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The map label for this gene is ntrB [H]

Identifier: 110633971

GI number: 110633971

Start: 1745026

End: 1746159

Strand: Reverse

Name: ntrB [H]

Synonym: Meso_1619

Alternate gene names: 110633971

Gene position: 1746159-1745026 (Counterclockwise)

Preceding gene: 110633972

Following gene: 110633970

Centisome position: 39.57

GC content: 59.35

Gene sequence:

>1134_bases
ATGAACGCTAAAACGTCCGGCGTGGCGCAACTCGCCATCGATTCCATCGGTCACCCGGTGGTGATGGTAGACGAGAACGG
CTTTTTCACCTTCGCCAATGCGGAGGCGGAGAATTTCTTTCGGCTGAGCGCCACGATGCTTGCGCGCCACCAACTTGCCG
ATTTCGTACCGTTCGGCAGCCCGCTGCTGACGCTCGTCGAGCAAGTGCGCGAGCGACGCGCGCCCTTCAATGAATATCGC
GTCGAAATCTCCTCGCCCCGTCTCGGACCGGACAAGATCGTCGATATCTATGTGGCGCCCGTTTCAGAGCTTCCAGGCTC
GGCAGTCATCATGTTCCAGGAACGGTCAATGGCGGAAAAGATCGACCGGCAGATGACCCATCGGGGCGCTGCCCGGTCCG
TCACAGGGCTTGCCGCCATGCTGGCCCATGAAATCAAAAACCCACTTTCCGGGATACGCGGTGCGGCGCAGCTCCTCGAA
CATGTGGTTTCAGATGAGGACCGTGCGCTGGCACGCCTGATCACCGAGGAGACGGACAGGATCGTCGCGCTTGTCGATCG
CATGGAGGAGTTTTCTGACGAGCGTCCCGTCGACCGCGAGCCGGTGAATATCCACGTCGTGCTTGATCATGTGAAGGCGA
TCGCCAAGAACGGCTTCGCCAGGCACATCAACATAATCGAAGAGTACGACCCCTCGCTTCCGCCCATTTTTGCCAATAGG
GGCCAACTTGTTCAGGTCTTCTTAAACCTCATCAAGAATGCCTCGGAAGCTATTGGAAACGCTTCAACTGGCGAAATCAA
GCTCTCCACTGCCTTCCGGCCCGGCATCCGCTTTTCCGTGCCCGGAACGCGTGAACGGGTCTCCCTGCCGATGGAATTCT
GCGTCCATGACAACGGTCCGGGCGTCCCCGAAGACATTCAGGCGATTATTTTCGACCCGTTCATCACCACGAAGCCGAAC
GGCTCCGGGCTGGGATTGGCGTTGGTCGCAAAAATCGTCGGAGCGCATGGCGGCGTGATCGAATGCGATTCGTCCTCACG
CGGAACGACTTTCCGTGTGCTCATGCCGGCGTGGCGTGGCGATCCGCCGTCGTTGGTGGCGGGCCGGCGGAAGAAAACGG
ATGCGAAGGCATGA

Upstream 100 bases:

>100_bases
CTTTGCGGCGCTCGATTATGACGGAAACGGACCCTTCTAAGGTGCTCCACCTTATTGGCGGGATATTTTCTCAACCGGGT
CTCGTGCAAGGGCTAGCCGC

Downstream 100 bases:

>100_bases
GCGCACAAGGCAACATTCTGGTGGCCGATGACGACGCGGCCATTCGCACGGTCCTCAATCAGGCGCTGTCGCGCGTGGGG
CACCAGGTCCGCGTGACGTC

Product: signal transduction histidine kinase, nitrogen specific, NtrB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 377; Mature: 377

Protein sequence:

>377_residues
MNAKTSGVAQLAIDSIGHPVVMVDENGFFTFANAEAENFFRLSATMLARHQLADFVPFGSPLLTLVEQVRERRAPFNEYR
VEISSPRLGPDKIVDIYVAPVSELPGSAVIMFQERSMAEKIDRQMTHRGAARSVTGLAAMLAHEIKNPLSGIRGAAQLLE
HVVSDEDRALARLITEETDRIVALVDRMEEFSDERPVDREPVNIHVVLDHVKAIAKNGFARHINIIEEYDPSLPPIFANR
GQLVQVFLNLIKNASEAIGNASTGEIKLSTAFRPGIRFSVPGTRERVSLPMEFCVHDNGPGVPEDIQAIIFDPFITTKPN
GSGLGLALVAKIVGAHGGVIECDSSSRGTTFRVLMPAWRGDPPSLVAGRRKKTDAKA

Sequences:

>Translated_377_residues
MNAKTSGVAQLAIDSIGHPVVMVDENGFFTFANAEAENFFRLSATMLARHQLADFVPFGSPLLTLVEQVRERRAPFNEYR
VEISSPRLGPDKIVDIYVAPVSELPGSAVIMFQERSMAEKIDRQMTHRGAARSVTGLAAMLAHEIKNPLSGIRGAAQLLE
HVVSDEDRALARLITEETDRIVALVDRMEEFSDERPVDREPVNIHVVLDHVKAIAKNGFARHINIIEEYDPSLPPIFANR
GQLVQVFLNLIKNASEAIGNASTGEIKLSTAFRPGIRFSVPGTRERVSLPMEFCVHDNGPGVPEDIQAIIFDPFITTKPN
GSGLGLALVAKIVGAHGGVIECDSSSRGTTFRVLMPAWRGDPPSLVAGRRKKTDAKA
>Mature_377_residues
MNAKTSGVAQLAIDSIGHPVVMVDENGFFTFANAEAENFFRLSATMLARHQLADFVPFGSPLLTLVEQVRERRAPFNEYR
VEISSPRLGPDKIVDIYVAPVSELPGSAVIMFQERSMAEKIDRQMTHRGAARSVTGLAAMLAHEIKNPLSGIRGAAQLLE
HVVSDEDRALARLITEETDRIVALVDRMEEFSDERPVDREPVNIHVVLDHVKAIAKNGFARHINIIEEYDPSLPPIFANR
GQLVQVFLNLIKNASEAIGNASTGEIKLSTAFRPGIRFSVPGTRERVSLPMEFCVHDNGPGVPEDIQAIIFDPFITTKPN
GSGLGLALVAKIVGAHGGVIECDSSSRGTTFRVLMPAWRGDPPSLVAGRRKKTDAKA

Specific function: NtrB acts as a signal transducer which responds to the nitrogen level of cell and modulates the activity of ntrC. In nitrogen limitation ntrB activates ntrC by phosphorylating it, while in nitrogen excess ntrC is dephosphorylated and consequently inactiva

COG id: COG3852

COG function: function code T; Signal transduction histidine kinase, nitrogen specific

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1790300, Length=356, Percent_Identity=30.6179775280899, Blast_Score=147, Evalue=9e-37,
Organism=Escherichia coli, GI1788549, Length=370, Percent_Identity=29.7297297297297, Blast_Score=137, Evalue=1e-33,
Organism=Escherichia coli, GI1790436, Length=232, Percent_Identity=32.7586206896552, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1789808, Length=233, Percent_Identity=27.4678111587983, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1786912, Length=257, Percent_Identity=28.0155642023346, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI1788393, Length=229, Percent_Identity=25.764192139738, Blast_Score=62, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 41188; Mature: 41188

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAKTSGVAQLAIDSIGHPVVMVDENGFFTFANAEAENFFRLSATMLARHQLADFVPFGS
CCCCCHHHHHHHHHHCCCCEEEEECCCEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCC
PLLTLVEQVRERRAPFNEYRVEISSPRLGPDKIVDIYVAPVSELPGSAVIMFQERSMAEK
HHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCEEEEEEECHHHCCCCEEEEEECHHHHHH
IDRQMTHRGAARSVTGLAAMLAHEIKNPLSGIRGAAQLLEHVVSDEDRALARLITEETDR
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
IVALVDRMEEFSDERPVDREPVNIHVVLDHVKAIAKNGFARHINIIEEYDPSLPPIFANR
HHHHHHHHHHHCCCCCCCCCCCEEEEHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCC
GQLVQVFLNLIKNASEAIGNASTGEIKLSTAFRPGIRFSVPGTRERVSLPMEFCVHDNGP
CHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCEEEECCCCHHHHCCCHHEEEECCCC
GVPEDIQAIIFDPFITTKPNGSGLGLALVAKIVGAHGGVIECDSSSRGTTFRVLMPAWRG
CCCHHHHHEEECCCEECCCCCCCHHHHHHHHHHCCCCCEEEECCCCCCCEEEEEECCCCC
DPPSLVAGRRKKTDAKA
CCCHHHCCCCCCCCCCC
>Mature Secondary Structure
MNAKTSGVAQLAIDSIGHPVVMVDENGFFTFANAEAENFFRLSATMLARHQLADFVPFGS
CCCCCHHHHHHHHHHCCCCEEEEECCCEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCC
PLLTLVEQVRERRAPFNEYRVEISSPRLGPDKIVDIYVAPVSELPGSAVIMFQERSMAEK
HHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCEEEEEEECHHHCCCCEEEEEECHHHHHH
IDRQMTHRGAARSVTGLAAMLAHEIKNPLSGIRGAAQLLEHVVSDEDRALARLITEETDR
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
IVALVDRMEEFSDERPVDREPVNIHVVLDHVKAIAKNGFARHINIIEEYDPSLPPIFANR
HHHHHHHHHHHCCCCCCCCCCCEEEEHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCC
GQLVQVFLNLIKNASEAIGNASTGEIKLSTAFRPGIRFSVPGTRERVSLPMEFCVHDNGP
CHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCEEEECCCCHHHHCCCHHEEEECCCC
GVPEDIQAIIFDPFITTKPNGSGLGLALVAKIVGAHGGVIECDSSSRGTTFRVLMPAWRG
CCCHHHHHEEECCCEECCCCCCCHHHHHHHHHHCCCCCEEEECCCCCCCEEEEEECCCCC
DPPSLVAGRRKKTDAKA
CCCHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11481430; 2881918 [H]