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The map label for this gene is gmd [H]

Identifier: 109946689

GI number: 109946689

Start: 53481

End: 54626

Strand: Direct

Name: gmd [H]

Synonym: Hac_0056

Alternate gene names: 109946689

Gene position: 53481-54626 (Clockwise)

Preceding gene: 109946688

Following gene: 109946690

Centisome position: 3.44

GC content: 40.23

Gene sequence:

>1146_bases
ATGAAAGAAAAAATCGCTTTAATCACTGGGGTTACCGGTCAAGATGGGAGCTATCTGACTGAATACTTGCTGAATTTAGG
CTATGAAGTGCATGGGCTAAAAAGGCGCTCTTCTAGTATCAACACTTCTAGGATCGATCATCTGTATGAAGATTTGCACA
GCGAACACAAAAGGCGTTTTTTCTTACACTATGGGGATATGACAGACAGCTCTAATCTTATCCATTTGATCGCCACCACA
AAGCCTACAGAGATTTATAATTTAGCCGCACAAAGCCATGTAAAAGTCTCTTTTGAAACCCCAGAATACACCGCTAACGC
TGATGGTATTGGCACGCTAAGGATTTTAGAGTCCATGCGGATTTTAGGGCTAGAAAATAAAACACGATTCTACCAAGCCA
GCACGAGCGAATTGTATGGTGAGGTCTTAGAAACCCCACAAAATGAAAACACCCCCTTTAACCCACGAAGCCCCTATGCG
GTCGCTAAAATGTATGCCTTTTACATCACTAAAAATTACAGAGAAGCCTATAACTTGTTTGCGGTTAATGGCATTCTTTT
TAACCATGAAAGTAGGGTGAGAGGCGAAACTTTTGTCACTCGTAAAATCACACGAGCCGCAAGCGCGATAGCGTATAATT
TAACGGATTGCTTGTATTTAGGGAATTTAGATGCTAAAAGAGACTGGGGGCATGCCAAAGATTATGTGAAAATGATGCAT
TTAATGCTCCAAGCGCCCACTCCGCAAGACTATGTGATCGCCACAGGAAAAACCACAAGCGTGCGCGATTTTGTGAAAAT
GAGCTTTGAATTTATCGGTATCAATTTAGAATTTCAAAATACAGGGATTAAAGAAATTGGTTTGATTGAAAGTATTGATG
AAAAAAGAGCGAACACTTTAAATCTAAACTTAAGCCATTTAAAAACAGGCAAAATCGTGGTGCGTATAGATGAACGCTAC
TTTAGGCCTACTGAAGTGGATTTGCTCTTAGGCGATCCCACTAAGGCTGAGAAAGAGCTAGGCTGGGTGAGAGAATACGA
TTTGAAAGAGTTGGTTAAGGACATGTTAGAGCATGATTTAAGCGAATGCCAGAAAAACTTTTACTTACAAGATGGGGGCT
ATACTTTAAGGAATTTTTATGAATGA

Upstream 100 bases:

>100_bases
TATCATAGAAGTCCAAGTGGGCGAGTATGTCGGTGAAGACGATATTGTAAGGGTTGATGATGATTTTAACAGACAAAATC
CGAGTAAATAAAGGACAATA

Downstream 100 bases:

>100_bases
GATTATTTTAATCACCGGTGCCTATGGCATGGTGGGGCAAAACACGGCGTTGTATTTTAAAAAAAATAAGCCTGATATCA
CTTTACTCACCCCTAAAAGA

Product: hypothetical protein

Products: NA

Alternate protein names: GDP-D-mannose dehydratase; ORF13.7 [H]

Number of amino acids: Translated: 381; Mature: 381

Protein sequence:

>381_residues
MKEKIALITGVTGQDGSYLTEYLLNLGYEVHGLKRRSSSINTSRIDHLYEDLHSEHKRRFFLHYGDMTDSSNLIHLIATT
KPTEIYNLAAQSHVKVSFETPEYTANADGIGTLRILESMRILGLENKTRFYQASTSELYGEVLETPQNENTPFNPRSPYA
VAKMYAFYITKNYREAYNLFAVNGILFNHESRVRGETFVTRKITRAASAIAYNLTDCLYLGNLDAKRDWGHAKDYVKMMH
LMLQAPTPQDYVIATGKTTSVRDFVKMSFEFIGINLEFQNTGIKEIGLIESIDEKRANTLNLNLSHLKTGKIVVRIDERY
FRPTEVDLLLGDPTKAEKELGWVREYDLKELVKDMLEHDLSECQKNFYLQDGGYTLRNFYE

Sequences:

>Translated_381_residues
MKEKIALITGVTGQDGSYLTEYLLNLGYEVHGLKRRSSSINTSRIDHLYEDLHSEHKRRFFLHYGDMTDSSNLIHLIATT
KPTEIYNLAAQSHVKVSFETPEYTANADGIGTLRILESMRILGLENKTRFYQASTSELYGEVLETPQNENTPFNPRSPYA
VAKMYAFYITKNYREAYNLFAVNGILFNHESRVRGETFVTRKITRAASAIAYNLTDCLYLGNLDAKRDWGHAKDYVKMMH
LMLQAPTPQDYVIATGKTTSVRDFVKMSFEFIGINLEFQNTGIKEIGLIESIDEKRANTLNLNLSHLKTGKIVVRIDERY
FRPTEVDLLLGDPTKAEKELGWVREYDLKELVKDMLEHDLSECQKNFYLQDGGYTLRNFYE
>Mature_381_residues
MKEKIALITGVTGQDGSYLTEYLLNLGYEVHGLKRRSSSINTSRIDHLYEDLHSEHKRRFFLHYGDMTDSSNLIHLIATT
KPTEIYNLAAQSHVKVSFETPEYTANADGIGTLRILESMRILGLENKTRFYQASTSELYGEVLETPQNENTPFNPRSPYA
VAKMYAFYITKNYREAYNLFAVNGILFNHESRVRGETFVTRKITRAASAIAYNLTDCLYLGNLDAKRDWGHAKDYVKMMH
LMLQAPTPQDYVIATGKTTSVRDFVKMSFEFIGINLEFQNTGIKEIGLIESIDEKRANTLNLNLSHLKTGKIVVRIDERY
FRPTEVDLLLGDPTKAEKELGWVREYDLKELVKDMLEHDLSECQKNFYLQDGGYTLRNFYE

Specific function: Biosynthesis of the slime polysaccharide colanic acid. First of the three steps in the biosynthesis of GDP-fucose from GDP-mannose. [C]

COG id: COG1089

COG function: function code M; GDP-D-mannose dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GDP-mannose 4,6-dehydratase family [H]

Homologues:

Organism=Homo sapiens, GI4504031, Length=362, Percent_Identity=54.9723756906077, Blast_Score=407, Evalue=1e-114,
Organism=Escherichia coli, GI1788366, Length=371, Percent_Identity=59.2991913746631, Blast_Score=465, Evalue=1e-132,
Organism=Escherichia coli, GI48994969, Length=379, Percent_Identity=22.9551451187335, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1788353, Length=257, Percent_Identity=22.568093385214, Blast_Score=64, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17539424, Length=358, Percent_Identity=56.145251396648, Blast_Score=409, Evalue=1e-115,
Organism=Caenorhabditis elegans, GI17539422, Length=358, Percent_Identity=56.145251396648, Blast_Score=409, Evalue=1e-114,
Organism=Caenorhabditis elegans, GI133901790, Length=358, Percent_Identity=56.145251396648, Blast_Score=409, Evalue=1e-114,
Organism=Caenorhabditis elegans, GI133901786, Length=358, Percent_Identity=56.145251396648, Blast_Score=409, Evalue=1e-114,
Organism=Caenorhabditis elegans, GI133901788, Length=358, Percent_Identity=56.145251396648, Blast_Score=409, Evalue=1e-114,
Organism=Caenorhabditis elegans, GI17507723, Length=359, Percent_Identity=56.2674094707521, Blast_Score=406, Evalue=1e-114,
Organism=Drosophila melanogaster, GI24158427, Length=365, Percent_Identity=57.2602739726027, Blast_Score=433, Evalue=1e-122,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR006368
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.47 [H]

Molecular weight: Translated: 43860; Mature: 43860

Theoretical pI: Translated: 6.66; Mature: 6.66

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKEKIALITGVTGQDGSYLTEYLLNLGYEVHGLKRRSSSINTSRIDHLYEDLHSEHKRRF
CCCCEEEEEECCCCCCHHHHHHHHHCCCEEECHHHHCCCCCHHHHHHHHHHHHHHHCEEE
FLHYGDMTDSSNLIHLIATTKPTEIYNLAAQSHVKVSFETPEYTANADGIGTLRILESMR
EEEECCCCCCCCEEEEEEECCCCHHHHHHCCCEEEEEEECCCCCCCCCCCHHHHHHHHHH
ILGLENKTRFYQASTSELYGEVLETPQNENTPFNPRSPYAVAKMYAFYITKNYREAYNLF
HCCCCCCCEEEECCHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHCHHHHHHEE
AVNGILFNHESRVRGETFVTRKITRAASAIAYNLTDCLYLGNLDAKRDWGHAKDYVKMMH
EEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCHHHHHHHHHH
LMLQAPTPQDYVIATGKTTSVRDFVKMSFEFIGINLEFQNTGIKEIGLIESIDEKRANTL
HHHCCCCCCCEEEEECCCCHHHHHHHHEEEEEEEEEEECCCCCCHHHHHHHHHHHCCCEE
NLNLSHLKTGKIVVRIDERYFRPTEVDLLLGDPTKAEKELGWVREYDLKELVKDMLEHDL
EEEHHEECCCEEEEEECCCCCCCCEEEEEECCCCCHHHHHCCHHHCCHHHHHHHHHHHHH
SECQKNFYLQDGGYTLRNFYE
HHHHHCCEEECCCEEHHCCCC
>Mature Secondary Structure
MKEKIALITGVTGQDGSYLTEYLLNLGYEVHGLKRRSSSINTSRIDHLYEDLHSEHKRRF
CCCCEEEEEECCCCCCHHHHHHHHHCCCEEECHHHHCCCCCHHHHHHHHHHHHHHHCEEE
FLHYGDMTDSSNLIHLIATTKPTEIYNLAAQSHVKVSFETPEYTANADGIGTLRILESMR
EEEECCCCCCCCEEEEEEECCCCHHHHHHCCCEEEEEEECCCCCCCCCCCHHHHHHHHHH
ILGLENKTRFYQASTSELYGEVLETPQNENTPFNPRSPYAVAKMYAFYITKNYREAYNLF
HCCCCCCCEEEECCHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHCHHHHHHEE
AVNGILFNHESRVRGETFVTRKITRAASAIAYNLTDCLYLGNLDAKRDWGHAKDYVKMMH
EEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCHHHHHHHHHH
LMLQAPTPQDYVIATGKTTSVRDFVKMSFEFIGINLEFQNTGIKEIGLIESIDEKRANTL
HHHCCCCCCCEEEEECCCCHHHHHHHHEEEEEEEEEEECCCCCCHHHHHHHHHHHCCCEE
NLNLSHLKTGKIVVRIDERYFRPTEVDLLLGDPTKAEKELGWVREYDLKELVKDMLEHDL
EEEHHEECCCEEEEEECCCCCCCCEEEEEECCCCCHHHHHCCHHHCCHHHHHHHHHHHHH
SECQKNFYLQDGGYTLRNFYE
HHHHHCCEEECCCEEHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8932701 [H]