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The map label for this gene is metZ [H]

Identifier: 110632460

GI number: 110632460

Start: 128168

End: 129358

Strand: Direct

Name: metZ [H]

Synonym: Meso_0098

Alternate gene names: 110632460

Gene position: 128168-129358 (Clockwise)

Preceding gene: 110632458

Following gene: 110632461

Centisome position: 2.9

GC content: 61.63

Gene sequence:

>1191_bases
ATGACAAAAAGATTCAACGACGACTGGAAGCCTCAAACCCGCCTTGTTCATGGCGGCACACTGCGCTCCTCCTTTGGCGA
AACCTCCGAAGCCCTCTATCTTACCCAGGGTTTTGTCTATGAGACCGCCGAGGCGGCCGAAGCGCGCTTCAAGGGTGAGG
ATCCGGGTTTCATCTACTCGCGCTATGCCAATCCCACGGTGGACATGTTCGAGAGCCGCATGTGCGCGCTCGAAGAGGCC
GAGGATGCACGCGCTACCGCCTCCGGGATGGCCGCCGTGGCAGCGGCCCTCCTGTGTTCGGTCAAGGCCGGCGACCATGT
GGTGGCAGCGCGCGCGCTTTTCGGCTCCTGCCGCTGGATCGTGGAAACGCTGATGCCGCGCTACGGCATCGAGACGACGC
TCGTCGACGGTACGAGGATGGAGGCGTGGGAGGAGGCGGTGCGCAAGAACACAAAGCTGTTCTTCCTGGAAAGCCCGACC
AACCCGACGCTCGAAATTTGCGATATTGCCAGTATCGCCAAGCTTGCCGACAGCATCGGCGCGCGGCTGGTGGTGGATAA
CGTCTTTGCCACGCCGCTCCTGCAGAAGCCGTTGGAACTGGGCGCGCATGTGGTGGTCTATTCCGCAACGAAGCATATCG
ATGGCCAGGGGCGCTGTCTCGGCGGCGTCGTGCTTTCCGACAAGGAATGGATCGACGAGAACCTGCACGACTACTTCCGG
CATACCGGCCCCAGCCTTTCGCCCTTCAACGCATGGACGCTGCTCAAGGGTCTGGAGACGCTACCCATGCGCGTGCGCCA
GCAGATGGAAAGCGCGGCGCACGTTGCGGATTACCTGGCCGATCATCCGAAGGTCGAGCGCGTGATCTATCCCGGCCGGC
CCGATCATCCGCAGGCCGATCTCGTAAAGCGTCAGATGAAGGGCGGCTCCACGCTCGTCTGCCTTGACATCAAGGGCGGC
AAGCAGGGTGCTTTCGCATTTTCAAACGCCCTTGAGATCATCCGCATTTCCAACAATCTCGGCGACGCCAAGAGCCTGAT
CACGCACCCGGCCACCACAACGCACAAGAACCTCTCGGAGGAAGCGCGGCTCGAAGCCGGGATCCAGCAGGGAACACTGC
GCCTGTCGCTCGGCCTGGAAGATACGGAAGACCTCTTGAAGGATATCGAGCAGGCGCTGGCGACCGTGTAA

Upstream 100 bases:

>100_bases
TGGCCGGTCGGCTTGCAGCCACGTTAAAGAAGTCGCTAAAAAGGCCGTGCTCGAAGCTCGAACCGGCTTTCCTGCGGCCG
GTTTTTTGTTGAGCGGAGCG

Downstream 100 bases:

>100_bases
ATGCGCAACCGCGTCGGTGCACCGTCCGTGAACAGATCGCCCGAAAGGCATCGGGCGCGGATGTGGTTCTCCCTGGCGCC
GGCGGTCGTTTCAGTGCTGC

Product: O-succinylhomoserine sulfhydrylase

Products: Cystathionine; succinate; o-succinyl-L-homoserine; H2O [C]

Alternate protein names: OSH sulfhydrylase [H]

Number of amino acids: Translated: 396; Mature: 395

Protein sequence:

>396_residues
MTKRFNDDWKPQTRLVHGGTLRSSFGETSEALYLTQGFVYETAEAAEARFKGEDPGFIYSRYANPTVDMFESRMCALEEA
EDARATASGMAAVAAALLCSVKAGDHVVAARALFGSCRWIVETLMPRYGIETTLVDGTRMEAWEEAVRKNTKLFFLESPT
NPTLEICDIASIAKLADSIGARLVVDNVFATPLLQKPLELGAHVVVYSATKHIDGQGRCLGGVVLSDKEWIDENLHDYFR
HTGPSLSPFNAWTLLKGLETLPMRVRQQMESAAHVADYLADHPKVERVIYPGRPDHPQADLVKRQMKGGSTLVCLDIKGG
KQGAFAFSNALEIIRISNNLGDAKSLITHPATTTHKNLSEEARLEAGIQQGTLRLSLGLEDTEDLLKDIEQALATV

Sequences:

>Translated_396_residues
MTKRFNDDWKPQTRLVHGGTLRSSFGETSEALYLTQGFVYETAEAAEARFKGEDPGFIYSRYANPTVDMFESRMCALEEA
EDARATASGMAAVAAALLCSVKAGDHVVAARALFGSCRWIVETLMPRYGIETTLVDGTRMEAWEEAVRKNTKLFFLESPT
NPTLEICDIASIAKLADSIGARLVVDNVFATPLLQKPLELGAHVVVYSATKHIDGQGRCLGGVVLSDKEWIDENLHDYFR
HTGPSLSPFNAWTLLKGLETLPMRVRQQMESAAHVADYLADHPKVERVIYPGRPDHPQADLVKRQMKGGSTLVCLDIKGG
KQGAFAFSNALEIIRISNNLGDAKSLITHPATTTHKNLSEEARLEAGIQQGTLRLSLGLEDTEDLLKDIEQALATV
>Mature_395_residues
TKRFNDDWKPQTRLVHGGTLRSSFGETSEALYLTQGFVYETAEAAEARFKGEDPGFIYSRYANPTVDMFESRMCALEEAE
DARATASGMAAVAAALLCSVKAGDHVVAARALFGSCRWIVETLMPRYGIETTLVDGTRMEAWEEAVRKNTKLFFLESPTN
PTLEICDIASIAKLADSIGARLVVDNVFATPLLQKPLELGAHVVVYSATKHIDGQGRCLGGVVLSDKEWIDENLHDYFRH
TGPSLSPFNAWTLLKGLETLPMRVRQQMESAAHVADYLADHPKVERVIYPGRPDHPQADLVKRQMKGGSTLVCLDIKGGK
QGAFAFSNALEIIRISNNLGDAKSLITHPATTTHKNLSEEARLEAGIQQGTLRLSLGLEDTEDLLKDIEQALATV

Specific function: Converts O-succinylhomoserine into homocysteine [H]

COG id: COG0626

COG function: function code E; Cystathionine beta-lyases/cystathionine gamma-synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=343, Percent_Identity=37.3177842565598, Blast_Score=205, Evalue=7e-53,
Organism=Homo sapiens, GI299473759, Length=343, Percent_Identity=35.8600583090379, Blast_Score=176, Evalue=3e-44,
Organism=Homo sapiens, GI25453487, Length=342, Percent_Identity=31.8713450292398, Blast_Score=151, Evalue=1e-36,
Organism=Escherichia coli, GI1790375, Length=335, Percent_Identity=34.3283582089552, Blast_Score=207, Evalue=1e-54,
Organism=Escherichia coli, GI1789383, Length=398, Percent_Identity=27.6381909547739, Blast_Score=139, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI17538055, Length=345, Percent_Identity=37.1014492753623, Blast_Score=233, Evalue=2e-61,
Organism=Caenorhabditis elegans, GI17560056, Length=351, Percent_Identity=35.3276353276353, Blast_Score=224, Evalue=9e-59,
Organism=Caenorhabditis elegans, GI71980708, Length=371, Percent_Identity=33.1536388140162, Blast_Score=198, Evalue=5e-51,
Organism=Saccharomyces cerevisiae, GI6319307, Length=400, Percent_Identity=34.5, Blast_Score=219, Evalue=5e-58,
Organism=Saccharomyces cerevisiae, GI6323333, Length=410, Percent_Identity=30.4878048780488, Blast_Score=174, Evalue=3e-44,
Organism=Saccharomyces cerevisiae, GI6321254, Length=406, Percent_Identity=26.3546798029557, Blast_Score=128, Evalue=1e-30,
Organism=Saccharomyces cerevisiae, GI6321904, Length=395, Percent_Identity=28.3544303797468, Blast_Score=126, Evalue=6e-30,
Organism=Saccharomyces cerevisiae, GI6321170, Length=270, Percent_Identity=28.5185185185185, Blast_Score=124, Evalue=2e-29,
Organism=Drosophila melanogaster, GI19922554, Length=344, Percent_Identity=36.3372093023256, Blast_Score=233, Evalue=2e-61,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006234
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: 2.5.1.48 [C]

Molecular weight: Translated: 43478; Mature: 43347

Theoretical pI: Translated: 5.63; Mature: 5.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKRFNDDWKPQTRLVHGGTLRSSFGETSEALYLTQGFVYETAEAAEARFKGEDPGFIYS
CCCCCCCCCCCCEEEEECCCHHHHCCCCCCEEEEECCCHHHHHHHHHHHCCCCCCCEEEE
RYANPTVDMFESRMCALEEAEDARATASGMAAVAAALLCSVKAGDHVVAARALFGSCRWI
ECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
VETLMPRYGIETTLVDGTRMEAWEEAVRKNTKLFFLESPTNPTLEICDIASIAKLADSIG
HHHHHHCCCCCEEEECCCHHHHHHHHHHCCCEEEEEECCCCCCEEHHHHHHHHHHHHHHC
ARLVVDNVFATPLLQKPLELGAHVVVYSATKHIDGQGRCLGGVVLSDKEWIDENLHDYFR
CEEEEHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCEEEEEEECCHHHHHHHHHHHHH
HTGPSLSPFNAWTLLKGLETLPMRVRQQMESAAHVADYLADHPKVERVIYPGRPDHPQAD
HCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCHHH
LVKRQMKGGSTLVCLDIKGGKQGAFAFSNALEIIRISNNLGDAKSLITHPATTTHKNLSE
HHHHHHCCCCEEEEEEECCCCCCCHHHHCCEEEEEECCCCCHHHHHHCCCCCCCCCCCHH
EARLEAGIQQGTLRLSLGLEDTEDLLKDIEQALATV
HHHHHHCHHCCEEEEEECCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TKRFNDDWKPQTRLVHGGTLRSSFGETSEALYLTQGFVYETAEAAEARFKGEDPGFIYS
CCCCCCCCCCCEEEEECCCHHHHCCCCCCEEEEECCCHHHHHHHHHHHCCCCCCCEEEE
RYANPTVDMFESRMCALEEAEDARATASGMAAVAAALLCSVKAGDHVVAARALFGSCRWI
ECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
VETLMPRYGIETTLVDGTRMEAWEEAVRKNTKLFFLESPTNPTLEICDIASIAKLADSIG
HHHHHHCCCCCEEEECCCHHHHHHHHHHCCCEEEEEECCCCCCEEHHHHHHHHHHHHHHC
ARLVVDNVFATPLLQKPLELGAHVVVYSATKHIDGQGRCLGGVVLSDKEWIDENLHDYFR
CEEEEHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCEEEEEEECCHHHHHHHHHHHHH
HTGPSLSPFNAWTLLKGLETLPMRVRQQMESAAHVADYLADHPKVERVIYPGRPDHPQAD
HCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCHHH
LVKRQMKGGSTLVCLDIKGGKQGAFAFSNALEIIRISNNLGDAKSLITHPATTTHKNLSE
HHHHHHCCCCEEEEEEECCCCCCCHHHHCCEEEEEECCCCCHHHHHHCCCCCCCCCCCHH
EARLEAGIQQGTLRLSLGLEDTEDLLKDIEQALATV
HHHHHHCHHCCEEEEEECCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: pyridoxal 5'-phosphate [C]

Metal ions: NA

Kcat value (1/min): 460 [C]

Specific activity: 10

Km value (mM): 0.33 {O-succinyl-L-homoserine}} [C]

Substrates: O-succinyl-L-homoserine; @CYST01.txt*L-cysteine!; 2-oxobutanoate; succinate; NH3 [C]

Specific reaction: O-succinyl-L-homoserine + @CYST01.txt*L-cysteine! = cystathionine + succinate 2-oxobutanoate + succinate + NH3 = o-succinyl-L-homoserine + H2O [C]

General reaction: Elimination; Gamma-replacement [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7704274; 10984043 [H]