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The map label for this gene is mod fragment 2 [H]

Identifier: 109946810

GI number: 109946810

Start: 156528

End: 157043

Strand: Direct

Name: mod fragment 2 [H]

Synonym: Hac_0187

Alternate gene names: 109946810

Gene position: 156528-157043 (Clockwise)

Preceding gene: 109946809

Following gene: 109946811

Centisome position: 10.07

GC content: 37.02

Gene sequence:

>516_bases
GTGCATTTTTTAAATTTTATCACTTGGGTCAAAAAAGATGGGTTTGCCAACGCTAAAAAGCGTTATAACCATGCACAAGA
AAGCATTTTATTTTATAGCATGCATAAGAAAAATTACACTTTTAATGCTGATGAAATTCGCATCGCTTATGAATCCACTG
AACGCATCAAACACGCTCAAAGCAAAGGGATTTTAAAGAATAACAAACGCTGGTTCCCTAACCCTAATGGCAAATTATGC
CTTGATGTGTGGGAGATCGCTTCACAAAGGCATGTTGAAAAAGAGAATGGTAAGATCCTTAAGCCAAAACACCCTAGCAT
CAAACCTAAAGCGCTCATTGAACGCATGATAAAAGCCAGCTCTCACAAAAACGATTTGATTTTAGATTTATTTAGTGGCA
GTGGCATGACTAGCTTGGTGGCTAAAAGTTTAGGGCGTAACTTTATAGGGTGTGAAAGCCATGCTGAGTATGTGCATGAA
AGTTTGGAAATATTAGATATGATAAGTATCACTTAA

Upstream 100 bases:

>100_bases
TTGATAAAATGCTACCCAAAATCAAAGACACAGGGAGTTTTTATATCTTTAATACCCCCTTTAACTGCGCTTTATTTTTA
GCATATTTGTGTCAAAAAAA

Downstream 100 bases:

>100_bases
ATAAGTAAAAAAGGGTATTTGACATGCCAAAAATAGAAGAAATTTTGCAAGAAATCACCTAGCTTGACCCTAGTAAGGAG
TGTTTAAGATTTTTAGTTAA

Product: type IIS restriction enzyme M1 protein fragment 2

Products: NA

Alternate protein names: M.MboII; Adenine-specific methyltransferase MboII; DNA MTase MboIIA [H]

Number of amino acids: Translated: 171; Mature: 171

Protein sequence:

>171_residues
MHFLNFITWVKKDGFANAKKRYNHAQESILFYSMHKKNYTFNADEIRIAYESTERIKHAQSKGILKNNKRWFPNPNGKLC
LDVWEIASQRHVEKENGKILKPKHPSIKPKALIERMIKASSHKNDLILDLFSGSGMTSLVAKSLGRNFIGCESHAEYVHE
SLEILDMISIT

Sequences:

>Translated_171_residues
MHFLNFITWVKKDGFANAKKRYNHAQESILFYSMHKKNYTFNADEIRIAYESTERIKHAQSKGILKNNKRWFPNPNGKLC
LDVWEIASQRHVEKENGKILKPKHPSIKPKALIERMIKASSHKNDLILDLFSGSGMTSLVAKSLGRNFIGCESHAEYVHE
SLEILDMISIT
>Mature_171_residues
MHFLNFITWVKKDGFANAKKRYNHAQESILFYSMHKKNYTFNADEIRIAYESTERIKHAQSKGILKNNKRWFPNPNGKLC
LDVWEIASQRHVEKENGKILKPKHPSIKPKALIERMIKASSHKNDLILDLFSGSGMTSLVAKSLGRNFIGCESHAEYVHE
SLEILDMISIT

Specific function: This methylase recognizes the double-stranded sequence GAAGA, causes specific methylation on A-5, and protects the DNA from cleavage by the MboII endonuclease. It is not known if the cytosine of the complementary sequence TCTTC is also methylated by this

COG id: COG0863

COG function: function code L; DNA modification methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI87082238, Length=167, Percent_Identity=35.9281437125749, Blast_Score=89, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002941
- InterPro:   IPR002052
- InterPro:   IPR001091 [H]

Pfam domain/function: PF01555 N6_N4_Mtase [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 19743; Mature: 19743

Theoretical pI: Translated: 10.12; Mature: 10.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHFLNFITWVKKDGFANAKKRYNHAQESILFYSMHKKNYTFNADEIRIAYESTERIKHAQ
CCHHHHHHHHHCCCCCHHHHHHHHHHHHHEEEEEECCCCEECCCCEEEEEHHHHHHHHHH
SKGILKNNKRWFPNPNGKLCLDVWEIASQRHVEKENGKILKPKHPSIKPKALIERMIKAS
HCCCCCCCCCCCCCCCCCEEHHHHHHHHHHCCHHCCCCEECCCCCCCCHHHHHHHHHHHC
SHKNDLILDLFSGSGMTSLVAKSLGRNFIGCESHAEYVHESLEILDMISIT
CCCCCEEEEEECCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MHFLNFITWVKKDGFANAKKRYNHAQESILFYSMHKKNYTFNADEIRIAYESTERIKHAQ
CCHHHHHHHHHCCCCCHHHHHHHHHHHHHEEEEEECCCCEECCCCEEEEEHHHHHHHHHH
SKGILKNNKRWFPNPNGKLCLDVWEIASQRHVEKENGKILKPKHPSIKPKALIERMIKAS
HCCCCCCCCCCCCCCCCCEEHHHHHHHHHHCCHHCCCCEECCCCCCCCHHHHHHHHHHHC
SHKNDLILDLFSGSGMTSLVAKSLGRNFIGCESHAEYVHESLEILDMISIT
CCCCCEEEEEECCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2020540 [H]