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The map label for this gene is greA [H]

Identifier: 110680576

GI number: 110680576

Start: 3271168

End: 3272106

Strand: Direct

Name: greA [H]

Synonym: RD1_3405

Alternate gene names: 110680576

Gene position: 3271168-3272106 (Clockwise)

Preceding gene: 110680572

Following gene: 110680577

Centisome position: 79.15

GC content: 59.85

Gene sequence:

>939_bases
TTGGTGCAATGCAATCGCTACACGGCGTCAAAATGTATCTTTGGCGCGGATCAACAACCTGCGCGCCACCCAAGCGTGCG
AAATCCGATCACTCCGGCGGCGCGCTCATCAGACACCCGGGGCCGACGCCCTTTGTCGCGGCCTTCTTCTTCGGGGTACA
CAGCGCACAGCGTGGCAGGCCCGGGCCGCCACATCCGTCAAGGTCAACGCGCAGGCCGGTCAACCAAGCGAGCTTGTGAT
GCAATGCCATGCGAAACCGCGCACGGTACGCGAACGCGGGTCGGATTGAACATGAAACCCTACAATATGACCGATGGCGC
GGGTCACGGGAAATTTTCCGCCGAACGGCAGCCCCCGCAGGGTCGACTCCCCTTGCAATCTGCCGCGCCTTTGGGTCAGG
TAGGGTGCAATTGTCACCAGCGGCCCCCGGACTCCCCGGCGGGCCGCCGTATTTCGTGGATACCGCCAATGGATAAGATA
CCCATGACCCGCAAGGGCCACTCCGATCTTGAAGCCGAGCTGAAGAACCTGAAGTCTGTTGAGCGCCCGGCCATCATAAG
TGCCATCGCAGAAGCGCGCGAGCACGGTGATCTGTCCGAGAATGCCGAATATCATTCGGCCCGCGAAAAGCACTCGTTCA
TTGAAGGCCGGATCAAGGAACTCGAAGGCGTGCTGGGTCTGGCCGAGGTGATTGATCCATCAAAGATGTCCGGCGCGATC
AAATTCGGCGCGACCGTCACTCTGGTGGACGAAGACACCGACGAAGAGAAAACCTGGCAGATTGTCGGCGAGCATGAGGC
GAATGTCGAAAAAGGCTTGCTCAACATCAAATCGCCCATTGCGCGGGCGCTGATTGGCAAGGACGAAGGTGACAGCGTCG
AGGTGCGCACGCCGGGCGGGCAAAAGGCCTATGAGGTGTTGAAAATCGTCTATGCCTGA

Upstream 100 bases:

>100_bases
TTGTTTCGCGTGGCGTATCGGTCATGGACGATTCCCCTGTCAATTTCCCATTCGGCGTTATGCCAAGTAAAATAAAGCTC
GCGATTGGCTAAACCACGTC

Downstream 100 bases:

>100_bases
CAGGACCCCGTGATATGGCCGATGCCAGCGAACAAGACCCACAGACCGGCGCGGCGCATACGCGACCGACACCCTTGGGC
ATATATGATCGTCCCAAAAC

Product: transcription elongation factor GreA

Products: NA

Alternate protein names: Transcript cleavage factor greA [H]

Number of amino acids: Translated: 312; Mature: 312

Protein sequence:

>312_residues
MVQCNRYTASKCIFGADQQPARHPSVRNPITPAARSSDTRGRRPLSRPSSSGYTAHSVAGPGRHIRQGQRAGRSTKRACD
AMPCETAHGTRTRVGLNMKPYNMTDGAGHGKFSAERQPPQGRLPLQSAAPLGQVGCNCHQRPPDSPAGRRISWIPPMDKI
PMTRKGHSDLEAELKNLKSVERPAIISAIAEAREHGDLSENAEYHSAREKHSFIEGRIKELEGVLGLAEVIDPSKMSGAI
KFGATVTLVDEDTDEEKTWQIVGEHEANVEKGLLNIKSPIARALIGKDEGDSVEVRTPGGQKAYEVLKIVYA

Sequences:

>Translated_312_residues
MVQCNRYTASKCIFGADQQPARHPSVRNPITPAARSSDTRGRRPLSRPSSSGYTAHSVAGPGRHIRQGQRAGRSTKRACD
AMPCETAHGTRTRVGLNMKPYNMTDGAGHGKFSAERQPPQGRLPLQSAAPLGQVGCNCHQRPPDSPAGRRISWIPPMDKI
PMTRKGHSDLEAELKNLKSVERPAIISAIAEAREHGDLSENAEYHSAREKHSFIEGRIKELEGVLGLAEVIDPSKMSGAI
KFGATVTLVDEDTDEEKTWQIVGEHEANVEKGLLNIKSPIARALIGKDEGDSVEVRTPGGQKAYEVLKIVYA
>Mature_312_residues
MVQCNRYTASKCIFGADQQPARHPSVRNPITPAARSSDTRGRRPLSRPSSSGYTAHSVAGPGRHIRQGQRAGRSTKRACD
AMPCETAHGTRTRVGLNMKPYNMTDGAGHGKFSAERQPPQGRLPLQSAAPLGQVGCNCHQRPPDSPAGRRISWIPPMDKI
PMTRKGHSDLEAELKNLKSVERPAIISAIAEAREHGDLSENAEYHSAREKHSFIEGRIKELEGVLGLAEVIDPSKMSGAI
KFGATVTLVDEDTDEEKTWQIVGEHEANVEKGLLNIKSPIARALIGKDEGDSVEVRTPGGQKAYEVLKIVYA

Specific function: Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary

COG id: COG0782

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the greA/greB family [H]

Homologues:

Organism=Escherichia coli, GI87082225, Length=157, Percent_Identity=56.687898089172, Blast_Score=180, Evalue=1e-46,
Organism=Escherichia coli, GI87082261, Length=156, Percent_Identity=34.6153846153846, Blast_Score=82, Evalue=7e-17,

Paralogues:

None

Copy number: 259 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018151
- InterPro:   IPR006359
- InterPro:   IPR001437
- InterPro:   IPR022691 [H]

Pfam domain/function: PF01272 GreA_GreB; PF03449 GreA_GreB_N [H]

EC number: NA

Molecular weight: Translated: 33919; Mature: 33919

Theoretical pI: Translated: 9.42; Mature: 9.42

Prosite motif: PS00829 GREAB_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVQCNRYTASKCIFGADQQPARHPSVRNPITPAARSSDTRGRRPLSRPSSSGYTAHSVAG
CCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCC
PGRHIRQGQRAGRSTKRACDAMPCETAHGTRTRVGLNMKPYNMTDGAGHGKFSAERQPPQ
CHHHHHHHHHHCHHHHHHHHCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCC
GRLPLQSAAPLGQVGCNCHQRPPDSPAGRRISWIPPMDKIPMTRKGHSDLEAELKNLKSV
CCCCCHHCCCHHHHCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHH
ERPAIISAIAEAREHGDLSENAEYHSAREKHSFIEGRIKELEGVLGLAEVIDPSKMSGAI
CCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCEE
KFGATVTLVDEDTDEEKTWQIVGEHEANVEKGLLNIKSPIARALIGKDEGDSVEVRTPGG
EECCEEEEEECCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCC
QKAYEVLKIVYA
HHHHHHHHHHCC
>Mature Secondary Structure
MVQCNRYTASKCIFGADQQPARHPSVRNPITPAARSSDTRGRRPLSRPSSSGYTAHSVAG
CCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCC
PGRHIRQGQRAGRSTKRACDAMPCETAHGTRTRVGLNMKPYNMTDGAGHGKFSAERQPPQ
CHHHHHHHHHHCHHHHHHHHCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCC
GRLPLQSAAPLGQVGCNCHQRPPDSPAGRRISWIPPMDKIPMTRKGHSDLEAELKNLKSV
CCCCCHHCCCHHHHCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHH
ERPAIISAIAEAREHGDLSENAEYHSAREKHSFIEGRIKELEGVLGLAEVIDPSKMSGAI
CCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCEE
KFGATVTLVDEDTDEEKTWQIVGEHEANVEKGLLNIKSPIARALIGKDEGDSVEVRTPGG
EECCEEEEEECCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCC
QKAYEVLKIVYA
HHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA