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The map label for this gene is gcvA [H]

Identifier: 110680568

GI number: 110680568

Start: 3263415

End: 3264347

Strand: Direct

Name: gcvA [H]

Synonym: RD1_3397

Alternate gene names: 110680568

Gene position: 3263415-3264347 (Clockwise)

Preceding gene: 110680566

Following gene: 110680572

Centisome position: 78.96

GC content: 61.63

Gene sequence:

>933_bases
ATGAACGATCGACTCCCGCCCCTCACTGCACTGCGTGCCTTTGATGCCGCCGCCCGACATATGTCATTTGCCAAAGCGGC
GGATGAGCTATCCGTGACACCTGCCGCACTGAGCTTTCAGATCAAATCCCTTGAGGCACATCTGGGTGCGCCCTTGTTTC
GCCGTCTTAACCGGGCCGTCGCCTTAACCGAAGCCGGGCAAGTGCTCGCCCCCGGTGCGACGGACGGGTTTGCAACGCTC
TTGGCAGCATGGCGTGCCGCACAGCGTTTGCAGGACGAACAGACCCTGACGGTCACGGCGGGCCCTGCGTTTACGGCCAA
ATGGCTTGCGCCGCGCCTTTATGAATTTGCCCAAGCCCATCCGGAGATCGAACTTCGCTTCTCTGCGTCGCTGAAGATCG
TCGACTTTGCGCGGGACGCCGTTGATGTTGCCGTCCGTTTCGGTCCGCGGGTCGATCCGGCGCTGTGGGCCCTGCCGCTG
GCCAAGGAATGGGTCGCACCGGTGATGACACCGGCCCTGGCGCAGAAATACCCGACCCCTGAAAGCCTGACCAAAGCGCC
GCTGATCTTTGACGATTCGATCAGTTTCCTGCAGCCTGTCCCGGATTGGGCGGCATGGTTCCGCACGATGGGCGTCGAAT
TCAGGCCCGACCACGGTCCGCGATTCAGTCAGGCGGATCATGCGATAGACGCGGCACTTGCGGGTGTTGGTGTGGTGCTT
GGGCGCCGCGCCTTGGTGGTAAAGGACATTGACGAAGGTCGCCTTGTGCTGCCCTTTCCGCGCGCCATGCACACGGGGGC
GCGGTTTCACTTTCTCTGCACACGCGGCACCGAAGACAGGCCGCAAGTGCGCGCGTTCCGCGAATGGATGATCGCGGAAA
TTGCAAAAACGGCTTACATTACGGAGGGTTTGGAGATTGTGCCAATCGCCTGA

Upstream 100 bases:

>100_bases
TTCAGCTTCAGTGTTTCTGAACTGATACAAAGATGACACTTTGGCAGCAAAGCCACAAACGAGACTTTCGCTGGGTTCAG
TTAAGTATTACTTATGCGAA

Downstream 100 bases:

>100_bases
GGGCGGATCAGGGCAACACGGTCGCCAAACCGCGTGCCCGTCGCATTGTTTCTGTCTAGTTTGACAGTCGGGTGATTTCT
TCTTTGAGGCGAAGTTTTTG

Product: DNA-binding transcriptional activator GcvA

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 310; Mature: 310

Protein sequence:

>310_residues
MNDRLPPLTALRAFDAAARHMSFAKAADELSVTPAALSFQIKSLEAHLGAPLFRRLNRAVALTEAGQVLAPGATDGFATL
LAAWRAAQRLQDEQTLTVTAGPAFTAKWLAPRLYEFAQAHPEIELRFSASLKIVDFARDAVDVAVRFGPRVDPALWALPL
AKEWVAPVMTPALAQKYPTPESLTKAPLIFDDSISFLQPVPDWAAWFRTMGVEFRPDHGPRFSQADHAIDAALAGVGVVL
GRRALVVKDIDEGRLVLPFPRAMHTGARFHFLCTRGTEDRPQVRAFREWMIAEIAKTAYITEGLEIVPIA

Sequences:

>Translated_310_residues
MNDRLPPLTALRAFDAAARHMSFAKAADELSVTPAALSFQIKSLEAHLGAPLFRRLNRAVALTEAGQVLAPGATDGFATL
LAAWRAAQRLQDEQTLTVTAGPAFTAKWLAPRLYEFAQAHPEIELRFSASLKIVDFARDAVDVAVRFGPRVDPALWALPL
AKEWVAPVMTPALAQKYPTPESLTKAPLIFDDSISFLQPVPDWAAWFRTMGVEFRPDHGPRFSQADHAIDAALAGVGVVL
GRRALVVKDIDEGRLVLPFPRAMHTGARFHFLCTRGTEDRPQVRAFREWMIAEIAKTAYITEGLEIVPIA
>Mature_310_residues
MNDRLPPLTALRAFDAAARHMSFAKAADELSVTPAALSFQIKSLEAHLGAPLFRRLNRAVALTEAGQVLAPGATDGFATL
LAAWRAAQRLQDEQTLTVTAGPAFTAKWLAPRLYEFAQAHPEIELRFSASLKIVDFARDAVDVAVRFGPRVDPALWALPL
AKEWVAPVMTPALAQKYPTPESLTKAPLIFDDSISFLQPVPDWAAWFRTMGVEFRPDHGPRFSQADHAIDAALAGVGVVL
GRRALVVKDIDEGRLVLPFPRAMHTGARFHFLCTRGTEDRPQVRAFREWMIAEIAKTAYITEGLEIVPIA

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=299, Percent_Identity=39.7993311036789, Blast_Score=195, Evalue=3e-51,
Organism=Escherichia coli, GI1786448, Length=294, Percent_Identity=33.3333333333333, Blast_Score=145, Evalue=2e-36,
Organism=Escherichia coli, GI1788706, Length=295, Percent_Identity=30.1694915254237, Blast_Score=134, Evalue=8e-33,
Organism=Escherichia coli, GI157672245, Length=170, Percent_Identity=37.6470588235294, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI145693193, Length=296, Percent_Identity=25, Blast_Score=85, Evalue=5e-18,
Organism=Escherichia coli, GI87081978, Length=189, Percent_Identity=32.2751322751323, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1787589, Length=290, Percent_Identity=26.551724137931, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1786401, Length=255, Percent_Identity=25.0980392156863, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1787128, Length=255, Percent_Identity=27.0588235294118, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1789440, Length=251, Percent_Identity=26.2948207171315, Blast_Score=69, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34053; Mature: 34053

Theoretical pI: Translated: 7.81; Mature: 7.81

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDRLPPLTALRAFDAAARHMSFAKAADELSVTPAALSFQIKSLEAHLGAPLFRRLNRAV
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHEEHHHHHHHHCCHHHHHHHHHE
ALTEAGQVLAPGATDGFATLLAAWRAAQRLQDEQTLTVTAGPAFTAKWLAPRLYEFAQAH
EEECCCCEECCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHCC
PEIELRFSASLKIVDFARDAVDVAVRFGPRVDPALWALPLAKEWVAPVMTPALAQKYPTP
CCEEEEEECCEEEEHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCHHHHHCCCCC
ESLTKAPLIFDDSISFLQPVPDWAAWFRTMGVEFRPDHGPRFSQADHAIDAALAGVGVVL
HHHCCCCEEECCCCHHHCCCCHHHHHHHHCCCEECCCCCCCCCHHHHHHHHHHHHHHHHH
GRRALVVKDIDEGRLVLPFPRAMHTGARFHFLCTRGTEDRPQVRAFREWMIAEIAKTAYI
CCCEEEEEECCCCEEEECCCHHHHCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHH
TEGLEIVPIA
HCCCEEEECC
>Mature Secondary Structure
MNDRLPPLTALRAFDAAARHMSFAKAADELSVTPAALSFQIKSLEAHLGAPLFRRLNRAV
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHEEHHHHHHHHCCHHHHHHHHHE
ALTEAGQVLAPGATDGFATLLAAWRAAQRLQDEQTLTVTAGPAFTAKWLAPRLYEFAQAH
EEECCCCEECCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHCC
PEIELRFSASLKIVDFARDAVDVAVRFGPRVDPALWALPLAKEWVAPVMTPALAQKYPTP
CCEEEEEECCEEEEHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCHHHHHCCCCC
ESLTKAPLIFDDSISFLQPVPDWAAWFRTMGVEFRPDHGPRFSQADHAIDAALAGVGVVL
HHHCCCCEEECCCCHHHCCCCHHHHHHHHCCCEECCCCCCCCCHHHHHHHHHHHHHHHHH
GRRALVVKDIDEGRLVLPFPRAMHTGARFHFLCTRGTEDRPQVRAFREWMIAEIAKTAYI
CCCEEEEEECCCCEEEECCCHHHHCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHH
TEGLEIVPIA
HCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]