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The map label for this gene is holB [H]

Identifier: 110680631

GI number: 110680631

Start: 3337115

End: 3338221

Strand: Direct

Name: holB [H]

Synonym: RD1_3467

Alternate gene names: 110680631

Gene position: 3337115-3338221 (Clockwise)

Preceding gene: 110680630

Following gene: 110680632

Centisome position: 80.74

GC content: 65.04

Gene sequence:

>1107_bases
ATGACGGCCCCCGATCAAATTGAAGGCGCGCCGCACCCGCGCGACACGCCGCAGGTTTTTGGTCAAGCCACTGCCGAACA
GGGGTTTCTGGACGCATATGCAACGGGACGCCTGCATCATGCATGGTTGCTCACCGGGCCGCGCGGTGTGGGCAAGGCGA
CGCTGGCATGGGCGATCGCGCGGTTTTTGCTGGCCACACCACCGCAAGAGGATGAGGGCCTCTTCGGTGCGCCACCCGCC
CCCACAACGCTCGCGATCGACCCCGAACATCCCGTTGCACGACGGATTGCGGCCGGCGCAGAGCCCGGCCTGAAACATAT
CACACGGTCCGAAAACGAGAAAACCGGGCGCATGCGTGACGTGATCGCCGTCGATGACATCCGCGGCCTGTCGTCGTTTT
TTCAACTCTCTGCCACGGAAGGCGGGCGGCGGGTTGTCATCGTGGATGCTGCGGATGAGATGAACCCGCAGGCCGCAAAC
GCCCTGTTGAAGATGCTTGAGGAACCCCCGGCGCGCGCCACGTTGATCCTTATCAGCCATCAACCCTCCGGCCTTTTGCC
AACGATCCGCTCGCGGTGCCGGGTATTGCGCCTCGGCACACTGCAGCCGGAGGATATGGAAAAAGCGCTCACGCAGGCAG
GAATTGATCCCGCCGGGCATGGGCCTGCACTGGCCGAGTTATCAGGCGGGTCGGTCGGGGCCGCCGTGCGGCTGTCAAAC
TTCGATGGCCTGAAAACATATGCGGAGCTTGTTCAACTGATCATCGCGCTGCCGCAGTTCGACCGGGCGCGCGCGTTGAA
ACTGGCCGAAGCAGCCGCCGCGCGCGGGGCTGAGACGAAACGCGATCTGCTGTTCGTGCTGCTCGATTTGCTCATGTCGC
GTCTGGCGCGCGCCGGGGCAACGGGCCTGCCCCCTACGGTCGAGGCCACGCCCGGCGAGGCGGCGACCTTGATGCGCCTG
TCGCCCGACCCTGCTGCCGCGCGGGCCTGGGCCGAAATCGCGCAGGAAACAAGTGCGCGCGCGCGCCATGGCCTTGCGGT
GAACCTTGACCCTGCGGCTCTGGTCCTAGATACCCTCATCAAGATCAGCCAAACACGTGTGGCCTAG

Upstream 100 bases:

>100_bases
TGGCGCAGGAGTTCAAAGCCCGCTTTCGCCTTGTGGATGGCGGGCGGGATGCCGACAGTGTCGCAGCGGAGGTGATCAAG
ATCGTCGATCAGGCGCTGGC

Downstream 100 bases:

>100_bases
CACACGATGCGCGGCGGCAGTATTGAGCAGACCATGAGCACGCAACCACATATCACCGACAGTCACTGCCACCTCGATTT
CCCTGATTTCGAGGGACGTA

Product: DNA polymerase III subunit delta'

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 368; Mature: 367

Protein sequence:

>368_residues
MTAPDQIEGAPHPRDTPQVFGQATAEQGFLDAYATGRLHHAWLLTGPRGVGKATLAWAIARFLLATPPQEDEGLFGAPPA
PTTLAIDPEHPVARRIAAGAEPGLKHITRSENEKTGRMRDVIAVDDIRGLSSFFQLSATEGGRRVVIVDAADEMNPQAAN
ALLKMLEEPPARATLILISHQPSGLLPTIRSRCRVLRLGTLQPEDMEKALTQAGIDPAGHGPALAELSGGSVGAAVRLSN
FDGLKTYAELVQLIIALPQFDRARALKLAEAAAARGAETKRDLLFVLLDLLMSRLARAGATGLPPTVEATPGEAATLMRL
SPDPAAARAWAEIAQETSARARHGLAVNLDPAALVLDTLIKISQTRVA

Sequences:

>Translated_368_residues
MTAPDQIEGAPHPRDTPQVFGQATAEQGFLDAYATGRLHHAWLLTGPRGVGKATLAWAIARFLLATPPQEDEGLFGAPPA
PTTLAIDPEHPVARRIAAGAEPGLKHITRSENEKTGRMRDVIAVDDIRGLSSFFQLSATEGGRRVVIVDAADEMNPQAAN
ALLKMLEEPPARATLILISHQPSGLLPTIRSRCRVLRLGTLQPEDMEKALTQAGIDPAGHGPALAELSGGSVGAAVRLSN
FDGLKTYAELVQLIIALPQFDRARALKLAEAAAARGAETKRDLLFVLLDLLMSRLARAGATGLPPTVEATPGEAATLMRL
SPDPAAARAWAEIAQETSARARHGLAVNLDPAALVLDTLIKISQTRVA
>Mature_367_residues
TAPDQIEGAPHPRDTPQVFGQATAEQGFLDAYATGRLHHAWLLTGPRGVGKATLAWAIARFLLATPPQEDEGLFGAPPAP
TTLAIDPEHPVARRIAAGAEPGLKHITRSENEKTGRMRDVIAVDDIRGLSSFFQLSATEGGRRVVIVDAADEMNPQAANA
LLKMLEEPPARATLILISHQPSGLLPTIRSRCRVLRLGTLQPEDMEKALTQAGIDPAGHGPALAELSGGSVGAAVRLSNF
DGLKTYAELVQLIIALPQFDRARALKLAEAAAARGAETKRDLLFVLLDLLMSRLARAGATGLPPTVEATPGEAATLMRLS
PDPAAARAWAEIAQETSARARHGLAVNLDPAALVLDTLIKISQTRVA

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity [H]

COG id: COG0470

COG function: function code L; ATPase involved in DNA replication

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786676, Length=309, Percent_Identity=27.831715210356, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1787341, Length=335, Percent_Identity=28.6567164179104, Blast_Score=65, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003959
- InterPro:   IPR004622 [H]

Pfam domain/function: PF00004 AAA [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 38947; Mature: 38816

Theoretical pI: Translated: 6.73; Mature: 6.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAPDQIEGAPHPRDTPQVFGQATAEQGFLDAYATGRLHHAWLLTGPRGVGKATLAWAIA
CCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHCCCEEEEEEEECCCCCCHHHHHHHHH
RFLLATPPQEDEGLFGAPPAPTTLAIDPEHPVARRIAAGAEPGLKHITRSENEKTGRMRD
HHHHCCCCCCCCCCCCCCCCCCEEEECCCCHHHHHHHCCCCCCHHHHHCCCCCCCCHHHH
VIAVDDIRGLSSFFQLSATEGGRRVVIVDAADEMNPQAANALLKMLEEPPARATLILISH
EEEHHHHHHHHHHHHHHCCCCCCEEEEEECCHHCCHHHHHHHHHHHHCCCCCEEEEEEEC
QPSGLLPTIRSRCRVLRLGTLQPEDMEKALTQAGIDPAGHGPALAELSGGSVGAAVRLSN
CCCCCCHHHHHHHHEEEECCCCHHHHHHHHHHCCCCCCCCCCCEEEECCCCCEEEEEECC
FDGLKTYAELVQLIIALPQFDRARALKLAEAAAARGAETKRDLLFVLLDLLMSRLARAGA
CCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCC
TGLPPTVEATPGEAATLMRLSPDPAAARAWAEIAQETSARARHGLAVNLDPAALVLDTLI
CCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCEEECCHHHHHHHHHH
KISQTRVA
HHHHHCCC
>Mature Secondary Structure 
TAPDQIEGAPHPRDTPQVFGQATAEQGFLDAYATGRLHHAWLLTGPRGVGKATLAWAIA
CCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHCCCEEEEEEEECCCCCCHHHHHHHHH
RFLLATPPQEDEGLFGAPPAPTTLAIDPEHPVARRIAAGAEPGLKHITRSENEKTGRMRD
HHHHCCCCCCCCCCCCCCCCCCEEEECCCCHHHHHHHCCCCCCHHHHHCCCCCCCCHHHH
VIAVDDIRGLSSFFQLSATEGGRRVVIVDAADEMNPQAANALLKMLEEPPARATLILISH
EEEHHHHHHHHHHHHHHCCCCCCEEEEEECCHHCCHHHHHHHHHHHHCCCCCEEEEEEEC
QPSGLLPTIRSRCRVLRLGTLQPEDMEKALTQAGIDPAGHGPALAELSGGSVGAAVRLSN
CCCCCCHHHHHHHHEEEECCCCHHHHHHHHHHCCCCCCCCCCCEEEECCCCCEEEEEECC
FDGLKTYAELVQLIIALPQFDRARALKLAEAAAARGAETKRDLLFVLLDLLMSRLARAGA
CCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCC
TGLPPTVEATPGEAATLMRLSPDPAAARAWAEIAQETSARARHGLAVNLDPAALVLDTLI
CCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCEEECCHHHHHHHHHH
KISQTRVA
HHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8550441; 10984043 [H]