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The map label for this gene is hyaC [H]

Identifier: 109947314

GI number: 109947314

Start: 655524

End: 656192

Strand: Direct

Name: hyaC [H]

Synonym: Hac_0746

Alternate gene names: 109947314

Gene position: 655524-656192 (Clockwise)

Preceding gene: 109947313

Following gene: 109947315

Centisome position: 42.18

GC content: 37.82

Gene sequence:

>669_bases
ATGGATAAAAAGGTCGTTTTACACAAAGAGTATTCAGGTTTTGTGCGCTTTTTCCATTGGGTTAGGGCTTTGAGTATTTT
TATTTTAATTATTACAGGGCTTTACATCGCCTACCCTTTTTTACAACCTGGATCCAGCTTTTATAAAGAAATGTATTTTT
TGCAAGCTTATATCCGCTCTTTTCATGTCATGTTTGGGTTTTTACTCATTAGCGCGTTATTCTTTAGAACTTATCTTTTT
TTCACTAAAGAAAGCGTTAATGAGCGCAGGAGTTTTAGCCAACTTTTGAGTTTAAAAGCGTGGGTAGAACAAATGAAAGC
GTATTTTCTTATTAGTGGCAAACCCCACACTAAAGGAGCGTATAACCCTATCCAACTCGTGGCTTATTTTACCCTAACTA
TTTTGGTGGTGTTGATGAGTTTGAGTGGGGTGGTGCTTTATTATAATGTCTATCACGCTGGGCTTGGGGCATTTTTAGAG
AGTGCTTTTAAGTGGTTTGAGGTGCTTTGTGGGGGGTTAGCGAATGTGCGTTTTATCCACCATTTAGCGACTTGGGGGTT
TGTTTTATTTATCCCTGTGCATGTTTATATGGTGTTTTTCCATTCTATTAGGTATGATAGTTCAGGGGCGGATTCTATGA
TTAATGGCTATGGTTATACCAAAGAATGA

Upstream 100 bases:

>100_bases
ATGCATCGCATGCTCAGTGCATGTAATGGATTTTAAAGGGCAGTCTTTAAACGAGTTTAAAGTAGAGCCTAATTTTGCTA
AATTCTAAAAAGGGATACGC

Downstream 100 bases:

>100_bases
GTCAAAAAATCTTAATTCTAGGCATTGGCAATATCCTTTTTGGCGATGAAGGGATTGGAGTGCATTTAGCCCATTATCTC
AAAAGGAATTTTTCTTTTTT

Product: Ni/Fe hydrogenase cytochrome b subunit

Products: oxidized ferredoxin; H2 [C]

Alternate protein names: NA

Number of amino acids: Translated: 222; Mature: 222

Protein sequence:

>222_residues
MDKKVVLHKEYSGFVRFFHWVRALSIFILIITGLYIAYPFLQPGSSFYKEMYFLQAYIRSFHVMFGFLLISALFFRTYLF
FTKESVNERRSFSQLLSLKAWVEQMKAYFLISGKPHTKGAYNPIQLVAYFTLTILVVLMSLSGVVLYYNVYHAGLGAFLE
SAFKWFEVLCGGLANVRFIHHLATWGFVLFIPVHVYMVFFHSIRYDSSGADSMINGYGYTKE

Sequences:

>Translated_222_residues
MDKKVVLHKEYSGFVRFFHWVRALSIFILIITGLYIAYPFLQPGSSFYKEMYFLQAYIRSFHVMFGFLLISALFFRTYLF
FTKESVNERRSFSQLLSLKAWVEQMKAYFLISGKPHTKGAYNPIQLVAYFTLTILVVLMSLSGVVLYYNVYHAGLGAFLE
SAFKWFEVLCGGLANVRFIHHLATWGFVLFIPVHVYMVFFHSIRYDSSGADSMINGYGYTKE
>Mature_222_residues
MDKKVVLHKEYSGFVRFFHWVRALSIFILIITGLYIAYPFLQPGSSFYKEMYFLQAYIRSFHVMFGFLLISALFFRTYLF
FTKESVNERRSFSQLLSLKAWVEQMKAYFLISGKPHTKGAYNPIQLVAYFTLTILVVLMSLSGVVLYYNVYHAGLGAFLE
SAFKWFEVLCGGLANVRFIHHLATWGFVLFIPVHVYMVFFHSIRYDSSGADSMINGYGYTKE

Specific function: B-type cytochrome that interacts with quinones and anchor the hydrogenase to the membrane [H]

COG id: COG1969

COG function: function code C; Ni,Fe-hydrogenase I cytochrome b subunit

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hupC/hyaC/hydC family [H]

Homologues:

Organism=Escherichia coli, GI1787208, Length=220, Percent_Identity=26.3636363636364, Blast_Score=76, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005797
- InterPro:   IPR016174
- InterPro:   IPR000516 [H]

Pfam domain/function: PF00033 Cytochrom_B_N [H]

EC number: NA

Molecular weight: Translated: 25844; Mature: 25844

Theoretical pI: Translated: 9.75; Mature: 9.75

Prosite motif: PS00882 NI_HGENASE_CYTB_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKKVVLHKEYSGFVRFFHWVRALSIFILIITGLYIAYPFLQPGSSFYKEMYFLQAYIRS
CCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
FHVMFGFLLISALFFRTYLFFTKESVNERRSFSQLLSLKAWVEQMKAYFLISGKPHTKGA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCC
YNPIQLVAYFTLTILVVLMSLSGVVLYYNVYHAGLGAFLESAFKWFEVLCGGLANVRFIH
CCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HLATWGFVLFIPVHVYMVFFHSIRYDSSGADSMINGYGYTKE
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCCC
>Mature Secondary Structure
MDKKVVLHKEYSGFVRFFHWVRALSIFILIITGLYIAYPFLQPGSSFYKEMYFLQAYIRS
CCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
FHVMFGFLLISALFFRTYLFFTKESVNERRSFSQLLSLKAWVEQMKAYFLISGKPHTKGA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCC
YNPIQLVAYFTLTILVVLMSLSGVVLYYNVYHAGLGAFLESAFKWFEVLCGGLANVRFIH
CCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HLATWGFVLFIPVHVYMVFFHSIRYDSSGADSMINGYGYTKE
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Reduced ferredoxin; 2 H+ [C]

Specific reaction: Reduced ferredoxin + 2 H+ = 2 oxidized ferredoxin + H2 [C]

General reaction: Oxidoreductases [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 1587288; 8319698; 14500908 [H]