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The map label for this gene is phoB [H]

Identifier: 110635445

GI number: 110635445

Start: 3384291

End: 3384980

Strand: Reverse

Name: phoB [H]

Synonym: Meso_3116

Alternate gene names: 110635445

Gene position: 3384980-3384291 (Counterclockwise)

Preceding gene: 110635446

Following gene: 110635443

Centisome position: 76.71

GC content: 62.03

Gene sequence:

>690_bases
ATGATCGCACCGAAAATCATGGTCGTGGAGGACGACGAGCCGCTTTGCGTGCTGCTTCGCTACAATCTTGAAGCCGAGGG
CTATCAGGTCGAAGTGGTCACGCGCGGTGACGAGGCTGAGGTGCGGCTGCAGGAAAACGTGCCGGACCTGCTCGTCCTCG
ACTGGATGGTACCCGCGATTTCAGGAATCGAGCTCTGCCGGCGCCTTAAAATGCGGCCTGAGACCGAGCGACTGCCCATC
ATCATGCTGACCGCCCGCGGCGAGGAGAGCGACCGCGTGCGCGGCCTTTCCACCGGCGCCGACGATTATCTGGTAAAGCC
CTTCTCCACGCCAGAATTCATCGCCCGCGTCCGGGCGCTTTTGCGGCGGGCAAAGCCGGAAGTGCTTTCAACGGTGCTCA
AGGTGGGCGATATCGTGCTCGACCGCGAAGCGCATCGCGTTTATCGGCGCAAGAACGAGATCCATCTTGGCCCCACGGAG
TTCCGCCTGCTCGAATTCATGATGCAGCATCCGGGCCGCGTCTTTTCGCGCAGCCAGCTCCTCGACAATGTCTGGGGCGA
GACGATCTATATCGACGAGCGCACGGTCGACGTCCATGTGGGCCGCCTGCGCAAGGCCGTTAACCAGGGCCGCATGCCGG
ATGTCATTCGCACCATCCGTGGGGCTGGATACGCTATCCGGGAGAACTGA

Upstream 100 bases:

>100_bases
AAAACGCCTATTATGTGCTCACCGGGCTCCAGCTTCCCGTTCAGCGGCCGAAGCAGGATGAAACCCAGATGACGGTCAAT
TCCTGAGCGGGGGCTTGTGA

Downstream 100 bases:

>100_bases
CGAGGGCCATCTTCCTGCGGCCCTTTGCCGGTTCATCCGAGCGAACCCCTGGGCCTCCGGCAAAAACGTGCCGGTCCAGG
TCCGTCAGCGCCGTCATCGC

Product: two component transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 229; Mature: 229

Protein sequence:

>229_residues
MIAPKIMVVEDDEPLCVLLRYNLEAEGYQVEVVTRGDEAEVRLQENVPDLLVLDWMVPAISGIELCRRLKMRPETERLPI
IMLTARGEESDRVRGLSTGADDYLVKPFSTPEFIARVRALLRRAKPEVLSTVLKVGDIVLDREAHRVYRRKNEIHLGPTE
FRLLEFMMQHPGRVFSRSQLLDNVWGETIYIDERTVDVHVGRLRKAVNQGRMPDVIRTIRGAGYAIREN

Sequences:

>Translated_229_residues
MIAPKIMVVEDDEPLCVLLRYNLEAEGYQVEVVTRGDEAEVRLQENVPDLLVLDWMVPAISGIELCRRLKMRPETERLPI
IMLTARGEESDRVRGLSTGADDYLVKPFSTPEFIARVRALLRRAKPEVLSTVLKVGDIVLDREAHRVYRRKNEIHLGPTE
FRLLEFMMQHPGRVFSRSQLLDNVWGETIYIDERTVDVHVGRLRKAVNQGRMPDVIRTIRGAGYAIREN
>Mature_229_residues
MIAPKIMVVEDDEPLCVLLRYNLEAEGYQVEVVTRGDEAEVRLQENVPDLLVLDWMVPAISGIELCRRLKMRPETERLPI
IMLTARGEESDRVRGLSTGADDYLVKPFSTPEFIARVRALLRRAKPEVLSTVLKVGDIVLDREAHRVYRRKNEIHLGPTE
FRLLEFMMQHPGRVFSRSQLLDNVWGETIYIDERTVDVHVGRLRKAVNQGRMPDVIRTIRGAGYAIREN

Specific function: This protein is a positive regulator for the phosphate regulon. Transcription of this operon is positively regulated by phoB and phoR when phosphate is limited [H]

COG id: COG0745

COG function: function code TK; Response regulators consisting of a CheY-like receiver domain and a winged-helix DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1786599, Length=223, Percent_Identity=47.5336322869955, Blast_Score=221, Evalue=4e-59,
Organism=Escherichia coli, GI1790860, Length=227, Percent_Identity=42.7312775330396, Blast_Score=162, Evalue=2e-41,
Organism=Escherichia coli, GI1786784, Length=222, Percent_Identity=36.9369369369369, Blast_Score=149, Evalue=1e-37,
Organism=Escherichia coli, GI1788394, Length=224, Percent_Identity=36.6071428571429, Blast_Score=148, Evalue=2e-37,
Organism=Escherichia coli, GI1789809, Length=229, Percent_Identity=37.117903930131, Blast_Score=141, Evalue=4e-35,
Organism=Escherichia coli, GI87082012, Length=223, Percent_Identity=36.322869955157, Blast_Score=134, Evalue=5e-33,
Organism=Escherichia coli, GI1786911, Length=226, Percent_Identity=36.283185840708, Blast_Score=133, Evalue=8e-33,
Organism=Escherichia coli, GI1790552, Length=225, Percent_Identity=36.4444444444444, Blast_Score=127, Evalue=8e-31,
Organism=Escherichia coli, GI2367329, Length=233, Percent_Identity=37.7682403433476, Blast_Score=125, Evalue=3e-30,
Organism=Escherichia coli, GI1787375, Length=220, Percent_Identity=33.6363636363636, Blast_Score=122, Evalue=2e-29,
Organism=Escherichia coli, GI1789402, Length=225, Percent_Identity=33.3333333333333, Blast_Score=121, Evalue=3e-29,
Organism=Escherichia coli, GI1790863, Length=241, Percent_Identity=32.3651452282158, Blast_Score=109, Evalue=1e-25,
Organism=Escherichia coli, GI1787229, Length=229, Percent_Identity=31.8777292576419, Blast_Score=98, Evalue=5e-22,
Organism=Escherichia coli, GI145693140, Length=233, Percent_Identity=31.3304721030043, Blast_Score=97, Evalue=8e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001867
- InterPro:   IPR016032
- InterPro:   IPR011879
- InterPro:   IPR001789
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00072 Response_reg; PF00486 Trans_reg_C [H]

EC number: NA

Molecular weight: Translated: 26358; Mature: 26358

Theoretical pI: Translated: 6.95; Mature: 6.95

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIAPKIMVVEDDEPLCVLLRYNLEAEGYQVEVVTRGDEAEVRLQENVPDLLVLDWMVPAI
CCCCEEEEEECCCCEEEEEEECCCCCCEEEEEEECCCCCEEEECCCCCCEEHHHHHHHHH
SGIELCRRLKMRPETERLPIIMLTARGEESDRVRGLSTGADDYLVKPFSTPEFIARVRAL
HHHHHHHHHCCCCCCCCCCEEEEEECCCCCHHEECCCCCCCCEEECCCCCHHHHHHHHHH
LRRAKPEVLSTVLKVGDIVLDREAHRVYRRKNEIHLGPTEFRLLEFMMQHPGRVFSRSQL
HHHCCHHHHHHHHHHHHHHCCHHHHHHHHHCCCEEECHHHHHHHHHHHHCCCHHHHHHHH
LDNVWGETIYIDERTVDVHVGRLRKAVNQGRMPDVIRTIRGAGYAIREN
HHHHCCCEEEEECCEEEHHHHHHHHHHHCCCCHHHHHHHHCCCEEEECC
>Mature Secondary Structure
MIAPKIMVVEDDEPLCVLLRYNLEAEGYQVEVVTRGDEAEVRLQENVPDLLVLDWMVPAI
CCCCEEEEEECCCCEEEEEEECCCCCCEEEEEEECCCCCEEEECCCCCCEEHHHHHHHHH
SGIELCRRLKMRPETERLPIIMLTARGEESDRVRGLSTGADDYLVKPFSTPEFIARVRAL
HHHHHHHHHCCCCCCCCCCEEEEEECCCCCHHEECCCCCCCCEEECCCCCHHHHHHHHHH
LRRAKPEVLSTVLKVGDIVLDREAHRVYRRKNEIHLGPTEFRLLEFMMQHPGRVFSRSQL
HHHCCHHHHHHHHHHHHHHCCHHHHHHHHHCCCEEECHHHHHHHHHHHHCCCHHHHHHHH
LDNVWGETIYIDERTVDVHVGRLRKAVNQGRMPDVIRTIRGAGYAIREN
HHHHCCCEEEEECCEEEHHHHHHHHHHHCCCCHHHHHHHHCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]