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The map label for this gene is dus [H]

Identifier: 110633972

GI number: 110633972

Start: 1746156

End: 1747220

Strand: Reverse

Name: dus [H]

Synonym: Meso_1620

Alternate gene names: 110633972

Gene position: 1747220-1746156 (Counterclockwise)

Preceding gene: 110633975

Following gene: 110633971

Centisome position: 39.6

GC content: 63.0

Gene sequence:

>1065_bases
ATGTCTAACAAATGGGCATTCAGCAAGGATGCCTTATTCTTGAGCAACAGTGCGCTGCAAATCCTTTCCCAACCGCTTCA
GGTCGGCGCGGTCCGGCTGCGGAACCGCGTGTTCCTGGCGCCTATGTCCGGTGTGACGGATCTGCCGTTTCGCATGCGCG
CTTATGCGCATGGCGCCGGGTTGGTCGTATCCGAGATGGTGGCCAGCGGTGAGCTGGCGAAGGGCAGGGCGGAGAGTGCA
AGGCGCATCCGCCGGCCCGATATCGACGTCCACATGGTTCAGATCGCCGGTCGCGAAGCGCGCTGGATGGCCGAGGCGGC
GCGCATCGCCGTGGACGAGGGTGCGGACATCGTCGACATCAATATGGGCTGTCCGGCCAAGAAAGTGACGGGCGGCTATT
CCGGCTCCGCTCTCATGCGCGAGCCCGATCACGCACTGTCGCTGATCGAGGCGGTGGTAAAAGCCGTGGATGTGCCGGTG
ACAGTCAAGATGCGGCTTGGCTGGGATGATGCGTGCCTCAACGCGCCCGAGCTTGCTCGCCGGGCTGAAGCCGCCGGCGT
GGCGATGGTGACGGTTCATGGGCGCACGCGCTGCCAGTTCTATACCGGTCAGGCAAATTGGAAAGCGATAGCGGAGGTCA
AGCAGGCGGTCTCCATCCCGGTCGTCGCCAATGGGGATGGCGAAGGACCGTTGGAGGCTGCGACTATGCTCACCCAATCA
GGCGCTGACGCCGTGATGATCGGCCGGGCGCATTACGGCGCGCCGTGGAAGGCCGGCGCGCTGGCGCATGCCGCAGACGG
CAGTGCGGCGCCTGGCGTACCGGCCGGTACGGCCGAGATGGCAGATTATGTTGCGGCGCATTACGTGGATATGCTTTCGC
ATTACGGCACGGGGCAGGGAACGCGCCACGCAAGAAAGCATATCGGATGGTATCTGGACCGTCATTCAGCCCCTGATCCC
GCTTTGCGGCGCTCGATTATGACGGAAACGGACCCTTCTAAGGTGCTCCACCTTATTGGCGGGATATTTTCTCAACCGGG
TCTCGTGCAAGGGCTAGCCGCATGA

Upstream 100 bases:

>100_bases
CAAAAGGTGCGTTCATGCCGAAGACGCATAATTGCGGCAAGGTTGAAAAGCTACCGGATTCTCTGCCATTACAACGCAGC
TATTGCGCAACACGCACGAA

Downstream 100 bases:

>100_bases
ACGCTAAAACGTCCGGCGTGGCGCAACTCGCCATCGATTCCATCGGTCACCCGGTGGTGATGGTAGACGAGAACGGCTTT
TTCACCTTCGCCAATGCGGA

Product: nifR3 family TIM-barrel protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 354; Mature: 353

Protein sequence:

>354_residues
MSNKWAFSKDALFLSNSALQILSQPLQVGAVRLRNRVFLAPMSGVTDLPFRMRAYAHGAGLVVSEMVASGELAKGRAESA
RRIRRPDIDVHMVQIAGREARWMAEAARIAVDEGADIVDINMGCPAKKVTGGYSGSALMREPDHALSLIEAVVKAVDVPV
TVKMRLGWDDACLNAPELARRAEAAGVAMVTVHGRTRCQFYTGQANWKAIAEVKQAVSIPVVANGDGEGPLEAATMLTQS
GADAVMIGRAHYGAPWKAGALAHAADGSAAPGVPAGTAEMADYVAAHYVDMLSHYGTGQGTRHARKHIGWYLDRHSAPDP
ALRRSIMTETDPSKVLHLIGGIFSQPGLVQGLAA

Sequences:

>Translated_354_residues
MSNKWAFSKDALFLSNSALQILSQPLQVGAVRLRNRVFLAPMSGVTDLPFRMRAYAHGAGLVVSEMVASGELAKGRAESA
RRIRRPDIDVHMVQIAGREARWMAEAARIAVDEGADIVDINMGCPAKKVTGGYSGSALMREPDHALSLIEAVVKAVDVPV
TVKMRLGWDDACLNAPELARRAEAAGVAMVTVHGRTRCQFYTGQANWKAIAEVKQAVSIPVVANGDGEGPLEAATMLTQS
GADAVMIGRAHYGAPWKAGALAHAADGSAAPGVPAGTAEMADYVAAHYVDMLSHYGTGQGTRHARKHIGWYLDRHSAPDP
ALRRSIMTETDPSKVLHLIGGIFSQPGLVQGLAA
>Mature_353_residues
SNKWAFSKDALFLSNSALQILSQPLQVGAVRLRNRVFLAPMSGVTDLPFRMRAYAHGAGLVVSEMVASGELAKGRAESAR
RIRRPDIDVHMVQIAGREARWMAEAARIAVDEGADIVDINMGCPAKKVTGGYSGSALMREPDHALSLIEAVVKAVDVPVT
VKMRLGWDDACLNAPELARRAEAAGVAMVTVHGRTRCQFYTGQANWKAIAEVKQAVSIPVVANGDGEGPLEAATMLTQSG
ADAVMIGRAHYGAPWKAGALAHAADGSAAPGVPAGTAEMADYVAAHYVDMLSHYGTGQGTRHARKHIGWYLDRHSAPDPA
LRRSIMTETDPSKVLHLIGGIFSQPGLVQGLAA

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family [H]

Homologues:

Organism=Homo sapiens, GI239788483, Length=280, Percent_Identity=30, Blast_Score=117, Evalue=1e-26,
Organism=Homo sapiens, GI239788462, Length=293, Percent_Identity=29.3515358361775, Blast_Score=117, Evalue=1e-26,
Organism=Homo sapiens, GI31742496, Length=280, Percent_Identity=28.5714285714286, Blast_Score=99, Evalue=7e-21,
Organism=Homo sapiens, GI40807366, Length=231, Percent_Identity=30.3030303030303, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI8923374, Length=238, Percent_Identity=27.7310924369748, Blast_Score=87, Evalue=2e-17,
Organism=Escherichia coli, GI1789660, Length=324, Percent_Identity=45.3703703703704, Blast_Score=265, Evalue=2e-72,
Organism=Escherichia coli, GI1788462, Length=231, Percent_Identity=35.4978354978355, Blast_Score=119, Evalue=2e-28,
Organism=Escherichia coli, GI145693211, Length=258, Percent_Identity=27.1317829457364, Blast_Score=67, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17543114, Length=242, Percent_Identity=31.404958677686, Blast_Score=108, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI17507177, Length=217, Percent_Identity=32.7188940092166, Blast_Score=89, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI25144369, Length=203, Percent_Identity=32.0197044334975, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17510279, Length=246, Percent_Identity=28.4552845528455, Blast_Score=76, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6323560, Length=225, Percent_Identity=31.5555555555556, Blast_Score=96, Evalue=8e-21,
Organism=Saccharomyces cerevisiae, GI6323437, Length=275, Percent_Identity=24.7272727272727, Blast_Score=73, Evalue=5e-14,
Organism=Saccharomyces cerevisiae, GI6323433, Length=309, Percent_Identity=24.2718446601942, Blast_Score=72, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24585320, Length=240, Percent_Identity=35, Blast_Score=110, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24580595, Length=225, Percent_Identity=33.7777777777778, Blast_Score=108, Evalue=7e-24,
Organism=Drosophila melanogaster, GI19920448, Length=225, Percent_Identity=33.7777777777778, Blast_Score=108, Evalue=7e-24,
Organism=Drosophila melanogaster, GI45549423, Length=247, Percent_Identity=31.1740890688259, Blast_Score=96, Evalue=3e-20,
Organism=Drosophila melanogaster, GI19921524, Length=239, Percent_Identity=28.8702928870293, Blast_Score=94, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004652
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 37575; Mature: 37443

Theoretical pI: Translated: 8.78; Mature: 8.78

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNKWAFSKDALFLSNSALQILSQPLQVGAVRLRNRVFLAPMSGVTDLPFRMRAYAHGAG
CCCCCCCCCCCEEECCHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCHHHHHHHCCCH
LVVSEMVASGELAKGRAESARRIRRPDIDVHMVQIAGREARWMAEAARIAVDEGADIVDI
HHHHHHHHCCCCCCCHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHCCCCCCEEEE
NMGCPAKKVTGGYSGSALMREPDHALSLIEAVVKAVDVPVTVKMRLGWDDACLNAPELAR
CCCCCHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEEECCCHHHCCCHHHHH
RAEAAGVAMVTVHGRTRCQFYTGQANWKAIAEVKQAVSIPVVANGDGEGPLEAATMLTQS
HHHHCCEEEEEEECCEEEEEEECCCCHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHCC
GADAVMIGRAHYGAPWKAGALAHAADGSAAPGVPAGTAEMADYVAAHYVDMLSHYGTGQG
CCCEEEEECCCCCCCCCCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCC
TRHARKHIGWYLDRHSAPDPALRRSIMTETDPSKVLHLIGGIFSQPGLVQGLAA
HHHHHHHHHHHEECCCCCCHHHHHHHHCCCCHHHHHHHHHHHHCCCCCHHHCCC
>Mature Secondary Structure 
SNKWAFSKDALFLSNSALQILSQPLQVGAVRLRNRVFLAPMSGVTDLPFRMRAYAHGAG
CCCCCCCCCCEEECCHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCHHHHHHHCCCH
LVVSEMVASGELAKGRAESARRIRRPDIDVHMVQIAGREARWMAEAARIAVDEGADIVDI
HHHHHHHHCCCCCCCHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHCCCCCCEEEE
NMGCPAKKVTGGYSGSALMREPDHALSLIEAVVKAVDVPVTVKMRLGWDDACLNAPELAR
CCCCCHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEEECCCHHHCCCHHHHH
RAEAAGVAMVTVHGRTRCQFYTGQANWKAIAEVKQAVSIPVVANGDGEGPLEAATMLTQS
HHHHCCEEEEEEECCEEEEEEECCCCHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHCC
GADAVMIGRAHYGAPWKAGALAHAADGSAAPGVPAGTAEMADYVAAHYVDMLSHYGTGQG
CCCEEEEECCCCCCCCCCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCC
TRHARKHIGWYLDRHSAPDPALRRSIMTETDPSKVLHLIGGIFSQPGLVQGLAA
HHHHHHHHHHHEECCCCCCHHHHHHHHCCCCHHHHHHHHHHHHCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8412703 [H]