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The map label for this gene is miaA

Identifier: 110634094

GI number: 110634094

Start: 1863319

End: 1864197

Strand: Reverse

Name: miaA

Synonym: Meso_1743

Alternate gene names: 110634094

Gene position: 1864197-1863319 (Counterclockwise)

Preceding gene: 110634097

Following gene: 110634093

Centisome position: 42.25

GC content: 61.43

Gene sequence:

>879_bases
ATGTTGAAAGGCGCCACCCTGATAGCCGGGCCTACGGCCAGCGGCAAGTCGGCGCTGGCGCTTCAAATGGCGCGGGAAAG
AGATGGCGTCATCGTGAACGCGGACTCGATGCAGGTTTACTCAGTATTGCATCTCCTGACCGCGCGGCCCGGCCCGGAGG
AACTCGCCATGGCGCCGCACCGGCTCTATGGTCACGTTCCTCCAAGCAAGCCTTATTCCACCGGCCGCTGGATCAGCGAT
GTGAGAGAGCTTATCGAGGCGGAAGCGCTCGCAAGGAGGGCGGTTATCTTTGTGGGCGGCACCGGGCTTTATTTTCGAGC
GCTGACCGAGGGGCTGTCGCCGATGCCGGAGATACCCGAGGCGGTGCGAATACGCTGGCGCAAGCGGCTGGCGGAAGAAG
GAGGAGAGGCGCTGCATCGGCTGCTGGGCGAAGCAGATCCCCTTGCCGCCAGCCGTATTCGCCCTTCCGATAGCCAGCGC
ATCGTGCGTGCTCTGGAAGTGGTGGAAGCCTCCGGCCGGCCGATCAGCTATTGGCAAGGGCAGGCTTCACATCCGCTGGT
TGATATGACCTCCTGCCGAAAGATCGTTCTGATGCCTGACAGGAACACGCTTGCGAGCAGGATCGAAAAGCGTTTCGATC
AGATGCTGCGGCACGGCGCCGTGGAAGAGGTGAAACAGCTGCTTGCGCTTGATCTGCCCCCTTCCATGCCGGCGATGAAG
GCGATCGGCGTACGCGAGATTTCAGCGGTATTGGCAGGTGAAATCAGCCTAGATGAGGCCGGTTCACGCGCCATCGCGGC
GACCAGGCAATACGCGAAGCGGCAAATAACGTGGTTTCGTCATCAACTCGGGCCGGATTGGGAGCATATGCCCGTTTGA

Upstream 100 bases:

>100_bases
CGCCATATTCGCCAGGGGCAGGCTCACGGCTGGTGACCGCGGGTTGGAGATGAGCGTTGCGACGAGGGCCATGAAAGAAT
CCGCCGAAAGTGGAAAACCG

Downstream 100 bases:

>100_bases
GATTGGCAGCCGGGTATCTCTCTGGTGGAAAAATTATGGAAAGCCACAATCTTCAGTTTTCAATTAATTTCATATAAGCC
CTCTCTGTGCCAACAACTTA

Product: tRNA delta(2)-isopentenylpyrophosphate transferase

Products: NA

Alternate protein names: Dimethylallyl diphosphate:tRNA dimethylallyltransferase; DMAPP:tRNA dimethylallyltransferase; DMATase; Isopentenyl-diphosphate:tRNA isopentenyltransferase; IPP transferase; IPPT; IPTase

Number of amino acids: Translated: 292; Mature: 292

Protein sequence:

>292_residues
MLKGATLIAGPTASGKSALALQMARERDGVIVNADSMQVYSVLHLLTARPGPEELAMAPHRLYGHVPPSKPYSTGRWISD
VRELIEAEALARRAVIFVGGTGLYFRALTEGLSPMPEIPEAVRIRWRKRLAEEGGEALHRLLGEADPLAASRIRPSDSQR
IVRALEVVEASGRPISYWQGQASHPLVDMTSCRKIVLMPDRNTLASRIEKRFDQMLRHGAVEEVKQLLALDLPPSMPAMK
AIGVREISAVLAGEISLDEAGSRAIAATRQYAKRQITWFRHQLGPDWEHMPV

Sequences:

>Translated_292_residues
MLKGATLIAGPTASGKSALALQMARERDGVIVNADSMQVYSVLHLLTARPGPEELAMAPHRLYGHVPPSKPYSTGRWISD
VRELIEAEALARRAVIFVGGTGLYFRALTEGLSPMPEIPEAVRIRWRKRLAEEGGEALHRLLGEADPLAASRIRPSDSQR
IVRALEVVEASGRPISYWQGQASHPLVDMTSCRKIVLMPDRNTLASRIEKRFDQMLRHGAVEEVKQLLALDLPPSMPAMK
AIGVREISAVLAGEISLDEAGSRAIAATRQYAKRQITWFRHQLGPDWEHMPV
>Mature_292_residues
MLKGATLIAGPTASGKSALALQMARERDGVIVNADSMQVYSVLHLLTARPGPEELAMAPHRLYGHVPPSKPYSTGRWISD
VRELIEAEALARRAVIFVGGTGLYFRALTEGLSPMPEIPEAVRIRWRKRLAEEGGEALHRLLGEADPLAASRIRPSDSQR
IVRALEVVEASGRPISYWQGQASHPLVDMTSCRKIVLMPDRNTLASRIEKRFDQMLRHGAVEEVKQLLALDLPPSMPAMK
AIGVREISAVLAGEISLDEAGSRAIAATRQYAKRQITWFRHQLGPDWEHMPV

Specific function: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A)

COG id: COG0324

COG function: function code J; tRNA delta(2)-isopentenylpyrophosphate transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IPP transferase family

Homologues:

Organism=Homo sapiens, GI31581534, Length=319, Percent_Identity=28.2131661442006, Blast_Score=99, Evalue=4e-21,
Organism=Escherichia coli, GI1790613, Length=288, Percent_Identity=42.0138888888889, Blast_Score=199, Evalue=2e-52,
Organism=Saccharomyces cerevisiae, GI6324848, Length=302, Percent_Identity=29.4701986754967, Blast_Score=123, Evalue=3e-29,
Organism=Drosophila melanogaster, GI24649830, Length=333, Percent_Identity=25.5255255255255, Blast_Score=88, Evalue=7e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAA_MESSB (Q11HI8)

Other databases:

- EMBL:   CP000390
- RefSeq:   YP_674302.1
- ProteinModelPortal:   Q11HI8
- SMR:   Q11HI8
- STRING:   Q11HI8
- GeneID:   4182506
- GenomeReviews:   CP000390_GR
- KEGG:   mes:Meso_1743
- NMPDR:   fig|266779.1.peg.3825
- eggNOG:   COG0324
- HOGENOM:   HBG559459
- OMA:   VNADSMQ
- PhylomeDB:   Q11HI8
- BioCyc:   MSP266779:MESO_1743-MONOMER
- HAMAP:   MF_00185
- InterPro:   IPR002627
- InterPro:   IPR018022
- PANTHER:   PTHR11088
- TIGRFAMs:   TIGR00174

Pfam domain/function: PF01715 IPPT

EC number: =2.5.1.75

Molecular weight: Translated: 32227; Mature: 32227

Theoretical pI: Translated: 9.84; Mature: 9.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKGATLIAGPTASGKSALALQMARERDGVIVNADSMQVYSVLHLLTARPGPEELAMAPH
CCCCCEEEECCCCCCCHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHCCCCHHHHHCCCH
RLYGHVPPSKPYSTGRWISDVRELIEAEALARRAVIFVGGTGLYFRALTEGLSPMPEIPE
HHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHEEEEECCCHHHHHHHHHCCCCCCCCCH
AVRIRWRKRLAEEGGEALHRLLGEADPLAASRIRPSDSQRIVRALEVVEASGRPISYWQG
HHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCCHHHHHHHHHHHHHCCCCCCEECCC
QASHPLVDMTSCRKIVLMPDRNTLASRIEKRFDQMLRHGAVEEVKQLLALDLPPSMPAMK
CCCCCCEECCCCCEEEEECCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCHHH
AIGVREISAVLAGEISLDEAGSRAIAATRQYAKRQITWFRHQLGPDWEHMPV
HHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MLKGATLIAGPTASGKSALALQMARERDGVIVNADSMQVYSVLHLLTARPGPEELAMAPH
CCCCCEEEECCCCCCCHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHCCCCHHHHHCCCH
RLYGHVPPSKPYSTGRWISDVRELIEAEALARRAVIFVGGTGLYFRALTEGLSPMPEIPE
HHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHEEEEECCCHHHHHHHHHCCCCCCCCCH
AVRIRWRKRLAEEGGEALHRLLGEADPLAASRIRPSDSQRIVRALEVVEASGRPISYWQG
HHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCCHHHHHHHHHHHHHCCCCCCEECCC
QASHPLVDMTSCRKIVLMPDRNTLASRIEKRFDQMLRHGAVEEVKQLLALDLPPSMPAMK
CCCCCCEECCCCCEEEEECCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCHHH
AIGVREISAVLAGEISLDEAGSRAIAATRQYAKRQITWFRHQLGPDWEHMPV
HHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA