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The map label for this gene is thiG [H]

Identifier: 110634905

GI number: 110634905

Start: 2753575

End: 2754351

Strand: Direct

Name: thiG [H]

Synonym: Meso_2564

Alternate gene names: 110634905

Gene position: 2753575-2754351 (Clockwise)

Preceding gene: 110634904

Following gene: 110634906

Centisome position: 62.4

GC content: 65.64

Gene sequence:

>777_bases
ATGCCGCTCACCGTCTATGGAACCGAACTTTCCTCCCGCCTGCTGCTGGGCACCGCGCAATATCCCTCGCCCGCCATCCT
TTCGGAAGCAGCGAAGTCTTCCGGTGCGTCGGTTGTCACCGTATCGTTGCGGCGCGAGGGCATTCAGGGGGAAACGGGTG
GTCGCTTCTGGTCGCTTGTCCGCGAGCTCGGCCTGCGCGTGCTGCCCAACACGGCGGGTTGCCACACGGTCAAGGAAGCG
GTGACCACGGCCAAAATGGCGCGGGAAGTGTTCGGGACGAACTGGATCAAGCTTGAGGTGATCGGCCATCACGACACGCT
GCAGCCCGATGTTTTCGGCCTAGTGGAAGCCGCGCGCATCCTCACCGAGGATGGCTTTGCCGTCTTCCCTTACACGACCG
AGGACCTCGTCGTGGGCGAGCGGCTGATCGAGGCGGGCTGCGAAGTGCTGATGCCCTGGTGCGCACCGATCGGCTCGGCC
AAGGGTCCGGTCAATCTCGACGCGCTGCGCGCCTACCGAGCACATTTTCCGGACACGTCGCTGATTGTCGACGCCGGGCT
CGGGCGGCCTTCGCATGCCGCAGCCGTGATGGAACTGGGCTTCGACGGCGTACTGCTCAATACGGCGGTCGCCAAGGCAG
GGAACCCCGCCGCCATGGCCATCGCCTTCGCGCAAGCGGTGGAGGCGGGACATGCGGCCTACGGCGCCGGCATATTGGAG
CCGCGCGACATGGCCGTGCCTTCCACGCCCGTGATCGGCAAGGCGGTGTTCTCATGA

Upstream 100 bases:

>100_bases
CCATCAATTCCGAATTCGTGCCAGGCAGCGAGCGCAACGAAGTCAGGCTTTCCGAGGGCGACCGCGTGGAAATCCTGACG
CCCAGGCAAGGAGGCTAAAA

Downstream 100 bases:

>100_bases
GGCTCGACCCGTTTTATCTCATCGTCGATTCTGCAGCCTGGATCGAGCGCCTCGTGCCACTTGGCGTAAAGCTGCTGCAA
CTTCGCATCAAGGAAATTGA

Product: thiazole synthase

Products: 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate; 4-hydroxy-benzyl-alcohol; C1 of tyrosine; ThiS protein [C]

Alternate protein names: NA

Number of amino acids: Translated: 258; Mature: 257

Protein sequence:

>258_residues
MPLTVYGTELSSRLLLGTAQYPSPAILSEAAKSSGASVVTVSLRREGIQGETGGRFWSLVRELGLRVLPNTAGCHTVKEA
VTTAKMAREVFGTNWIKLEVIGHHDTLQPDVFGLVEAARILTEDGFAVFPYTTEDLVVGERLIEAGCEVLMPWCAPIGSA
KGPVNLDALRAYRAHFPDTSLIVDAGLGRPSHAAAVMELGFDGVLLNTAVAKAGNPAAMAIAFAQAVEAGHAAYGAGILE
PRDMAVPSTPVIGKAVFS

Sequences:

>Translated_258_residues
MPLTVYGTELSSRLLLGTAQYPSPAILSEAAKSSGASVVTVSLRREGIQGETGGRFWSLVRELGLRVLPNTAGCHTVKEA
VTTAKMAREVFGTNWIKLEVIGHHDTLQPDVFGLVEAARILTEDGFAVFPYTTEDLVVGERLIEAGCEVLMPWCAPIGSA
KGPVNLDALRAYRAHFPDTSLIVDAGLGRPSHAAAVMELGFDGVLLNTAVAKAGNPAAMAIAFAQAVEAGHAAYGAGILE
PRDMAVPSTPVIGKAVFS
>Mature_257_residues
PLTVYGTELSSRLLLGTAQYPSPAILSEAAKSSGASVVTVSLRREGIQGETGGRFWSLVRELGLRVLPNTAGCHTVKEAV
TTAKMAREVFGTNWIKLEVIGHHDTLQPDVFGLVEAARILTEDGFAVFPYTTEDLVVGERLIEAGCEVLMPWCAPIGSAK
GPVNLDALRAYRAHFPDTSLIVDAGLGRPSHAAAVMELGFDGVLLNTAVAKAGNPAAMAIAFAQAVEAGHAAYGAGILEP
RDMAVPSTPVIGKAVFS

Specific function: Catalyzes the rearrangement of 1-deoxy-D-xylulose 5- phosphate (DXP) to produce the thiazole phosphate moiety of thiamine. Sulfur is provided by the thiocarboxylate moiety of the carrier protein ThiS. In vitro, sulfur can be provided by H(2)S [H]

COG id: COG2022

COG function: function code H; Uncharacterized enzyme of thiazole biosynthesis

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiG family [H]

Homologues:

Organism=Escherichia coli, GI48994993, Length=235, Percent_Identity=39.5744680851064, Blast_Score=161, Evalue=5e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR008867 [H]

Pfam domain/function: PF05690 ThiG [H]

EC number: NA

Molecular weight: Translated: 27051; Mature: 26920

Theoretical pI: Translated: 5.61; Mature: 5.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLTVYGTELSSRLLLGTAQYPSPAILSEAAKSSGASVVTVSLRREGIQGETGGRFWSLV
CCEEEECCCCCCEEEEEECCCCCHHHHHHHHHCCCCEEEEEEEECCCCCCCCCHHHHHHH
RELGLRVLPNTAGCHTVKEAVTTAKMAREVFGTNWIKLEVIGHHDTLQPDVFGLVEAARI
HHCCCEECCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHHHH
LTEDGFAVFPYTTEDLVVGERLIEAGCEVLMPWCAPIGSAKGPVNLDALRAYRAHFPDTS
HHCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCC
LIVDAGLGRPSHAAAVMELGFDGVLLNTAVAKAGNPAAMAIAFAQAVEAGHAAYGAGILE
EEEECCCCCCHHHHHHHHHCCCCEEHHHHHHCCCCCHHHHHHHHHHHHHCCHHHCCCCCC
PRDMAVPSTPVIGKAVFS
CCCCCCCCCCCCHHHHCC
>Mature Secondary Structure 
PLTVYGTELSSRLLLGTAQYPSPAILSEAAKSSGASVVTVSLRREGIQGETGGRFWSLV
CEEEECCCCCCEEEEEECCCCCHHHHHHHHHCCCCEEEEEEEECCCCCCCCCHHHHHHH
RELGLRVLPNTAGCHTVKEAVTTAKMAREVFGTNWIKLEVIGHHDTLQPDVFGLVEAARI
HHCCCEECCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHHHH
LTEDGFAVFPYTTEDLVVGERLIEAGCEVLMPWCAPIGSAKGPVNLDALRAYRAHFPDTS
HHCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCC
LIVDAGLGRPSHAAAVMELGFDGVLLNTAVAKAGNPAAMAIAFAQAVEAGHAAYGAGILE
EEEECCCCCCHHHHHHHHHCCCCEEHHHHHHCCCCCHHHHHHHHHHHHHCCHHHCCCCCC
PRDMAVPSTPVIGKAVFS
CCCCCCCCCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: deoxyxylulose-5-phosphate; ThiS-COSH; L-tyrosine [C]

Specific reaction: deoxyxylulose-5-phosphate + ThiS-COSH + L-tyrosine = 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate + 4-hydroxy-benzyl-alcohol + C1 of tyrosine + ThiS protein [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11214968 [H]