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The map label for this gene is thiE [H]

Identifier: 110634906

GI number: 110634906

Start: 2754348

End: 2754968

Strand: Direct

Name: thiE [H]

Synonym: Meso_2565

Alternate gene names: 110634906

Gene position: 2754348-2754968 (Clockwise)

Preceding gene: 110634905

Following gene: 110634907

Centisome position: 62.42

GC content: 60.87

Gene sequence:

>621_bases
ATGAGGCTCGACCCGTTTTATCTCATCGTCGATTCTGCAGCCTGGATCGAGCGCCTCGTGCCACTTGGCGTAAAGCTGCT
GCAACTTCGCATCAAGGAAATTGACGAAGCAGAGCTCCGGGCGGAAATCCGCCGCGCGAGAAAAATATGCGCAGGGCATG
ATTGCCAGCTCATCGTGAACGATTACTGGCAGCTCGCCATCGAGGAGGGCTGCGACTTCGTCCATCTGGGCCAGGAGGAT
TTGGCGACCGCCGACCTTGCCGCGATCAAGCGGGCGGGGCTGAAATTTGGGATCAGCACGCATGACCACACGGAACTTGC
GACAGCGCTGAAGGCCGAACCCGACTATATCGCGCTCGGGCCGATCTACCCAACCATCCTCAAGAAGATGAAATGGGAGC
CGCAAGGGCTCGACCGTATCGCCGAATGGAAGGAACGCGTAGCCCCAGTGCCGCTCGTCGCCATCGGCGGCCTGACGCCG
GAGCGCATCCCCGGCGTTTTCGAGCGCGGCGCGGATTCGGCGGCCGTGGTGACGGACATATTGCGCAATTCCAACCCCGA
GGCGCGCACACGGGAATGGTTAGAGGCGACCGCATCATGGAGGGAAAAAAGCCGAGCATGA

Upstream 100 bases:

>100_bases
AAGCGGTGGAGGCGGGACATGCGGCCTACGGCGCCGGCATATTGGAGCCGCGCGACATGGCCGTGCCTTCCACGCCCGTG
ATCGGCAAGGCGGTGTTCTC

Downstream 100 bases:

>100_bases
GTGTCAGGCCGCATGTGCTTGTCGTCGCCGGCTCTGATTCCTCCGGAGGAGCAGGAAACGGCCGCGACATCGAAGTCCTT
GCCAGCCTGGGCGTTAACGC

Product: thiamine-phosphate pyrophosphorylase

Products: NA

Alternate protein names: TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase [H]

Number of amino acids: Translated: 206; Mature: 206

Protein sequence:

>206_residues
MRLDPFYLIVDSAAWIERLVPLGVKLLQLRIKEIDEAELRAEIRRARKICAGHDCQLIVNDYWQLAIEEGCDFVHLGQED
LATADLAAIKRAGLKFGISTHDHTELATALKAEPDYIALGPIYPTILKKMKWEPQGLDRIAEWKERVAPVPLVAIGGLTP
ERIPGVFERGADSAAVVTDILRNSNPEARTREWLEATASWREKSRA

Sequences:

>Translated_206_residues
MRLDPFYLIVDSAAWIERLVPLGVKLLQLRIKEIDEAELRAEIRRARKICAGHDCQLIVNDYWQLAIEEGCDFVHLGQED
LATADLAAIKRAGLKFGISTHDHTELATALKAEPDYIALGPIYPTILKKMKWEPQGLDRIAEWKERVAPVPLVAIGGLTP
ERIPGVFERGADSAAVVTDILRNSNPEARTREWLEATASWREKSRA
>Mature_206_residues
MRLDPFYLIVDSAAWIERLVPLGVKLLQLRIKEIDEAELRAEIRRARKICAGHDCQLIVNDYWQLAIEEGCDFVHLGQED
LATADLAAIKRAGLKFGISTHDHTELATALKAEPDYIALGPIYPTILKKMKWEPQGLDRIAEWKERVAPVPLVAIGGLTP
ERIPGVFERGADSAAVVTDILRNSNPEARTREWLEATASWREKSRA

Specific function: Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP) [H]

COG id: COG0352

COG function: function code H; Thiamine monophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TMP-PPase family [H]

Homologues:

Organism=Escherichia coli, GI1790426, Length=190, Percent_Identity=46.3157894736842, Blast_Score=166, Evalue=1e-42,
Organism=Saccharomyces cerevisiae, GI6325042, Length=225, Percent_Identity=30.6666666666667, Blast_Score=75, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR022998
- InterPro:   IPR003733 [H]

Pfam domain/function: PF02581 TMP-TENI [H]

EC number: =2.5.1.3 [H]

Molecular weight: Translated: 23145; Mature: 23145

Theoretical pI: Translated: 5.75; Mature: 5.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLDPFYLIVDSAAWIERLVPLGVKLLQLRIKEIDEAELRAEIRRARKICAGHDCQLIVN
CCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCEEHHH
DYWQLAIEEGCDFVHLGQEDLATADLAAIKRAGLKFGISTHDHTELATALKAEPDYIALG
HHHHHHHHHCCCEEEECHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCEEEEC
PIYPTILKKMKWEPQGLDRIAEWKERVAPVPLVAIGGLTPERIPGVFERGADSAAVVTDI
CHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCEEEECCCCHHHCCHHHHCCCCHHHHHHHH
LRNSNPEARTREWLEATASWREKSRA
HHCCCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRLDPFYLIVDSAAWIERLVPLGVKLLQLRIKEIDEAELRAEIRRARKICAGHDCQLIVN
CCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCEEHHH
DYWQLAIEEGCDFVHLGQEDLATADLAAIKRAGLKFGISTHDHTELATALKAEPDYIALG
HHHHHHHHHCCCEEEECHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCEEEEC
PIYPTILKKMKWEPQGLDRIAEWKERVAPVPLVAIGGLTPERIPGVFERGADSAAVVTDI
CHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCEEEECCCCHHHCCHHHHCCCCHHHHHHHH
LRNSNPEARTREWLEATASWREKSRA
HHCCCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9371431 [H]