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The map label for this gene is ispD

Identifier: 110679976

GI number: 110679976

Start: 2639848

End: 2640513

Strand: Direct

Name: ispD

Synonym: RD1_2766

Alternate gene names: 110679976

Gene position: 2639848-2640513 (Clockwise)

Preceding gene: 110679970

Following gene: 110679977

Centisome position: 63.87

GC content: 63.51

Gene sequence:

>666_bases
ATGAAGGTTGGTGCGGTCATTGTCGCGGCGGGGCGCGGAGTACGAGCGGGGGGCGGCATTCCCAAGCAGTGGCGCGCTTT
GCACAAGGGGACAGTCGCACAAGCATCCATTCGCGCCTTCACCGGACATGCGGATATCCGTGACGTCGTGCTGGTGCTGC
ATCCTGATGACATTGACACGGATCTCTGGCCGCGCGAACCCGGGCTGATTGTTGCCAGCGGTGGCGCATCACGCAGCGCA
TCGGTGCTTGCAGGTTTGCGCATGCTTAACGGAAAAACAGATGCGGTGCTGATCCATGATGCCGCCCGGCCCTGCGTGAC
CACCCGCGTGATTGATGATGTCATCAGTGCGCTGCGCACGGCACAGGCCGCCGCGCCCGCTGTCGCCGTGGTCGACGCCT
TGTGGACCGGTGAGAACGGACAGGTCACGGGCACCGCAGACCGGACCGGGCTTTATCGCGCGCAGACGCCGCAGGGCTTT
CACCTTGATGCGATCATTAAAGCGCACCGGCAGTTTCCCGAAGGCGCAGCCGATGACGTGGAGGTTGCACGCCGCGCCGG
GCTTGACGTTGTCATCGTGCCCGGAGATGAAGATAACCTGAAAATAACGCTGCCCGGCGACTTTGCCCGGGCGGAGGCAA
TCCTGAGGGCCCGCGATGGACATTAG

Upstream 100 bases:

>100_bases
CGCGGTTTACCTGTTTGCCTAAATTTAAGGCACCGCTCACAATGATTGCGCAGCGCAGCACAAAGCCATAAACACATGTC
AGGAACGAGGCGGGCATACC

Downstream 100 bases:

>100_bases
ACTTGGCAACGGTTTTGACGTGCATGCTTTCGGGCCCGGCGATCATGTGACGCTTTGCGGTGTGAAAATACCCCATGGGC
AGGGGCTGGTTGGGCATTCA

Product: 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase, putative

Products: NA

Alternate protein names: 4-diphosphocytidyl-2C-methyl-D-erythritol synthase; MEP cytidylyltransferase; MCT

Number of amino acids: Translated: 221; Mature: 221

Protein sequence:

>221_residues
MKVGAVIVAAGRGVRAGGGIPKQWRALHKGTVAQASIRAFTGHADIRDVVLVLHPDDIDTDLWPREPGLIVASGGASRSA
SVLAGLRMLNGKTDAVLIHDAARPCVTTRVIDDVISALRTAQAAAPAVAVVDALWTGENGQVTGTADRTGLYRAQTPQGF
HLDAIIKAHRQFPEGAADDVEVARRAGLDVVIVPGDEDNLKITLPGDFARAEAILRARDGH

Sequences:

>Translated_221_residues
MKVGAVIVAAGRGVRAGGGIPKQWRALHKGTVAQASIRAFTGHADIRDVVLVLHPDDIDTDLWPREPGLIVASGGASRSA
SVLAGLRMLNGKTDAVLIHDAARPCVTTRVIDDVISALRTAQAAAPAVAVVDALWTGENGQVTGTADRTGLYRAQTPQGF
HLDAIIKAHRQFPEGAADDVEVARRAGLDVVIVPGDEDNLKITLPGDFARAEAILRARDGH
>Mature_221_residues
MKVGAVIVAAGRGVRAGGGIPKQWRALHKGTVAQASIRAFTGHADIRDVVLVLHPDDIDTDLWPREPGLIVASGGASRSA
SVLAGLRMLNGKTDAVLIHDAARPCVTTRVIDDVISALRTAQAAAPAVAVVDALWTGENGQVTGTADRTGLYRAQTPQGF
HLDAIIKAHRQFPEGAADDVEVARRAGLDVVIVPGDEDNLKITLPGDFARAEAILRARDGH

Specific function: Catalyzes the formation of 4-diphosphocytidyl-2-C- methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4- phosphate (MEP)

COG id: COG1211

COG function: function code I; 4-diphosphocytidyl-2-methyl-D-erithritol synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispD family

Homologues:

Organism=Escherichia coli, GI1789104, Length=226, Percent_Identity=34.070796460177, Blast_Score=99, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPD_ROSDO (Q165P6)

Other databases:

- EMBL:   CP000362
- RefSeq:   YP_682983.1
- ProteinModelPortal:   Q165P6
- SMR:   Q165P6
- STRING:   Q165P6
- GeneID:   4195924
- GenomeReviews:   CP000362_GR
- KEGG:   rde:RD1_2766
- NMPDR:   fig|375451.6.peg.2565
- eggNOG:   COG1211
- HOGENOM:   HBG672839
- OMA:   SKVIVVC
- PhylomeDB:   Q165P6
- ProtClustDB:   CLSK841056
- BioCyc:   RDEN375451:RD1_2766-MONOMER
- HAMAP:   MF_00108
- InterPro:   IPR001228
- InterPro:   IPR018294
- TIGRFAMs:   TIGR00453

Pfam domain/function: PF01128 IspD

EC number: =2.7.7.60

Molecular weight: Translated: 23174; Mature: 23174

Theoretical pI: Translated: 7.24; Mature: 7.24

Prosite motif: PS01295 ISPD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVGAVIVAAGRGVRAGGGIPKQWRALHKGTVAQASIRAFTGHADIRDVVLVLHPDDIDT
CCCCEEEEECCCCEECCCCCCHHHHHHHCCCHHHHHHEEEECCCCCEEEEEEECCCCCCC
DLWPREPGLIVASGGASRSASVLAGLRMLNGKTDAVLIHDAARPCVTTRVIDDVISALRT
CCCCCCCCEEEECCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHHH
AQAAAPAVAVVDALWTGENGQVTGTADRTGLYRAQTPQGFHLDAIIKAHRQFPEGAADDV
HHHCCCHHEEEHHEEECCCCEEEECCCCCCEEECCCCCCCCHHHHHHHHHHCCCCCCHHH
EVARRAGLDVVIVPGDEDNLKITLPGDFARAEAILRARDGH
HHHHHCCCEEEEEECCCCCEEEEECCCHHHHHHHHHCCCCC
>Mature Secondary Structure
MKVGAVIVAAGRGVRAGGGIPKQWRALHKGTVAQASIRAFTGHADIRDVVLVLHPDDIDT
CCCCEEEEECCCCEECCCCCCHHHHHHHCCCHHHHHHEEEECCCCCEEEEEEECCCCCCC
DLWPREPGLIVASGGASRSASVLAGLRMLNGKTDAVLIHDAARPCVTTRVIDDVISALRT
CCCCCCCCEEEECCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHHH
AQAAAPAVAVVDALWTGENGQVTGTADRTGLYRAQTPQGFHLDAIIKAHRQFPEGAADDV
HHHCCCHHEEEHHEEECCCCEEEECCCCCCEEECCCCCCCCHHHHHHHHHHCCCCCCHHH
EVARRAGLDVVIVPGDEDNLKITLPGDFARAEAILRARDGH
HHHHHCCCEEEEEECCCCCEEEEECCCHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA