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The map label for this gene is ntrC [H]

Identifier: 110633970

GI number: 110633970

Start: 1743569

End: 1745029

Strand: Reverse

Name: ntrC [H]

Synonym: Meso_1618

Alternate gene names: 110633970

Gene position: 1745029-1743569 (Counterclockwise)

Preceding gene: 110633971

Following gene: 110633969

Centisome position: 39.55

GC content: 61.33

Gene sequence:

>1461_bases
ATGAGCGCACAAGGCAACATTCTGGTGGCCGATGACGACGCGGCCATTCGCACGGTCCTCAATCAGGCGCTGTCGCGCGT
GGGGCACCAGGTCCGCGTGACGTCGAATGCCGCGACGCTTTGGCGCTGGGTTGCCGCGGGCGAGGGGGATCTAGTCATTA
CCGATGTGGTGATGCCGGACGAAAATGCCTTCGACATGCTTCCGCGCATCCGAAAAGCGAGGCCGGACCTGCCGGTCATC
GTCATGAGCGCACAGAACACCTTCATGACTGCGATTCGTGCGTCGGAGGCAGGCGCTTACGAATACCTGCCCAAGCCCTT
CGATTTGACGGAGCTTCTGGGCATAGTCGGTCGGGCTCTGGCAGAGCCGCGCAGCAAGGCAGTCGAGACGCGGACGAACG
ATACGCCGGAAGCGATGCCGCTCATCGGCCGCTCGCCGGCCATGCAGGATATCTACCGCATGCTTGCCCGCATGATGCAG
ACGGATCTCACCGTCATGATCACCGGCGAATCGGGGACTGGCAAGGAACTCGTCGCGCGGGCTCTACATGAATTCGGCCG
CCGCCGTAACGGCCCCTTCGTCGCTATCAACATGGCGGCCATACCCCGGGACCTGATCGAGTCGGAACTCTTCGGCCACG
AGAAAGGGGCTTTCACGGGCGCGCAGCAGCGCTCTTCCGGTCGCTTCGAGCAAGCTGAAGGTGGCACGCTTTTCCTCGAT
GAAATCGGCGATATGCCCATGGAGGCCCAAACGCGGCTCCTGCGAGTGCTCCAGCAGGGCGAATACACCACGGTGGGCGG
ACGCACGCCGATCAAGACCGATGTGCGAATCGTTGCTGCCACGAACAAGGATTTGCGCGCGCTTATCAATCAGGGGCTTT
TTCGTGAAGATCTCTTCTACCGCCTGAATGTCGTCCCGCTGCGGCTGCCGCCGTTGCGCGAAAGGTCGGAGGATATACCC
GATCTGGTGCGTCATTTCTTTGCACAAGCGGAACGGGAAGGACTCCAGCCCAAGCGCATCGCCACGGGCGGGGTCGACGC
CATGATGAAATATCCATGGCCCGGCAATGTGCGCGAACTGGAAAACCTAGTGCGCCGGTTGGCCGCGCTATACCCCCAGG
ACGAGATCTCCGAGGAAATCATACTGGGCGAGCTTCATGGTGGGATGCCGGCCGCCAATTCGCCTTTGAATGATGGAGCG
GGGCAGGACCTGCCACTTGGGCAATCCGTGGAACAATATCTGCAGCGCTATTTCCGAGGCTTCGGTTCAGCGCTTCCGCC
GCCGGGCCTCTACCACCGCATTCTGGCCGAGGTGGAGTATCCACTGATCCTTGCGGCTCTCGCTGCCACCCATGGCAATC
AGATCCGGGCGGCCGAACTGCTTGGGGTCAACCGCAACACACTGCGCAAGAAGATCCGCGATCTTAGCGTCGATATCTAT
CGGACGGCCAAAAACCCCTGA

Upstream 100 bases:

>100_bases
CGTCCTCACGCGGAACGACTTTCCGTGTGCTCATGCCGGCGTGGCGTGGCGATCCGCCGTCGTTGGTGGCGGGCCGGCGG
AAGAAAACGGATGCGAAGGC

Downstream 100 bases:

>100_bases
ATTGAGACCACAGCTTTAGGCGCAGCAACAAGCTCGTTGCAAAATCGCCACATTGCGTTGCATATCTGCAACGCAAAGAC
TCTGCAGGTGGGATAGGGAT

Product: nitrogen metabolism transcriptional regulator, NtrC, Fis family

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 486; Mature: 485

Protein sequence:

>486_residues
MSAQGNILVADDDAAIRTVLNQALSRVGHQVRVTSNAATLWRWVAAGEGDLVITDVVMPDENAFDMLPRIRKARPDLPVI
VMSAQNTFMTAIRASEAGAYEYLPKPFDLTELLGIVGRALAEPRSKAVETRTNDTPEAMPLIGRSPAMQDIYRMLARMMQ
TDLTVMITGESGTGKELVARALHEFGRRRNGPFVAINMAAIPRDLIESELFGHEKGAFTGAQQRSSGRFEQAEGGTLFLD
EIGDMPMEAQTRLLRVLQQGEYTTVGGRTPIKTDVRIVAATNKDLRALINQGLFREDLFYRLNVVPLRLPPLRERSEDIP
DLVRHFFAQAEREGLQPKRIATGGVDAMMKYPWPGNVRELENLVRRLAALYPQDEISEEIILGELHGGMPAANSPLNDGA
GQDLPLGQSVEQYLQRYFRGFGSALPPPGLYHRILAEVEYPLILAALAATHGNQIRAAELLGVNRNTLRKKIRDLSVDIY
RTAKNP

Sequences:

>Translated_486_residues
MSAQGNILVADDDAAIRTVLNQALSRVGHQVRVTSNAATLWRWVAAGEGDLVITDVVMPDENAFDMLPRIRKARPDLPVI
VMSAQNTFMTAIRASEAGAYEYLPKPFDLTELLGIVGRALAEPRSKAVETRTNDTPEAMPLIGRSPAMQDIYRMLARMMQ
TDLTVMITGESGTGKELVARALHEFGRRRNGPFVAINMAAIPRDLIESELFGHEKGAFTGAQQRSSGRFEQAEGGTLFLD
EIGDMPMEAQTRLLRVLQQGEYTTVGGRTPIKTDVRIVAATNKDLRALINQGLFREDLFYRLNVVPLRLPPLRERSEDIP
DLVRHFFAQAEREGLQPKRIATGGVDAMMKYPWPGNVRELENLVRRLAALYPQDEISEEIILGELHGGMPAANSPLNDGA
GQDLPLGQSVEQYLQRYFRGFGSALPPPGLYHRILAEVEYPLILAALAATHGNQIRAAELLGVNRNTLRKKIRDLSVDIY
RTAKNP
>Mature_485_residues
SAQGNILVADDDAAIRTVLNQALSRVGHQVRVTSNAATLWRWVAAGEGDLVITDVVMPDENAFDMLPRIRKARPDLPVIV
MSAQNTFMTAIRASEAGAYEYLPKPFDLTELLGIVGRALAEPRSKAVETRTNDTPEAMPLIGRSPAMQDIYRMLARMMQT
DLTVMITGESGTGKELVARALHEFGRRRNGPFVAINMAAIPRDLIESELFGHEKGAFTGAQQRSSGRFEQAEGGTLFLDE
IGDMPMEAQTRLLRVLQQGEYTTVGGRTPIKTDVRIVAATNKDLRALINQGLFREDLFYRLNVVPLRLPPLRERSEDIPD
LVRHFFAQAEREGLQPKRIATGGVDAMMKYPWPGNVRELENLVRRLAALYPQDEISEEIILGELHGGMPAANSPLNDGAG
QDLPLGQSVEQYLQRYFRGFGSALPPPGLYHRILAEVEYPLILAALAATHGNQIRAAELLGVNRNTLRKKIRDLSVDIYR
TAKNP

Specific function: Member of the two-component regulatory system ntrB/ntrC involved in the activation of nitrogen assimilatory genes such as glnA. NtrC is phosphorylated by ntrB and interacts with sigma-54 [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=482, Percent_Identity=43.9834024896266, Blast_Score=392, Evalue=1e-110,
Organism=Escherichia coli, GI1788550, Length=486, Percent_Identity=39.0946502057613, Blast_Score=323, Evalue=2e-89,
Organism=Escherichia coli, GI1790437, Length=468, Percent_Identity=42.0940170940171, Blast_Score=322, Evalue=2e-89,
Organism=Escherichia coli, GI1788905, Length=384, Percent_Identity=39.5833333333333, Blast_Score=272, Evalue=3e-74,
Organism=Escherichia coli, GI1789233, Length=342, Percent_Identity=42.3976608187134, Blast_Score=250, Evalue=1e-67,
Organism=Escherichia coli, GI87082117, Length=231, Percent_Identity=53.2467532467532, Blast_Score=248, Evalue=4e-67,
Organism=Escherichia coli, GI1789087, Length=354, Percent_Identity=41.2429378531073, Blast_Score=241, Evalue=7e-65,
Organism=Escherichia coli, GI87082152, Length=256, Percent_Identity=46.484375, Blast_Score=230, Evalue=2e-61,
Organism=Escherichia coli, GI87081872, Length=243, Percent_Identity=45.679012345679, Blast_Score=214, Evalue=1e-56,
Organism=Escherichia coli, GI1786524, Length=248, Percent_Identity=45.9677419354839, Blast_Score=214, Evalue=1e-56,
Organism=Escherichia coli, GI1787583, Length=227, Percent_Identity=41.4096916299559, Blast_Score=185, Evalue=5e-48,
Organism=Escherichia coli, GI1789828, Length=232, Percent_Identity=35.3448275862069, Blast_Score=138, Evalue=7e-34,
Organism=Escherichia coli, GI87081858, Length=322, Percent_Identity=28.5714285714286, Blast_Score=123, Evalue=3e-29,
Organism=Escherichia coli, GI1789809, Length=114, Percent_Identity=33.3333333333333, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI1786784, Length=232, Percent_Identity=24.1379310344828, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI1790552, Length=137, Percent_Identity=30.6569343065693, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR010114
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 53825; Mature: 53693

Theoretical pI: Translated: 6.66; Mature: 6.66

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAQGNILVADDDAAIRTVLNQALSRVGHQVRVTSNAATLWRWVAAGEGDLVITDVVMPD
CCCCCCEEEECCHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHCCCCCCEEEEEEECCC
ENAFDMLPRIRKARPDLPVIVMSAQNTFMTAIRASEAGAYEYLPKPFDLTELLGIVGRAL
CCHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHCCCCCHHCCCCCCCHHHHHHHHHHHH
AEPRSKAVETRTNDTPEAMPLIGRSPAMQDIYRMLARMMQTDLTVMITGESGTGKELVAR
HCHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCEEEEEECCCCCCHHHHHH
ALHEFGRRRNGPFVAINMAAIPRDLIESELFGHEKGAFTGAQQRSSGRFEQAEGGTLFLD
HHHHHHHCCCCCEEEEEHHHCCHHHHHHHHHCCCCCCCCCCHHHCCCCCCCCCCCEEEHH
EIGDMPMEAQTRLLRVLQQGEYTTVGGRTPIKTDVRIVAATNKDLRALINQGLFREDLFY
HHCCCCHHHHHHHHHHHHCCCEEECCCCCCCCCCEEEEEECCHHHHHHHHCCCHHHHHHH
RLNVVPLRLPPLRERSEDIPDLVRHFFAQAEREGLQPKRIATGGVDAMMKYPWPGNVREL
EEEEEEECCCCCHHHCCCHHHHHHHHHHHHHHCCCCCCCEECCCCHHHEECCCCCCHHHH
ENLVRRLAALYPQDEISEEIILGELHGGMPAANSPLNDGAGQDLPLGQSVEQYLQRYFRG
HHHHHHHHHHCCHHHHHHHHEEHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
FGSALPPPGLYHRILAEVEYPLILAALAATHGNQIRAAELLGVNRNTLRKKIRDLSVDIY
HCCCCCCCHHHHHHHHHCCHHHHHHHHHHHCCCCEEHHHHHCCCHHHHHHHHHHHCCEEE
RTAKNP
ECCCCC
>Mature Secondary Structure 
SAQGNILVADDDAAIRTVLNQALSRVGHQVRVTSNAATLWRWVAAGEGDLVITDVVMPD
CCCCCEEEECCHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHCCCCCCEEEEEEECCC
ENAFDMLPRIRKARPDLPVIVMSAQNTFMTAIRASEAGAYEYLPKPFDLTELLGIVGRAL
CCHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHCCCCCHHCCCCCCCHHHHHHHHHHHH
AEPRSKAVETRTNDTPEAMPLIGRSPAMQDIYRMLARMMQTDLTVMITGESGTGKELVAR
HCHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCEEEEEECCCCCCHHHHHH
ALHEFGRRRNGPFVAINMAAIPRDLIESELFGHEKGAFTGAQQRSSGRFEQAEGGTLFLD
HHHHHHHCCCCCEEEEEHHHCCHHHHHHHHHCCCCCCCCCCHHHCCCCCCCCCCCEEEHH
EIGDMPMEAQTRLLRVLQQGEYTTVGGRTPIKTDVRIVAATNKDLRALINQGLFREDLFY
HHCCCCHHHHHHHHHHHHCCCEEECCCCCCCCCCEEEEEECCHHHHHHHHCCCHHHHHHH
RLNVVPLRLPPLRERSEDIPDLVRHFFAQAEREGLQPKRIATGGVDAMMKYPWPGNVREL
EEEEEEECCCCCHHHCCCHHHHHHHHHHHHHHCCCCCCCEECCCCHHHEECCCCCCHHHH
ENLVRRLAALYPQDEISEEIILGELHGGMPAANSPLNDGAGQDLPLGQSVEQYLQRYFRG
HHHHHHHHHHCCHHHHHHHHEEHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
FGSALPPPGLYHRILAEVEYPLILAALAATHGNQIRAAELLGVNRNTLRKKIRDLSVDIY
HCCCCCCCHHHHHHHHHCCHHHHHHHHHHHCCCCEEHHHHHCCCHHHHHHHHHHHCCEEE
RTAKNP
ECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2881918; 11481430; 8478331 [H]