Click here to switch to the map view.

The map label for this gene is argF [H]

Identifier: 110632370

GI number: 110632370

Start: 7199

End: 8107

Strand: Reverse

Name: argF [H]

Synonym: Meso_0008

Alternate gene names: 110632370

Gene position: 8107-7199 (Counterclockwise)

Preceding gene: 110632371

Following gene: 110632369

Centisome position: 0.18

GC content: 61.17

Gene sequence:

>909_bases
TTGGCACGCAATTTCATCGATCTTTCCGCCGTTTCTGCCGCCGATCTGCGGTCCATTCTGGACGATGCCAAGGAGCGCAA
GGCGCTGCTGAAATCAGGGAAGGCCGGCAAGCCCTTCGAAGGCAAAGTGCTTGCGATGATCTTCGACAAGCCTTCGACCC
GCACGCGCGTTTCGTTTGATGTGGCGATGCGCCAGCTCGGCGGCGATACGATCATGCTTACCGGCATGGAAATGCAGCTC
GGTCGCAGCGAGACGATCGCCGACACGGCCAAGGTGCTTTCGCGCTACGTCGATGCCATCATGATCCGCACCACGGCCCA
TGAGAGGCTTCTGGAGCTTGCCGAAAACGCCACCGTACCCGTCATCAACGGGCTTACTGACGACACGCATCCGTGCCAGA
TCATGGCTGATATCATGACATTCGAGGAGCATCGCGGCGATGTGAAGGACCGGCTCTTCGCGTGGTCGGGCGATGGCAAC
AACGTGCTGCATTCCCTCCTAGAGGCGTCGGCACGCTTCGGTTTCAGGTTAAACATCGCCACACCGGTTGGCAGCGAGCC
CTCCGCTCACTATGTGGAGTGGGCAAAGACGAATGGCGGACGCGTGACCCTCACCAGCGACCCGGAAGCGGCGGTGCGCG
GGGCCGATTGCGTGGTGACCGACACCTGGATCTCAATGGGCCAGGAGCACCGCGCGCGAGGACACAATGTGTTCCAACCC
TATCAAGTGAACGAGAGGCTGATGAGCTTCGCTAAGAAAGAGGCACTTTTCATGCACTGCCTGCCCGCCCATCGCGGCGA
GGAGGTGACCGACGGCGTTATCGACGGCCCCCACTCGGTGGTGTTCGACGAGGCGGAGAACCGCTTGCACGCCCAGAAAG
CCGTTCTCGCCTGGTGCCTCGGCCAGTGA

Upstream 100 bases:

>100_bases
CTGATGAAGATATTCAGGAGGGGCTGGAGCGTATCCATCGCGCCATGGAAAGCTTGGTATCTGCGTCCTCAATTGCCACA
GCGACAAAGGTGTGACTGCC

Downstream 100 bases:

>100_bases
ACGACGATACGGCGAAATTGGGCGACTTCGGCTTCGCCGGAGACGATCACGTCGTGCCTTTCGAGGTGGAGATGCTCGAC
GTGCGCGGGCGCGCCGTGCA

Product: ornithine carbamoyltransferase

Products: NA

Alternate protein names: OTCase [H]

Number of amino acids: Translated: 302; Mature: 301

Protein sequence:

>302_residues
MARNFIDLSAVSAADLRSILDDAKERKALLKSGKAGKPFEGKVLAMIFDKPSTRTRVSFDVAMRQLGGDTIMLTGMEMQL
GRSETIADTAKVLSRYVDAIMIRTTAHERLLELAENATVPVINGLTDDTHPCQIMADIMTFEEHRGDVKDRLFAWSGDGN
NVLHSLLEASARFGFRLNIATPVGSEPSAHYVEWAKTNGGRVTLTSDPEAAVRGADCVVTDTWISMGQEHRARGHNVFQP
YQVNERLMSFAKKEALFMHCLPAHRGEEVTDGVIDGPHSVVFDEAENRLHAQKAVLAWCLGQ

Sequences:

>Translated_302_residues
MARNFIDLSAVSAADLRSILDDAKERKALLKSGKAGKPFEGKVLAMIFDKPSTRTRVSFDVAMRQLGGDTIMLTGMEMQL
GRSETIADTAKVLSRYVDAIMIRTTAHERLLELAENATVPVINGLTDDTHPCQIMADIMTFEEHRGDVKDRLFAWSGDGN
NVLHSLLEASARFGFRLNIATPVGSEPSAHYVEWAKTNGGRVTLTSDPEAAVRGADCVVTDTWISMGQEHRARGHNVFQP
YQVNERLMSFAKKEALFMHCLPAHRGEEVTDGVIDGPHSVVFDEAENRLHAQKAVLAWCLGQ
>Mature_301_residues
ARNFIDLSAVSAADLRSILDDAKERKALLKSGKAGKPFEGKVLAMIFDKPSTRTRVSFDVAMRQLGGDTIMLTGMEMQLG
RSETIADTAKVLSRYVDAIMIRTTAHERLLELAENATVPVINGLTDDTHPCQIMADIMTFEEHRGDVKDRLFAWSGDGNN
VLHSLLEASARFGFRLNIATPVGSEPSAHYVEWAKTNGGRVTLTSDPEAAVRGADCVVTDTWISMGQEHRARGHNVFQPY
QVNERLMSFAKKEALFMHCLPAHRGEEVTDGVIDGPHSVVFDEAENRLHAQKAVLAWCLGQ

Specific function: Arginine biosynthesis; sixth step. [C]

COG id: COG0078

COG function: function code E; Ornithine carbamoyltransferase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family [H]

Homologues:

Organism=Homo sapiens, GI38788445, Length=305, Percent_Identity=40.655737704918, Blast_Score=230, Evalue=1e-60,
Organism=Homo sapiens, GI18105007, Length=271, Percent_Identity=26.9372693726937, Blast_Score=87, Evalue=2e-17,
Organism=Escherichia coli, GI1790703, Length=328, Percent_Identity=39.9390243902439, Blast_Score=210, Evalue=7e-56,
Organism=Escherichia coli, GI1786469, Length=328, Percent_Identity=39.0243902439024, Blast_Score=204, Evalue=6e-54,
Organism=Escherichia coli, GI2367364, Length=311, Percent_Identity=29.5819935691318, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI48994908, Length=328, Percent_Identity=27.7439024390244, Blast_Score=84, Evalue=8e-18,
Organism=Caenorhabditis elegans, GI193204318, Length=326, Percent_Identity=26.0736196319018, Blast_Score=75, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6322373, Length=309, Percent_Identity=36.5695792880259, Blast_Score=195, Evalue=7e-51,
Organism=Saccharomyces cerevisiae, GI6322331, Length=307, Percent_Identity=25.4071661237785, Blast_Score=88, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24642586, Length=295, Percent_Identity=27.1186440677966, Blast_Score=93, Evalue=2e-19,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2066 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002292 [H]

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N [H]

EC number: =2.1.3.3 [H]

Molecular weight: Translated: 33292; Mature: 33161

Theoretical pI: Translated: 6.29; Mature: 6.29

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARNFIDLSAVSAADLRSILDDAKERKALLKSGKAGKPFEGKVLAMIFDKPSTRTRVSFD
CCCCEECHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCEEEHHH
VAMRQLGGDTIMLTGMEMQLGRSETIADTAKVLSRYVDAIMIRTTAHERLLELAENATVP
HHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCE
VINGLTDDTHPCQIMADIMTFEEHRGDVKDRLFAWSGDGNNVLHSLLEASARFGFRLNIA
EEECCCCCCCHHHHHHHHHHHHHHCCCHHHHEEEECCCCHHHHHHHHHHHHCCCEEEEEE
TPVGSEPSAHYVEWAKTNGGRVTLTSDPEAAVRGADCVVTDTWISMGQEHRARGHNVFQP
CCCCCCCCCCEEEEEECCCCEEEECCCCHHHHCCCCEEEEHHHHHCCHHHHHCCCCCCCC
YQVNERLMSFAKKEALFMHCLPAHRGEEVTDGVIDGPHSVVFDEAENRLHAQKAVLAWCL
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHC
GQ
CC
>Mature Secondary Structure 
ARNFIDLSAVSAADLRSILDDAKERKALLKSGKAGKPFEGKVLAMIFDKPSTRTRVSFD
CCCEECHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCEEEHHH
VAMRQLGGDTIMLTGMEMQLGRSETIADTAKVLSRYVDAIMIRTTAHERLLELAENATVP
HHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCE
VINGLTDDTHPCQIMADIMTFEEHRGDVKDRLFAWSGDGNNVLHSLLEASARFGFRLNIA
EEECCCCCCCHHHHHHHHHHHHHHCCCHHHHEEEECCCCHHHHHHHHHHHHCCCEEEEEE
TPVGSEPSAHYVEWAKTNGGRVTLTSDPEAAVRGADCVVTDTWISMGQEHRARGHNVFQP
CCCCCCCCCCEEEEEECCCCEEEECCCCHHHHCCCCEEEEHHHHHCCHHHHHCCCCCCCC
YQVNERLMSFAKKEALFMHCLPAHRGEEVTDGVIDGPHSVVFDEAENRLHAQKAVLAWCL
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHC
GQ
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11214968 [H]