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The map label for this gene is gltX

Identifier: 110633997

GI number: 110633997

Start: 1773324

End: 1774751

Strand: Direct

Name: gltX

Synonym: Meso_1645

Alternate gene names: 110633997

Gene position: 1773324-1774751 (Clockwise)

Preceding gene: 110633994

Following gene: 110633998

Centisome position: 40.19

GC content: 62.18

Gene sequence:

>1428_bases
ATGAAAACGCCCGTCATCACTCGCTTTGCGCCCTCGCCCACCGGCTTTCTTCATATAGGCGGCGCCCGCACGGCCCTTTT
CAATTGGCTCTATGCACGCCATACCGGGGGCAGGATGCTGTTGAGGATCGAAGACACGGACCGGCAACGCTCCACACCGG
AGGCAACCGCCGCGATCCTGGACGGGCTTGCCTGGCTGGGGCTTACCTGGGACGGAGAGCCGATCTCCCAGTTTGCGCGG
GCGAGCCGTCATAAGGAGGTGGCCGAGGAACTCGTCGCCCGAGGGCAGGCCTATTATTGCTACTGCTCACCGGAGGAAAT
CGAGGCCATGCGCGAAAAGGCGCGCGCCGAAGGACGGCCGCCGCGCTACGACGGCACATGGCGTGACAAGGACCCGGCCG
AAGCCCCAAGCGGGGTGAAACCGGTGATCCGCATCAAGGCGCCGCTGGAGGGCGAGACGGTCATTCACGATCGCGTGCAG
GGCGACGTCCGCTTCCCCAACAAGGATTTGGACGATTTCATCATCCTGCGCTCCGATGGCACGCCGACCTACATGCATGC
GGTGGTCGTGGACGATCACGACATGGGGGTCACCCATGTCATTCGCGGCGATGACCATCTCACCAATGCGGCGCGCCAGG
CAATCATCTACCGGGCAATGGGCTGGGACATTCCGGTAATGGCTCATATCCCGCTGATCCACGGGCCTGACGGCGGAAAG
CTCTCGAAGCGCCATGGTGCGCTTGGCGTCGAAGCCTACCGGGCGATGGGCTACCTCCCCTCGGCCCTATGCAACTATCT
CGCGCGTCTTGGCTGGAGCCACGGCGATGATGAAGTGATGACGACCGAGCAGATGATCGAATGGTTCGATATCGACGACG
TGAACAAGGGGGCCGCGCGCTTCGACTTCCAGAAAATGGAGGCGCTCAACGGCATCCATATGCGCCAAATGGATGATGAG
GCGCTGTTCGGAATTTTTATTGCGACGCTTCCTCACCTGGAGGGAGGGCAAGCCTTTCTGGATAAGCTGGACGAGAAGAG
GCGTGGTCAACTTCGCTCGGCCCTTCCCGGCCTGAAAGAAAGAGCCAAGACCCTGTTGGAGCTGTTGGATGGCGCGGGCT
TTCTTGTTGCCGAGCGTCCGCTTACCATCGACGATAAGGCGGCCGAACTGCTCGACGGTGATGCTCGAGCCATGCTCGCC
ACCCTTGCCGAAGAAATGGCAGGGCTTGGCGACTGGACGGCTGAAAGCACCGAAGCGGCCGTGCGCAATTTTGCGGAGAA
TACCGGCCGCAAGCTGGGCAAGGTGGCCCAGCCGCTGCGGGCGGCGCTCACCGGCCGCACCACTTCGCCCGGCATCTTCG
ATGTTCTGGCGGTACTCGGGCGCGAAGAATCGCTCGGCCGCATTCGCGACCAAGCGGTTGAAAAATGA

Upstream 100 bases:

>100_bases
CGCTTGCGCCTGACCTTGCCTATGCTACATGACGGGCAGCCGATTTCCACCCGCGCCGGCTGTGGCATAAGGCACAGCTT
GAACATATCCGGAAAGAACA

Downstream 100 bases:

>100_bases
GGTCCAGCAAACTGGATATGCTGCGGCGCAACAAAGTGGCCCTGTCAAGCTTGGCAGGGTGTGGCAAATGCGCTAGCTAG
AGGCACCTTAAAGCCGTGCT

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase 2; GluRS 2

Number of amino acids: Translated: 475; Mature: 475

Protein sequence:

>475_residues
MKTPVITRFAPSPTGFLHIGGARTALFNWLYARHTGGRMLLRIEDTDRQRSTPEATAAILDGLAWLGLTWDGEPISQFAR
ASRHKEVAEELVARGQAYYCYCSPEEIEAMREKARAEGRPPRYDGTWRDKDPAEAPSGVKPVIRIKAPLEGETVIHDRVQ
GDVRFPNKDLDDFIILRSDGTPTYMHAVVVDDHDMGVTHVIRGDDHLTNAARQAIIYRAMGWDIPVMAHIPLIHGPDGGK
LSKRHGALGVEAYRAMGYLPSALCNYLARLGWSHGDDEVMTTEQMIEWFDIDDVNKGAARFDFQKMEALNGIHMRQMDDE
ALFGIFIATLPHLEGGQAFLDKLDEKRRGQLRSALPGLKERAKTLLELLDGAGFLVAERPLTIDDKAAELLDGDARAMLA
TLAEEMAGLGDWTAESTEAAVRNFAENTGRKLGKVAQPLRAALTGRTTSPGIFDVLAVLGREESLGRIRDQAVEK

Sequences:

>Translated_475_residues
MKTPVITRFAPSPTGFLHIGGARTALFNWLYARHTGGRMLLRIEDTDRQRSTPEATAAILDGLAWLGLTWDGEPISQFAR
ASRHKEVAEELVARGQAYYCYCSPEEIEAMREKARAEGRPPRYDGTWRDKDPAEAPSGVKPVIRIKAPLEGETVIHDRVQ
GDVRFPNKDLDDFIILRSDGTPTYMHAVVVDDHDMGVTHVIRGDDHLTNAARQAIIYRAMGWDIPVMAHIPLIHGPDGGK
LSKRHGALGVEAYRAMGYLPSALCNYLARLGWSHGDDEVMTTEQMIEWFDIDDVNKGAARFDFQKMEALNGIHMRQMDDE
ALFGIFIATLPHLEGGQAFLDKLDEKRRGQLRSALPGLKERAKTLLELLDGAGFLVAERPLTIDDKAAELLDGDARAMLA
TLAEEMAGLGDWTAESTEAAVRNFAENTGRKLGKVAQPLRAALTGRTTSPGIFDVLAVLGREESLGRIRDQAVEK
>Mature_475_residues
MKTPVITRFAPSPTGFLHIGGARTALFNWLYARHTGGRMLLRIEDTDRQRSTPEATAAILDGLAWLGLTWDGEPISQFAR
ASRHKEVAEELVARGQAYYCYCSPEEIEAMREKARAEGRPPRYDGTWRDKDPAEAPSGVKPVIRIKAPLEGETVIHDRVQ
GDVRFPNKDLDDFIILRSDGTPTYMHAVVVDDHDMGVTHVIRGDDHLTNAARQAIIYRAMGWDIPVMAHIPLIHGPDGGK
LSKRHGALGVEAYRAMGYLPSALCNYLARLGWSHGDDEVMTTEQMIEWFDIDDVNKGAARFDFQKMEALNGIHMRQMDDE
ALFGIFIATLPHLEGGQAFLDKLDEKRRGQLRSALPGLKERAKTLLELLDGAGFLVAERPLTIDDKAAELLDGDARAMLA
TLAEEMAGLGDWTAESTEAAVRNFAENTGRKLGKVAQPLRAALTGRTTSPGIFDVLAVLGREESLGRIRDQAVEK

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu)

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI134288884, Length=489, Percent_Identity=31.6973415132924, Blast_Score=228, Evalue=1e-59,
Organism=Homo sapiens, GI4826960, Length=107, Percent_Identity=37.3831775700935, Blast_Score=68, Evalue=2e-11,
Organism=Escherichia coli, GI1788743, Length=465, Percent_Identity=44.5161290322581, Blast_Score=391, Evalue=1e-110,
Organism=Escherichia coli, GI226510925, Length=286, Percent_Identity=36.3636363636364, Blast_Score=146, Evalue=3e-36,
Organism=Escherichia coli, GI1786895, Length=132, Percent_Identity=31.0606060606061, Blast_Score=68, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI71993394, Length=495, Percent_Identity=31.1111111111111, Blast_Score=199, Evalue=3e-51,
Organism=Saccharomyces cerevisiae, GI6324540, Length=488, Percent_Identity=31.3524590163934, Blast_Score=236, Evalue=4e-63,
Organism=Saccharomyces cerevisiae, GI6324742, Length=185, Percent_Identity=29.7297297297297, Blast_Score=81, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6321192, Length=215, Percent_Identity=29.7674418604651, Blast_Score=73, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24665321, Length=505, Percent_Identity=31.6831683168317, Blast_Score=248, Evalue=1e-65,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYE2_MESSB (Q11HT5)

Other databases:

- EMBL:   CP000390
- RefSeq:   YP_674205.1
- STRING:   Q11HT5
- GeneID:   4179244
- GenomeReviews:   CP000390_GR
- KEGG:   mes:Meso_1645
- NMPDR:   fig|266779.1.peg.391
- eggNOG:   COG0008
- HOGENOM:   HBG628189
- OMA:   MAHIPLI
- PhylomeDB:   Q11HT5
- ProtClustDB:   PRK01406
- BioCyc:   MSP266779:MESO_1645-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00022_B
- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729
- Gene3D:   G3DSA:1.10.1160.10
- Gene3D:   G3DSA:3.40.50.620
- Gene3D:   G3DSA:1.10.10.350
- PANTHER:   PTHR10119
- PRINTS:   PR00987
- TIGRFAMs:   TIGR00464

Pfam domain/function: PF00749 tRNA-synt_1c; SSF48163 tRNA-synt_bind

EC number: =6.1.1.17

Molecular weight: Translated: 52542; Mature: 52542

Theoretical pI: Translated: 5.77; Mature: 5.77

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 243-243

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTPVITRFAPSPTGFLHIGGARTALFNWLYARHTGGRMLLRIEDTDRQRSTPEATAAIL
CCCCCEEEECCCCCCEEEECCHHHHHHHHHHHEECCCEEEEEEECCCCCCCCHHHHHHHH
DGLAWLGLTWDGEPISQFARASRHKEVAEELVARGQAYYCYCSPEEIEAMREKARAEGRP
HHHHHEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEECHHHHHHHHHHHHHCCCC
PRYDGTWRDKDPAEAPSGVKPVIRIKAPLEGETVIHDRVQGDVRFPNKDLDDFIILRSDG
CCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCEEEHHHCCCCCCCCCCCCCCEEEEECCC
TPTYMHAVVVDDHDMGVTHVIRGDDHLTNAARQAIIYRAMGWDIPVMAHIPLIHGPDGGK
CCEEEEEEEEECCCCCEEEEECCCHHHHHHHHHHHHHHHCCCCCCEEEECCEEECCCCCC
LSKRHGALGVEAYRAMGYLPSALCNYLARLGWSHGDDEVMTTEQMIEWFDIDDVNKGAAR
CCHHCCCCHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCCCHHH
FDFQKMEALNGIHMRQMDDEALFGIFIATLPHLEGGQAFLDKLDEKRRGQLRSALPGLKE
HHHHHHHHHCCCHHHHCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHH
RAKTLLELLDGAGFLVAERPLTIDDKAAELLDGDARAMLATLAEEMAGLGDWTAESTEAA
HHHHHHHHHCCCCEEEECCCCCCCHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCHHHHHH
VRNFAENTGRKLGKVAQPLRAALTGRTTSPGIFDVLAVLGREESLGRIRDQAVEK
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCHHHHHHHHHHCCC
>Mature Secondary Structure
MKTPVITRFAPSPTGFLHIGGARTALFNWLYARHTGGRMLLRIEDTDRQRSTPEATAAIL
CCCCCEEEECCCCCCEEEECCHHHHHHHHHHHEECCCEEEEEEECCCCCCCCHHHHHHHH
DGLAWLGLTWDGEPISQFARASRHKEVAEELVARGQAYYCYCSPEEIEAMREKARAEGRP
HHHHHEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEECHHHHHHHHHHHHHCCCC
PRYDGTWRDKDPAEAPSGVKPVIRIKAPLEGETVIHDRVQGDVRFPNKDLDDFIILRSDG
CCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCEEEHHHCCCCCCCCCCCCCCEEEEECCC
TPTYMHAVVVDDHDMGVTHVIRGDDHLTNAARQAIIYRAMGWDIPVMAHIPLIHGPDGGK
CCEEEEEEEEECCCCCEEEEECCCHHHHHHHHHHHHHHHCCCCCCEEEECCEEECCCCCC
LSKRHGALGVEAYRAMGYLPSALCNYLARLGWSHGDDEVMTTEQMIEWFDIDDVNKGAAR
CCHHCCCCHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCCCHHH
FDFQKMEALNGIHMRQMDDEALFGIFIATLPHLEGGQAFLDKLDEKRRGQLRSALPGLKE
HHHHHHHHHCCCHHHHCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHH
RAKTLLELLDGAGFLVAERPLTIDDKAAELLDGDARAMLATLAEEMAGLGDWTAESTEAA
HHHHHHHHHCCCCEEEECCCCCCCHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCHHHHHH
VRNFAENTGRKLGKVAQPLRAALTGRTTSPGIFDVLAVLGREESLGRIRDQAVEK
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA