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The map label for this gene is hyaD [H]

Identifier: 109947315

GI number: 109947315

Start: 656189

End: 656725

Strand: Direct

Name: hyaD [H]

Synonym: Hac_0747

Alternate gene names: 109947315

Gene position: 656189-656725 (Clockwise)

Preceding gene: 109947314

Following gene: 109947316

Centisome position: 42.23

GC content: 40.97

Gene sequence:

>537_bases
ATGAGTCAAAAAATCTTAATTCTAGGCATTGGCAATATCCTTTTTGGCGATGAAGGGATTGGAGTGCATTTAGCCCATTA
TCTCAAAAGGAATTTTTCTTTTTTTCCTAGCGTGGATATTGTAGATGGGGGGACTATGGCTCAGCAACTCATTCCTTTAA
TCACTTCGTATGAAAAGGTTTTGATTTTAGATTGCGTGAGCGCTAAAGGCGTTGAAATAGGATCGGTGTATGCTTTTGAA
TTTAATGACGCCCCTAAAGAAATCACATGGGCTGGGAGTGCGCATGAAGTGGAAATGCTGCACACTTTAAGGCTTACGGA
GTTTTTAGGGGATTTGCCTGAGACTTTTATTGTGGGGCTTGTGCCTTTTGTGATAGGGAGCGAGACCACTTTCAAGCTTT
CACAAGAGATTTTAAACGCTTTAGAAACAGCCCTACAAGCCATAGAAACCCAACTAAAAGCATGGGGAGTGGGTATGCAA
CGCACAAATAATATTGCATTAGATTGTATCGCTGAACTTTCCTACAAGGGTTTTTGA

Upstream 100 bases:

>100_bases
TATTTATCCCTGTGCATGTTTATATGGTGTTTTTCCATTCTATTAGGTATGATAGTTCAGGGGCGGATTCTATGATTAAT
GGCTATGGTTATACCAAAGA

Downstream 100 bases:

>100_bases
ATTGGTGTTTGTTTTTCTTTTTAAATGCGTTGATGAAAAAACAAACTTGATTTTTACGCCCCTTTTAGAGCAAATGGCAC
TCAATTTACAAGCGGGTTTT

Product: hydrogenase expression/formation protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 178; Mature: 177

Protein sequence:

>178_residues
MSQKILILGIGNILFGDEGIGVHLAHYLKRNFSFFPSVDIVDGGTMAQQLIPLITSYEKVLILDCVSAKGVEIGSVYAFE
FNDAPKEITWAGSAHEVEMLHTLRLTEFLGDLPETFIVGLVPFVIGSETTFKLSQEILNALETALQAIETQLKAWGVGMQ
RTNNIALDCIAELSYKGF

Sequences:

>Translated_178_residues
MSQKILILGIGNILFGDEGIGVHLAHYLKRNFSFFPSVDIVDGGTMAQQLIPLITSYEKVLILDCVSAKGVEIGSVYAFE
FNDAPKEITWAGSAHEVEMLHTLRLTEFLGDLPETFIVGLVPFVIGSETTFKLSQEILNALETALQAIETQLKAWGVGMQ
RTNNIALDCIAELSYKGF
>Mature_177_residues
SQKILILGIGNILFGDEGIGVHLAHYLKRNFSFFPSVDIVDGGTMAQQLIPLITSYEKVLILDCVSAKGVEIGSVYAFEF
NDAPKEITWAGSAHEVEMLHTLRLTEFLGDLPETFIVGLVPFVIGSETTFKLSQEILNALETALQAIETQLKAWGVGMQR
TNNIALDCIAELSYKGF

Specific function: Protease Involved In The C-Terminal Processing Of Hyab, The Large Subunit Of Hydrogenase 1 (Potential). [C]

COG id: COG0680

COG function: function code C; Ni,Fe-hydrogenase maturation factor

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase A31 family [H]

Homologues:

Organism=Escherichia coli, GI1787209, Length=180, Percent_Identity=31.6666666666667, Blast_Score=72, Evalue=2e-14,
Organism=Escherichia coli, GI1789367, Length=155, Percent_Identity=28.3870967741935, Blast_Score=70, Evalue=6e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004419
- InterPro:   IPR000671 [H]

Pfam domain/function: PF01750 HycI [H]

EC number: 3.4.24.- [C]

Molecular weight: Translated: 19539; Mature: 19408

Theoretical pI: Translated: 4.44; Mature: 4.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQKILILGIGNILFGDEGIGVHLAHYLKRNFSFFPSVDIVDGGTMAQQLIPLITSYEKV
CCCCEEEEEECCEEECCCCCHHHHHHHHHHCCCCCCCCEEECCCHHHHHHHHHHHCCCCE
LILDCVSAKGVEIGSVYAFEFNDAPKEITWAGSAHEVEMLHTLRLTEFLGDLPETFIVGL
EEEEECCCCCCEECEEEEEEECCCCCEEEECCCCHHHHHHHHHHHHHHHHCCHHHHHHHH
VPFVIGSETTFKLSQEILNALETALQAIETQLKAWGVGMQRTNNIALDCIAELSYKGF
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCC
>Mature Secondary Structure 
SQKILILGIGNILFGDEGIGVHLAHYLKRNFSFFPSVDIVDGGTMAQQLIPLITSYEKV
CCCEEEEEECCEEECCCCCHHHHHHHHHHCCCCCCCCEEECCCHHHHHHHHHHHCCCCE
LILDCVSAKGVEIGSVYAFEFNDAPKEITWAGSAHEVEMLHTLRLTEFLGDLPETFIVGL
EEEEECCCCCCEECEEEEEEECCCCCEEEECCCCHHHHHHHHHHHHHHHHCCHHHHHHHH
VPFVIGSETTFKLSQEILNALETALQAIETQLKAWGVGMQRTNNIALDCIAELSYKGF
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Ni [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1587288; 9639603; 14500908 [H]