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The map label for this gene is wbcJ [H]

Identifier: 109946690

GI number: 109946690

Start: 54619

End: 55551

Strand: Direct

Name: wbcJ [H]

Synonym: Hac_0057

Alternate gene names: 109946690

Gene position: 54619-55551 (Clockwise)

Preceding gene: 109946689

Following gene: 109946694

Centisome position: 3.51

GC content: 39.55

Gene sequence:

>933_bases
ATGAATGAGATTATTTTAATCACCGGTGCCTATGGCATGGTGGGGCAAAACACGGCGTTGTATTTTAAAAAAAATAAGCC
TGATATCACTTTACTCACCCCTAAAAGAAGCGAATTAAACCTGCTAGACAAAGACAATGTTCAAGCCTATTTGAAAGAAT
ACAAGCCGATAGGCATTATCCATTGTGCTGGGAGAGTGGGGGGCATTGTCGCTAACATGAACGATCTTTCAGCTTACATG
GTTGAAAACTTGCTCATGGGCTTGTATCTCTTTTCTAGCGCTTTAGATTTGGGTATCAAAAAAGCCATCAATTTAGCGAG
CTCTTGTGCTTATCCTAAATTCGCCCCTAACCCTTTAAAAGAGAGCGATTTGTTAAACGGCTCTTTAGAGCCAACCAATG
AAGGCTACGCTTTAGCCAAACTCTCTGTGATGAAATATTGCGAATATGTGAGCGCTGAAAAGGGCGTTTTTTATAAAACC
TTAGTGCCTTGCAACCTTTATGGAGAGTTTGACAAATTTGAAGAAAAAATAGCGCACATGATACCAGGGCTTATTTCTAG
GATGCACACCGCCAAATTAAAGGGCGAAAAAAATTTTGTGATGTGGGGCGATGGAACCGCTAGAAGAGAATATCTAAACG
CTAAAGATTTAGCCCGATTTATTGCTCTAGCTTATGATAATATCGCTCAAATCCCTAGCGTGATGAATGTAGGCTCTGGC
GTGGATTATAGCATTGAAGAGTATTATGAAATGGTCGCTCAGGTTTTAGACTATAAAGGCGTGTTTGTTAAAGACTTGTC
TAAGCCGGTGGGCATGCAGCAAAAACTTATGGATATTTCCAAACAAAAGGCTTTAAAATGGGAATTAGAAATCTCTTTAG
AGCAAGGCATCAAAGAAGCTTATGAGTATTATTTGAAGCTTTTAGGAGCTTAA

Upstream 100 bases:

>100_bases
GATTTGAAAGAGTTGGTTAAGGACATGTTAGAGCATGATTTAAGCGAATGCCAGAAAAACTTTTACTTACAAGATGGGGG
CTATACTTTAAGGAATTTTT

Downstream 100 bases:

>100_bases
AATAATGGTTTTGACGCTTTATTTATAAAGTCCATGCTATGCTCATAGTGCTTTGATATAACAATCTATTCCTTTTTAAA
TGGATGCTAGCGATCTAGTC

Product: GDP-fucose synthetase

Products: GDP-4-dehydro-6-deoxy-D-mannose; NADPH; H+

Alternate protein names: NA

Number of amino acids: Translated: 310; Mature: 310

Protein sequence:

>310_residues
MNEIILITGAYGMVGQNTALYFKKNKPDITLLTPKRSELNLLDKDNVQAYLKEYKPIGIIHCAGRVGGIVANMNDLSAYM
VENLLMGLYLFSSALDLGIKKAINLASSCAYPKFAPNPLKESDLLNGSLEPTNEGYALAKLSVMKYCEYVSAEKGVFYKT
LVPCNLYGEFDKFEEKIAHMIPGLISRMHTAKLKGEKNFVMWGDGTARREYLNAKDLARFIALAYDNIAQIPSVMNVGSG
VDYSIEEYYEMVAQVLDYKGVFVKDLSKPVGMQQKLMDISKQKALKWELEISLEQGIKEAYEYYLKLLGA

Sequences:

>Translated_310_residues
MNEIILITGAYGMVGQNTALYFKKNKPDITLLTPKRSELNLLDKDNVQAYLKEYKPIGIIHCAGRVGGIVANMNDLSAYM
VENLLMGLYLFSSALDLGIKKAINLASSCAYPKFAPNPLKESDLLNGSLEPTNEGYALAKLSVMKYCEYVSAEKGVFYKT
LVPCNLYGEFDKFEEKIAHMIPGLISRMHTAKLKGEKNFVMWGDGTARREYLNAKDLARFIALAYDNIAQIPSVMNVGSG
VDYSIEEYYEMVAQVLDYKGVFVKDLSKPVGMQQKLMDISKQKALKWELEISLEQGIKEAYEYYLKLLGA
>Mature_310_residues
MNEIILITGAYGMVGQNTALYFKKNKPDITLLTPKRSELNLLDKDNVQAYLKEYKPIGIIHCAGRVGGIVANMNDLSAYM
VENLLMGLYLFSSALDLGIKKAINLASSCAYPKFAPNPLKESDLLNGSLEPTNEGYALAKLSVMKYCEYVSAEKGVFYKT
LVPCNLYGEFDKFEEKIAHMIPGLISRMHTAKLKGEKNFVMWGDGTARREYLNAKDLARFIALAYDNIAQIPSVMNVGSG
VDYSIEEYYEMVAQVLDYKGVFVKDLSKPVGMQQKLMDISKQKALKWELEISLEQGIKEAYEYYLKLLGA

Specific function: Probable nucleotide sugar epimerase/dehydrogenase [H]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fucose synthase family [H]

Homologues:

Organism=Homo sapiens, GI4507709, Length=304, Percent_Identity=25.9868421052632, Blast_Score=129, Evalue=3e-30,
Organism=Escherichia coli, GI1788365, Length=310, Percent_Identity=37.4193548387097, Blast_Score=219, Evalue=3e-58,
Organism=Caenorhabditis elegans, GI17554428, Length=305, Percent_Identity=24.2622950819672, Blast_Score=109, Evalue=2e-24,
Organism=Drosophila melanogaster, GI19922778, Length=311, Percent_Identity=27.0096463022508, Blast_Score=139, Evalue=2e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: 1.1.1.271

Molecular weight: Translated: 34736; Mature: 34736

Theoretical pI: Translated: 7.37; Mature: 7.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNEIILITGAYGMVGQNTALYFKKNKPDITLLTPKRSELNLLDKDNVQAYLKEYKPIGII
CCCEEEEEECCCCCCCCEEEEEECCCCCEEEECCCCCCCCCCCCCHHHHHHHHCCCCEEE
HCAGRVGGIVANMNDLSAYMVENLLMGLYLFSSALDLGIKKAINLASSCAYPKFAPNPLK
EECCCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
ESDLLNGSLEPTNEGYALAKLSVMKYCEYVSAEKGVFYKTLVPCNLYGEFDKFEEKIAHM
CHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCHHHHHHHHHHH
IPGLISRMHTAKLKGEKNFVMWGDGTARREYLNAKDLARFIALAYDNIAQIPSVMNVGSG
HHHHHHHHHHHHCCCCCCEEEECCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCC
VDYSIEEYYEMVAQVLDYKGVFVKDLSKPVGMQQKLMDISKQKALKWELEISLEQGIKEA
CCCCHHHHHHHHHHHHCCCCHHHHHHCCCCCHHHHHHHHHHHHHEEEEEEEEHHHHHHHH
YEYYLKLLGA
HHHHHHHHCC
>Mature Secondary Structure
MNEIILITGAYGMVGQNTALYFKKNKPDITLLTPKRSELNLLDKDNVQAYLKEYKPIGII
CCCEEEEEECCCCCCCCEEEEEECCCCCEEEECCCCCCCCCCCCCHHHHHHHHCCCCEEE
HCAGRVGGIVANMNDLSAYMVENLLMGLYLFSSALDLGIKKAINLASSCAYPKFAPNPLK
EECCCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
ESDLLNGSLEPTNEGYALAKLSVMKYCEYVSAEKGVFYKTLVPCNLYGEFDKFEEKIAHM
CHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCHHHHHHHHHHH
IPGLISRMHTAKLKGEKNFVMWGDGTARREYLNAKDLARFIALAYDNIAQIPSVMNVGSG
HHHHHHHHHHHHCCCCCCEEEECCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCC
VDYSIEEYYEMVAQVLDYKGVFVKDLSKPVGMQQKLMDISKQKALKWELEISLEQGIKEA
CCCCHHHHHHHHHHHHCCCCHHHHHHCCCCCHHHHHHHHHHHHHEEEEEEEEHHHHHHHH
YEYYLKLLGA
HHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GDP-L-fucose; NADP+

Specific reaction: GDP-L-fucose + NADP+ = GDP-4-dehydro-6-deoxy-D-mannose + NADPH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1472718 [H]