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The map label for this gene is prfB

Identifier: 109946962

GI number: 109946962

Start: 299102

End: 300193

Strand: Reverse

Name: prfB

Synonym: Hac_0352

Alternate gene names: 109946962

Gene position: 300193-299102 (Counterclockwise)

Preceding gene: 109946963

Following gene: 109946961

Centisome position: 19.32

GC content: 40.84

Gene sequence:

>1092_bases
GTGGATAACTACACTTATAGCGAATTGTTAAAAAGCTTGCAAAACAAATGCGATAATATCGCCTTAATCATCAAGCCTGA
AAAGGTCAAACAGAAATTAGAACGCATTGAAAAAGAGCAAGAAGACCCTAATTTTTGGCAAGATGTCTTAAAAGCCAGAG
ACACCAATAAAGAAAAAGTGCGCTTAAACCGCTTGCTAGAAACCTATCAAAAAACCAAAAACGCTTTAGATGAAAGCGTA
GAATTGTTTGAGATCGCCCAAAACGATAACGATGAAGTTACTTTGTCTTTACTTTATGAAGAGGCTCCTATTTTAGAACA
TGGTGTGCAAAAGGTAGAAATTGAAATCATGTTAAGCGGCGAACATGACGCTTCAAACGCCATTATCACCATTCAGCCTG
GAGCGGGGGGGACTGAAAGCCAGGATTGGGCGAGTATTTTGTATCGCATGTATTTGAGGTGGGCAGAAAGAAGGGGTTTT
AAAAGTGAGATTTTAGATTATCAAGATGGCGAAGAAGCAGGCATTAAAGGGGTAGCCTTTATTATTAAGGGCGAAAACGC
TTATGGCTATTTGAAAAATGAAAGCGGTGTGCATAGGCTTGTTAGGATCTCACCCTTTGATGCAAACGCCAAACGGCACA
CGAGTTTTGCGAGCGTGCAAATTAGCCCTGAATTGGATGATGATATTGATATAGAAATTGATGAAAAAGATGTCCGCTAT
GATTATTATAGGGCTAGTGGGGCAGGTGGTCAGCATGTCAATAAAACAGAAAGCGCGGTTAGGATCACGCATTTTCCCAC
CGGTATTGTGGTGCAATGCCAAAACGACAGAAGCCAACATAAAAACAAAGCGAGCGCCTTAAAAATGCTTAAATCCAAGC
TTTATGAATTGGAATTAGAAAAACAACAAAGCACCGCCAAAAATGAAGAAAAAAGCGAGATCGGTTGGGGGCATCAAATA
AGAAGCTATGTCCTAGCCCCCTACCAGCAAGTCAAAGACGCTCGCTCCAATATCGCTTATAGCAATGTGGAAGCGATTTT
AGATGGCGATATTGACGCCATTTTAGAGGGTGTTTTGATCGCTAAAGCTTAA

Upstream 100 bases:

>100_bases
AATCAGTTTGGCGTTTAGTACGATTAGTAAAATCTTTTAAAATAGCCTCTAACAATGCTATAATGACCACTTTAAAACCC
CACTTATAAAGGTTCTATTT

Downstream 100 bases:

>100_bases
GTGCGCTAAAATAACAGCAATGCATTATGGTTAAAAACGCTTTTAAACGCACAAGGAATAACGATGACACAAGAAGAATT
AGACGCTTTAATGAGTGGGG

Product: peptide chain release factor 2

Products: NA

Alternate protein names: RF-2

Number of amino acids: Translated: 363; Mature: 363

Protein sequence:

>363_residues
MDNYTYSELLKSLQNKCDNIALIIKPEKVKQKLERIEKEQEDPNFWQDVLKARDTNKEKVRLNRLLETYQKTKNALDESV
ELFEIAQNDNDEVTLSLLYEEAPILEHGVQKVEIEIMLSGEHDASNAIITIQPGAGGTESQDWASILYRMYLRWAERRGF
KSEILDYQDGEEAGIKGVAFIIKGENAYGYLKNESGVHRLVRISPFDANAKRHTSFASVQISPELDDDIDIEIDEKDVRY
DYYRASGAGGQHVNKTESAVRITHFPTGIVVQCQNDRSQHKNKASALKMLKSKLYELELEKQQSTAKNEEKSEIGWGHQI
RSYVLAPYQQVKDARSNIAYSNVEAILDGDIDAILEGVLIAKA

Sequences:

>Translated_363_residues
MDNYTYSELLKSLQNKCDNIALIIKPEKVKQKLERIEKEQEDPNFWQDVLKARDTNKEKVRLNRLLETYQKTKNALDESV
ELFEIAQNDNDEVTLSLLYEEAPILEHGVQKVEIEIMLSGEHDASNAIITIQPGAGGTESQDWASILYRMYLRWAERRGF
KSEILDYQDGEEAGIKGVAFIIKGENAYGYLKNESGVHRLVRISPFDANAKRHTSFASVQISPELDDDIDIEIDEKDVRY
DYYRASGAGGQHVNKTESAVRITHFPTGIVVQCQNDRSQHKNKASALKMLKSKLYELELEKQQSTAKNEEKSEIGWGHQI
RSYVLAPYQQVKDARSNIAYSNVEAILDGDIDAILEGVLIAKA
>Mature_363_residues
MDNYTYSELLKSLQNKCDNIALIIKPEKVKQKLERIEKEQEDPNFWQDVLKARDTNKEKVRLNRLLETYQKTKNALDESV
ELFEIAQNDNDEVTLSLLYEEAPILEHGVQKVEIEIMLSGEHDASNAIITIQPGAGGTESQDWASILYRMYLRWAERRGF
KSEILDYQDGEEAGIKGVAFIIKGENAYGYLKNESGVHRLVRISPFDANAKRHTSFASVQISPELDDDIDIEIDEKDVRY
DYYRASGAGGQHVNKTESAVRITHFPTGIVVQCQNDRSQHKNKASALKMLKSKLYELELEKQQSTAKNEEKSEIGWGHQI
RSYVLAPYQQVKDARSNIAYSNVEAILDGDIDAILEGVLIAKA

Specific function: Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA

COG id: COG1186

COG function: function code J; Protein chain release factor B

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family

Homologues:

Organism=Homo sapiens, GI166795303, Length=277, Percent_Identity=36.4620938628159, Blast_Score=171, Evalue=1e-42,
Organism=Homo sapiens, GI34577120, Length=325, Percent_Identity=32, Blast_Score=152, Evalue=6e-37,
Organism=Homo sapiens, GI166795305, Length=191, Percent_Identity=36.1256544502618, Blast_Score=117, Evalue=2e-26,
Organism=Escherichia coli, GI2367172, Length=354, Percent_Identity=49.4350282485876, Blast_Score=353, Evalue=9e-99,
Organism=Escherichia coli, GI1787462, Length=240, Percent_Identity=40.4166666666667, Blast_Score=195, Evalue=4e-51,
Organism=Caenorhabditis elegans, GI17542784, Length=249, Percent_Identity=30.1204819277108, Blast_Score=105, Evalue=3e-23,
Organism=Saccharomyces cerevisiae, GI6321295, Length=327, Percent_Identity=31.1926605504587, Blast_Score=142, Evalue=1e-34,
Organism=Drosophila melanogaster, GI19921226, Length=211, Percent_Identity=36.9668246445498, Blast_Score=149, Evalue=2e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RF2_HELAH (Q17YT5)

Other databases:

- EMBL:   AM260522
- RefSeq:   YP_664190.1
- ProteinModelPortal:   Q17YT5
- SMR:   Q17YT5
- STRING:   Q17YT5
- GeneID:   4176560
- GenomeReviews:   AM260522_GR
- KEGG:   hac:Hac_0352
- NMPDR:   fig|382638.8.peg.341
- eggNOG:   COG1186
- HOGENOM:   HBG629764
- OMA:   TEMLLRM
- PhylomeDB:   Q17YT5
- ProtClustDB:   PRK00578
- BioCyc:   HACI382638:HAC_0352-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00094
- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR020853
- InterPro:   IPR004374
- PANTHER:   PTHR11075:SF6
- SMART:   SM00937
- TIGRFAMs:   TIGR00020

Pfam domain/function: PF03462 PCRF; PF00472 RF-1

EC number: NA

Molecular weight: Translated: 41286; Mature: 41286

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDNYTYSELLKSLQNKCDNIALIIKPEKVKQKLERIEKEQEDPNFWQDVLKARDTNKEKV
CCCCCHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCHHHH
RLNRLLETYQKTKNALDESVELFEIAQNDNDEVTLSLLYEEAPILEHGVQKVEIEIMLSG
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCHHHCCCEEEEEEEEEEC
EHDASNAIITIQPGAGGTESQDWASILYRMYLRWAERRGFKSEILDYQDGEEAGIKGVAF
CCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCCEEEE
IIKGENAYGYLKNESGVHRLVRISPFDANAKRHTSFASVQISPELDDDIDIEIDEKDVRY
EEECCCCEEEEECCCCCEEEEEECCCCCCCCCCCCEEEEEECCCCCCCCEEEECCCCCEE
DYYRASGAGGQHVNKTESAVRITHFPTGIVVQCQNDRSQHKNKASALKMLKSKLYELELE
EEEECCCCCCCCCCCCCCEEEEEECCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHH
KQQSTAKNEEKSEIGWGHQIRSYVLAPYQQVKDARSNIAYSNVEAILDGDIDAILEGVLI
HHHHHHCCCHHHHCCCHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHHH
AKA
CCC
>Mature Secondary Structure
MDNYTYSELLKSLQNKCDNIALIIKPEKVKQKLERIEKEQEDPNFWQDVLKARDTNKEKV
CCCCCHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCHHHH
RLNRLLETYQKTKNALDESVELFEIAQNDNDEVTLSLLYEEAPILEHGVQKVEIEIMLSG
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCHHHCCCEEEEEEEEEEC
EHDASNAIITIQPGAGGTESQDWASILYRMYLRWAERRGFKSEILDYQDGEEAGIKGVAF
CCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCCEEEE
IIKGENAYGYLKNESGVHRLVRISPFDANAKRHTSFASVQISPELDDDIDIEIDEKDVRY
EEECCCCEEEEECCCCCEEEEEECCCCCCCCCCCCEEEEEECCCCCCCCEEEECCCCCEE
DYYRASGAGGQHVNKTESAVRITHFPTGIVVQCQNDRSQHKNKASALKMLKSKLYELELE
EEEECCCCCCCCCCCCCCEEEEEECCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHH
KQQSTAKNEEKSEIGWGHQIRSYVLAPYQQVKDARSNIAYSNVEAILDGDIDAILEGVLI
HHHHHHCCCHHHHCCCHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHHH
AKA
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA