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The map label for this gene is carA [H]

Identifier: 110634433

GI number: 110634433

Start: 2235613

End: 2236812

Strand: Direct

Name: carA [H]

Synonym: Meso_2083

Alternate gene names: 110634433

Gene position: 2235613-2236812 (Clockwise)

Preceding gene: 110634431

Following gene: 110634437

Centisome position: 50.67

GC content: 63.83

Gene sequence:

>1200_bases
ATGGCATCAACCGCGCCTTGGAGTGAAACCAGGCCGACGGCACTTCTGGTCCTGGCCGACGGCACGGTGATCGAGGGCCG
AGGCCTGGGTGCGGCCGGCTCGGCGGTGGGCGAGGTGTGCTTCAACACTGCCTTGACCGGATATCAGGAGATTCTCACCG
ACCCGTCCTATGCGGGCCAGATCGTCACCTTCACCTTCCCGCATATCGGGAATGTGGGCACCAATGACGAGGATATCGAG
GATCTCAATCCCGCCGCGCGCGCCGGTGCTGTCGGCGCGATCTTCAAGGCCGACGTCACCGATCCGTCCAACTACCGCGC
GGGAGAGCATCTTGACCGCTGGCTGAAGCGGCGCGGTATCGTGGCGCTTGCCGGCATCGACACGCGCGCGCTCACCTCCC
TCCTTCGCGAGAAAGGCGCTGCCAATGCCGTTATCGCCCACGCGCCGGACGGGCGCTTCGATGTGGAAGCGCTGAAGAAG
GAAGCCAGCGAATGGGGTGGTCTTGTAGGGCTCGATCTGGCCAAGGTCGTCACCTCCGGCCAGTCGTCCACTTGGACTGA
GACCCCCTGGGTCTGGAGCGAGGGCTATGCCGAGGCCCGCGAGACGCCCTATCACATCGTCACCGTCGATTACGGCGTGA
AGCGCAATATCCTGCGAATGCTGTCCAGCCTGGGCGCAAAGGTCACCGTCGTGCCGGCCACCGCTTCCGCCGAGGATATT
CTGGCCATGCGGCCTGACGGCGTGTTCCTGTCGAACGGCCCCGGCGATCCTGCGGCCACGGGCGCATACGCCGTGCCCAT
GATCCGTGAATTGCTCAAGACGGAGACCCCGGTTTTCGGCATTTGCCTGGGCCATCAAATGTTGGCTCTGGCGCTGGGCG
CCAGGACGGTCAAAATGCATCAGGGCCATCACGGCGCCAATCACCCCGTCAAGGATTTCACCACCGGCAAGGTGGAAATC
GTGTCCATGAATCACGGCTTTGCCGTCGATAGCGATAGCCTGCCGGAAGGAGTGGAGGAGACGCATGTCTCCCTTTTCGA
CGGCTCCAATTGCGGCATCAGCCTCGCCGGCCGGCCGGTTTTCTCGGTGCAGCATCATCCGGAAGCCTCCCCCGGCCCGC
AGGACTCGCATTACCTTTTCCGCCGATTCATCAATCTGGTGCGCGAGAAGAAGGGCGAGCCGGCGCTGGCAGAGCGCTGA

Upstream 100 bases:

>100_bases
GCGAAGGTTGCGGTTTAGCCGTCGCAGTCGGCATTTCGTGCGCCATATGGCCTGCCACGGGCCTTTTTTCAAGCCCTGCG
AGAGTAATGAGGAGAGCGAC

Downstream 100 bases:

>100_bases
GAGCGAACTTCTCATCAAGAAGGCGGCCTCCGGGCCGCCTTTTTTGTTTCGGCTAAAGGATAGGCGTCAGCGCATCGCCA
GCCGTTTTGAGCAAAGAGGG

Product: carbamoyl phosphate synthase small subunit

Products: NA

Alternate protein names: Carbamoyl-phosphate synthetase glutamine chain [H]

Number of amino acids: Translated: 399; Mature: 398

Protein sequence:

>399_residues
MASTAPWSETRPTALLVLADGTVIEGRGLGAAGSAVGEVCFNTALTGYQEILTDPSYAGQIVTFTFPHIGNVGTNDEDIE
DLNPAARAGAVGAIFKADVTDPSNYRAGEHLDRWLKRRGIVALAGIDTRALTSLLREKGAANAVIAHAPDGRFDVEALKK
EASEWGGLVGLDLAKVVTSGQSSTWTETPWVWSEGYAEARETPYHIVTVDYGVKRNILRMLSSLGAKVTVVPATASAEDI
LAMRPDGVFLSNGPGDPAATGAYAVPMIRELLKTETPVFGICLGHQMLALALGARTVKMHQGHHGANHPVKDFTTGKVEI
VSMNHGFAVDSDSLPEGVEETHVSLFDGSNCGISLAGRPVFSVQHHPEASPGPQDSHYLFRRFINLVREKKGEPALAER

Sequences:

>Translated_399_residues
MASTAPWSETRPTALLVLADGTVIEGRGLGAAGSAVGEVCFNTALTGYQEILTDPSYAGQIVTFTFPHIGNVGTNDEDIE
DLNPAARAGAVGAIFKADVTDPSNYRAGEHLDRWLKRRGIVALAGIDTRALTSLLREKGAANAVIAHAPDGRFDVEALKK
EASEWGGLVGLDLAKVVTSGQSSTWTETPWVWSEGYAEARETPYHIVTVDYGVKRNILRMLSSLGAKVTVVPATASAEDI
LAMRPDGVFLSNGPGDPAATGAYAVPMIRELLKTETPVFGICLGHQMLALALGARTVKMHQGHHGANHPVKDFTTGKVEI
VSMNHGFAVDSDSLPEGVEETHVSLFDGSNCGISLAGRPVFSVQHHPEASPGPQDSHYLFRRFINLVREKKGEPALAER
>Mature_398_residues
ASTAPWSETRPTALLVLADGTVIEGRGLGAAGSAVGEVCFNTALTGYQEILTDPSYAGQIVTFTFPHIGNVGTNDEDIED
LNPAARAGAVGAIFKADVTDPSNYRAGEHLDRWLKRRGIVALAGIDTRALTSLLREKGAANAVIAHAPDGRFDVEALKKE
ASEWGGLVGLDLAKVVTSGQSSTWTETPWVWSEGYAEARETPYHIVTVDYGVKRNILRMLSSLGAKVTVVPATASAEDIL
AMRPDGVFLSNGPGDPAATGAYAVPMIRELLKTETPVFGICLGHQMLALALGARTVKMHQGHHGANHPVKDFTTGKVEIV
SMNHGFAVDSDSLPEGVEETHVSLFDGSNCGISLAGRPVFSVQHHPEASPGPQDSHYLFRRFINLVREKKGEPALAER

Specific function: Arginine biosynthesis. Pyrimidine biosynthesis; first step. [C]

COG id: COG0505

COG function: function code EF; Carbamoylphosphate synthase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Homo sapiens, GI18105007, Length=382, Percent_Identity=39.5287958115183, Blast_Score=233, Evalue=2e-61,
Organism=Homo sapiens, GI21361331, Length=407, Percent_Identity=34.1523341523341, Blast_Score=206, Evalue=2e-53,
Organism=Homo sapiens, GI169790915, Length=407, Percent_Identity=34.1523341523341, Blast_Score=206, Evalue=2e-53,
Organism=Escherichia coli, GI1786215, Length=384, Percent_Identity=54.6875, Blast_Score=404, Evalue=1e-114,
Organism=Caenorhabditis elegans, GI193204318, Length=386, Percent_Identity=35.7512953367876, Blast_Score=221, Evalue=4e-58,
Organism=Saccharomyces cerevisiae, GI6322331, Length=424, Percent_Identity=35.6132075471698, Blast_Score=229, Evalue=8e-61,
Organism=Saccharomyces cerevisiae, GI6324878, Length=389, Percent_Identity=37.0179948586118, Blast_Score=228, Evalue=9e-61,
Organism=Saccharomyces cerevisiae, GI6322638, Length=204, Percent_Identity=28.4313725490196, Blast_Score=65, Evalue=2e-11,
Organism=Drosophila melanogaster, GI45555749, Length=390, Percent_Identity=37.4358974358974, Blast_Score=216, Evalue=2e-56,
Organism=Drosophila melanogaster, GI24642586, Length=389, Percent_Identity=37.0179948586118, Blast_Score=216, Evalue=3e-56,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2599 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006220
- InterPro:   IPR001317
- InterPro:   IPR006274
- InterPro:   IPR002474
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991 [H]

Pfam domain/function: PF00988 CPSase_sm_chain; PF00117 GATase [H]

EC number: =6.3.5.5 [H]

Molecular weight: Translated: 42495; Mature: 42364

Theoretical pI: Translated: 5.87; Mature: 5.87

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASTAPWSETRPTALLVLADGTVIEGRGLGAAGSAVGEVCFNTALTGYQEILTDPSYAGQ
CCCCCCCCCCCCEEEEEEECCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCE
IVTFTFPHIGNVGTNDEDIEDLNPAARAGAVGAIFKADVTDPSNYRAGEHLDRWLKRRGI
EEEEECCCCCCCCCCCCCHHHCCHHHHCCCCEEEEEECCCCCCCCCCHHHHHHHHHHCCE
VALAGIDTRALTSLLREKGAANAVIAHAPDGRFDVEALKKEASEWGGLVGLDLAKVVTSG
EEEECCCHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHCCEEHHHHHHHHHCC
QSSTWTETPWVWSEGYAEARETPYHIVTVDYGVKRNILRMLSSLGAKVTVVPATASAEDI
CCCCCCCCCCCCCCCCHHHCCCCEEEEEECCCHHHHHHHHHHHCCCEEEEEECCCCCCCE
LAMRPDGVFLSNGPGDPAATGAYAVPMIRELLKTETPVFGICLGHQMLALALGARTVKMH
EEECCCCEEEECCCCCCCCCCCHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHCCCEEEEE
QGHHGANHPVKDFTTGKVEIVSMNHGFAVDSDSLPEGVEETHVSLFDGSNCGISLAGRPV
CCCCCCCCCCCCCCCCEEEEEEECCCEEECCCCCCCCHHHHEEEEEECCCCCEEECCCEE
FSVQHHPEASPGPQDSHYLFRRFINLVREKKGEPALAER
EEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
ASTAPWSETRPTALLVLADGTVIEGRGLGAAGSAVGEVCFNTALTGYQEILTDPSYAGQ
CCCCCCCCCCCEEEEEEECCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCE
IVTFTFPHIGNVGTNDEDIEDLNPAARAGAVGAIFKADVTDPSNYRAGEHLDRWLKRRGI
EEEEECCCCCCCCCCCCCHHHCCHHHHCCCCEEEEEECCCCCCCCCCHHHHHHHHHHCCE
VALAGIDTRALTSLLREKGAANAVIAHAPDGRFDVEALKKEASEWGGLVGLDLAKVVTSG
EEEECCCHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHCCEEHHHHHHHHHCC
QSSTWTETPWVWSEGYAEARETPYHIVTVDYGVKRNILRMLSSLGAKVTVVPATASAEDI
CCCCCCCCCCCCCCCCHHHCCCCEEEEEECCCHHHHHHHHHHHCCCEEEEEECCCCCCCE
LAMRPDGVFLSNGPGDPAATGAYAVPMIRELLKTETPVFGICLGHQMLALALGARTVKMH
EEECCCCEEEECCCCCCCCCCCHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHCCCEEEEE
QGHHGANHPVKDFTTGKVEIVSMNHGFAVDSDSLPEGVEETHVSLFDGSNCGISLAGRPV
CCCCCCCCCCCCCCCCEEEEEEECCCEEECCCCCCCCHHHHEEEEEECCCCCEEECCCEE
FSVQHHPEASPGPQDSHYLFRRFINLVREKKGEPALAER
EEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11214968 [H]