCTCGGTGCTCAAGGATGTCTTCAAGACGTCAGTGTTTAA
CCTGTGTCGTTGGCGCAACCAACAGGCTGGGGCGGAAATTATTCCCGACAGGGTGATTACGCGCCCTCCATCCGCAGAGT
TGGCTCCAGACCAAGTGGACAGTGATTCTCTGCCTGATTACGACGAGCTGGACGCGATTCTGGAGCGTTACGTGGAGCAG
GACATGAGCGCCGAAGCGGTGATTCGTGATGGTTTTGATCGAGAAACAGTGTATCGGGTGGTGAAACTCACGGACCGCAA
CGAGTATAAGCGTCGCCAGGCTGCCGTGGGGCCGCGCGTTAGCCGCCGGGCTTTCGGCAAGGACCGGCGCTATCCAATAA
CCAACGGTTGGCGGCCGGGAGATTAA

Upstream 100 bases:

>100_bases
GTTTTTGTCAGGTGCGGTCGGGTTACGTGTTTCTAGCGCATTCATCGATCGCCAGCTAAAGAGTACAATCAGCGCTCACT
TGAGGAAAGGCCGGTTGATC

Downstream 100 bases:

>100_bases
GTGGCTGTGGCGCAAGATGTTTGCTACTAGGTAAACACCCAAAAGCCCGCTATTGCGGGCTTTCTTCGTTAGGCGTCGGG
CGTTCTGTTTGCGTGCAGCA

Product: NAD+ synthase

Products: NA

Alternate protein names: NAD(+) synthase [glutamine-hydrolyzing] [H]

Number of amino acids: Translated: 541; Mature: 541

Protein sequence:

>541_residues
MRIIMAQQNALVGDIEGNAQRVIAASDEARRLLGADLVLFPELMLTGYPPEDLLLRPSLNSRVDAALADIGAAISVPVVL
GYPAVRNGHRRNAAGVMFPGETGPRHEYFKQHLPNYRVFDEKRYFQPGKDACTFDIDGWTLGITICEDIWHAGPAAQLQK
AGTDLILNLNASPYRINKADERFDQVQARCRETGLPVLYCNLVGGQDELVFDGASFVCDSQGNRCVQAASFTEALVPVDV
QCQGGHCEPRGEVLPLPGPQAGMYQALVLATRDYVNKNGFKGALLGLSGGIDSALTLAVAVDALGPERVEAVMMPFQYTS
AMSLEDAEKQARTLRVHYRVLPIEAAFNGFMDILREPFAGTSRDTTEENLQARCRGVLLMALSNKNGSVVLTTGNKSEMA
VGYATLYGDMAGGFSVLKDVFKTSVFNLCRWRNQQAGAEIIPDRVITRPPSAELAPDQVDSDSLPDYDELDAILERYVEQ
DMSAEAVIRDGFDRETVYRVVKLTDRNEYKRRQAAVGPRVSRRAFGKDRRYPITNGWRPGD

Sequences:

>Translated_541_residues
MRIIMAQQNALVGDIEGNAQRVIAASDEARRLLGADLVLFPELMLTGYPPEDLLLRPSLNSRVDAALADIGAAISVPVVL
GYPAVRNGHRRNAAGVMFPGETGPRHEYFKQHLPNYRVFDEKRYFQPGKDACTFDIDGWTLGITICEDIWHAGPAAQLQK
AGTDLILNLNASPYRINKADERFDQVQARCRETGLPVLYCNLVGGQDELVFDGASFVCDSQGNRCVQAASFTEALVPVDV
QCQGGHCEPRGEVLPLPGPQAGMYQALVLATRDYVNKNGFKGALLGLSGGIDSALTLAVAVDALGPERVEAVMMPFQYTS
AMSLEDAEKQARTLRVHYRVLPIEAAFNGFMDILREPFAGTSRDTTEENLQARCRGVLLMALSNKNGSVVLTTGNKSEMA
VGYATLYGDMAGGFSVLKDVFKTSVFNLCRWRNQQAGAEIIPDRVITRPPSAELAPDQVDSDSLPDYDELDAILERYVEQ
DMSAEAVIRDGFDRETVYRVVKLTDRNEYKRRQAAVGPRVSRRAFGKDRRYPITNGWRPGD
>Mature_541_residues
MRIIMAQQNALVGDIEGNAQRVIAASDEARRLLGADLVLFPELMLTGYPPEDLLLRPSLNSRVDAALADIGAAISVPVVL
GYPAVRNGHRRNAAGVMFPGETGPRHEYFKQHLPNYRVFDEKRYFQPGKDACTFDIDGWTLGITICEDIWHAGPAAQLQK
AGTDLILNLNASPYRINKADERFDQVQARCRETGLPVLYCNLVGGQDELVFDGASFVCDSQGNRCVQAASFTEALVPVDV
QCQGGHCEPRGEVLPLPGPQAGMYQALVLATRDYVNKNGFKGALLGLSGGIDSALTLAVAVDALGPERVEAVMMPFQYTS
AMSLEDAEKQARTLRVHYRVLPIEAAFNGFMDILREPFAGTSRDTTEENLQARCRGVLLMALSNKNGSVVLTTGNKSEMA
VGYATLYGDMAGGFSVLKDVFKTSVFNLCRWRNQQAGAEIIPDRVITRPPSAELAPDQVDSDSLPDYDELDAILERYVEQ
DMSAEAVIRDGFDRETVYRVVKLTDRNEYKRRQAAVGPRVSRRAFGKDRRYPITNGWRPGD

Specific function: Can use both glutamine or ammonia as a nitrogen source [H]

COG id: COG0171

COG function: function code H; NAD synthase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014445
- InterPro:   IPR022310
- InterPro:   IPR003694
- InterPro:   IPR000132
- InterPro:   IPR003010
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00795 CN_hydrolase; PF02540 NAD_synthase [H]

EC number: =6.3.5.1 [H]

Molecular weight: Translated: 59502; Mature: 59502

Theoretical pI: Translated: 5.09; Mature: 5.09

Prosite motif: PS00920 NITRIL_CHT_1 ; PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIIMAQQNALVGDIEGNAQRVIAASDEARRLLGADLVLFPELMLTGYPPEDLLLRPSLN
CEEEEECCCCEEEECCCCCEEEEEECHHHHHHHCCCHHHHHHHHHCCCCCHHCEECCCCC
SRVDAALADIGAAISVPVVLGYPAVRNGHRRNAAGVMFPGETGPRHEYFKQHLPNYRVFD
CHHHHHHHHHCCHHCCCEEECCHHHCCCCCCCCCEEEECCCCCCHHHHHHHHCCCCEEEC
EKRYFQPGKDACTFDIDGWTLGITICEDIWHAGPAAQLQKAGTDLILNLNASPYRINKAD
CHHCCCCCCCCEEEEECCEEEEHHHHHHHHCCCCHHHHHHCCCEEEEECCCCCEECCHHH
ERFDQVQARCRETGLPVLYCNLVGGQDELVFDGASFVCDSQGNRCVQAASFTEALVPVDV
HHHHHHHHHHHHCCCCEEEEEEECCCCCEEECCCCEEECCCCCCEEEHHHHHHHHCCEEE
QCQGGHCEPRGEVLPLPGPQAGMYQALVLATRDYVNKNGFKGALLGLSGGIDSALTLAVA
EECCCCCCCCCCEECCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHH
VDALGPERVEAVMMPFQYTSAMSLEDAEKQARTLRVHYRVLPIEAAFNGFMDILREPFAG
HHCCCHHHHHHEECCHHHCCCCCHHHHHHHHHEEEEEEEEEEHHHHHHHHHHHHHCCCCC
TSRDTTEENLQARCRGVLLMALSNKNGSVVLTTGNKSEMAVGYATLYGDMAGGFSVLKDV
CCCCCCHHHHHHHHCEEEEEEEECCCCEEEEEECCCCCEEEEHHHHHHCCCCHHHHHHHH
FKTSVFNLCRWRNQQAGAEIIPDRVITRPPSAELAPDQVDSDSLPDYDELDAILERYVEQ
HHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
DMSAEAVIRDGFDRETVYRVVKLTDRNEYKRRQAAVGPRVSRRAFGKDRRYPITNGWRPG
CCCHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHCCCCCCCCCCCCCCCC
D
C
>Mature Secondary Structure
MRIIMAQQNALVGDIEGNAQRVIAASDEARRLLGADLVLFPELMLTGYPPEDLLLRPSLN
CEEEEECCCCEEEECCCCCEEEEEECHHHHHHHCCCHHHHHHHHHCCCCCHHCEECCCCC
SRVDAALADIGAAISVPVVLGYPAVRNGHRRNAAGVMFPGETGPRHEYFKQHLPNYRVFD
CHHHHHHHHHCCHHCCCEEECCHHHCCCCCCCCCEEEECCCCCCHHHHHHHHCCCCEEEC
EKRYFQPGKDACTFDIDGWTLGITICEDIWHAGPAAQLQKAGTDLILNLNASPYRINKAD
CHHCCCCCCCCEEEEECCEEEEHHHHHHHHCCCCHHHHHHCCCEEEEECCCCCEECCHHH
ERFDQVQARCRETGLPVLYCNLVGGQDELVFDGASFVCDSQGNRCVQAASFTEALVPVDV
HHHHHHHHHHHHCCCCEEEEEEECCCCCEEECCCCEEECCCCCCEEEHHHHHHHHCCEEE
QCQGGHCEPRGEVLPLPGPQAGMYQALVLATRDYVNKNGFKGALLGLSGGIDSALTLAVA
EECCCCCCCCCCEECCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHH
VDALGPERVEAVMMPFQYTSAMSLEDAEKQARTLRVHYRVLPIEAAFNGFMDILREPFAG
HHCCCHHHHHHEECCHHHCCCCCHHHHHHHHHEEEEEEEEEEHHHHHHHHHHHHHCCCCC
TSRDTTEENLQARCRGVLLMALSNKNGSVVLTTGNKSEMAVGYATLYGDMAGGFSVLKDV
CCCCCCHHHHHHHHCEEEEEEEECCCCEEEEEECCCCCEEEEHHHHHHCCCCHHHHHHHH
FKTSVFNLCRWRNQQAGAEIIPDRVITRPPSAELAPDQVDSDSLPDYDELDAILERYVEQ
HHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
DMSAEAVIRDGFDRETVYRVVKLTDRNEYKRRQAAVGPRVSRRAFGKDRRYPITNGWRPG
CCCHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHCCCCCCCCCCCCCCCC
D
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10360571 [H]