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The map label for this gene is prfA

Identifier: 110635328

GI number: 110635328

Start: 3266782

End: 3267861

Strand: Reverse

Name: prfA

Synonym: Meso_2999

Alternate gene names: 110635328

Gene position: 3267861-3266782 (Counterclockwise)

Preceding gene: 110635329

Following gene: 110635327

Centisome position: 74.06

GC content: 62.69

Gene sequence:

>1080_bases
ATGGCCGAGCTGCCGCGCGAACGGATGGACCAGGTTTTGAAGCGTTTCGAGCTGATCGAAACGCAGATGGCGGCCGGCCC
TTCGCCGGATGCTTATGTGAAGCTCGCCTCCGAATATTCAGAGCTGCAGGAGGTGGCGAACAGTATCCGCGCGCTTCGGA
AGGCGGAGGCGGAACTGGAAGACCTCGATACGATGCTGGCGGACAAGAGCATCGACCGCGAGATACGCGAGCTTGCGGAA
ATCGACCGGGAAGAGGTGGAAAGCCGCGTTGAAGACATGCAGCAGAAACTTCAAGTTCTGCTGCTCCCGAAAGACGAGGC
GGATGAGAAAAACGCCATCCTGGAGATTCGCGCCGGGACGGGCGGCGACGAGGCGGCACTGTTTGCCGGCGATCTTTTCC
GGATGTACGAACGCTACGCTAGCGAGCGTGGCTGGCGCGTTGAGGTGGTTTCGGCCAGCGAAGGCGAGGCCGGTGGCTAC
AAGGAGATTATCGCTTCGGTAACAGGGCGCGGGGTCTTTTCACGGCTAAAATTCGAGTCGGGCGTGCATCGCGTGCAGCG
CGTTCCGGCCACGGAAGCGCAGGGGCGCATCCACACCTCCGCCGCCACAGTGGCGGTGCTGCCCGAGGCGGAAGACATCG
ACGTGGATATCAAACCCGAGGATATCCGCATCGATACGATGCGGGCTTCAGGCGCAGGCGGCCAGCACGTGAACACGACC
GACTCGGCCGTGCGCATCACGCATTTGCCGACCGGCATCATGGTCGTTTCCGCGGAAAAGTCGCAGCATCAGAACCGGGC
GCGGGCGATGCAGATCCTGCGCGCGCGCCTGTTCGACATGGAGCGCTCGCGCGCGGCCAACGAGCGCTCGGAGGCGCGAC
GGCTGCAGGTTGGCACCGGCGACCGTTCGGAGCGCATCCGCACCTACAATTTCCCCCAGGGGCGTGTGACAGATCATCGA
ATCAATCTCACACTCTACAAGCTCGACCGTGTGATGGAGGGCGAGCTCGATGAAGTCATCGATGCGCTGATCGCCGATCA
CCAGTCGAAACTGCTGGCGGCGGAGGGAGCGGAGAGCTGA

Upstream 100 bases:

>100_bases
GCAGCCCTTGCCAATCGCAGCGGGAAGATCGATATCCGGGCCGAGCTGCAGCAGTTTGCCAGCGAATACGACATTCCTCT
TTAGATAGAACAAGGGAAAT

Downstream 100 bases:

>100_bases
TATGCCCCCGATCACGCTCGCTGCTCTGTTGCGAACTGCCCGGCAGCAACTGACGAGCGCCGGTGTGGAGGACGCGGCCT
TGGAGGCGCGGCTGATCGTC

Product: peptide chain release factor 1

Products: NA

Alternate protein names: RF-1

Number of amino acids: Translated: 359; Mature: 358

Protein sequence:

>359_residues
MAELPRERMDQVLKRFELIETQMAAGPSPDAYVKLASEYSELQEVANSIRALRKAEAELEDLDTMLADKSIDREIRELAE
IDREEVESRVEDMQQKLQVLLLPKDEADEKNAILEIRAGTGGDEAALFAGDLFRMYERYASERGWRVEVVSASEGEAGGY
KEIIASVTGRGVFSRLKFESGVHRVQRVPATEAQGRIHTSAATVAVLPEAEDIDVDIKPEDIRIDTMRASGAGGQHVNTT
DSAVRITHLPTGIMVVSAEKSQHQNRARAMQILRARLFDMERSRAANERSEARRLQVGTGDRSERIRTYNFPQGRVTDHR
INLTLYKLDRVMEGELDEVIDALIADHQSKLLAAEGAES

Sequences:

>Translated_359_residues
MAELPRERMDQVLKRFELIETQMAAGPSPDAYVKLASEYSELQEVANSIRALRKAEAELEDLDTMLADKSIDREIRELAE
IDREEVESRVEDMQQKLQVLLLPKDEADEKNAILEIRAGTGGDEAALFAGDLFRMYERYASERGWRVEVVSASEGEAGGY
KEIIASVTGRGVFSRLKFESGVHRVQRVPATEAQGRIHTSAATVAVLPEAEDIDVDIKPEDIRIDTMRASGAGGQHVNTT
DSAVRITHLPTGIMVVSAEKSQHQNRARAMQILRARLFDMERSRAANERSEARRLQVGTGDRSERIRTYNFPQGRVTDHR
INLTLYKLDRVMEGELDEVIDALIADHQSKLLAAEGAES
>Mature_358_residues
AELPRERMDQVLKRFELIETQMAAGPSPDAYVKLASEYSELQEVANSIRALRKAEAELEDLDTMLADKSIDREIRELAEI
DREEVESRVEDMQQKLQVLLLPKDEADEKNAILEIRAGTGGDEAALFAGDLFRMYERYASERGWRVEVVSASEGEAGGYK
EIIASVTGRGVFSRLKFESGVHRVQRVPATEAQGRIHTSAATVAVLPEAEDIDVDIKPEDIRIDTMRASGAGGQHVNTTD
SAVRITHLPTGIMVVSAEKSQHQNRARAMQILRARLFDMERSRAANERSEARRLQVGTGDRSERIRTYNFPQGRVTDHRI
NLTLYKLDRVMEGELDEVIDALIADHQSKLLAAEGAES

Specific function: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA

COG id: COG0216

COG function: function code J; Protein chain release factor A

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family

Homologues:

Organism=Homo sapiens, GI166795303, Length=311, Percent_Identity=47.588424437299, Blast_Score=294, Evalue=7e-80,
Organism=Homo sapiens, GI34577120, Length=347, Percent_Identity=37.1757925072046, Blast_Score=244, Evalue=1e-64,
Organism=Homo sapiens, GI166795305, Length=208, Percent_Identity=47.5961538461538, Blast_Score=203, Evalue=2e-52,
Organism=Escherichia coli, GI1787462, Length=325, Percent_Identity=60, Blast_Score=375, Evalue=1e-105,
Organism=Escherichia coli, GI2367172, Length=345, Percent_Identity=35.0724637681159, Blast_Score=191, Evalue=6e-50,
Organism=Caenorhabditis elegans, GI17542784, Length=271, Percent_Identity=39.8523985239852, Blast_Score=196, Evalue=1e-50,
Organism=Saccharomyces cerevisiae, GI6321295, Length=316, Percent_Identity=45.253164556962, Blast_Score=254, Evalue=2e-68,
Organism=Drosophila melanogaster, GI19921226, Length=332, Percent_Identity=42.4698795180723, Blast_Score=227, Evalue=9e-60,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): RF1_MESSB (Q11E04)

Other databases:

- EMBL:   CP000390
- RefSeq:   YP_675536.1
- ProteinModelPortal:   Q11E04
- SMR:   Q11E04
- STRING:   Q11E04
- GeneID:   4182414
- GenomeReviews:   CP000390_GR
- KEGG:   mes:Meso_2999
- NMPDR:   fig|266779.1.peg.4303
- eggNOG:   COG0216
- HOGENOM:   HBG629764
- OMA:   GEAGGYK
- PhylomeDB:   Q11E04
- ProtClustDB:   PRK00591
- BioCyc:   MSP266779:MESO_2999-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00093
- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR004373
- SMART:   SM00937
- TIGRFAMs:   TIGR00019

Pfam domain/function: PF03462 PCRF; PF00472 RF-1

EC number: NA

Molecular weight: Translated: 40144; Mature: 40012

Theoretical pI: Translated: 4.78; Mature: 4.78

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAELPRERMDQVLKRFELIETQMAAGPSPDAYVKLASEYSELQEVANSIRALRKAEAELE
CCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DLDTMLADKSIDREIRELAEIDREEVESRVEDMQQKLQVLLLPKDEADEKNAILEIRAGT
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHEEECCCCCCCCCCCEEEEECCC
GGDEAALFAGDLFRMYERYASERGWRVEVVSASEGEAGGYKEIIASVTGRGVFSRLKFES
CCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHCCHHHHHHHHHH
GVHRVQRVPATEAQGRIHTSAATVAVLPEAEDIDVDIKPEDIRIDTMRASGAGGQHVNTT
HHHHHHHCCCCCCCCEEEECCEEEEECCCCCCCCEECCCCCEEEEEEEECCCCCCCCCCC
DSAVRITHLPTGIMVVSAEKSQHQNRARAMQILRARLFDMERSRAANERSEARRLQVGTG
CCEEEEEECCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCC
DRSERIRTYNFPQGRVTDHRINLTLYKLDRVMEGELDEVIDALIADHQSKLLAAEGAES
CCCCCCEEECCCCCCCCCEEEEEEHEEHHHHHCCCHHHHHHHHHHHHHHHEECCCCCCC
>Mature Secondary Structure 
AELPRERMDQVLKRFELIETQMAAGPSPDAYVKLASEYSELQEVANSIRALRKAEAELE
CCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DLDTMLADKSIDREIRELAEIDREEVESRVEDMQQKLQVLLLPKDEADEKNAILEIRAGT
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHEEECCCCCCCCCCCEEEEECCC
GGDEAALFAGDLFRMYERYASERGWRVEVVSASEGEAGGYKEIIASVTGRGVFSRLKFES
CCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHCCHHHHHHHHHH
GVHRVQRVPATEAQGRIHTSAATVAVLPEAEDIDVDIKPEDIRIDTMRASGAGGQHVNTT
HHHHHHHCCCCCCCCEEEECCEEEEECCCCCCCCEECCCCCEEEEEEEECCCCCCCCCCC
DSAVRITHLPTGIMVVSAEKSQHQNRARAMQILRARLFDMERSRAANERSEARRLQVGTG
CCEEEEEECCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCC
DRSERIRTYNFPQGRVTDHRINLTLYKLDRVMEGELDEVIDALIADHQSKLLAAEGAES
CCCCCCEEECCCCCCCCCEEEEEEHEEHHHHHCCCHHHHHHHHHHHHHHHEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA