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The map label for this gene is pyrB

Identifier: 110633712

GI number: 110633712

Start: 1452192

End: 1453151

Strand: Reverse

Name: pyrB

Synonym: Meso_1359

Alternate gene names: 110633712

Gene position: 1453151-1452192 (Counterclockwise)

Preceding gene: 110633714

Following gene: 110633711

Centisome position: 32.93

GC content: 64.27

Gene sequence:

>960_bases
ATGACTGACGCGCCGGGCTTTCCCCTCTTCCCTCACCCCCACCTCCTGGGCATCAAGGGCCTTTCGCCCGCCGATATCGA
GCTCCTGCTCGAGCGGGCCGACGCTGCCGTTGCGATCTCGCGGCGGCCGGAAAAGAAGCTCGCCACCTTGCGCGGGCGCA
CCCAGATCAATCTCTTCTTTGAGGCATCCACCCGGACCCAGTCTTCCTTCGAGCTGGCGGGGAAGCGGCTTGGAGCCGAT
GTCATGAACATGTCGGTCGCAAGCTCCTCGACCAAAAAGGGCGAGACGCTTCTCGATACGGCGATGACGCTCAACGCCAT
GCGGCCTGATATCCTTGTGGTTCGCCATTCCGCGGCGGGCGCGGCGGCGCTGCTTGCGCAGAAGGTAGGCTGCTCGGTGG
TGAATGCTGGCGACGGCGCGCATGAACACCCCACGCAGGCCCTTCTCGACGCGCTCACCATACGCCGCAAGAAAGGCAGC
ATCGCTGGGCTCGTCGTCGCGATCTGCGGTGACGTTCTCCACTCGCGCGTCGCGCGATCAAACATCCTTCTTCTCAACGC
GCTCGGCGCGCGTGTGCGCGTCATTGCCCCTTCCACCCTTCTGCCCTCCGGCATCGGCCAGATGGGGGTCGAGCCCTTCA
CGAAAATGGGCGAAGGGCTTGCCGGGGCCGATGTTGTGATGATGCTGAGGCTCCAGCGCGAGCGCATGGCCGGCACCTTC
GTGCCTTCCGTGCGGGAATATTTCCGCTTCTTCGGCCTCGATGCCGAAAAGCTCAAAGCCGCCAAGGAGGATGCGCTGGT
CATGCATCCCGGACCGATGAACCGCGGAGTCGAGATCGCTTCGGAAGTGGCTGACGGTCCGCAAAGCGTGATCCAGGAAC
AGGTGGAGATGGGCGTTGCCGTACGCATGGCGGTGATGGAAGCCCTGCTCGATCCGCGCCGCAACGGGGACGGAGCATGA

Upstream 100 bases:

>100_bases
ATCTCTTTTTCTTTCCCGCACAAAATGGCGGGAAGAAACTGCGCACAGGGTTTTCGGTGCTTGCGGCCCGGGCGCTGCGG
GCCTATAGCAGCGCTTCAAG

Downstream 100 bases:

>100_bases
GCGTCACCGTCTTCACCAACGCACGCCTGGTCGACCCCTCGCGCGGTCTCGACGAAATCGGCACGCTGATCGTCGATGGC
GAAAACATCGTCGCCTCCGG

Product: aspartate carbamoyltransferase catalytic subunit

Products: NA

Alternate protein names: Aspartate transcarbamylase; ATCase

Number of amino acids: Translated: 319; Mature: 318

Protein sequence:

>319_residues
MTDAPGFPLFPHPHLLGIKGLSPADIELLLERADAAVAISRRPEKKLATLRGRTQINLFFEASTRTQSSFELAGKRLGAD
VMNMSVASSSTKKGETLLDTAMTLNAMRPDILVVRHSAAGAAALLAQKVGCSVVNAGDGAHEHPTQALLDALTIRRKKGS
IAGLVVAICGDVLHSRVARSNILLLNALGARVRVIAPSTLLPSGIGQMGVEPFTKMGEGLAGADVVMMLRLQRERMAGTF
VPSVREYFRFFGLDAEKLKAAKEDALVMHPGPMNRGVEIASEVADGPQSVIQEQVEMGVAVRMAVMEALLDPRRNGDGA

Sequences:

>Translated_319_residues
MTDAPGFPLFPHPHLLGIKGLSPADIELLLERADAAVAISRRPEKKLATLRGRTQINLFFEASTRTQSSFELAGKRLGAD
VMNMSVASSSTKKGETLLDTAMTLNAMRPDILVVRHSAAGAAALLAQKVGCSVVNAGDGAHEHPTQALLDALTIRRKKGS
IAGLVVAICGDVLHSRVARSNILLLNALGARVRVIAPSTLLPSGIGQMGVEPFTKMGEGLAGADVVMMLRLQRERMAGTF
VPSVREYFRFFGLDAEKLKAAKEDALVMHPGPMNRGVEIASEVADGPQSVIQEQVEMGVAVRMAVMEALLDPRRNGDGA
>Mature_318_residues
TDAPGFPLFPHPHLLGIKGLSPADIELLLERADAAVAISRRPEKKLATLRGRTQINLFFEASTRTQSSFELAGKRLGADV
MNMSVASSSTKKGETLLDTAMTLNAMRPDILVVRHSAAGAAALLAQKVGCSVVNAGDGAHEHPTQALLDALTIRRKKGSI
AGLVVAICGDVLHSRVARSNILLLNALGARVRVIAPSTLLPSGIGQMGVEPFTKMGEGLAGADVVMMLRLQRERMAGTFV
PSVREYFRFFGLDAEKLKAAKEDALVMHPGPMNRGVEIASEVADGPQSVIQEQVEMGVAVRMAVMEALLDPRRNGDGA

Specific function: Pyrimidine biosynthesis; second step. [C]

COG id: COG0540

COG function: function code F; Aspartate carbamoyltransferase, catalytic chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family

Homologues:

Organism=Homo sapiens, GI18105007, Length=307, Percent_Identity=35.8306188925081, Blast_Score=172, Evalue=4e-43,
Organism=Escherichia coli, GI2367364, Length=318, Percent_Identity=35.5345911949685, Blast_Score=147, Evalue=1e-36,
Organism=Escherichia coli, GI1790703, Length=197, Percent_Identity=29.9492385786802, Blast_Score=64, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI193204318, Length=324, Percent_Identity=34.8765432098765, Blast_Score=143, Evalue=9e-35,
Organism=Saccharomyces cerevisiae, GI6322331, Length=308, Percent_Identity=35.7142857142857, Blast_Score=155, Evalue=7e-39,
Organism=Drosophila melanogaster, GI24642586, Length=275, Percent_Identity=36.3636363636364, Blast_Score=149, Evalue=2e-36,

Paralogues:

None

Copy number: 2080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 14328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): PYRB_MESSB (Q11IM0)

Other databases:

- EMBL:   CP000390
- RefSeq:   YP_673920.1
- ProteinModelPortal:   Q11IM0
- SMR:   Q11IM0
- STRING:   Q11IM0
- GeneID:   4181554
- GenomeReviews:   CP000390_GR
- KEGG:   mes:Meso_1359
- NMPDR:   fig|266779.1.peg.2233
- eggNOG:   COG0540
- HOGENOM:   HBG579429
- OMA:   MNRGLEI
- ProtClustDB:   PRK00856
- BioCyc:   MSP266779:MESO_1359-MONOMER
- HAMAP:   MF_00001
- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002082
- PRINTS:   PR00100
- PRINTS:   PR00101
- TIGRFAMs:   TIGR00670

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N; SSF53671 Asp/Orn_carbamoyltranf

EC number: =2.1.3.2

Molecular weight: Translated: 33917; Mature: 33786

Theoretical pI: Translated: 9.67; Mature: 9.67

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDAPGFPLFPHPHLLGIKGLSPADIELLLERADAAVAISRRPEKKLATLRGRTQINLFF
CCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHCHHEEECCCCHHHHHHHCCCEEEEEEE
EASTRTQSSFELAGKRLGADVMNMSVASSSTKKGETLLDTAMTLNAMRPDILVVRHSAAG
EECCCCCHHHHHHHHHHCHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCC
AAALLAQKVGCSVVNAGDGAHEHPTQALLDALTIRRKKGSIAGLVVAICGDVLHSRVARS
HHHHHHHHHCCEEEECCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHC
NILLLNALGARVRVIAPSTLLPSGIGQMGVEPFTKMGEGLAGADVVMMLRLQRERMAGTF
CEEEEEECCCEEEEEECCHHCCCCCHHCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHCC
VPSVREYFRFFGLDAEKLKAAKEDALVMHPGPMNRGVEIASEVADGPQSVIQEQVEMGVA
CHHHHHHHHHHCCCHHHHHHHHCCCEEECCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHH
VRMAVMEALLDPRRNGDGA
HHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
TDAPGFPLFPHPHLLGIKGLSPADIELLLERADAAVAISRRPEKKLATLRGRTQINLFF
CCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHCHHEEECCCCHHHHHHHCCCEEEEEEE
EASTRTQSSFELAGKRLGADVMNMSVASSSTKKGETLLDTAMTLNAMRPDILVVRHSAAG
EECCCCCHHHHHHHHHHCHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCC
AAALLAQKVGCSVVNAGDGAHEHPTQALLDALTIRRKKGSIAGLVVAICGDVLHSRVARS
HHHHHHHHHCCEEEECCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHC
NILLLNALGARVRVIAPSTLLPSGIGQMGVEPFTKMGEGLAGADVVMMLRLQRERMAGTF
CEEEEEECCCEEEEEECCHHCCCCCHHCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHCC
VPSVREYFRFFGLDAEKLKAAKEDALVMHPGPMNRGVEIASEVADGPQSVIQEQVEMGVA
CHHHHHHHHHHCCCHHHHHHHHCCCEEECCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHH
VRMAVMEALLDPRRNGDGA
HHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA