Click here to switch to the map view.

The map label for this gene is ispF

Identifier: 110679977

GI number: 110679977

Start: 2640503

End: 2640982

Strand: Direct

Name: ispF

Synonym: RD1_2767

Alternate gene names: 110679977

Gene position: 2640503-2640982 (Clockwise)

Preceding gene: 110679976

Following gene: 110679978

Centisome position: 63.89

GC content: 59.79

Gene sequence:

>480_bases
ATGGACATTAGACTTGGCAACGGTTTTGACGTGCATGCTTTCGGGCCCGGCGATCATGTGACGCTTTGCGGTGTGAAAAT
ACCCCATGGGCAGGGGCTGGTTGGGCATTCAGACGCGGATGTGGCGATGCATACAGTGACGGACGCGATCTATGGCGCGC
TGGCGATGGGCGATATCGGCCAGCATTTCCCGCCGAGTGATCCGCAGTGGAAAGGGGCCGCATCCGATATCTTTCTGAAA
CATGCGGTGGCGCAGGCAAGTGAACAGGGGTTTGAAATCAATAACGTGGATTGCACGCTTATCTGTGAGTTCCCCAAGAT
CGGCCCGCATGCACAGGCCATGCGTGAGGCGATGGCCCGGATTATGGGCATGGCCCTGTCGCGCATCTCCGTTAAGGCGA
CCACGTCGGAACGGCTTGGCTTTACCGGCCGTGGTGAGGGGATCGCCTGCATCGCCACCGCGGCCCTGGTGCGCGCATGA

Upstream 100 bases:

>100_bases
GCCGGGCTTGACGTTGTCATCGTGCCCGGAGATGAAGATAACCTGAAAATAACGCTGCCCGGCGACTTTGCCCGGGCGGA
GGCAATCCTGAGGGCCCGCG

Downstream 100 bases:

>100_bases
GCGGTCTGGCCAAGATGATCGCCACCGTCCTTGGCGTGGGATATGTGCGCCCTGCCCCCGGCACATGGGGCTCTCTCGTG
GCGCTGCCTTGGGCGTGGCT

Product: 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Products: NA

Alternate protein names: MECDP-synthase; MECPS

Number of amino acids: Translated: 159; Mature: 159

Protein sequence:

>159_residues
MDIRLGNGFDVHAFGPGDHVTLCGVKIPHGQGLVGHSDADVAMHTVTDAIYGALAMGDIGQHFPPSDPQWKGAASDIFLK
HAVAQASEQGFEINNVDCTLICEFPKIGPHAQAMREAMARIMGMALSRISVKATTSERLGFTGRGEGIACIATAALVRA

Sequences:

>Translated_159_residues
MDIRLGNGFDVHAFGPGDHVTLCGVKIPHGQGLVGHSDADVAMHTVTDAIYGALAMGDIGQHFPPSDPQWKGAASDIFLK
HAVAQASEQGFEINNVDCTLICEFPKIGPHAQAMREAMARIMGMALSRISVKATTSERLGFTGRGEGIACIATAALVRA
>Mature_159_residues
MDIRLGNGFDVHAFGPGDHVTLCGVKIPHGQGLVGHSDADVAMHTVTDAIYGALAMGDIGQHFPPSDPQWKGAASDIFLK
HAVAQASEQGFEINNVDCTLICEFPKIGPHAQAMREAMARIMGMALSRISVKATTSERLGFTGRGEGIACIATAALVRA

Specific function: Converts 4-diphosphocytidyl-2-C-methyl-D-erythritol 2- phosphate into 2-C-methyl-D-erythritol 2,4-cyclodiphosphate (MECDP) and CMP

COG id: COG0245

COG function: function code I; 2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispF family

Homologues:

Organism=Escherichia coli, GI1789103, Length=157, Percent_Identity=50.3184713375796, Blast_Score=157, Evalue=3e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPF_ROSDO (Q165P5)

Other databases:

- EMBL:   CP000362
- RefSeq:   YP_682984.1
- ProteinModelPortal:   Q165P5
- SMR:   Q165P5
- STRING:   Q165P5
- GeneID:   4195925
- GenomeReviews:   CP000362_GR
- KEGG:   rde:RD1_2767
- NMPDR:   fig|375451.6.peg.2566
- eggNOG:   COG0245
- HOGENOM:   HBG630070
- OMA:   HKFGGEG
- PhylomeDB:   Q165P5
- ProtClustDB:   PRK00084
- BioCyc:   RDEN375451:RD1_2767-MONOMER
- HAMAP:   MF_00107
- InterPro:   IPR003526
- InterPro:   IPR020555
- Gene3D:   G3DSA:3.30.1330.50
- TIGRFAMs:   TIGR00151

Pfam domain/function: PF02542 YgbB; SSF69765 YgbB_synth

EC number: =4.6.1.12

Molecular weight: Translated: 16710; Mature: 16710

Theoretical pI: Translated: 6.50; Mature: 6.50

Prosite motif: PS01350 ISPF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
6.9 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
6.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDIRLGNGFDVHAFGPGDHVTLCGVKIPHGQGLVGHSDADVAMHTVTDAIYGALAMGDIG
CCEECCCCEEEEEECCCCEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCC
QHFPPSDPQWKGAASDIFLKHAVAQASEQGFEINNVDCTLICEFPKIGPHAQAMREAMAR
CCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEECCCCEEEEECCCCCCCHHHHHHHHHHH
IMGMALSRISVKATTSERLGFTGRGEGIACIATAALVRA
HHHHHHHHEEEEECCHHHCCCCCCCCCCHHHHHHHHHCC
>Mature Secondary Structure
MDIRLGNGFDVHAFGPGDHVTLCGVKIPHGQGLVGHSDADVAMHTVTDAIYGALAMGDIG
CCEECCCCEEEEEECCCCEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCC
QHFPPSDPQWKGAASDIFLKHAVAQASEQGFEINNVDCTLICEFPKIGPHAQAMREAMAR
CCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEECCCCEEEEECCCCCCCHHHHHHHHHHH
IMGMALSRISVKATTSERLGFTGRGEGIACIATAALVRA
HHHHHHHHEEEEECCHHHCCCCCCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA