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The map label for this gene is mboIIR [H]

Identifier: 109946811

GI number: 109946811

Start: 157172

End: 158341

Strand: Direct

Name: mboIIR [H]

Synonym: Hac_0188

Alternate gene names: 109946811

Gene position: 157172-158341 (Clockwise)

Preceding gene: 109946810

Following gene: 109946812

Centisome position: 10.11

GC content: 30.77

Gene sequence:

>1170_bases
TTGCAATTATCCCAACACAATCGTTACGATCAAAATAAAATTAAAATCATTATTCAAGCTATTTTTAATGAAGTGGGAGA
GGATTTTTTACAAATTCGCACCACTGACATGAGCAAACGCCCCAGCAATATAACAGGCGAAGAGATTTATGCAAAAGTGG
TGGATAATATCTGCAAGTCTGAAATATCTCAAGATAATTTAGGAAAAAAGAATCAAGTAGCCCAAGATAGTTTGAGGAAA
AATCTTTTCGTAGATATGCATAGAATGGGGTTGATTGAACGCTACAATAAAAATAAAGAGCTTATAGACCCCTATATTCA
AAGCAATATCAAATATATTCGTTTAACTCCCTTAGCCATAGAATTTTTAAACGCACAAGATTTGTTAAGAAAAAATTTTT
GTTACACGCAAGCTTTAGAAAATCTTTTAAAAGGTTTTGGAGCAGAATGCAGAGAGGTAATGATAGGGCTTGACAATCAT
CACTTAGATATTGAAGAAATGATGTTTTTTGTTACATTTTTAAATATTGAAAATTTTACTAGAAGTGAAATTATAGAATA
TGTTAGAGAATACAGGAGTTTAAGCCGCATCCAAAAAGAAAAATTAAAAGAGTCAGTGCAAAATTATTGCAACCCTAACC
ATTTTAGTGGGAACAAGTTAAATAAAAGAGATTATCATAATTGGAAAAATCAAGCCCAACAAATTTTTAGCTTGCTAGAA
CAAAGCGTATTTTTTGAAACCAATAAAGAGAGGCTTATTTTGAAAACGATCAGTGAAGAAAACAAACAAAACGATAAAAA
GCTCAAACGCTCCATTAAAGAAAAAGCCCTTTATTTTGAAAAACATGGCGTAAAAAAAGAAAAGGGCTTTGAGTTGCACC
ATATTGTGCCTTTGTGTTTGGCTCGCTCTTCTATAGAAGAGTTTGATCTTTTAGATAAATGGGAAAATTTAATCTATATT
GACGCTTTTAACCATGCAAAAATATCTCAAACACAAAATAAACATATTTGTTTGTATTTTAAAAATTGCGATGTGATCTT
ATCTAAAGGCTTCAAAGAAGAACAAGAAAGCCTTTATTTGACTTACATTGAAAATGTATCCTATAAACTTGATTTGCAAA
ATACCATGTTGGAATACAATAAGGATCTATTGCATTCTAAAAACGGCTGA

Upstream 100 bases:

>100_bases
AAAAATAGAAGAAATTTTGCAAGAAATCACCTAGCTTGACCCTAGTAAGGAGTGTTTAAGATTTTTAGTTAATCGTATAA
AAAGCCCTGATTATAGGGGA

Downstream 100 bases:

>100_bases
CAAAATAAAGCGGTGTCTTTTTTAAGAGAGCATTTTTCATAATCGCTTTTAGCATAAAGGCTATCGTATAAGGGCGTGTC
ATTGAAATAAATCCCCCCAT

Product: typeIIS restriction enzyme

Products: NA

Alternate protein names: R.MboII; Endonuclease MboII; Type II restriction enzyme MboII; Type IIS restriction enzyme MboII [H]

Number of amino acids: Translated: 389; Mature: 389

Protein sequence:

>389_residues
MQLSQHNRYDQNKIKIIIQAIFNEVGEDFLQIRTTDMSKRPSNITGEEIYAKVVDNICKSEISQDNLGKKNQVAQDSLRK
NLFVDMHRMGLIERYNKNKELIDPYIQSNIKYIRLTPLAIEFLNAQDLLRKNFCYTQALENLLKGFGAECREVMIGLDNH
HLDIEEMMFFVTFLNIENFTRSEIIEYVREYRSLSRIQKEKLKESVQNYCNPNHFSGNKLNKRDYHNWKNQAQQIFSLLE
QSVFFETNKERLILKTISEENKQNDKKLKRSIKEKALYFEKHGVKKEKGFELHHIVPLCLARSSIEEFDLLDKWENLIYI
DAFNHAKISQTQNKHICLYFKNCDVILSKGFKEEQESLYLTYIENVSYKLDLQNTMLEYNKDLLHSKNG

Sequences:

>Translated_389_residues
MQLSQHNRYDQNKIKIIIQAIFNEVGEDFLQIRTTDMSKRPSNITGEEIYAKVVDNICKSEISQDNLGKKNQVAQDSLRK
NLFVDMHRMGLIERYNKNKELIDPYIQSNIKYIRLTPLAIEFLNAQDLLRKNFCYTQALENLLKGFGAECREVMIGLDNH
HLDIEEMMFFVTFLNIENFTRSEIIEYVREYRSLSRIQKEKLKESVQNYCNPNHFSGNKLNKRDYHNWKNQAQQIFSLLE
QSVFFETNKERLILKTISEENKQNDKKLKRSIKEKALYFEKHGVKKEKGFELHHIVPLCLARSSIEEFDLLDKWENLIYI
DAFNHAKISQTQNKHICLYFKNCDVILSKGFKEEQESLYLTYIENVSYKLDLQNTMLEYNKDLLHSKNG
>Mature_389_residues
MQLSQHNRYDQNKIKIIIQAIFNEVGEDFLQIRTTDMSKRPSNITGEEIYAKVVDNICKSEISQDNLGKKNQVAQDSLRK
NLFVDMHRMGLIERYNKNKELIDPYIQSNIKYIRLTPLAIEFLNAQDLLRKNFCYTQALENLLKGFGAECREVMIGLDNH
HLDIEEMMFFVTFLNIENFTRSEIIEYVREYRSLSRIQKEKLKESVQNYCNPNHFSGNKLNKRDYHNWKNQAQQIFSLLE
QSVFFETNKERLILKTISEENKQNDKKLKRSIKEKALYFEKHGVKKEKGFELHHIVPLCLARSSIEEFDLLDKWENLIYI
DAFNHAKISQTQNKHICLYFKNCDVILSKGFKEEQESLYLTYIENVSYKLDLQNTMLEYNKDLLHSKNG

Specific function: Recognizes the double-stranded sequences GAAGA and TCTTC and cleaves respectively 13 bases after G-1 and 7 bases before T- 1, leaving a single 3' protruding nucleotide [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: =3.1.21.4 [H]

Molecular weight: Translated: 46182; Mature: 46182

Theoretical pI: Translated: 8.06; Mature: 8.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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EEECCCEEEEEHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure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EEECCCEEEEEHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2020540 [H]