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The map label for this gene is acoA [H]

Identifier: 110634612

GI number: 110634612

Start: 2419455

End: 2420582

Strand: Direct

Name: acoA [H]

Synonym: Meso_2266

Alternate gene names: 110634612

Gene position: 2419455-2420582 (Clockwise)

Preceding gene: 110634611

Following gene: 110634613

Centisome position: 54.83

GC content: 64.36

Gene sequence:

>1128_bases
ATGGCGGGCGCAGCCGTGTCCTTCGGCACCACGCTCGCACGGGCAAACGGCGAGATCGGTTTTTGGGCAAAGGACCTTTC
GGACCAGCAGCTCGTCGACATGTTCACGACCATCCTGCGCATCCGTTGGCACGAGCGCACCATGGCCGACAAGATGCTGA
CGGACCCGAACTATCGCGGCTACAACCACTTCTACGCGGGCCAGGAGGCAGTCGCCGTCGGCGTCTGCTCAGCGCTGCGC
AACTCCGGCGGCGTGATGCATGCCGATCTGGCCTATTCGACGCACCGGCCGACCGGCCATGCGATCGCCAAAGGCGTGGA
CATGAAGCTGATGGCGGCGGAGAACGACTTCCGCGCCACCGGCCTCAACGGCGGCTATGCGGCCGAAATGCACCTCTGCG
ATCCGGAAGTGGGCTTCATAGGCGCGGACGGCATGATCGGTCCGGGCCCGGTGATCGCCAGCGGCTCCGCCTTCGCCATC
AAGGCGCGCGGCAGCGACCAGGTGGTGGTGAATTTCGGTGGTGACGGCACCTATGCGACACCGCATTTTCACTCGACGCT
GAACAACGCCGCACTGTTGAAGCTGCCCTTCATCTACGTGCTGGAGAACAACCTCTATCACCAGTATGCGCACTACTCCT
ATTCGTGCCCGCTGAAGGACATTGCGGATGCCGCGAACACCTACGGCATTCCCGGCATCGTCGTGGACGGGCAGGATGTG
ATCCAGGTCTACAATGCCGCCAAGACGGCCGTCGACCGGGCGAGGGCTGGCGAAGGGCCGACGCTGATCGAGGCCAAGAC
CTATCGCTACTACAACCATTGGGGTGCGCCCGGCGCCAAGCCCGGCGAGCTCGGCGCCTTCGGTTACGATCCGCTTGCCA
TCTCTTCCTTCCGGCCGGAGCGGGAGCTGCGCTCCTGGATGCAACGCGACCCCGTCAGGATCGCTCACGACATCCTCGTC
AATTGGGGCGTGATCGACCATGCCAAGGCCGCCGAGATCGAAGCTTCGGTGAAGCAGGACGTCGCGGACGCCTTCGCCTG
GGCGGCTGAGCAGCCCCTCTGCAAGGCGGAAGACGGGCTGAAGAACGTCTTCGTCGAAGGCACCGTCGAGGCGCGCCAGT
TCGGTTGA

Upstream 100 bases:

>100_bases
AGACGATGTCATGCATGACAATCCAGATCGCAAGTCGGACGGTGACGAGCACATTTCTCCGCTTGGAACCTCGCGCCGGA
GCTTCTTGAAGGCCGCCGCC

Downstream 100 bases:

>100_bases
GAGGCGACGTCGGCAGGTCCGGTCTCCCTCTCCCTGGCAGCAATTGGAAAGGTACGTGAAATGGCACGCAAAAGCTGGAT
GTACTCGGTCCTTGAGGCCG

Product: twin-arginine translocation pathway signal

Products: [dihydrolipoyllysine-residue acetyltransferase] ; $S-acetyldihydrolipoyllysine; CO2

Alternate protein names: Acetoin:DCPIP oxidoreductase-alpha; Ao:DCPIP OR; TPP-dependent acetoin dehydrogenase E1 subunit alpha [H]

Number of amino acids: Translated: 375; Mature: 374

Protein sequence:

>375_residues
MAGAAVSFGTTLARANGEIGFWAKDLSDQQLVDMFTTILRIRWHERTMADKMLTDPNYRGYNHFYAGQEAVAVGVCSALR
NSGGVMHADLAYSTHRPTGHAIAKGVDMKLMAAENDFRATGLNGGYAAEMHLCDPEVGFIGADGMIGPGPVIASGSAFAI
KARGSDQVVVNFGGDGTYATPHFHSTLNNAALLKLPFIYVLENNLYHQYAHYSYSCPLKDIADAANTYGIPGIVVDGQDV
IQVYNAAKTAVDRARAGEGPTLIEAKTYRYYNHWGAPGAKPGELGAFGYDPLAISSFRPERELRSWMQRDPVRIAHDILV
NWGVIDHAKAAEIEASVKQDVADAFAWAAEQPLCKAEDGLKNVFVEGTVEARQFG

Sequences:

>Translated_375_residues
MAGAAVSFGTTLARANGEIGFWAKDLSDQQLVDMFTTILRIRWHERTMADKMLTDPNYRGYNHFYAGQEAVAVGVCSALR
NSGGVMHADLAYSTHRPTGHAIAKGVDMKLMAAENDFRATGLNGGYAAEMHLCDPEVGFIGADGMIGPGPVIASGSAFAI
KARGSDQVVVNFGGDGTYATPHFHSTLNNAALLKLPFIYVLENNLYHQYAHYSYSCPLKDIADAANTYGIPGIVVDGQDV
IQVYNAAKTAVDRARAGEGPTLIEAKTYRYYNHWGAPGAKPGELGAFGYDPLAISSFRPERELRSWMQRDPVRIAHDILV
NWGVIDHAKAAEIEASVKQDVADAFAWAAEQPLCKAEDGLKNVFVEGTVEARQFG
>Mature_374_residues
AGAAVSFGTTLARANGEIGFWAKDLSDQQLVDMFTTILRIRWHERTMADKMLTDPNYRGYNHFYAGQEAVAVGVCSALRN
SGGVMHADLAYSTHRPTGHAIAKGVDMKLMAAENDFRATGLNGGYAAEMHLCDPEVGFIGADGMIGPGPVIASGSAFAIK
ARGSDQVVVNFGGDGTYATPHFHSTLNNAALLKLPFIYVLENNLYHQYAHYSYSCPLKDIADAANTYGIPGIVVDGQDVI
QVYNAAKTAVDRARAGEGPTLIEAKTYRYYNHWGAPGAKPGELGAFGYDPLAISSFRPERELRSWMQRDPVRIAHDILVN
WGVIDHAKAAEIEASVKQDVADAFAWAAEQPLCKAEDGLKNVFVEGTVEARQFG

Specific function: Catalyzes the 2,6-dichlorophenolindophenol-dependent cleavage of acetoin into acetate and acetaldehyde. The alpha subunit is probably the catalytic subunit of the enzyme [H]

COG id: COG1071

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, alpha subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI4885543, Length=328, Percent_Identity=30.1829268292683, Blast_Score=140, Evalue=1e-33,
Organism=Homo sapiens, GI4505685, Length=325, Percent_Identity=28.3076923076923, Blast_Score=123, Evalue=3e-28,
Organism=Homo sapiens, GI291084742, Length=325, Percent_Identity=28.3076923076923, Blast_Score=122, Evalue=4e-28,
Organism=Homo sapiens, GI291084744, Length=332, Percent_Identity=27.710843373494, Blast_Score=116, Evalue=4e-26,
Organism=Homo sapiens, GI291084757, Length=325, Percent_Identity=26.1538461538462, Blast_Score=95, Evalue=9e-20,
Organism=Homo sapiens, GI11386135, Length=334, Percent_Identity=26.9461077844311, Blast_Score=91, Evalue=1e-18,
Organism=Homo sapiens, GI258645172, Length=334, Percent_Identity=26.9461077844311, Blast_Score=88, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI32564172, Length=301, Percent_Identity=27.5747508305648, Blast_Score=106, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17536047, Length=301, Percent_Identity=27.5747508305648, Blast_Score=106, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI86563355, Length=360, Percent_Identity=26.3888888888889, Blast_Score=101, Evalue=5e-22,
Organism=Caenorhabditis elegans, GI86563357, Length=360, Percent_Identity=26.3888888888889, Blast_Score=101, Evalue=6e-22,
Organism=Saccharomyces cerevisiae, GI6321026, Length=347, Percent_Identity=27.9538904899135, Blast_Score=123, Evalue=6e-29,
Organism=Drosophila melanogaster, GI24639744, Length=328, Percent_Identity=29.2682926829268, Blast_Score=115, Evalue=5e-26,
Organism=Drosophila melanogaster, GI28571106, Length=328, Percent_Identity=29.2682926829268, Blast_Score=115, Evalue=5e-26,
Organism=Drosophila melanogaster, GI24639740, Length=328, Percent_Identity=29.2682926829268, Blast_Score=115, Evalue=5e-26,
Organism=Drosophila melanogaster, GI24639746, Length=319, Percent_Identity=29.4670846394984, Blast_Score=110, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24639748, Length=318, Percent_Identity=29.2452830188679, Blast_Score=110, Evalue=2e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001017 [H]

Pfam domain/function: PF00676 E1_dh [H]

EC number: 1.2.4.1

Molecular weight: Translated: 40580; Mature: 40449

Theoretical pI: Translated: 6.04; Mature: 6.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGAAVSFGTTLARANGEIGFWAKDLSDQQLVDMFTTILRIRWHERTMADKMLTDPNYRG
CCCCEEECCHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
YNHFYAGQEAVAVGVCSALRNSGGVMHADLAYSTHRPTGHAIAKGVDMKLMAAENDFRAT
CCCCCCCCHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCHHHHCCCCEEEEEECCCCEEE
GLNGGYAAEMHLCDPEVGFIGADGMIGPGPVIASGSAFAIKARGSDQVVVNFGGDGTYAT
CCCCCEEEEEEECCCCCCEEECCCCCCCCCEEECCCEEEEEECCCCEEEEEECCCCCEEC
PHFHSTLNNAALLKLPFIYVLENNLYHQYAHYSYSCPLKDIADAANTYGIPGIVVDGQDV
CHHHHHCCCCEEEEEEEEEEEECCCCHHHHCEEECCCHHHHHHHHHHCCCCEEEECCHHH
IQVYNAAKTAVDRARAGEGPTLIEAKTYRYYNHWGAPGAKPGELGAFGYDPLAISSFRPE
HHHHHHHHHHHHHHHCCCCCEEEEEEEEEEEECCCCCCCCCCCCCCCCCCCEEECCCCCH
RELRSWMQRDPVRIAHDILVNWGVIDHAKAAEIEASVKQDVADAFAWAAEQPLCKAEDGL
HHHHHHHHCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCH
KNVFVEGTVEARQFG
HHEEEECEECCCCCC
>Mature Secondary Structure 
AGAAVSFGTTLARANGEIGFWAKDLSDQQLVDMFTTILRIRWHERTMADKMLTDPNYRG
CCCEEECCHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
YNHFYAGQEAVAVGVCSALRNSGGVMHADLAYSTHRPTGHAIAKGVDMKLMAAENDFRAT
CCCCCCCCHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCHHHHCCCCEEEEEECCCCEEE
GLNGGYAAEMHLCDPEVGFIGADGMIGPGPVIASGSAFAIKARGSDQVVVNFGGDGTYAT
CCCCCEEEEEEECCCCCCEEECCCCCCCCCEEECCCEEEEEECCCCEEEEEECCCCCEEC
PHFHSTLNNAALLKLPFIYVLENNLYHQYAHYSYSCPLKDIADAANTYGIPGIVVDGQDV
CHHHHHCCCCEEEEEEEEEEEECCCCHHHHCEEECCCHHHHHHHHHHCCCCEEEECCHHH
IQVYNAAKTAVDRARAGEGPTLIEAKTYRYYNHWGAPGAKPGELGAFGYDPLAISSFRPE
HHHHHHHHHHHHHHHCCCCCEEEEEEEEEEEECCCCCCCCCCCCCCCCCCCEEECCCCCH
RELRSWMQRDPVRIAHDILVNWGVIDHAKAAEIEASVKQDVADAFAWAAEQPLCKAEDGL
HHHHHHHHCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCH
KNVFVEGTVEARQFG
HHEEEECEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: pyruvate; [dihydrolipoyllysine-residue acetyltransferase] lipoyllysine

Specific reaction: pyruvate + [dihydrolipoyllysine-residue acetyltransferase] lipoyllysine = [dihydrolipoyllysine-residue acetyltransferase] S- $acetyldihydrolipoyllysine + CO2

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969503; 10368162; 9384377 [H]