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The map label for this gene is ispB [H]

Identifier: 110680572

GI number: 110680572

Start: 3265688

End: 3266689

Strand: Direct

Name: ispB [H]

Synonym: RD1_3401

Alternate gene names: 110680572

Gene position: 3265688-3266689 (Clockwise)

Preceding gene: 110680568

Following gene: 110680576

Centisome position: 79.01

GC content: 58.98

Gene sequence:

>1002_bases
ATGTCAAAAACCGAAAACGCGCAGAAGCCACACGACCGTATGGCGGCCTATCTGGAAAGCGATATGGCGGCGGTGAGTAC
GCTCATTCGTGAGCGCATGGCGTCAAAACATGCCCCACGGATTCCCGAAGTGACGGCGCATCTGGTTGAAGCCGGGGGAA
AACGCCTGCGGCCCATGCTGACGCTGGCTGCGGCGCATCTGTGCGGCTATGACGGCCCGTTTCACATCAACCTTGCGGCA
ACGGTGGAGTTTATCCACACCGCCACCCTTTTGCACGATGACGTGGTGGATGAAAGTGGCCAGCGGCGCGGGCGGCCCAC
GGCGAACCTGCTGTGGGACAATAAATCCTCTGTGCTGGTGGGGGACTATCTGTTTTCACGCAGTTTTCAATTAATGGTCG
AAACCGGATCGCTGCGCGTGCTGGACATCCTCGCCAATGCCTCTGCGACGATTGCGGAGGGCGAGGTTTTGCAACTTACC
GCCAGCCAGAATCTCGCCACCACAGAGGAGGTCTATCTACAGGTCGTGCGCGGCAAGACTGCGGCGTTGTTTTCGGCCGC
GACCGAAGTGGGCGGGGTCATTGCTGGCGCGCCGGATGATCACGTTGCGGCCCTTTTCGAGTATGGCGATGCGCTGGGAA
TTGCTTTTCAGATCGTCGATGATCTGCTCGATTTTCAAGGCGATGCCGGTGCAATCGGCAAAAACATCGGGGATGATTTC
CGCGAACGCAAACTGACCATGCCACTGATCAAAGCGGTCGCCAAAGCGGATGCCGAAGAACGCGCCTTTTGGGTGCGCAC
GATTGAAAAGGGCAAGCAGGAAGAGGGCGATTTGCAACACGCCATCGCCCTGTTGCACAAGCATGCAGCGCTCACGGACA
CAAGCGTTGTCGCGCGCACGCAAGCCGCGCGGGCCAAAGCAGCGCTCGCCCCCCTTCCCGCACATCCGGTCAAGGACATG
CTGGTCGATCTGGCGGATTATGTGGTTGAACGCATCAACTGA

Upstream 100 bases:

>100_bases
AAGGCGATGATGGTCGGGTCTGTGCTGCGCAAAAGCTCTTTCATGTCAGGGATGTAAGGGCAAGCCGGCCGAAATGTCGA
GACTTGCGGACGGGATTTTA

Downstream 100 bases:

>100_bases
GACGGGGTACTGCGCGTTCGGCCTGTGATCCAAGCTGCGTGGGCCTGATCCACCACTGCGGATAATCGCGGGACAAATCG
CGCGCAGCCTGCTGCGCTGC

Product: octaprenyl-diphosphate synthase

Products: NA

Alternate protein names: All-trans-octaprenyl-diphosphate synthase; Octaprenyl pyrophosphate synthase; OPP synthase [H]

Number of amino acids: Translated: 333; Mature: 332

Protein sequence:

>333_residues
MSKTENAQKPHDRMAAYLESDMAAVSTLIRERMASKHAPRIPEVTAHLVEAGGKRLRPMLTLAAAHLCGYDGPFHINLAA
TVEFIHTATLLHDDVVDESGQRRGRPTANLLWDNKSSVLVGDYLFSRSFQLMVETGSLRVLDILANASATIAEGEVLQLT
ASQNLATTEEVYLQVVRGKTAALFSAATEVGGVIAGAPDDHVAALFEYGDALGIAFQIVDDLLDFQGDAGAIGKNIGDDF
RERKLTMPLIKAVAKADAEERAFWVRTIEKGKQEEGDLQHAIALLHKHAALTDTSVVARTQAARAKAALAPLPAHPVKDM
LVDLADYVVERIN

Sequences:

>Translated_333_residues
MSKTENAQKPHDRMAAYLESDMAAVSTLIRERMASKHAPRIPEVTAHLVEAGGKRLRPMLTLAAAHLCGYDGPFHINLAA
TVEFIHTATLLHDDVVDESGQRRGRPTANLLWDNKSSVLVGDYLFSRSFQLMVETGSLRVLDILANASATIAEGEVLQLT
ASQNLATTEEVYLQVVRGKTAALFSAATEVGGVIAGAPDDHVAALFEYGDALGIAFQIVDDLLDFQGDAGAIGKNIGDDF
RERKLTMPLIKAVAKADAEERAFWVRTIEKGKQEEGDLQHAIALLHKHAALTDTSVVARTQAARAKAALAPLPAHPVKDM
LVDLADYVVERIN
>Mature_332_residues
SKTENAQKPHDRMAAYLESDMAAVSTLIRERMASKHAPRIPEVTAHLVEAGGKRLRPMLTLAAAHLCGYDGPFHINLAAT
VEFIHTATLLHDDVVDESGQRRGRPTANLLWDNKSSVLVGDYLFSRSFQLMVETGSLRVLDILANASATIAEGEVLQLTA
SQNLATTEEVYLQVVRGKTAALFSAATEVGGVIAGAPDDHVAALFEYGDALGIAFQIVDDLLDFQGDAGAIGKNIGDDFR
ERKLTMPLIKAVAKADAEERAFWVRTIEKGKQEEGDLQHAIALLHKHAALTDTSVVARTQAARAKAALAPLPAHPVKDML
VDLADYVVERIN

Specific function: Supplies octaprenyl diphosphate, the precursor for the side chain of the isoprenoid quinones ubiquinone and menaquinone [H]

COG id: COG0142

COG function: function code H; Geranylgeranyl pyrophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FPP/GGPP synthase family [H]

Homologues:

Organism=Homo sapiens, GI50659086, Length=299, Percent_Identity=29.0969899665552, Blast_Score=129, Evalue=3e-30,
Organism=Homo sapiens, GI4758430, Length=213, Percent_Identity=25.8215962441315, Blast_Score=69, Evalue=6e-12,
Organism=Homo sapiens, GI83700220, Length=213, Percent_Identity=25.8215962441315, Blast_Score=69, Evalue=6e-12,
Organism=Escherichia coli, GI1789578, Length=312, Percent_Identity=43.2692307692308, Blast_Score=249, Evalue=2e-67,
Organism=Escherichia coli, GI1786623, Length=226, Percent_Identity=33.1858407079646, Blast_Score=87, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17505681, Length=244, Percent_Identity=32.3770491803279, Blast_Score=133, Evalue=1e-31,
Organism=Saccharomyces cerevisiae, GI6319475, Length=270, Percent_Identity=29.6296296296296, Blast_Score=119, Evalue=1e-27,
Organism=Saccharomyces cerevisiae, GI6325188, Length=257, Percent_Identity=25.2918287937743, Blast_Score=80, Evalue=6e-16,
Organism=Drosophila melanogaster, GI24651612, Length=307, Percent_Identity=30.6188925081433, Blast_Score=143, Evalue=2e-34,
Organism=Drosophila melanogaster, GI281365769, Length=283, Percent_Identity=22.6148409893993, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24660002, Length=283, Percent_Identity=22.6148409893993, Blast_Score=69, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000092
- InterPro:   IPR017446
- InterPro:   IPR008949 [H]

Pfam domain/function: PF00348 polyprenyl_synt [H]

EC number: =2.5.1.90 [H]

Molecular weight: Translated: 35991; Mature: 35860

Theoretical pI: Translated: 5.75; Mature: 5.75

Prosite motif: PS00723 POLYPRENYL_SYNTHET_1 ; PS00444 POLYPRENYL_SYNTHET_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKTENAQKPHDRMAAYLESDMAAVSTLIRERMASKHAPRIPEVTAHLVEAGGKRLRPML
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCHHHHHHH
TLAAAHLCGYDGPFHINLAATVEFIHTATLLHDDVVDESGQRRGRPTANLLWDNKSSVLV
HHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHCCCCHHCCCCCCEEEECCCCCEEE
GDYLFSRSFQLMVETGSLRVLDILANASATIAEGEVLQLTASQNLATTEEVYLQVVRGKT
HHHHHCCCEEEEEECCCEEEEEEHHCCCCEECCCCEEEEECCCCCCHHHHHHHHHHCCCH
AALFSAATEVGGVIAGAPDDHVAALFEYGDALGIAFQIVDDLLDFQGDAGAIGKNIGDDF
HHHHHHHHHHCCEEECCCCHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHH
RERKLTMPLIKAVAKADAEERAFWVRTIEKGKQEEGDLQHAIALLHKHAALTDTSVVART
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCHHHHHHHH
QAARAKAALAPLPAHPVKDMLVDLADYVVERIN
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SKTENAQKPHDRMAAYLESDMAAVSTLIRERMASKHAPRIPEVTAHLVEAGGKRLRPML
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCHHHHHHH
TLAAAHLCGYDGPFHINLAATVEFIHTATLLHDDVVDESGQRRGRPTANLLWDNKSSVLV
HHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHCCCCHHCCCCCCEEEECCCCCEEE
GDYLFSRSFQLMVETGSLRVLDILANASATIAEGEVLQLTASQNLATTEEVYLQVVRGKT
HHHHHCCCEEEEEECCCEEEEEEHHCCCCEECCCCEEEEECCCCCCHHHHHHHHHHCCCH
AALFSAATEVGGVIAGAPDDHVAALFEYGDALGIAFQIVDDLLDFQGDAGAIGKNIGDDF
HHHHHHHHHHCCEEECCCCHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHH
RERKLTMPLIKAVAKADAEERAFWVRTIEKGKQEEGDLQHAIALLHKHAALTDTSVVART
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCHHHHHHHH
QAARAKAALAPLPAHPVKDMLVDLADYVVERIN
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8312607; 9278503; 2670911; 8037730 [H]