BacMap

Definition	Roseobacter denitrificans OCh 114, complete genome.
Accession	NC_008209
Length	4,133,097

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The map label for this gene is gno [H]

Identifier: 110680952

GI number: 110680952

Start: 3682174

End: 3682926

Strand: Direct

Name: gno [H]

Synonym: RD1_3809

Alternate gene names: 110680952

Gene position: 3682174-3682926 (Clockwise)

Preceding gene: 110680951

Following gene: 110680953

Centisome position: 89.09

GC content: 61.22

Gene sequence:

>753_bases
GTGGCGGCGCTCTTTGATGTCAGCGGCAGGGTGGTTTGCGTGACCGGCGCAAGCAGCGGCCTCGGTCGACAGGCCGCCAC
GATGCTGGCACAGGCCGGCGCCAAAGTCATCGGCGTCGCCCGACGCGCAGAAGAGCTTGAAGCCTGGAACAACTCAACCG
AGGGACAGACCGCCTACGTCACCGCCGACCTGGCCGACAGGGCGGCATTGAAAAAGGTGGCCGTGCAGGTCAGCATGCCC
TTTGGCACGGTCGATGTCCTGATCAACGCGGCGGGTATCAATACGCGCCAACACGCCGACGAGGTGACAGACACCGGATG
GGATGTAACCCTCAATCTGAACCTCGCCGCGCCTTTCTTTCTCGCGCAAGCGCTGGTTCCCGCCATGAAACGGAAAGGAT
GGGGGCGGATCATAAATTTCGCCTCACTGCAATCGCAGCGCGCCTTTCCCAATGGCATCAGCTATGGCGCGTCCAAAGGC
GGGGTGGAACAGATGACCCGTGCCATGGCCGAAGCATGGTCAGGCTATGGCATCACCGCGAACGCCGTGGCACCCGGTTT
TTTCCGCACGGAACTCACGGGGCCGGTTTTTGCCGATCCCGAACTGGCGCAGCGCAATGCGGATCAGACCTGCATCGGGC
GCAACGGCGAACCTGTGGACCTTGATGGACCACTGATGTTTTTCTGCGCGCCCGCGTCTGACTATGTGACTGGCCAGACA
CTATTTGTCGACGGAGGTTTTACAGCAAAATGA

Upstream 100 bases:

>100_bases
GTCTGGAGGGGATGGAAGGGCACGCGCGCGCCGCGGATGCGCGCCTGCACAAGTATTTTCCGAACGAGAACTTTGACCTC
TCAGCGGTGGATTAAGTCAG

Downstream 100 bases:

>100_bases
AGGCGTTAGTCTATACTGACATTGAGCAATTGACCTATCAGGACCGCCCCCTCCCAACCCCCGGCGCGGGCGAGCATCTG
GTGCGCATCGAGGCGTCGGG

Product: gluconate 5-dehydrogenase

Products: NA

Alternate protein names: 2-keto-3-deoxygluconate 5-dehydrogenase; 2-keto-3-deoxygluconate oxidoreductase; KDG oxidoreductase [H]

Number of amino acids: Translated: 250; Mature: 249

Protein sequence:

>250_residues
MAALFDVSGRVVCVTGASSGLGRQAATMLAQAGAKVIGVARRAEELEAWNNSTEGQTAYVTADLADRAALKKVAVQVSMP
FGTVDVLINAAGINTRQHADEVTDTGWDVTLNLNLAAPFFLAQALVPAMKRKGWGRIINFASLQSQRAFPNGISYGASKG
GVEQMTRAMAEAWSGYGITANAVAPGFFRTELTGPVFADPELAQRNADQTCIGRNGEPVDLDGPLMFFCAPASDYVTGQT
LFVDGGFTAK

Sequences:

>Translated_250_residues
MAALFDVSGRVVCVTGASSGLGRQAATMLAQAGAKVIGVARRAEELEAWNNSTEGQTAYVTADLADRAALKKVAVQVSMP
FGTVDVLINAAGINTRQHADEVTDTGWDVTLNLNLAAPFFLAQALVPAMKRKGWGRIINFASLQSQRAFPNGISYGASKG
GVEQMTRAMAEAWSGYGITANAVAPGFFRTELTGPVFADPELAQRNADQTCIGRNGEPVDLDGPLMFFCAPASDYVTGQT
LFVDGGFTAK
>Mature_249_residues
AALFDVSGRVVCVTGASSGLGRQAATMLAQAGAKVIGVARRAEELEAWNNSTEGQTAYVTADLADRAALKKVAVQVSMPF
GTVDVLINAAGINTRQHADEVTDTGWDVTLNLNLAAPFFLAQALVPAMKRKGWGRIINFASLQSQRAFPNGISYGASKGG
VEQMTRAMAEAWSGYGITANAVAPGFFRTELTGPVFADPELAQRNADQTCIGRNGEPVDLDGPLMFFCAPASDYVTGQTL
FVDGGFTAK

Specific function: Catalyzes the reduction of 2,5-diketo-3-deoxygluconate (DKII or 4,6-dihydroxy-2,5-dioxohexanoate) into 2-keto-3- deoxygluconate (KDG or 2-dehydro-3-deoxygluconate) with a concomitant oxidation of NADH [H]

COG id: COG1028

COG function: function code IQR; Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI32483357, Length=251, Percent_Identity=28.6852589641434, Blast_Score=115, Evalue=3e-26,
Organism=Homo sapiens, GI7705925, Length=242, Percent_Identity=31.404958677686, Blast_Score=106, Evalue=2e-23,
Organism=Homo sapiens, GI19923817, Length=250, Percent_Identity=23.2, Blast_Score=99, Evalue=4e-21,
Organism=Homo sapiens, GI20149619, Length=195, Percent_Identity=32.3076923076923, Blast_Score=98, Evalue=8e-21,
Organism=Homo sapiens, GI40254992, Length=238, Percent_Identity=28.9915966386555, Blast_Score=96, Evalue=3e-20,
Organism=Homo sapiens, GI126723750, Length=251, Percent_Identity=26.6932270916335, Blast_Score=91, Evalue=1e-18,
Organism=Homo sapiens, GI15277342, Length=246, Percent_Identity=29.6747967479675, Blast_Score=88, Evalue=6e-18,
Organism=Homo sapiens, GI5031737, Length=246, Percent_Identity=26.8292682926829, Blast_Score=86, Evalue=3e-17,
Organism=Homo sapiens, GI225579078, Length=234, Percent_Identity=29.9145299145299, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI59889578, Length=193, Percent_Identity=29.5336787564767, Blast_Score=78, Evalue=6e-15,
Organism=Homo sapiens, GI31542939, Length=210, Percent_Identity=30.4761904761905, Blast_Score=78, Evalue=6e-15,
Organism=Homo sapiens, GI157739938, Length=197, Percent_Identity=26.3959390862944, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI66933014, Length=247, Percent_Identity=27.5303643724696, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI7706318, Length=193, Percent_Identity=28.4974093264249, Blast_Score=75, Evalue=7e-14,
Organism=Homo sapiens, GI10190704, Length=246, Percent_Identity=24.390243902439, Blast_Score=70, Evalue=1e-12,
Organism=Homo sapiens, GI186928839, Length=203, Percent_Identity=28.5714285714286, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI226371731, Length=201, Percent_Identity=24.3781094527363, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI210032110, Length=196, Percent_Identity=27.0408163265306, Blast_Score=69, Evalue=3e-12,
Organism=Homo sapiens, GI209862901, Length=213, Percent_Identity=29.5774647887324, Blast_Score=68, Evalue=7e-12,
Organism=Homo sapiens, GI19923983, Length=213, Percent_Identity=29.5774647887324, Blast_Score=68, Evalue=7e-12,
Organism=Homo sapiens, GI166795268, Length=201, Percent_Identity=29.3532338308458, Blast_Score=68, Evalue=7e-12,
Organism=Homo sapiens, GI142976729, Length=213, Percent_Identity=25.8215962441315, Blast_Score=68, Evalue=7e-12,
Organism=Homo sapiens, GI109715829, Length=212, Percent_Identity=28.3018867924528, Blast_Score=68, Evalue=9e-12,
Organism=Homo sapiens, GI7657478, Length=201, Percent_Identity=28.8557213930348, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI126723191, Length=190, Percent_Identity=25.7894736842105, Blast_Score=66, Evalue=3e-11,
Organism=Homo sapiens, GI4504505, Length=194, Percent_Identity=27.319587628866, Blast_Score=66, Evalue=3e-11,
Organism=Homo sapiens, GI10190746, Length=216, Percent_Identity=28.7037037037037, Blast_Score=65, Evalue=5e-11,
Organism=Homo sapiens, GI224922801, Length=174, Percent_Identity=31.6091954022989, Blast_Score=65, Evalue=8e-11,
Organism=Escherichia coli, GI1789208, Length=252, Percent_Identity=38.4920634920635, Blast_Score=152, Evalue=1e-38,
Organism=Escherichia coli, GI1790717, Length=251, Percent_Identity=32.6693227091633, Blast_Score=135, Evalue=2e-33,
Organism=Escherichia coli, GI87082160, Length=249, Percent_Identity=31.3253012048193, Blast_Score=124, Evalue=4e-30,
Organism=Escherichia coli, GI1787335, Length=243, Percent_Identity=32.5102880658436, Blast_Score=124, Evalue=7e-30,
Organism=Escherichia coli, GI1788459, Length=244, Percent_Identity=28.6885245901639, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI1787905, Length=243, Percent_Identity=29.6296296296296, Blast_Score=92, Evalue=2e-20,
Organism=Escherichia coli, GI87082100, Length=252, Percent_Identity=25.3968253968254, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI1786812, Length=252, Percent_Identity=26.984126984127, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI1787820, Length=178, Percent_Identity=25.8426966292135, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1789378, Length=248, Percent_Identity=27.8225806451613, Blast_Score=75, Evalue=3e-15,
Organism=Escherichia coli, GI2367365, Length=243, Percent_Identity=27.9835390946502, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI2367175, Length=245, Percent_Identity=25.7142857142857, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1789057, Length=256, Percent_Identity=27.34375, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1787526, Length=211, Percent_Identity=28.436018957346, Blast_Score=67, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI115534694, Length=249, Percent_Identity=29.3172690763052, Blast_Score=115, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI17561402, Length=251, Percent_Identity=28.6852589641434, Blast_Score=107, Evalue=4e-24,
Organism=Caenorhabditis elegans, GI17531453, Length=258, Percent_Identity=30.6201550387597, Blast_Score=101, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI17560676, Length=253, Percent_Identity=26.4822134387352, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17559104, Length=260, Percent_Identity=30.7692307692308, Blast_Score=99, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17562910, Length=263, Percent_Identity=28.1368821292776, Blast_Score=99, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI71994604, Length=249, Percent_Identity=31.3253012048193, Blast_Score=98, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI17562904, Length=258, Percent_Identity=29.4573643410853, Blast_Score=96, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI25147288, Length=258, Percent_Identity=27.906976744186, Blast_Score=96, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17562906, Length=260, Percent_Identity=28.8461538461538, Blast_Score=96, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17563726, Length=267, Percent_Identity=29.9625468164794, Blast_Score=94, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI17562908, Length=261, Percent_Identity=30.6513409961686, Blast_Score=93, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17560220, Length=260, Percent_Identity=29.2307692307692, Blast_Score=92, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI193204405, Length=259, Percent_Identity=30.8880308880309, Blast_Score=90, Evalue=9e-19,
Organism=Caenorhabditis elegans, GI17562990, Length=263, Percent_Identity=28.5171102661597, Blast_Score=87, Evalue=8e-18,
Organism=Caenorhabditis elegans, GI17560332, Length=265, Percent_Identity=29.811320754717, Blast_Score=87, Evalue=9e-18,
Organism=Caenorhabditis elegans, GI72000259, Length=258, Percent_Identity=28.6821705426357, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17565030, Length=261, Percent_Identity=30.6513409961686, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17560150, Length=265, Percent_Identity=27.9245283018868, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17555706, Length=244, Percent_Identity=27.4590163934426, Blast_Score=84, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI71994600, Length=250, Percent_Identity=28.4, Blast_Score=83, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17551412, Length=260, Percent_Identity=27.6923076923077, Blast_Score=82, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17564282, Length=240, Percent_Identity=29.1666666666667, Blast_Score=81, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17544670, Length=265, Percent_Identity=26.7924528301887, Blast_Score=81, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI17538480, Length=264, Percent_Identity=27.2727272727273, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17536651, Length=250, Percent_Identity=26.4, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17536025, Length=249, Percent_Identity=25.7028112449799, Blast_Score=77, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI17538486, Length=257, Percent_Identity=28.0155642023346, Blast_Score=73, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI71982365, Length=193, Percent_Identity=25.9067357512953, Blast_Score=73, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17558006, Length=207, Percent_Identity=27.536231884058, Blast_Score=73, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI193203117, Length=165, Percent_Identity=31.5151515151515, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI193203115, Length=163, Percent_Identity=31.9018404907975, Blast_Score=72, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI17561272, Length=249, Percent_Identity=28.5140562248996, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17538182, Length=268, Percent_Identity=29.4776119402985, Blast_Score=69, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI32563809, Length=209, Percent_Identity=26.7942583732057, Blast_Score=65, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17568967, Length=199, Percent_Identity=26.6331658291457, Blast_Score=65, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17508895, Length=194, Percent_Identity=25.2577319587629, Blast_Score=65, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI17507613, Length=209, Percent_Identity=26.7942583732057, Blast_Score=65, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6323882, Length=197, Percent_Identity=26.9035532994924, Blast_Score=72, Evalue=6e-14,
Organism=Saccharomyces cerevisiae, GI6322861, Length=249, Percent_Identity=26.9076305220884, Blast_Score=72, Evalue=6e-14,
Organism=Drosophila melanogaster, GI23397609, Length=247, Percent_Identity=32.3886639676113, Blast_Score=115, Evalue=3e-26,
Organism=Drosophila melanogaster, GI24644339, Length=250, Percent_Identity=30, Blast_Score=110, Evalue=8e-25,
Organism=Drosophila melanogaster, GI24643142, Length=248, Percent_Identity=28.2258064516129, Blast_Score=103, Evalue=8e-23,
Organism=Drosophila melanogaster, GI21358495, Length=195, Percent_Identity=33.3333333333333, Blast_Score=99, Evalue=2e-21,
Organism=Drosophila melanogaster, GI28571526, Length=245, Percent_Identity=30.6122448979592, Blast_Score=99, Evalue=3e-21,
Organism=Drosophila melanogaster, GI24639444, Length=246, Percent_Identity=27.6422764227642, Blast_Score=98, Evalue=4e-21,
Organism=Drosophila melanogaster, GI21357041, Length=251, Percent_Identity=29.0836653386454, Blast_Score=98, Evalue=5e-21,
Organism=Drosophila melanogaster, GI21355319, Length=252, Percent_Identity=25, Blast_Score=91, Evalue=6e-19,
Organism=Drosophila melanogaster, GI24644337, Length=250, Percent_Identity=32, Blast_Score=91, Evalue=9e-19,
Organism=Drosophila melanogaster, GI24584744, Length=199, Percent_Identity=28.1407035175879, Blast_Score=75, Evalue=4e-14,
Organism=Drosophila melanogaster, GI28574402, Length=199, Percent_Identity=28.1407035175879, Blast_Score=75, Evalue=4e-14,
Organism=Drosophila melanogaster, GI18859837, Length=189, Percent_Identity=28.5714285714286, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI161076933, Length=185, Percent_Identity=28.1081081081081, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24584748, Length=185, Percent_Identity=28.1081081081081, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI45553409, Length=199, Percent_Identity=26.1306532663317, Blast_Score=71, Evalue=6e-13,
Organism=Drosophila melanogaster, GI24647948, Length=199, Percent_Identity=26.1306532663317, Blast_Score=71, Evalue=6e-13,
Organism=Drosophila melanogaster, GI161076371, Length=217, Percent_Identity=27.1889400921659, Blast_Score=71, Evalue=6e-13,
Organism=Drosophila melanogaster, GI22024069, Length=204, Percent_Identity=28.4313725490196, Blast_Score=71, Evalue=7e-13,
Organism=Drosophila melanogaster, GI24647946, Length=199, Percent_Identity=26.1306532663317, Blast_Score=71, Evalue=8e-13,
Organism=Drosophila melanogaster, GI24586328, Length=202, Percent_Identity=27.2277227722772, Blast_Score=69, Evalue=4e-12,
Organism=Drosophila melanogaster, GI21357673, Length=191, Percent_Identity=24.6073298429319, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI19921754, Length=206, Percent_Identity=27.1844660194175, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI28573296, Length=206, Percent_Identity=27.6699029126214, Blast_Score=66, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011286
- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040
- InterPro:   IPR020904 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: =1.1.1.127 [H]

Molecular weight: Translated: 26301; Mature: 26170

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAALFDVSGRVVCVTGASSGLGRQAATMLAQAGAKVIGVARRAEELEAWNNSTEGQTAYV
CCEEEECCCCEEEEECCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCEEEE
TADLADRAALKKVAVQVSMPFGTVDVLINAAGINTRQHADEVTDTGWDVTLNLNLAAPFF
EECHHHHHHHHHHEEEEECCCCEEEEEEECCCCCCHHHHHHHCCCCCEEEEEECCCCHHH
LAQALVPAMKRKGWGRIINFASLQSQRAFPNGISYGASKGGVEQMTRAMAEAWSGYGITA
HHHHHHHHHHCCCCHHEEEHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEE
NAVAPGFFRTELTGPVFADPELAQRNADQTCIGRNGEPVDLDGPLMFFCAPASDYVTGQT
CCCCCCEEECCCCCCCCCCHHHHHCCCCCCEECCCCCEEECCCCEEEEEECCCCCCCCCE
LFVDGGFTAK
EEECCCCCCC
>Mature Secondary Structure 
AALFDVSGRVVCVTGASSGLGRQAATMLAQAGAKVIGVARRAEELEAWNNSTEGQTAYV
CEEEECCCCEEEEECCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCEEEE
TADLADRAALKKVAVQVSMPFGTVDVLINAAGINTRQHADEVTDTGWDVTLNLNLAAPFF
EECHHHHHHHHHHEEEEECCCCEEEEEEECCCCCCHHHHHHHCCCCCEEEEEECCCCHHH
LAQALVPAMKRKGWGRIINFASLQSQRAFPNGISYGASKGGVEQMTRAMAEAWSGYGITA
HHHHHHHHHHCCCCHHEEEHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEE
NAVAPGFFRTELTGPVFADPELAQRNADQTCIGRNGEPVDLDGPLMFFCAPASDYVTGQT
CCCCCCEEECCCCCCCCCCHHHHHCCCCCCEECCCCCEEECCCCEEEEEECCCCCCCCCE
LFVDGGFTAK
EEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1766386 [H]