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The map label for this gene is hemK [H]

Identifier: 110635327

GI number: 110635327

Start: 3265914

End: 3266780

Strand: Reverse

Name: hemK [H]

Synonym: Meso_2998

Alternate gene names: 110635327

Gene position: 3266780-3265914 (Counterclockwise)

Preceding gene: 110635328

Following gene: 110635326

Centisome position: 74.04

GC content: 61.71

Gene sequence:

>867_bases
ATGCCCCCGATCACGCTCGCTGCTCTGTTGCGAACTGCCCGGCAGCAACTGACGAGCGCCGGTGTGGAGGACGCGGCCTT
GGAGGCGCGGCTGATCGTCGAGCATTTTTCCGAAACGACACAGACCCAGGCCATCACGCATCCCGAGTGCGCGGTGGAAG
CCGCCGTGGCGGAAAAGGTGGCGCGGGCGGTGGAACGGCGGATCGCGGGCGAGCCTGTCCACCGCATCCTCGGCCATCGT
GACTTTTATGGATTGAAGCTTGCGCTTTCGCCAGAAACGCTGGAGCCACGGCCGGATACGGAGACGCTGGTCGACCTCGT
ACTGCCGGAAGCGCGACGGATCGCAAACGTCAAAAAGCATTGCCGCATTCTCGATCTTGGGACTGGAACGGGCGCGATTG
CGCTTGCTCTTCTAAGCGTTGTGCCTGAAGCCGAAGCCGTGGGAACCGATATTTCGGAAGGGGCTCTTGAAACGGCGCGT
CACAATGCCGACATGAGTTTTGTTGCAGAGCGGTTCACCCCTCTGCGGTCGGATTGGTTTCGGAATGTGGATGGCCGATT
CGACCTGATTGTTTCCAACCCGCCCTATATCGCAACGGCGGAAATGGAAACGCTGCCGCGCGAAGTGCGCGAGCATGATC
CGAAGGCGGCACTCGATGGCGGTCCGGATGGGCTCATGCCCTATAGAGCGATCGCCAGCGGAGCGCGGGATCACTTGACG
AGGGAGGGTCTTATCGCCGTGGAAACAGGTGCCGAACAGAAAGCTGCCGTGGCGGCCATTTTCGCTGCCGAAGGCTATGC
GGTTTCAAGAACCGCACGCGATCTGGGCGGCCGAGACCGCGCGATGCTGTTTGCCCCAACCGCCTAA

Upstream 100 bases:

>100_bases
CTCGACCGTGTGATGGAGGGCGAGCTCGATGAAGTCATCGATGCGCTGATCGCCGATCACCAGTCGAAACTGCTGGCGGC
GGAGGGAGCGGAGAGCTGAT

Downstream 100 bases:

>100_bases
ATGTGCGATGCGGAAAAAACACTTGGCAAAGAGTTGGATTGCCGCTAGGTTCCGCCCTGCCGATGAGACAAAGCAGGCAG
CGCCGTTTTCGATTCGGTTC

Product: HemK family modification methylase

Products: NA

Alternate protein names: M.MloHemKP [H]

Number of amino acids: Translated: 288; Mature: 287

Protein sequence:

>288_residues
MPPITLAALLRTARQQLTSAGVEDAALEARLIVEHFSETTQTQAITHPECAVEAAVAEKVARAVERRIAGEPVHRILGHR
DFYGLKLALSPETLEPRPDTETLVDLVLPEARRIANVKKHCRILDLGTGTGAIALALLSVVPEAEAVGTDISEGALETAR
HNADMSFVAERFTPLRSDWFRNVDGRFDLIVSNPPYIATAEMETLPREVREHDPKAALDGGPDGLMPYRAIASGARDHLT
REGLIAVETGAEQKAAVAAIFAAEGYAVSRTARDLGGRDRAMLFAPTA

Sequences:

>Translated_288_residues
MPPITLAALLRTARQQLTSAGVEDAALEARLIVEHFSETTQTQAITHPECAVEAAVAEKVARAVERRIAGEPVHRILGHR
DFYGLKLALSPETLEPRPDTETLVDLVLPEARRIANVKKHCRILDLGTGTGAIALALLSVVPEAEAVGTDISEGALETAR
HNADMSFVAERFTPLRSDWFRNVDGRFDLIVSNPPYIATAEMETLPREVREHDPKAALDGGPDGLMPYRAIASGARDHLT
REGLIAVETGAEQKAAVAAIFAAEGYAVSRTARDLGGRDRAMLFAPTA
>Mature_287_residues
PPITLAALLRTARQQLTSAGVEDAALEARLIVEHFSETTQTQAITHPECAVEAAVAEKVARAVERRIAGEPVHRILGHRD
FYGLKLALSPETLEPRPDTETLVDLVLPEARRIANVKKHCRILDLGTGTGAIALALLSVVPEAEAVGTDISEGALETARH
NADMSFVAERFTPLRSDWFRNVDGRFDLIVSNPPYIATAEMETLPREVREHDPKAALDGGPDGLMPYRAIASGARDHLTR
EGLIAVETGAEQKAAVAAIFAAEGYAVSRTARDLGGRDRAMLFAPTA

Specific function: Probable protein methyltransferase. May methylate a Gln residue in target proteins [H]

COG id: COG2890

COG function: function code J; Methylase of polypeptide chain release factors

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hemK family [H]

Homologues:

Organism=Homo sapiens, GI7705409, Length=277, Percent_Identity=31.7689530685921, Blast_Score=99, Evalue=6e-21,
Organism=Escherichia coli, GI1787463, Length=278, Percent_Identity=35.9712230215827, Blast_Score=138, Evalue=5e-34,
Organism=Escherichia coli, GI87082085, Length=196, Percent_Identity=35.7142857142857, Blast_Score=104, Evalue=9e-24,
Organism=Drosophila melanogaster, GI24582226, Length=266, Percent_Identity=30.8270676691729, Blast_Score=120, Evalue=8e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR004556
- InterPro:   IPR019874
- InterPro:   IPR007848 [H]

Pfam domain/function: PF05175 MTS [H]

EC number: 2.1.1.-

Molecular weight: Translated: 31038; Mature: 30906

Theoretical pI: Translated: 5.25; Mature: 5.25

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPPITLAALLRTARQQLTSAGVEDAALEARLIVEHFSETTQTQAITHPECAVEAAVAEKV
CCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
ARAVERRIAGEPVHRILGHRDFYGLKLALSPETLEPRPDTETLVDLVLPEARRIANVKKH
HHHHHHHHCCCHHHHHHCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
CRILDLGTGTGAIALALLSVVPEAEAVGTDISEGALETARHNADMSFVAERFTPLRSDWF
CEEEECCCCHHHHHHHHHHHCCCHHHHCCCCHHHHHHHHHHCCCHHHHHHHHCHHHHHHH
RNVDGRFDLIVSNPPYIATAEMETLPREVREHDPKAALDGGPDGLMPYRAIASGARDHLT
HCCCCCEEEEECCCCEEEEHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHCCHHHHHH
REGLIAVETGAEQKAAVAAIFAAEGYAVSRTARDLGGRDRAMLFAPTA
HCCEEEEECCCCHHHHHHHHHHHCCCCHHHHHHHCCCCCCEEEEECCC
>Mature Secondary Structure 
PPITLAALLRTARQQLTSAGVEDAALEARLIVEHFSETTQTQAITHPECAVEAAVAEKV
CCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
ARAVERRIAGEPVHRILGHRDFYGLKLALSPETLEPRPDTETLVDLVLPEARRIANVKKH
HHHHHHHHCCCHHHHHHCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
CRILDLGTGTGAIALALLSVVPEAEAVGTDISEGALETARHNADMSFVAERFTPLRSDWF
CEEEECCCCHHHHHHHHHHHCCCHHHHCCCCHHHHHHHHHHCCCHHHHHHHHCHHHHHHH
RNVDGRFDLIVSNPPYIATAEMETLPREVREHDPKAALDGGPDGLMPYRAIASGARDHLT
HCCCCCEEEEECCCCEEEEHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHCCHHHHHH
REGLIAVETGAEQKAAVAAIFAAEGYAVSRTARDLGGRDRAMLFAPTA
HCCEEEEECCCCHHHHHHHHHHHCCCCHHHHHHHCCCCCCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11214968 [H]