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The map label for this gene is xylF [H]

Identifier: 110635443

GI number: 110635443

Start: 3381697

End: 3382740

Strand: Reverse

Name: xylF [H]

Synonym: Meso_3114

Alternate gene names: 110635443

Gene position: 3382740-3381697 (Counterclockwise)

Preceding gene: 110635445

Following gene: 110635442

Centisome position: 76.66

GC content: 59.2

Gene sequence:

>1044_bases
ATGAAAAATACCGCAACCGCCGTACTGGCGGGGATTGCCTTTTCTCTATCACTGTCGTCGGCGGCGCTTGCCGAAGGCAA
AACAATTGGGGTGTCCTGGTCAAATTTCCAGGAAGAGCGTTGGAAGACCGACGAGGCGGCCATCAAGGCGCAAATCGAGG
CCGATGGAAATACTTATATTTCCGCCGACGCACAGTCCTCGCCGGCCAAACAGCTTACAGACGTGGAATCCCTGATCGCG
CAGGGTGCCAACGCACTTATCATCCTCGCGCAGGATGCATCGGCGGTTGGCCCCGCCGTGGAAAATGCGGTCGCTGAAGG
AATTCCGGTGATCGGCTATGACCGCCTCATCGAAAATCCGGATGCGTTCTATCTGACCTTCGATAATAAAGAGGTCGGGC
GCATGCAGGCGCGCGCCGTGTTCGAGGTGGCGCCCGAGGGCAACTATGTCTTCATCAAGGGCTCTTCGGCCGATCCCAAC
GCCGATTTCCTGTTCGAGGGGCAGATGGAAGTCCTCAAGGAAGCCATCGATTCCGGCAAGATCAAGAATGTCGGCGAGGC
CTATACGGACGGCTGGCTGCCCGCCAATGCGCAACGCAACATGGAGCAGTTCCTGACCGCCAACAACAACGAGGTCGCTG
CAGTCGTTGCGTCGAATGACGGAACGGCGGGTGGTGCAATCGCTGCATTGGAAGCACAGGGTCTTGCGGGCTCGGTTCCC
GTTTCCGGGCAGGACGGTGATCATGCCGCGCTGAATCGCATTGCGCTCGGAACGCAGACCGTTTCCGTTTGGAAGGATGC
GCGTGAACTCGGTAAGGCCGCCGCGCAGATCGCCAATCAGCTTGCGGAGGGGACGGCGATGGATCAGGTTGAGGGCGCGA
CCAAGTGGGCCGAAGGCCCCAACAAAGTCGAGATGAACTCCGTCTTCCTGAAGCCGGTGCCGATCACGAAGGATAACCTC
AACCTCGTCATAGATGCCGGTTGGGTTACGAAGGATGTTGTCTGCCAGGGTGTGCAGCCCGGCACCGTCGACGTCTGCGG
CTGA

Upstream 100 bases:

>100_bases
TCTGGATAGACATCCCGTTTTGCACAAACGTGACATGTCAAATCCGCGGGATCGGCGCAAGTCAGAGGGAGGTCGCGCCA
CCGGCGCTGGGAGGAAAACT

Downstream 100 bases:

>100_bases
AAAGCGCTATAGCGGCGCAGAACGCCGCGGTTTGAGCGACCGCGGCGTTCTGCGTATCGGTCCCGGCCGTCTTGGCCGGA
CGGAGGAGACCAATATGACG

Product: D-xylose ABC transporter, periplasmic substrate-binding protein

Products: ADP; phosphate; xylose [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 347; Mature: 347

Protein sequence:

>347_residues
MKNTATAVLAGIAFSLSLSSAALAEGKTIGVSWSNFQEERWKTDEAAIKAQIEADGNTYISADAQSSPAKQLTDVESLIA
QGANALIILAQDASAVGPAVENAVAEGIPVIGYDRLIENPDAFYLTFDNKEVGRMQARAVFEVAPEGNYVFIKGSSADPN
ADFLFEGQMEVLKEAIDSGKIKNVGEAYTDGWLPANAQRNMEQFLTANNNEVAAVVASNDGTAGGAIAALEAQGLAGSVP
VSGQDGDHAALNRIALGTQTVSVWKDARELGKAAAQIANQLAEGTAMDQVEGATKWAEGPNKVEMNSVFLKPVPITKDNL
NLVIDAGWVTKDVVCQGVQPGTVDVCG

Sequences:

>Translated_347_residues
MKNTATAVLAGIAFSLSLSSAALAEGKTIGVSWSNFQEERWKTDEAAIKAQIEADGNTYISADAQSSPAKQLTDVESLIA
QGANALIILAQDASAVGPAVENAVAEGIPVIGYDRLIENPDAFYLTFDNKEVGRMQARAVFEVAPEGNYVFIKGSSADPN
ADFLFEGQMEVLKEAIDSGKIKNVGEAYTDGWLPANAQRNMEQFLTANNNEVAAVVASNDGTAGGAIAALEAQGLAGSVP
VSGQDGDHAALNRIALGTQTVSVWKDARELGKAAAQIANQLAEGTAMDQVEGATKWAEGPNKVEMNSVFLKPVPITKDNL
NLVIDAGWVTKDVVCQGVQPGTVDVCG
>Mature_347_residues
MKNTATAVLAGIAFSLSLSSAALAEGKTIGVSWSNFQEERWKTDEAAIKAQIEADGNTYISADAQSSPAKQLTDVESLIA
QGANALIILAQDASAVGPAVENAVAEGIPVIGYDRLIENPDAFYLTFDNKEVGRMQARAVFEVAPEGNYVFIKGSSADPN
ADFLFEGQMEVLKEAIDSGKIKNVGEAYTDGWLPANAQRNMEQFLTANNNEVAAVVASNDGTAGGAIAALEAQGLAGSVP
VSGQDGDHAALNRIALGTQTVSVWKDARELGKAAAQIANQLAEGTAMDQVEGATKWAEGPNKVEMNSVFLKPVPITKDNL
NLVIDAGWVTKDVVCQGVQPGTVDVCG

Specific function: Involved in the high-affinity D-xylose membrane transport system. Binds with high affinity to xylose [H]

COG id: COG4213

COG function: function code G; ABC-type xylose transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 2 family [H]

Homologues:

Organism=Escherichia coli, GI1789990, Length=310, Percent_Identity=42.258064516129, Blast_Score=232, Evalue=2e-62,
Organism=Escherichia coli, GI1788473, Length=338, Percent_Identity=28.1065088757396, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1790192, Length=257, Percent_Identity=31.5175097276265, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001761
- InterPro:   IPR013456 [H]

Pfam domain/function: PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 36272; Mature: 36272

Theoretical pI: Translated: 4.11; Mature: 4.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNTATAVLAGIAFSLSLSSAALAEGKTIGVSWSNFQEERWKTDEAAIKAQIEADGNTYI
CCCHHHHHHHHHHHHEECCHHHHCCCCEEEEEHHCCHHHHCCCCCCEEEEEEECCCCEEE
SADAQSSPAKQLTDVESLIAQGANALIILAQDASAVGPAVENAVAEGIPVIGYDRLIENP
ECCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHCCCCEEEHHHHHCCC
DAFYLTFDNKEVGRMQARAVFEVAPEGNYVFIKGSSADPNADFLFEGQMEVLKEAIDSGK
CEEEEEECCCHHHHHHEEEEEEECCCCCEEEEECCCCCCCCCEEECCHHHHHHHHHHCCC
IKNVGEAYTDGWLPANAQRNMEQFLTANNNEVAAVVASNDGTAGGAIAALEAQGLAGSVP
CCCCCHHHCCCCCCCCHHHHHHHHHCCCCCEEEEEEECCCCCCCCEEEEEEECCCCCCCC
VSGQDGDHAALNRIALGTQTVSVWKDARELGKAAAQIANQLAEGTAMDQVEGATKWAEGP
CCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCHHHCCCC
NKVEMNSVFLKPVPITKDNLNLVIDAGWVTKDVVCQGVQPGTVDVCG
CEEEECEEEEECCCCCCCCCEEEEECCCCCHHHHHCCCCCCEEEECC
>Mature Secondary Structure
MKNTATAVLAGIAFSLSLSSAALAEGKTIGVSWSNFQEERWKTDEAAIKAQIEADGNTYI
CCCHHHHHHHHHHHHEECCHHHHCCCCEEEEEHHCCHHHHCCCCCCEEEEEEECCCCEEE
SADAQSSPAKQLTDVESLIAQGANALIILAQDASAVGPAVENAVAEGIPVIGYDRLIENP
ECCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHCCCCEEEHHHHHCCC
DAFYLTFDNKEVGRMQARAVFEVAPEGNYVFIKGSSADPNADFLFEGQMEVLKEAIDSGK
CEEEEEECCCHHHHHHEEEEEEECCCCCEEEEECCCCCCCCCEEECCHHHHHHHHHHCCC
IKNVGEAYTDGWLPANAQRNMEQFLTANNNEVAAVVASNDGTAGGAIAALEAQGLAGSVP
CCCCCHHHCCCCCCCCHHHHHHHHHCCCCCEEEEEEECCCCCCCCEEEEEEECCCCCCCC
VSGQDGDHAALNRIALGTQTVSVWKDARELGKAAAQIANQLAEGTAMDQVEGATKWAEGP
CCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCHHHCCCC
NKVEMNSVFLKPVPITKDNLNLVIDAGWVTKDVVCQGVQPGTVDVCG
CEEEECEEEEECCCCCCCCCEEEEECCCCCHHHHHCCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; xylose [Periplasm]; H2O [C]

Specific reaction: ATP + xylose [Periplasm] + H2O = ADP + phosphate + xylose [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8041620; 9278503; 8581399; 9298646 [H]