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The map label for this gene is hyuB [H]

Identifier: 110634980

GI number: 110634980

Start: 2859929

End: 2861872

Strand: Reverse

Name: hyuB [H]

Synonym: Meso_2646

Alternate gene names: 110634980

Gene position: 2861872-2859929 (Counterclockwise)

Preceding gene: 110634981

Following gene: 110634979

Centisome position: 64.86

GC content: 59.31

Gene sequence:

>1944_bases
ATGGATCCCGTTTTCACCGAAGTGATGAAACACCGTTTTGCAGCGATCGCCGACGAAGCGAGCAATACGGTCTATCGCAC
GGCGTTCACGACCTTCGTCAAGCAAACGCAGGATTACCAAATCGCGCTTGCGGCAATTGAAGGCGAATTCTTCGCCTTTC
CCGTTCAATCGGGAATGATGTCGAACCTCTGCCACAACATTCGGCCGGTCATCGACATGTTCGGCATCGAAAATCTCAAA
CCGGGCGACGTGATCTTCACCAACGATCCCTTTACCGGGAACGGCCTCGTCACCCACATGATGGATATCCATCTGATCCG
GCCGATCTTCTACAAGGACAAGCTCGTCTGCTTTTCCTGGTCGTTCATCCACGCGTCCGACGTCGGCGGATCCGTGCCGG
GCAGTATCTCTCCAACGAACTCCGAGATCTTCCAGGAGGGCCTGCGCATCGCTCCAACCTTCCTCTACCGGGAGGGCAAA
CTAAACAAGGAGCTGTGGAGGATTTTCGAGGACAACACGCGTATCCCGGACCTCATCTGGGGCGATATCGAGGCCATGGT
GGCGGGCCATCTGCAGCTGGAGCAGCGCGTCACCGAGCTTTGCAATCGCCTCGGTCTGGAAAAGCTCAATCAAGGAATCA
ACGACGTCCTAAAGCTGTCGGAGATCAGGGCGCGCGAAATCATCGGCGCCATGACCGACGGCGTCTATGAGTTTCACGAC
TATCTGGAATCCTATTTCCCCAACGAAGTCATCTTCGTAAACATCAAGATGACGGTGAAGGGCGACGAACTCGAAATAGA
CTACGAGGGAAGCGATCCGCAATTGCCGCTGGCGATGAATCTCGTTGCGGGCGATGCCAAGGCGCACCCCTTCCTCTGCC
ATCCGCTCGTCAACTACATCATCACTGCGGCGGACGACATTCCACGCACGGGCGCCATGCTGCGGTCCGTGACCATCAAG
GCGCCTAAGGGCACGCTTTGCAATGCCGAATTCCCGGCAGCGGGCGGAAACCGCATGGTGTCGGTCAGCCGCTTCTACGA
TGTGCTTCTCGGATGCATGAACCAGGCGGTTCCAGGCGGCCTGGTCTCGGCGGGCTATGGTATCGCGGGTATCATCGTCG
CCTCTTCGCTCGATCCGAAGACGGGTAAGCGACATGTCAGCACGGTCGAGCCATTCATGGGTGGGGGTGGCGGTCGCTGC
AATGGCGACGGCATGGATGCCGTCGACGTGCCGCGTGGCGCTTTGCGAAGCGCGCCGATCGAAACGGTCGAGATCGAGCA
GCCCTTCGTCATTCGCCGGTTCGGCGTTGAGAACGATACGCAGGGTGCCGGCAAGTATAGAGGCGGAGGCGCGGCGGTCC
TGACGATCGAGAACCGGGGGCCTTCCACCATGGTTACGGTTCGCGGTTTGGACCGCTTCGAATTCCAGCCCTGGGGCTTC
GGCGGCGGAAAGTGCGGCCAGAGCGGCTCCGTCATCCTGAATCCGAAGACGGCTGCGGAAAAGGATATCGGAAAGGTCAC
CGTCCTTGAGCTGGTTCCCGGTGACATTCTTCACATGGTCACGCCGACCGGCGGCGGCTTCGGCGACCCGCTCGATCGTG
ACGTGGCCATGGTCCTCAAAGACGTGCAGCAGGAATTCGTCTCGGCGGAACGGGCTCGCAGCGATTACGGCGTCGTCGTG
GAAGGCGGAGCTGTCAACCTGGCGGCGACGGAGAGCTTGCGCTTGCAGATGAAGAAGGGTCTGCGCGACGACACGTATGC
TTATGGAACGAAACGCGTTGAAATCGAAAAGGTATGGGACTCCAAGGCCCGGATCGCCCTTGCCAACGCGGTTTCCCATG
TTCCGGCAACATTGCGTCACAAGGCCAAGACCGATCTCGCCAAGAAGGTGGAGGCCTTGGGTCAGCCGATTACGGCCGCG
CTTGTGAACAGCGTCGCCGCTTGA

Upstream 100 bases:

>100_bases
CCATCATCGAGCAGGAAACCATCACCGTTCTCGTTCCTCCCGCGTTCGACGTGACGATCGGTCGATACGACGACGTCATT
CTTGAAAGAAGGGCATAACA

Downstream 100 bases:

>100_bases
GCTTTTTGGCTCCGTCTGACGAGGCGGAGCCAAAATTGCCTATAGCCGGGGTATTACCATGGGTCAGGCGCAGAAGATTC
TGCACAGCAGTCACTGGGGG

Product: hydantoinase B/oxoprolinase

Products: ADP; phosphate; N-carbamoylsarcosine

Alternate protein names: ORF3 [H]

Number of amino acids: Translated: 647; Mature: 647

Protein sequence:

>647_residues
MDPVFTEVMKHRFAAIADEASNTVYRTAFTTFVKQTQDYQIALAAIEGEFFAFPVQSGMMSNLCHNIRPVIDMFGIENLK
PGDVIFTNDPFTGNGLVTHMMDIHLIRPIFYKDKLVCFSWSFIHASDVGGSVPGSISPTNSEIFQEGLRIAPTFLYREGK
LNKELWRIFEDNTRIPDLIWGDIEAMVAGHLQLEQRVTELCNRLGLEKLNQGINDVLKLSEIRAREIIGAMTDGVYEFHD
YLESYFPNEVIFVNIKMTVKGDELEIDYEGSDPQLPLAMNLVAGDAKAHPFLCHPLVNYIITAADDIPRTGAMLRSVTIK
APKGTLCNAEFPAAGGNRMVSVSRFYDVLLGCMNQAVPGGLVSAGYGIAGIIVASSLDPKTGKRHVSTVEPFMGGGGGRC
NGDGMDAVDVPRGALRSAPIETVEIEQPFVIRRFGVENDTQGAGKYRGGGAAVLTIENRGPSTMVTVRGLDRFEFQPWGF
GGGKCGQSGSVILNPKTAAEKDIGKVTVLELVPGDILHMVTPTGGGFGDPLDRDVAMVLKDVQQEFVSAERARSDYGVVV
EGGAVNLAATESLRLQMKKGLRDDTYAYGTKRVEIEKVWDSKARIALANAVSHVPATLRHKAKTDLAKKVEALGQPITAA
LVNSVAA

Sequences:

>Translated_647_residues
MDPVFTEVMKHRFAAIADEASNTVYRTAFTTFVKQTQDYQIALAAIEGEFFAFPVQSGMMSNLCHNIRPVIDMFGIENLK
PGDVIFTNDPFTGNGLVTHMMDIHLIRPIFYKDKLVCFSWSFIHASDVGGSVPGSISPTNSEIFQEGLRIAPTFLYREGK
LNKELWRIFEDNTRIPDLIWGDIEAMVAGHLQLEQRVTELCNRLGLEKLNQGINDVLKLSEIRAREIIGAMTDGVYEFHD
YLESYFPNEVIFVNIKMTVKGDELEIDYEGSDPQLPLAMNLVAGDAKAHPFLCHPLVNYIITAADDIPRTGAMLRSVTIK
APKGTLCNAEFPAAGGNRMVSVSRFYDVLLGCMNQAVPGGLVSAGYGIAGIIVASSLDPKTGKRHVSTVEPFMGGGGGRC
NGDGMDAVDVPRGALRSAPIETVEIEQPFVIRRFGVENDTQGAGKYRGGGAAVLTIENRGPSTMVTVRGLDRFEFQPWGF
GGGKCGQSGSVILNPKTAAEKDIGKVTVLELVPGDILHMVTPTGGGFGDPLDRDVAMVLKDVQQEFVSAERARSDYGVVV
EGGAVNLAATESLRLQMKKGLRDDTYAYGTKRVEIEKVWDSKARIALANAVSHVPATLRHKAKTDLAKKVEALGQPITAA
LVNSVAA
>Mature_647_residues
MDPVFTEVMKHRFAAIADEASNTVYRTAFTTFVKQTQDYQIALAAIEGEFFAFPVQSGMMSNLCHNIRPVIDMFGIENLK
PGDVIFTNDPFTGNGLVTHMMDIHLIRPIFYKDKLVCFSWSFIHASDVGGSVPGSISPTNSEIFQEGLRIAPTFLYREGK
LNKELWRIFEDNTRIPDLIWGDIEAMVAGHLQLEQRVTELCNRLGLEKLNQGINDVLKLSEIRAREIIGAMTDGVYEFHD
YLESYFPNEVIFVNIKMTVKGDELEIDYEGSDPQLPLAMNLVAGDAKAHPFLCHPLVNYIITAADDIPRTGAMLRSVTIK
APKGTLCNAEFPAAGGNRMVSVSRFYDVLLGCMNQAVPGGLVSAGYGIAGIIVASSLDPKTGKRHVSTVEPFMGGGGGRC
NGDGMDAVDVPRGALRSAPIETVEIEQPFVIRRFGVENDTQGAGKYRGGGAAVLTIENRGPSTMVTVRGLDRFEFQPWGF
GGGKCGQSGSVILNPKTAAEKDIGKVTVLELVPGDILHMVTPTGGGFGDPLDRDVAMVLKDVQQEFVSAERARSDYGVVV
EGGAVNLAATESLRLQMKKGLRDDTYAYGTKRVEIEKVWDSKARIALANAVSHVPATLRHKAKTDLAKKVEALGQPITAA
LVNSVAA

Specific function: HyuA and hyuB convert D- and L-substituted hydantoins to corresponding N-carbamyl-amino acids [H]

COG id: COG0146

COG function: function code EQ; N-methylhydantoinase B/acetone carboxylase, alpha subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the oxoprolinase family [H]

Homologues:

Organism=Homo sapiens, GI48314820, Length=557, Percent_Identity=25.673249551167, Blast_Score=139, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI133901900, Length=570, Percent_Identity=27.5438596491228, Blast_Score=149, Evalue=3e-36,
Organism=Caenorhabditis elegans, GI133901902, Length=544, Percent_Identity=27.3897058823529, Blast_Score=127, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6322634, Length=562, Percent_Identity=26.8683274021352, Blast_Score=152, Evalue=2e-37,
Organism=Drosophila melanogaster, GI45550492, Length=560, Percent_Identity=25.8928571428571, Blast_Score=129, Evalue=8e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003692 [H]

Pfam domain/function: PF02538 Hydantoinase_B [H]

EC number: 3.5.2.14

Molecular weight: Translated: 70306; Mature: 70306

Theoretical pI: Translated: 5.67; Mature: 5.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDPVFTEVMKHRFAAIADEASNTVYRTAFTTFVKQTQDYQIALAAIEGEFFAFPVQSGMM
CCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCEEEEEEECCCEEEEECHHHHH
SNLCHNIRPVIDMFGIENLKPGDVIFTNDPFTGNGLVTHMMDIHLIRPIFYKDKLVCFSW
HHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHCCEEEEEEE
SFIHASDVGGSVPGSISPTNSEIFQEGLRIAPTFLYREGKLNKELWRIFEDNTRIPDLIW
EEEEECCCCCCCCCCCCCCCHHHHHCCHHHCCCEEEECCCCCHHHHHHHHCCCCCCCHHH
GDIEAMVAGHLQLEQRVTELCNRLGLEKLNQGINDVLKLSEIRAREIIGAMTDGVYEFHD
CCHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YLESYFPNEVIFVNIKMTVKGDELEIDYEGSDPQLPLAMNLVAGDAKAHPFLCHPLVNYI
HHHHHCCCCEEEEEEEEEEECCEEEEEECCCCCCCCEEEEEEECCCCCCCEEHHHHHHHH
ITAADDIPRTGAMLRSVTIKAPKGTLCNAEFPAAGGNRMVSVSRFYDVLLGCMNQAVPGG
HHCCCCCCCCCHHEEEEEEECCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHCCCCC
LVSAGYGIAGIIVASSLDPKTGKRHVSTVEPFMGGGGGRCNGDGMDAVDVPRGALRSAPI
HHHCCCCEEEEEEECCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCHHHHCCCC
ETVEIEQPFVIRRFGVENDTQGAGKYRGGGAAVLTIENRGPSTMVTVRGLDRFEFQPWGF
CEEECCCCEEEEEECCCCCCCCCCCCCCCCEEEEEEECCCCCEEEEEECCCCCCCCCCCC
GGGKCGQSGSVILNPKTAAEKDIGKVTVLELVPGDILHMVTPTGGGFGDPLDRDVAMVLK
CCCCCCCCCCEEECCCCCCHHCCCCEEEEEECCCCEEEEECCCCCCCCCCCHHHHHHHHH
DVQQEFVSAERARSDYGVVVEGGAVNLAATESLRLQMKKGLRDDTYAYGTKRVEIEKVWD
HHHHHHHHHHHCCCCCCEEEECCEEEEEEHHHHHHHHHHCCCCCHHHCCCEEEEEEECCC
SKARIALANAVSHVPATLRHKAKTDLAKKVEALGQPITAALVNSVAA
CCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCC
>Mature Secondary Structure
MDPVFTEVMKHRFAAIADEASNTVYRTAFTTFVKQTQDYQIALAAIEGEFFAFPVQSGMM
CCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCEEEEEEECCCEEEEECHHHHH
SNLCHNIRPVIDMFGIENLKPGDVIFTNDPFTGNGLVTHMMDIHLIRPIFYKDKLVCFSW
HHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHCCEEEEEEE
SFIHASDVGGSVPGSISPTNSEIFQEGLRIAPTFLYREGKLNKELWRIFEDNTRIPDLIW
EEEEECCCCCCCCCCCCCCCHHHHHCCHHHCCCEEEECCCCCHHHHHHHHCCCCCCCHHH
GDIEAMVAGHLQLEQRVTELCNRLGLEKLNQGINDVLKLSEIRAREIIGAMTDGVYEFHD
CCHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YLESYFPNEVIFVNIKMTVKGDELEIDYEGSDPQLPLAMNLVAGDAKAHPFLCHPLVNYI
HHHHHCCCCEEEEEEEEEEECCEEEEEECCCCCCCCEEEEEEECCCCCCCEEHHHHHHHH
ITAADDIPRTGAMLRSVTIKAPKGTLCNAEFPAAGGNRMVSVSRFYDVLLGCMNQAVPGG
HHCCCCCCCCCHHEEEEEEECCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHCCCCC
LVSAGYGIAGIIVASSLDPKTGKRHVSTVEPFMGGGGGRCNGDGMDAVDVPRGALRSAPI
HHHCCCCEEEEEEECCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCHHHHCCCC
ETVEIEQPFVIRRFGVENDTQGAGKYRGGGAAVLTIENRGPSTMVTVRGLDRFEFQPWGF
CEEECCCCEEEEEECCCCCCCCCCCCCCCCEEEEEEECCCCCEEEEEECCCCCCCCCCCC
GGGKCGQSGSVILNPKTAAEKDIGKVTVLELVPGDILHMVTPTGGGFGDPLDRDVAMVLK
CCCCCCCCCCEEECCCCCCHHCCCCEEEEEECCCCEEEEECCCCCCCCCCCHHHHHHHHH
DVQQEFVSAERARSDYGVVVEGGAVNLAATESLRLQMKKGLRDDTYAYGTKRVEIEKVWD
HHHHHHHHHHHCCCCCCEEEECCEEEEEEHHHHHHHHHHCCCCCHHHCCCEEEEEEECCC
SKARIALANAVSHVPATLRHKAKTDLAKKVEALGQPITAALVNSVAA
CCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; N-methylimidazolidine-2,4-dione; H2O

Specific reaction: ATP + N-methylimidazolidine-2,4-dione + 2 H2O = ADP + phosphate + N-carbamoylsarcosine

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1732229 [H]