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The map label for this gene is nikA [C]

Identifier: 110634185

GI number: 110634185

Start: 1970669

End: 1972360

Strand: Reverse

Name: nikA [C]

Synonym: Meso_1835

Alternate gene names: 110634185

Gene position: 1972360-1970669 (Counterclockwise)

Preceding gene: 110634186

Following gene: 110634184

Centisome position: 44.7

GC content: 58.92

Gene sequence:

>1692_bases
ATGAACATCCGACTACATGGTCTAACAACAATTCTATCTGCATTGGTAGTGTTGACGGCGCCGACCGCTGTCCTTGCTCA
GGACAATTTGGTCACCGGGGAGTTGATCACGACCGTCAATTCGGGCACGCCCGGAAAGGGCGGTGAGGTTACCTTCGCAG
TCACGCGCGACATCACCAACTGGAACGTCACCTCGGCCCTCGGCAACAACGCCGTGGTCCGCACGGTAACGCTGCCAATC
GTTCCGTCGGCTTTCATGGTGCAGCCGGACTTCACCCTGAAGATGAACACCGACCTCCTGGAGAGCGCCGAGCTTACATC
GACCGATCCCCAAACCGTGGTCTACAGGATCAGGGAAGATGCGGTGTGGAGCGACGGCGTCCCGATAACGGGCGACGACT
TCATCTATTTCTGGAAGACGCAAAACCGCCGCGACTGCCCGGAATGCCTGATCAATGCGAGCTATGGCCACGACTTCATC
GAAACGCTCGAGCAGGACGAGACCGGAAAAGTCGTGACGGCGACATTCAGCGAGCCGTTCCTGGGCTGGCAAGGACTTTT
CATGTTCCTCTATCCGGCTCATCTAGCCGAGAAGCACGGCGACATCGCGGAGAGCTACAACAACTTCCTCTCCAATGAGG
TGCCGGCCTGGTCGGGCGGACCCTACATGGTCGAGTCGTTCGATCCGGGGCAACTCGTCACGCTTGTGCCAAATCCGAAA
TGGTATGGCGAAAAAGGGCCTTATCTGGACAAGCTGAAATTCCGGATCATCACCGATTCAACTCAGCAGCTGACGGCGCT
CGAAAACGGCGAGGTCGATGTGATCTATCCGCAGGGCGCCACCCAGGACATGGTCGAGCAGGCGGCGGGCCTCGATTATC
TCGGCATCGATTTCCAGATGAATCCGTCCGCCAACTGGTATTTCATGGGCCTCAACTCCAAAGCCGGCCCGATGTCCGAC
ATCGCGCTGCGCAAGGCGGTTCTGACGGCTATTGACGCCGGTGACCTGAAGGCCAAGACCGCTGATCCGTATTTGCGCAA
CTGGCCCCATATGGGCAGCGTGATGTTCCTGCCCAACCAGGCAGGTTATGCCGACCGCAGGGGAGCGCGCGGCTACGGTA
CGGGGGACGTGGAGAAGGCGAAGGGGATCCTCTCCGAGGCGGGCTACAAGCTCAGCGGAGGCAGTCTGCTGGATCCCTCC
GGCAAGCCTGTATCCACGCTTCGGCTTAGCTTTCCGCCCGGCTATCCGGCGGCCAACGACATGGCACGACTGATCACAGG
CTATATCGCCCCGCTCGGCCTCAAGACGGATTTGTTGACCGGCCCGAACGCCACCGCCGACTATCTCCTGAGCGGCAATT
TCGACCTTCACCTCAACTATTTCTCGCAGCAGGTCTTTCCCGCGGTGAAGGCGGGGCAGATTTTCCTCAGGGATACCCGT
CAGAACTATTTCGGCTTCAATGATCCGAAAATCGAGGAGATCATCGGCAAGGCTGCGGCAGCCTCGTCAATAGAGGAATC
TGCCGCAATCCTGTCCGAGGCCGACGAGCTCGCGATGGATTACGCGGCGCTATTCCCGATCTATCAGTTGCCGACGGCGC
TGATCTACAAGGAAGCGATCCTCAACCTGCGCGACAATCCGAACCAACTTGGTCCGGCCTACAACACGGCCGAATGGGGT
TTAGCGGAGTGA

Upstream 100 bases:

>100_bases
GTGATCCGGTATTGAGAAGTCGTACAACAAAAAATCTCAATTCGGGTCCGATTTGCATCGGGTTAATAGAAATATGTACT
CGTATGGATGGGAGTAATAC

Downstream 100 bases:

>100_bases
ATGGGCGGAAGGGGCCGGGCAGCTTCCGGCATGACGGCCCCTTCACCCAAATCGTTCCGCCGATGATTTTCCAAGCTGCA
GGGAGTTGAGCTTGCTAGCC

Product: extracellular solute-binding protein

Products: Ni2; [Cytoplasm]; ADP; phosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 563; Mature: 563

Protein sequence:

>563_residues
MNIRLHGLTTILSALVVLTAPTAVLAQDNLVTGELITTVNSGTPGKGGEVTFAVTRDITNWNVTSALGNNAVVRTVTLPI
VPSAFMVQPDFTLKMNTDLLESAELTSTDPQTVVYRIREDAVWSDGVPITGDDFIYFWKTQNRRDCPECLINASYGHDFI
ETLEQDETGKVVTATFSEPFLGWQGLFMFLYPAHLAEKHGDIAESYNNFLSNEVPAWSGGPYMVESFDPGQLVTLVPNPK
WYGEKGPYLDKLKFRIITDSTQQLTALENGEVDVIYPQGATQDMVEQAAGLDYLGIDFQMNPSANWYFMGLNSKAGPMSD
IALRKAVLTAIDAGDLKAKTADPYLRNWPHMGSVMFLPNQAGYADRRGARGYGTGDVEKAKGILSEAGYKLSGGSLLDPS
GKPVSTLRLSFPPGYPAANDMARLITGYIAPLGLKTDLLTGPNATADYLLSGNFDLHLNYFSQQVFPAVKAGQIFLRDTR
QNYFGFNDPKIEEIIGKAAAASSIEESAAILSEADELAMDYAALFPIYQLPTALIYKEAILNLRDNPNQLGPAYNTAEWG
LAE

Sequences:

>Translated_563_residues
MNIRLHGLTTILSALVVLTAPTAVLAQDNLVTGELITTVNSGTPGKGGEVTFAVTRDITNWNVTSALGNNAVVRTVTLPI
VPSAFMVQPDFTLKMNTDLLESAELTSTDPQTVVYRIREDAVWSDGVPITGDDFIYFWKTQNRRDCPECLINASYGHDFI
ETLEQDETGKVVTATFSEPFLGWQGLFMFLYPAHLAEKHGDIAESYNNFLSNEVPAWSGGPYMVESFDPGQLVTLVPNPK
WYGEKGPYLDKLKFRIITDSTQQLTALENGEVDVIYPQGATQDMVEQAAGLDYLGIDFQMNPSANWYFMGLNSKAGPMSD
IALRKAVLTAIDAGDLKAKTADPYLRNWPHMGSVMFLPNQAGYADRRGARGYGTGDVEKAKGILSEAGYKLSGGSLLDPS
GKPVSTLRLSFPPGYPAANDMARLITGYIAPLGLKTDLLTGPNATADYLLSGNFDLHLNYFSQQVFPAVKAGQIFLRDTR
QNYFGFNDPKIEEIIGKAAAASSIEESAAILSEADELAMDYAALFPIYQLPTALIYKEAILNLRDNPNQLGPAYNTAEWG
LAE
>Mature_563_residues
MNIRLHGLTTILSALVVLTAPTAVLAQDNLVTGELITTVNSGTPGKGGEVTFAVTRDITNWNVTSALGNNAVVRTVTLPI
VPSAFMVQPDFTLKMNTDLLESAELTSTDPQTVVYRIREDAVWSDGVPITGDDFIYFWKTQNRRDCPECLINASYGHDFI
ETLEQDETGKVVTATFSEPFLGWQGLFMFLYPAHLAEKHGDIAESYNNFLSNEVPAWSGGPYMVESFDPGQLVTLVPNPK
WYGEKGPYLDKLKFRIITDSTQQLTALENGEVDVIYPQGATQDMVEQAAGLDYLGIDFQMNPSANWYFMGLNSKAGPMSD
IALRKAVLTAIDAGDLKAKTADPYLRNWPHMGSVMFLPNQAGYADRRGARGYGTGDVEKAKGILSEAGYKLSGGSLLDPS
GKPVSTLRLSFPPGYPAANDMARLITGYIAPLGLKTDLLTGPNATADYLLSGNFDLHLNYFSQQVFPAVKAGQIFLRDTR
QNYFGFNDPKIEEIIGKAAAASSIEESAAILSEADELAMDYAALFPIYQLPTALIYKEAILNLRDNPNQLGPAYNTAEWG
LAE

Specific function: Involved In A Nickel Transport System, Probably Represents The Nickel Binder. [C]

COG id: COG0747

COG function: function code E; ABC-type dipeptide transport system, periplasmic component

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1789887, Length=458, Percent_Identity=22.4890829694323, Blast_Score=66, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 61444; Mature: 61444

Theoretical pI: Translated: 4.38; Mature: 4.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIRLHGLTTILSALVVLTAPTAVLAQDNLVTGELITTVNSGTPGKGGEVTFAVTRDITN
CCEEEHHHHHHHHHHHHHHCCCHHEECCCCEECCEEEEECCCCCCCCCEEEEEEEECCCC
WNVTSALGNNAVVRTVTLPIVPSAFMVQPDFTLKMNTDLLESAELTSTDPQTVVYRIRED
CEEHHHCCCCEEEEEEEEEECCCEEEECCCCEEEECCHHHCCCCCCCCCCCCEEEEEECC
AVWSDGVPITGDDFIYFWKTQNRRDCPECLINASYGHDFIETLEQDETGKVVTATFSEPF
CCCCCCCCCCCCCEEEEEECCCCCCCHHHHEECCCCCHHHHHHCCCCCCCEEEEECCCCC
LGWQGLFMFLYPAHLAEKHGDIAESYNNFLSNEVPAWSGGPYMVESFDPGQLVTLVPNPK
CCHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCEEEEECCCC
WYGEKGPYLDKLKFRIITDSTQQLTALENGEVDVIYPQGATQDMVEQAAGLDYLGIDFQM
CCCCCCCCCCEEEEEEEECCCHHEEEECCCCEEEEECCCCCHHHHHHHCCCCEEEEEEEE
NPSANWYFMGLNSKAGPMSDIALRKAVLTAIDAGDLKAKTADPYLRNWPHMGSVMFLPNQ
CCCCCEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHCCCCCCCEEECCCC
AGYADRRGARGYGTGDVEKAKGILSEAGYKLSGGSLLDPSGKPVSTLRLSFPPGYPAAND
CCCCCCCCCCCCCCCCHHHHHHHHHHCCCEECCCCEECCCCCCEEEEEEECCCCCCCHHH
MARLITGYIAPLGLKTDLLTGPNATADYLLSGNFDLHLNYFSQQVFPAVKAGQIFLRDTR
HHHHHHHHHCCCCCCEEECCCCCCCCCEEEECCEEEEEEHHHHHHHCHHHCCEEEEEECC
QNYFGFNDPKIEEIIGKAAAASSIEESAAILSEADELAMDYAALFPIYQLPTALIYKEAI
HHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LNLRDNPNQLGPAYNTAEWGLAE
HCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MNIRLHGLTTILSALVVLTAPTAVLAQDNLVTGELITTVNSGTPGKGGEVTFAVTRDITN
CCEEEHHHHHHHHHHHHHHCCCHHEECCCCEECCEEEEECCCCCCCCCEEEEEEEECCCC
WNVTSALGNNAVVRTVTLPIVPSAFMVQPDFTLKMNTDLLESAELTSTDPQTVVYRIRED
CEEHHHCCCCEEEEEEEEEECCCEEEECCCCEEEECCHHHCCCCCCCCCCCCEEEEEECC
AVWSDGVPITGDDFIYFWKTQNRRDCPECLINASYGHDFIETLEQDETGKVVTATFSEPF
CCCCCCCCCCCCCEEEEEECCCCCCCHHHHEECCCCCHHHHHHCCCCCCCEEEEECCCCC
LGWQGLFMFLYPAHLAEKHGDIAESYNNFLSNEVPAWSGGPYMVESFDPGQLVTLVPNPK
CCHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCEEEEECCCC
WYGEKGPYLDKLKFRIITDSTQQLTALENGEVDVIYPQGATQDMVEQAAGLDYLGIDFQM
CCCCCCCCCCEEEEEEEECCCHHEEEECCCCEEEEECCCCCHHHHHHHCCCCEEEEEEEE
NPSANWYFMGLNSKAGPMSDIALRKAVLTAIDAGDLKAKTADPYLRNWPHMGSVMFLPNQ
CCCCCEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHCCCCCCCEEECCCC
AGYADRRGARGYGTGDVEKAKGILSEAGYKLSGGSLLDPSGKPVSTLRLSFPPGYPAAND
CCCCCCCCCCCCCCCCHHHHHHHHHHCCCEECCCCEECCCCCCEEEEEEECCCCCCCHHH
MARLITGYIAPLGLKTDLLTGPNATADYLLSGNFDLHLNYFSQQVFPAVKAGQIFLRDTR
HHHHHHHHHCCCCCCEEECCCCCCCCCEEEECCEEEEEEHHHHHHHCHHHCCEEEEEECC
QNYFGFNDPKIEEIIGKAAAASSIEESAAILSEADELAMDYAALFPIYQLPTALIYKEAI
HHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LNLRDNPNQLGPAYNTAEWGLAE
HCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Ni2; [Periplasm]; ATP; H2O [C]

Specific reaction: Ni2+ [Periplasm] + ATP + H2O = Ni2+ [Cytoplasm] + ADP + phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]