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The map label for this gene is tig

Identifier: 109947465

GI number: 109947465

Start: 802694

End: 804049

Strand: Reverse

Name: tig

Synonym: Hac_0917

Alternate gene names: 109947465

Gene position: 804049-802694 (Counterclockwise)

Preceding gene: 109947466

Following gene: 109947464

Centisome position: 51.74

GC content: 35.99

Gene sequence:

>1356_bases
ATGAATCTTGAAGTAAAAAAGATTGACACCGCTAACTCCCATTTGAGCGCTAAGCCTTCCATTGAAGATTTAAAAAAGCG
TTATGATAAAATCGCTCAAAAAATCGCCCAAAAAGTTAAGATTGACGGCTTTAGAAGAGGTAAAGTCCCCCTTAGTTTAG
TGAAAACCCGCTATCAAGCTCAAATTGAGCAAGACGCCCAAGAAGAAATGTTTCAAGAGATTTTAAAAAACGCTCTTAAA
GAATTAGGGATTGAATCTAAAGACTTGATCGGTAGTCCCAATCTCACTAAATTTGAAAAAAAAGACACGCATTTTGAAAT
AGAAGCGGACATCGGCTTAAAACCCACGATCGTTTTAGACAAGATCAAAGAGTGTGTGCCTAGCGTGGAAGTGGAAGCGG
TGGATGAAGAAAAGGTTAATGAGCGTTTGAAACAGCTCGCTAAAGACTATGCGAAGTTTATAGACACTGATGCTCAAAGA
AAAGCTCAAAACGATGATAAATTAACGATTGATTTTGAAGGCTTTATAGACAATGTGCCTTTTGAAGGGGGCAAGACTCA
AAATTTTAGTTTGATTTTAGGCAGTAAGCAAATGCTAGAAGATTTTGAAAAAGCTCTTTTAGGCATGCAAGCGGGCGAAG
AAAAAGAATTTTCTTTGACTTTCCCTAGCGATTATCATGCAAAGCATTTAGCTGGCAAAGAAGCTCTCTTTAAAGTGAAA
TTACACCAAATTCAAGCACGTGAAGTGTTAGAAATCAATGATGAACTCGCTAAAATCGTGTTAGCTAATGAAGAAAATGC
GGCGTTAGAGCTTTTAAAAGAAAGGGTTAAGGGGCAATTATTTTTAGAAAATAAGGTCAGACTCTATAATGAAGAGTTAA
AAGAAAAATTGATTGAAAATTTAGATGAAAAAATCCTTTTTGATTTGCCTAAAACGATTATAGAGCAAGAAATGGATTTG
TTATTCAGGAACGCTCTTTATTCCATGCAAGCAGAGGAAGTCAAATCCTTACAAGAAAATCAAGAAAAAGCTAAAGAAAA
GCGTGAAAGTTTTAGGAATGATGCGACAAAAAGCGTGAAAATCACTTTCATTATTGACGCTTTAGCTAAAGAAGAAAAAA
TTGGCGTGCATGATAATGAAGTCTTTCAAACCTTGTATTATGAAGCGCTGATGACAGGGCAAAACCCAGAAAATCTCATT
GAGCAATACCGCCGAAATAACATGTTAGCAGCAGTGAAAATGGCGATGATTGAAGATCGAGTGCTTACTTATTTCTTGGA
TAAAAACCTATCTAAAGAACAGCAAGAAATTTTGGAAAAAATGAGGCCTAACGCTCAAAAAACTCAAGTGAGTTGA

Upstream 100 bases:

>100_bases
AATCCTTTGAAAACTAGAATTAAAAGCGCTTTTTATCAACCGAAATTCAAACAAATTAAGCTATAATGCCTATTTAATTT
TTTAACCATTAAGGAAAAAA

Downstream 100 bases:

>100_bases
ATAATAAAAAGGAGAAATGGTGGGATACATTCCTTATGTGATAGAAAATACTGATCGAGGGGAGCGCAGTTATGATATTT
ACTCGCGCCTTTTAAAGGAT

Product: trigger factor

Products: peptidylproline (w =0) [C]

Alternate protein names: TF

Number of amino acids: Translated: 451; Mature: 451

Protein sequence:

>451_residues
MNLEVKKIDTANSHLSAKPSIEDLKKRYDKIAQKIAQKVKIDGFRRGKVPLSLVKTRYQAQIEQDAQEEMFQEILKNALK
ELGIESKDLIGSPNLTKFEKKDTHFEIEADIGLKPTIVLDKIKECVPSVEVEAVDEEKVNERLKQLAKDYAKFIDTDAQR
KAQNDDKLTIDFEGFIDNVPFEGGKTQNFSLILGSKQMLEDFEKALLGMQAGEEKEFSLTFPSDYHAKHLAGKEALFKVK
LHQIQAREVLEINDELAKIVLANEENAALELLKERVKGQLFLENKVRLYNEELKEKLIENLDEKILFDLPKTIIEQEMDL
LFRNALYSMQAEEVKSLQENQEKAKEKRESFRNDATKSVKITFIIDALAKEEKIGVHDNEVFQTLYYEALMTGQNPENLI
EQYRRNNMLAAVKMAMIEDRVLTYFLDKNLSKEQQEILEKMRPNAQKTQVS

Sequences:

>Translated_451_residues
MNLEVKKIDTANSHLSAKPSIEDLKKRYDKIAQKIAQKVKIDGFRRGKVPLSLVKTRYQAQIEQDAQEEMFQEILKNALK
ELGIESKDLIGSPNLTKFEKKDTHFEIEADIGLKPTIVLDKIKECVPSVEVEAVDEEKVNERLKQLAKDYAKFIDTDAQR
KAQNDDKLTIDFEGFIDNVPFEGGKTQNFSLILGSKQMLEDFEKALLGMQAGEEKEFSLTFPSDYHAKHLAGKEALFKVK
LHQIQAREVLEINDELAKIVLANEENAALELLKERVKGQLFLENKVRLYNEELKEKLIENLDEKILFDLPKTIIEQEMDL
LFRNALYSMQAEEVKSLQENQEKAKEKRESFRNDATKSVKITFIIDALAKEEKIGVHDNEVFQTLYYEALMTGQNPENLI
EQYRRNNMLAAVKMAMIEDRVLTYFLDKNLSKEQQEILEKMRPNAQKTQVS
>Mature_451_residues
MNLEVKKIDTANSHLSAKPSIEDLKKRYDKIAQKIAQKVKIDGFRRGKVPLSLVKTRYQAQIEQDAQEEMFQEILKNALK
ELGIESKDLIGSPNLTKFEKKDTHFEIEADIGLKPTIVLDKIKECVPSVEVEAVDEEKVNERLKQLAKDYAKFIDTDAQR
KAQNDDKLTIDFEGFIDNVPFEGGKTQNFSLILGSKQMLEDFEKALLGMQAGEEKEFSLTFPSDYHAKHLAGKEALFKVK
LHQIQAREVLEINDELAKIVLANEENAALELLKERVKGQLFLENKVRLYNEELKEKLIENLDEKILFDLPKTIIEQEMDL
LFRNALYSMQAEEVKSLQENQEKAKEKRESFRNDATKSVKITFIIDALAKEEKIGVHDNEVFQTLYYEALMTGQNPENLI
EQYRRNNMLAAVKMAMIEDRVLTYFLDKNLSKEQQEILEKMRPNAQKTQVS

Specific function: Involved in protein export. Acts as a chaperone by maintaining the newly synthesized protein in an open conformation

COG id: COG0544

COG function: function code O; FKBP-type peptidyl-prolyl cis-trans isomerase (trigger factor)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PPIase FKBP-type domain

Homologues:

Organism=Escherichia coli, GI1786640, Length=382, Percent_Identity=30.6282722513089, Blast_Score=152, Evalue=5e-38,

Paralogues:

None

Copy number: 1070 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 500 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 340 Molecules/Cell In: Growth-Phase, Mini

Swissprot (AC and ID): TIG_HELAH (Q17XD2)

Other databases:

- EMBL:   AM260522
- RefSeq:   YP_664693.1
- ProteinModelPortal:   Q17XD2
- STRING:   Q17XD2
- GeneID:   4177479
- GenomeReviews:   AM260522_GR
- KEGG:   hac:Hac_0917
- NMPDR:   fig|382638.8.peg.891
- eggNOG:   COG0544
- HOGENOM:   HBG635105
- OMA:   RFIPGFE
- PhylomeDB:   Q17XD2
- ProtClustDB:   PRK01490
- BioCyc:   HACI382638:HAC_0917-MONOMER
- HAMAP:   MF_00303
- InterPro:   IPR001179
- InterPro:   IPR005215
- InterPro:   IPR008880
- InterPro:   IPR008881
- Gene3D:   G3DSA:1.10.3120.10
- Gene3D:   G3DSA:3.30.70.1050
- PIRSF:   PIRSF003095
- TIGRFAMs:   TIGR00115

Pfam domain/function: PF00254 FKBP_C; PF05698 Trigger_C; PF05697 Trigger_N; SSF109998 Trigger_fac_C_bac; SSF102735 Trigger_fac_ribosome-bd_bac

EC number: NA

Molecular weight: Translated: 52063; Mature: 52063

Theoretical pI: Translated: 5.07; Mature: 5.07

Prosite motif: PS50059 FKBP_PPIASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLEVKKIDTANSHLSAKPSIEDLKKRYDKIAQKIAQKVKIDGFRRGKVPLSLVKTRYQA
CCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHH
QIEQDAQEEMFQEILKNALKELGIESKDLIGSPNLTKFEKKDTHFEIEADIGLKPTIVLD
HHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCCCCCCCCCCCCCCEEEEECCCCCCHHHHH
KIKECVPSVEVEAVDEEKVNERLKQLAKDYAKFIDTDAQRKAQNDDKLTIDFEGFIDNVP
HHHHHCCCCEEEECCHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCEEEEEECCCCCCCC
FEGGKTQNFSLILGSKQMLEDFEKALLGMQAGEEKEFSLTFPSDYHAKHLAGKEALFKVK
CCCCCCCCEEEEECCHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCHHHHCCHHHHHHHH
LHQIQAREVLEINDELAKIVLANEENAALELLKERVKGQLFLENKVRLYNEELKEKLIEN
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCEEEEHHHHHHHHHHHHHHHHHH
LDEKILFDLPKTIIEQEMDLLFRNALYSMQAEEVKSLQENQEKAKEKRESFRNDATKSVK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEE
ITFIIDALAKEEKIGVHDNEVFQTLYYEALMTGQNPENLIEQYRRNNMLAAVKMAMIEDR
EEEEEHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHHHH
VLTYFLDKNLSKEQQEILEKMRPNAQKTQVS
HHHHHHHCCCCHHHHHHHHHHCCCCHHCCCC
>Mature Secondary Structure
MNLEVKKIDTANSHLSAKPSIEDLKKRYDKIAQKIAQKVKIDGFRRGKVPLSLVKTRYQA
CCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHH
QIEQDAQEEMFQEILKNALKELGIESKDLIGSPNLTKFEKKDTHFEIEADIGLKPTIVLD
HHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCCCCCCCCCCCCCCEEEEECCCCCCHHHHH
KIKECVPSVEVEAVDEEKVNERLKQLAKDYAKFIDTDAQRKAQNDDKLTIDFEGFIDNVP
HHHHHCCCCEEEECCHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCEEEEEECCCCCCCC
FEGGKTQNFSLILGSKQMLEDFEKALLGMQAGEEKEFSLTFPSDYHAKHLAGKEALFKVK
CCCCCCCCEEEEECCHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCHHHHCCHHHHHHHH
LHQIQAREVLEINDELAKIVLANEENAALELLKERVKGQLFLENKVRLYNEELKEKLIEN
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCEEEEHHHHHHHHHHHHHHHHHH
LDEKILFDLPKTIIEQEMDLLFRNALYSMQAEEVKSLQENQEKAKEKRESFRNDATKSVK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEE
ITFIIDALAKEEKIGVHDNEVFQTLYYEALMTGQNPENLIEQYRRNNMLAAVKMAMIEDR
EEEEEHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHHHH
VLTYFLDKNLSKEQQEILEKMRPNAQKTQVS
HHHHHHHCCCCHHHHHHHHHHCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: peptidylproline (w =180) [C]

Specific reaction: peptidylproline (w =180) = peptidylproline (w =0) [C]

General reaction: Isomerases; cis-trans-Isomerases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA