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The map label for this gene is ptsP [H]

Identifier: 110635329

GI number: 110635329

Start: 3267878

End: 3270148

Strand: Reverse

Name: ptsP [H]

Synonym: Meso_3000

Alternate gene names: 110635329

Gene position: 3270148-3267878 (Counterclockwise)

Preceding gene: 110635330

Following gene: 110635328

Centisome position: 74.11

GC content: 63.94

Gene sequence:

>2271_bases
ATGCGCGACATTGGCGGCGGCCCGCGTGTACTCTTAAAGCGGCTGCGTGAACTGATGGCAGAGCCGGTCGAGCCGCAGCA
GCGGCTCGACCGGATCGTGCGCGAGATCGCCCAGAACATGGTCGCGGAGGTCTGCTCGCTCTATGTGCTGCGCGCCGATT
CCGTGCTCGAGCTATATGCGACGGAGGGCCTCAATCCGACCGCGGTGCACCTGGCGCAGTTGCGCCTCGGGCAAGGCCTC
GTCGGCACGATCGCAGCGAGCGCGCGGCCGCTCAACCTTTCCGATGCGCAGAAACATCCGGCTTTTGCCTATCTGCCGGA
GACCGGAGAAGAGATCTACCATTCCTTTCTCGGCGTGCCGGTTTTAAGGGCGGGCCGTACGCTTGGCGTGCTGGTCGTCC
AGAACCGCACCATGCGGCACTACCGCGAGGACGAGGTGGAAGCGCTGGAAACGGTCGCCATGGTGGTGGCCGAGATGATC
GCCACGGGCGATCTTGCGCGTCTGACCCGGCCGGGCATCGAGCTCGATCTCAGGCGTGCTGCCAGCTTCAAGGGGCTGCC
GCTCAATGACGGCGTAGGGCTCGGCCATGTCGTGCTGCACGAGCCGCGCATCGTCGTCACCAATCTCTTCAACGAAGACA
GCGATCGCGAGCGGGAGCGCCTTGAGGAGGCGCTTTCCTCGCTCAGGCTTTCAATCGACGACATGCTTTCCCGGCGCGAT
GTGGCCTTCGAGGGCGAGCACCGGGCGGTGCTGGAAGCTTATCGCATGTTTGCCAATGACCGGGGCTGGGTGCGGCGGCT
GGAGGAGGCAGTCAGCAACGGGCTTACCGCCGAGGCCGCTGTCGAAAAAGTGCAGAGCGACATGCGCGCGCGGATGCTGC
ACATGACCGATCCTTATCTTCGCGAGCGCATGAGCGATTTCGACGATCTGGCAAACCGGCTTTTGCGCCAGCTCCTGGGC
CGTGGGCCGGAGGCGCTCGCCGACATCGTGCCGAAGGATGCGATCATCGTCGCCCGCTCCATGGGGGCGGCCGAACTGCT
CGACTATCCGCGCGAGCGGCTGCGGGGCCTGGTGCTGGAGGATGGCGCTCCGACCAGCCATATCGTGATCGTCGCGCGCG
CGATCGGCCTTCCGACGGTGGGGCAGGTGAAGGGCGCAGTCGCGATGTCGGAAAACGGCGATGCGATCGTTGTCGATGGT
GAGGACGGGCAGGTTCATCTGCGCCCGCAGGCCGATGTCGAAACGGCCTATGCGGAGAAGGTGCGTTTCCGCGCAAGACG
GCAGCAGCATTATCGGGAACTGCGCGACCGTCCTGCGGTCACCAAGGACGGTGTGCCGGTCCAGCTCCTGATGAATGCCG
GGCTGGCGGTCGATTTGCCCCAACTGGCGCAATCGGGAGCAGAAGGGATTGGCCTGTTTCGCACCGAGCTTCAATTCATG
GTGGTCTCCACCTTCCCGCGCGTCGAGGCGCAGGAGGGGCTTTACCGCGAGGTTCTGGACGCAGCGGGTGAAAAGCCGGT
TACGTTCCGCACCATCGATATCGGCGGCGACAAGGTGCTCCCCTACTTCAAGGGTGCGCAGCAGGAGGAAAATCCCGCCC
TGGGTTGGCGCGCGATCCGGTTGACGCTCGACAGGCCAGGGCTGTTACGCACGCAGATCCGCGCACTCCTGAAGGCGGCC
GGCGGGCGCGAGCTTAAGCTTATGCTGCCAATGGTGACGGAAATCGACGAGGTGCGGCAGGCTCGCGCCATCATCGACCG
GGAGGTCCGGCACTTATCGCGGTTCGCCCATCTTCTGCCGACCAGCTTGAAGCTTGGCGCGATGATCGAGGTGCCGGCGC
TCCTCTGGCAACTCGAAGAGCTGATGCAAGCGGTGGATTTCGTGTCGGTAGGATCGAACGATCTTTTTCAGTTCATCACC
GCCGCGGACCGGGGGAATACGCTGATTTCCAACCGTTTCGATCCGCTTGGCGTGCCGTTCCTCAGGGTGCTGAAGCAGAT
CGCGGCGGCAGGGCAGGCGACGCACACGCCGGTGACACTCTGCGGAGAGCTCGCCGGCAAACCAATCTCCGCCATGGCGC
TGCTCGGCATCGGCTATCGGTCCATTTCGATGAATTCCGCTTCCATCGGCCCGGTGAAGGCCATGCTTTGCGAACTGCCG
CTGCAGAAGCTCAGCGAACGGCTTGAAGCAGCCCTTGCCAATCGCAGCGGGAAGATCGATATCCGGGCCGAGCTGCAGCA
GTTTGCCAGCGAATACGACATTCCTCTTTAG

Upstream 100 bases:

>100_bases
TTCCGTCTACGGTCTGGATAAGCATTAGGACAGTTTTCTTTAACCATGCTTTGCGCGCTGCCAGATGGGCCAGCGCCCGC
GAAGCTTTGGAGGAAGTCCG

Downstream 100 bases:

>100_bases
ATAGAACAAGGGAAATATGGCCGAGCTGCCGCGCGAACGGATGGACCAGGTTTTGAAGCGTTTCGAGCTGATCGAAACGC
AGATGGCGGCCGGCCCTTCG

Product: phosphoenolpyruvate-protein phosphotransferase PtsP

Products: NA

Alternate protein names: Enzyme I-Ntr; Phosphotransferase system, enzyme I [H]

Number of amino acids: Translated: 756; Mature: 756

Protein sequence:

>756_residues
MRDIGGGPRVLLKRLRELMAEPVEPQQRLDRIVREIAQNMVAEVCSLYVLRADSVLELYATEGLNPTAVHLAQLRLGQGL
VGTIAASARPLNLSDAQKHPAFAYLPETGEEIYHSFLGVPVLRAGRTLGVLVVQNRTMRHYREDEVEALETVAMVVAEMI
ATGDLARLTRPGIELDLRRAASFKGLPLNDGVGLGHVVLHEPRIVVTNLFNEDSDRERERLEEALSSLRLSIDDMLSRRD
VAFEGEHRAVLEAYRMFANDRGWVRRLEEAVSNGLTAEAAVEKVQSDMRARMLHMTDPYLRERMSDFDDLANRLLRQLLG
RGPEALADIVPKDAIIVARSMGAAELLDYPRERLRGLVLEDGAPTSHIVIVARAIGLPTVGQVKGAVAMSENGDAIVVDG
EDGQVHLRPQADVETAYAEKVRFRARRQQHYRELRDRPAVTKDGVPVQLLMNAGLAVDLPQLAQSGAEGIGLFRTELQFM
VVSTFPRVEAQEGLYREVLDAAGEKPVTFRTIDIGGDKVLPYFKGAQQEENPALGWRAIRLTLDRPGLLRTQIRALLKAA
GGRELKLMLPMVTEIDEVRQARAIIDREVRHLSRFAHLLPTSLKLGAMIEVPALLWQLEELMQAVDFVSVGSNDLFQFIT
AADRGNTLISNRFDPLGVPFLRVLKQIAAAGQATHTPVTLCGELAGKPISAMALLGIGYRSISMNSASIGPVKAMLCELP
LQKLSERLEAALANRSGKIDIRAELQQFASEYDIPL

Sequences:

>Translated_756_residues
MRDIGGGPRVLLKRLRELMAEPVEPQQRLDRIVREIAQNMVAEVCSLYVLRADSVLELYATEGLNPTAVHLAQLRLGQGL
VGTIAASARPLNLSDAQKHPAFAYLPETGEEIYHSFLGVPVLRAGRTLGVLVVQNRTMRHYREDEVEALETVAMVVAEMI
ATGDLARLTRPGIELDLRRAASFKGLPLNDGVGLGHVVLHEPRIVVTNLFNEDSDRERERLEEALSSLRLSIDDMLSRRD
VAFEGEHRAVLEAYRMFANDRGWVRRLEEAVSNGLTAEAAVEKVQSDMRARMLHMTDPYLRERMSDFDDLANRLLRQLLG
RGPEALADIVPKDAIIVARSMGAAELLDYPRERLRGLVLEDGAPTSHIVIVARAIGLPTVGQVKGAVAMSENGDAIVVDG
EDGQVHLRPQADVETAYAEKVRFRARRQQHYRELRDRPAVTKDGVPVQLLMNAGLAVDLPQLAQSGAEGIGLFRTELQFM
VVSTFPRVEAQEGLYREVLDAAGEKPVTFRTIDIGGDKVLPYFKGAQQEENPALGWRAIRLTLDRPGLLRTQIRALLKAA
GGRELKLMLPMVTEIDEVRQARAIIDREVRHLSRFAHLLPTSLKLGAMIEVPALLWQLEELMQAVDFVSVGSNDLFQFIT
AADRGNTLISNRFDPLGVPFLRVLKQIAAAGQATHTPVTLCGELAGKPISAMALLGIGYRSISMNSASIGPVKAMLCELP
LQKLSERLEAALANRSGKIDIRAELQQFASEYDIPL
>Mature_756_residues
MRDIGGGPRVLLKRLRELMAEPVEPQQRLDRIVREIAQNMVAEVCSLYVLRADSVLELYATEGLNPTAVHLAQLRLGQGL
VGTIAASARPLNLSDAQKHPAFAYLPETGEEIYHSFLGVPVLRAGRTLGVLVVQNRTMRHYREDEVEALETVAMVVAEMI
ATGDLARLTRPGIELDLRRAASFKGLPLNDGVGLGHVVLHEPRIVVTNLFNEDSDRERERLEEALSSLRLSIDDMLSRRD
VAFEGEHRAVLEAYRMFANDRGWVRRLEEAVSNGLTAEAAVEKVQSDMRARMLHMTDPYLRERMSDFDDLANRLLRQLLG
RGPEALADIVPKDAIIVARSMGAAELLDYPRERLRGLVLEDGAPTSHIVIVARAIGLPTVGQVKGAVAMSENGDAIVVDG
EDGQVHLRPQADVETAYAEKVRFRARRQQHYRELRDRPAVTKDGVPVQLLMNAGLAVDLPQLAQSGAEGIGLFRTELQFM
VVSTFPRVEAQEGLYREVLDAAGEKPVTFRTIDIGGDKVLPYFKGAQQEENPALGWRAIRLTLDRPGLLRTQIRALLKAA
GGRELKLMLPMVTEIDEVRQARAIIDREVRHLSRFAHLLPTSLKLGAMIEVPALLWQLEELMQAVDFVSVGSNDLFQFIT
AADRGNTLISNRFDPLGVPFLRVLKQIAAAGQATHTPVTLCGELAGKPISAMALLGIGYRSISMNSASIGPVKAMLCELP
LQKLSERLEAALANRSGKIDIRAELQQFASEYDIPL

Specific function: Component of the phosphoenolpyruvate-dependent nitrogen- metabolic phosphotransferase system (nitrogen-metabolic PTS), that seems to be involved in regulating nitrogen metabolism. Enzyme I- Ntr transfers the phosphoryl group from phosphoenolpyruvate (PEP)

COG id: COG3605

COG function: function code T; Signal transduction protein containing GAF and PtsI domains

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GAF domain [H]

Homologues:

Organism=Escherichia coli, GI1789193, Length=738, Percent_Identity=35.9078590785908, Blast_Score=392, Evalue=1e-110,
Organism=Escherichia coli, GI1788756, Length=558, Percent_Identity=32.4372759856631, Blast_Score=281, Evalue=1e-76,
Organism=Escherichia coli, GI1788726, Length=573, Percent_Identity=32.8097731239092, Blast_Score=268, Evalue=1e-72,
Organism=Escherichia coli, GI48994992, Length=544, Percent_Identity=29.9632352941176, Blast_Score=242, Evalue=5e-65,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003018
- InterPro:   IPR008279
- InterPro:   IPR006318
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR008731
- InterPro:   IPR015813 [H]

Pfam domain/function: PF01590 GAF; PF05524 PEP-utilisers_N; PF00391 PEP-utilizers; PF02896 PEP-utilizers_C [H]

EC number: =2.7.3.9 [H]

Molecular weight: Translated: 83512; Mature: 83512

Theoretical pI: Translated: 6.20; Mature: 6.20

Prosite motif: PS00228 TUBULIN_B_AUTOREG

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRDIGGGPRVLLKRLRELMAEPVEPQQRLDRIVREIAQNMVAEVCSLYVLRADSVLELYA
CCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
TEGLNPTAVHLAQLRLGQGLVGTIAASARPLNLSDAQKHPAFAYLPETGEEIYHSFLGVP
HCCCCHHHHHHHHHHHCCCHHHHHHCCCCCCCCCCCCCCCCEEECCCCHHHHHHHHHCCH
VLRAGRTLGVLVVQNRTMRHYREDEVEALETVAMVVAEMIATGDLARLTRPGIELDLRRA
HHHCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCEEHHHH
ASFKGLPLNDGVGLGHVVLHEPRIVVTNLFNEDSDRERERLEEALSSLRLSIDDMLSRRD
CCCCCCCCCCCCCCCEEEEECCEEEEEECCCCCCHHHHHHHHHHHHHHHHCHHHHHHHCC
VAFEGEHRAVLEAYRMFANDRGWVRRLEEAVSNGLTAEAAVEKVQSDMRARMLHMTDPYL
CCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCHHH
RERMSDFDDLANRLLRQLLGRGPEALADIVPKDAIIVARSMGAAELLDYPRERLRGLVLE
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHCCEEEE
DGAPTSHIVIVARAIGLPTVGQVKGAVAMSENGDAIVVDGEDGQVHLRPQADVETAYAEK
CCCCCCCEEEEEHHHCCCCCHHCCCCEEECCCCCEEEEECCCCEEEECCCCCHHHHHHHH
VRFRARRQQHYRELRDRPAVTKDGVPVQLLMNAGLAVDLPQLAQSGAEGIGLFRTELQFM
HHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHCCCCCEECHHHHHCCCCCCHHHHHHHHHH
VVSTFPRVEAQEGLYREVLDAAGEKPVTFRTIDIGGDKVLPYFKGAQQEENPALGWRAIR
HHHCCCCCHHHHHHHHHHHHHCCCCCEEEEEEECCCCEECCHHCCCCCCCCCCCCEEEEE
LTLDRPGLLRTQIRALLKAAGGRELKLMLPMVTEIDEVRQARAIIDREVRHLSRFAHLLP
EEECCCCHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
TSLKLGAMIEVPALLWQLEELMQAVDFVSVGSNDLFQFITAADRGNTLISNRFDPLGVPF
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHCCCCCCCCHH
LRVLKQIAAAGQATHTPVTLCGELAGKPISAMALLGIGYRSISMNSASIGPVKAMLCELP
HHHHHHHHHCCCCCCCHHHHHHHHCCCCHHHHHHHHCCCCEEECCCCCCCHHHHHHHHHH
LQKLSERLEAALANRSGKIDIRAELQQFASEYDIPL
HHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MRDIGGGPRVLLKRLRELMAEPVEPQQRLDRIVREIAQNMVAEVCSLYVLRADSVLELYA
CCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
TEGLNPTAVHLAQLRLGQGLVGTIAASARPLNLSDAQKHPAFAYLPETGEEIYHSFLGVP
HCCCCHHHHHHHHHHHCCCHHHHHHCCCCCCCCCCCCCCCCEEECCCCHHHHHHHHHCCH
VLRAGRTLGVLVVQNRTMRHYREDEVEALETVAMVVAEMIATGDLARLTRPGIELDLRRA
HHHCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCEEHHHH
ASFKGLPLNDGVGLGHVVLHEPRIVVTNLFNEDSDRERERLEEALSSLRLSIDDMLSRRD
CCCCCCCCCCCCCCCEEEEECCEEEEEECCCCCCHHHHHHHHHHHHHHHHCHHHHHHHCC
VAFEGEHRAVLEAYRMFANDRGWVRRLEEAVSNGLTAEAAVEKVQSDMRARMLHMTDPYL
CCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCHHH
RERMSDFDDLANRLLRQLLGRGPEALADIVPKDAIIVARSMGAAELLDYPRERLRGLVLE
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHCCEEEE
DGAPTSHIVIVARAIGLPTVGQVKGAVAMSENGDAIVVDGEDGQVHLRPQADVETAYAEK
CCCCCCCEEEEEHHHCCCCCHHCCCCEEECCCCCEEEEECCCCEEEECCCCCHHHHHHHH
VRFRARRQQHYRELRDRPAVTKDGVPVQLLMNAGLAVDLPQLAQSGAEGIGLFRTELQFM
HHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHCCCCCEECHHHHHCCCCCCHHHHHHHHHH
VVSTFPRVEAQEGLYREVLDAAGEKPVTFRTIDIGGDKVLPYFKGAQQEENPALGWRAIR
HHHCCCCCHHHHHHHHHHHHHCCCCCEEEEEEECCCCEECCHHCCCCCCCCCCCCEEEEE
LTLDRPGLLRTQIRALLKAAGGRELKLMLPMVTEIDEVRQARAIIDREVRHLSRFAHLLP
EEECCCCHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
TSLKLGAMIEVPALLWQLEELMQAVDFVSVGSNDLFQFITAADRGNTLISNRFDPLGVPF
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHCCCCCCCCHH
LRVLKQIAAAGQATHTPVTLCGELAGKPISAMALLGIGYRSISMNSASIGPVKAMLCELP
HHHHHHHHHCCCCCCCHHHHHHHHCCCCHHHHHHHHCCCCEEECCCCCCCHHHHHHHHHH
LQKLSERLEAALANRSGKIDIRAELQQFASEYDIPL
HHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9278503; 7896715; 8973315 [H]